FastQCFastQC Report
Sat 31 Dec 2022
SRR5846986.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846986.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147035
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGC5250.35705784337062607No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGCCGTCT5050.34345563981364985TruSeq Adapter, Index 2 (95% over 21bp)
CTGACTGGGTGGTTATGGATCAGGAGACAGTGAAGATGAGAGGAGTGACAGA2640.17954908695208624No Hit
AATGCCGGGTGCCCTTTGATGCTTGATTCCAGTGATTGATTCCCTTTGTTGC2300.15642534090522664No Hit
CAGCCTCGGGGGAGAAGAGAACTGTTCTACACAATAAAAAATTATTGAGAAA2290.15574523072737784No Hit
CACTACGGGGAATAAGAAATTATTTCTCAGAGGAAAGTTAAAGGAAAGTGAT2150.1462236882374945No Hit
TCAGCAGGGTAGCTTAAGATTTTAAGAGAAAATATGAAGACTTAGAAGAGTA2010.1367021457476111No Hit
ACTTGCGGGGAGCTGACCATGGAAGCGAATGGGTTGGGACCTCAGGGTTTTC2000.13602203556976228No Hit
TCGCAGGGAGGGTAAAGTGTATACAATCAAAGTTAAGTTGTAATCAGCTTAA1990.13534192539191348No Hit
TGTTTGGGGGGACATCTTCAGGGAGTTATATCGCTCAGGGAACTAACATCTT1820.12378005236848369No Hit
ATAACAGGGTAGGAAAAAAGGGTATGAAAATAAAATGGAGAGTGAGAGGTAT1820.12378005236848369No Hit
ATTCCAGGGTCACACATTCCATACACACACACATTCCATACGCACAAACACA1790.12173972183493725No Hit
GCAAACGGGTCTGCGTACGGGATAGCAGCTTTATTCATAATTGCCAAAACTT1790.12173972183493725No Hit
ACTTCAGGGATCCAGAGTTACCAAATCTAGTATTTGAAGTCCTTAGGTTTGA1780.12105961165708845No Hit
ATCAGAGGGAGAGCAAAAGAGGCTTGAAGAATATGAAAATAACCAGCCACAG1780.12105961165708845No Hit
AATAAGGGGATCAAAAAGTTGAAATTATATGAAAGTCAGAGAAATGTGAACG1770.12037950147923963No Hit
CTCTCCTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1750.119019281123542No Hit
CGCGCGGGCCGATCCAGAGAGGGAGGAATGAGGGTCGTGACCAACTCAGTAT1740.1183391709456932No Hit
CGACTTGGGCTGCCTGGCTCCTGTAGCATCTGAAAGCCCTGAAGGAACTTGA1720.11697895058999559No Hit
GCATTGGGGCTTCCTTTCCAACTTGGACGCTGCAGAATGGCTCCCGCAAAGA1710.11629884041214676No Hit
ACGATAGGGGCGACGGTCGCCGCGTTCCATCGTCGCGCGGCCCTTCGGGCGC1700.11561873023429796No Hit
TTACCGGGGCTTGATTCCAGTGATTGATTCCCTTTGTTGCTTGAGGGCCATA1690.11493862005644916No Hit
ATTGCAGGGAGTTTGGCATTTTTGGCCCTTTTCAATACTAATCAAACTTACT1690.11493862005644916No Hit
ATCGGGGGTTGTAGATTGGTTATTTCTGTACTTTATCTGTAATAAACTTTGT1680.11425850987860033No Hit
GTACCAGGGGGAAGAGCTCTCGGCTGTGCAGTGGTACCGTCGGGGCCTGGGC1680.11425850987860033No Hit
CTGTGCGGGCCTACTGGAGGATTTATTTAAATAAAATATGCTTATTAAACAC1680.11425850987860033No Hit
TTATCGGGATGCATATTTGCACTTCCAGATGGGTTGCATAAGAATCAGGTCC1680.11425850987860033No Hit
GTTCTCGGGGATTTCATAAACAAGATAGAAAAATCCAGTCATGAAGAAAAAC1660.11289828952290272No Hit
GATCTGGGGATCTCTGATCTTAGATGCCACCTTATACCTTGCTGTACATATA1660.11289828952290272No Hit
GTAGGAGGGGTGAATATTTAGTATCGTGAATATTAAACCTTTGCAGCTGAGT1660.11289828952290272No Hit
ATTGATGGGGCCAAAACTTACCTATAAATTAAGAAGAGTTTTTATTACAAAT1660.11289828952290272No Hit
CTCTAAGGGTTAATACAATCACTATGGAGAACAGTTTGGAAGTTCCTCAAAA1660.11289828952290272No Hit
CTCGCGGGGACGTGGGCCGAGAGCTGACCCGCAAGAAGGGCATCCTCTTGCA1650.1122181793450539No Hit
CACACGGGGGTCTCGTGGTGCGCCGTTTTTTAAGCCGGTCTGAAAAGCGCAA1650.1122181793450539No Hit
ATTTTGGGGAGGGGAGGAACCAGTTGTTCTGTAGCCTAGGAACTGCCTCAGT1640.11153806916720509No Hit
GTTAGTAGGGCTTATATCAAAATCAGCTAGAGTTAGCATCTACATTATTGAA1640.11153806916720509No Hit
GGTATTGGGCAAGCTGTGTCAGAAGCCAAACAAGAAAACGAAAATATGAGAA1630.11085795898935627No Hit
CTAGTTGGGGAGTGACTCAGACTTTGGTCAGAGTCAAGAGGAAGTGACCTTC1630.11085795898935627No Hit
CAATACGGGATGACATTATATCTTAGATGTGTAATTTATGAACTACTACTGG1620.11017784881150747No Hit
TATGAGGGGGTTTTTTGTTTTTGTTTTTTGTTTTTTTAATTTGTAAAATCTG1620.11017784881150747No Hit
AGCGTAGGGCATTAATGACGTAGAAATAAAGTCTTTCCTAGTCAAGCAAATG1610.10949773863365864No Hit
TTCCTGGGGAAGCCTGTGTAAAATGCTACCAAATGGCAAAAAGCAACAATAA1610.10949773863365864No Hit
GTCAACGGGATTTTCCTTACCGAATATAGTGAGGAAGCGTCACAGAATAACA1610.10949773863365864No Hit
GCTGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1600.10881762845580983No Hit
CATAAAGGGTGTCTCAAATACCTGTCATTGGCTTCTCTCCTCTACAGTTTGA1600.10881762845580983No Hit
GCAAATGGGAGGGGAGCCTCTCCACTTTCATTTCAGTCTGACCACTTTTCTT1590.10813751827796103No Hit
GTGCTTGGGTAGACCTAGGCTTTGAAAGGAAAAATAAGTCAGCATCCTTTAC1590.10813751827796103No Hit
CTTGATGGGGATGGAAGAAACCTCACTGATTAATTTTAAGAACCTTTTAGGG1590.10813751827796103No Hit
TGCGATGGGACAATCATGATAGCATGTGTCAGCCCTTCAGACAGAGACTTTA1590.10813751827796103No Hit
CTTTACGCGGGGGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCT1580.10745740810011223No Hit
CAAAAGGGGCGATCTGTGAAAGGGATATCTTTCAGTGTTTCTGCAAACCTGA1560.1060971877444146No Hit
CAACATGGGTAAAAACACTCTCTGTATGCATTGTTTCATGGGAACTCCACCA1560.1060971877444146No Hit
ACTACCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1560.1060971877444146No Hit
GCTACGGGGTGAGGCAGAGCCCCTGAAGTTCTCTTTTTGTCCCAAGGAGCTT1560.1060971877444146No Hit
TGTAAGGGGATAGTGGTTTGCTACTGTGCATATCTTCCCAGCTCCCTTTTCA1550.10541707756656579No Hit
TACGCAGGGGAGTTGAAAAAATTTAAAGCAACTGTAATAATGACAAAAACAT1550.10541707756656579No Hit
CATGAAGGCAGAAGCGGTATTTTTAACAAGCTCCCAGGTAATTCTGATGTGC1550.10541707756656579No Hit
CAATTTGGGGAGCCGGGTTGTATTTGGAAACGCGGAGTGAGTTTTTCCGTGC1550.10541707756656579No Hit
CATCTAGGGGTGTATCAGGGATGAGATTTCTACTGCACACCCAACTTGCAAG1540.10473696738871696No Hit
TCTTATGGGTATTCTAGCCTAGTTGATAATTTTTATTTAAAAGGTGGTCCCT1540.10473696738871696No Hit
AACTTAGGGTGACTTCCAAGGGCCCTTCTAAAATTCTGTGAGTCCATGATTT1540.10473696738871696No Hit
ATTTGGGGGCCGGGCGGCGGGAGCTAGACGAGCACGCAGGGCACCCCTCGGG1530.10405685721086816No Hit
TTTTGAGGGATGGCAGGCCATGTGAATTTCCTTGCTTCGCTTGTTATAAACC1530.10405685721086816No Hit
TACCAAGGGGACCAAGGATGCAGCTTGACTTTAAATCCATTCATGTTCAAGA1530.10405685721086816No Hit
CGGTTTGGGATATGAATGTGATTAAAATGATGACGCAATTGCTGCTGCAACT1520.10337674703301936No Hit
AGACTTGGGATCGAGGCTTCATCTTCAAGCAGACATCGAAGCCGTTCTGATT1520.10337674703301936No Hit
AAGGCTGGAGTGCAGTGGCACAATCTCAGCTTACTGCAACTTGTGCCAACCA1520.10337674703301936No Hit
TGATGTGGGAACAAAAGGATTGGAAAACTCATCTATTTTTTTGTTATTAAAT1520.10337674703301936No Hit
TATGCCGGGATATTGGTTAAGATTTGTTTAGGCTGCTGTGGCTAATAAAAAT1520.10337674703301936No Hit
ATATTTGGGCGAGATGAGCTGCACACAGAGCAGGATGCCATGCTGGTGGACA1510.10269663685517055No Hit
GGCTATGGGTCTGGCTCTGGGATTTCGCAGAAGCTTTTAGCATCGGATTATC1510.10269663685517055No Hit
TCTCGCGGAGGCGAGAAAGGGGACTGGTCATCTATAGAAAAATCTGTGAGAG1510.10269663685517055No Hit
AAGACTGGGGCGAATATGTTGTAGTTTGGCTTCATTCAGTTTATTTCCAAAT1500.10201652667732172No Hit
TATCCTGGGATATAGCATATAGACACTAAGTGAAAAATCTGAAAAGAAAATC1490.10133641649947292No Hit
AAGCATGGGATTGTGCCAAGGACTTGAATGGATGATGTCACGACTTAAGATT1490.10133641649947292No Hit
GCACAGGGGGATCTGCTCACGGGCTGATGCTTCAGTGTTATAAATTCAACAT1490.10133641649947292No Hit
AGACCAGGAAGTTTTCTTACTCATCTCTTCTTACAATGGGTCAGGCACCGGG1490.10133641649947292No Hit
CATTTGGGGGTTTTGTAGATGTTTTCTGTAGGGACCAGCCCCACAGGGTTGG1490.10133641649947292No Hit
TTTAAAGGGCTTTGCACCCTGGGAACCTCTGACCATGTGCCTGCCTGTCCTT1490.10133641649947292No Hit
TTGTTTGGGCAAGATGCTTTGCTGAAGAAAGAAGACCTATTCTCCCTTAATT1480.1006563063216241No Hit
CATACAGGGCAATTCAATTATTTTCTGTTATTAATGTTGCCACTGCTTTCTG1480.1006563063216241No Hit
GAAATCGGGGTTTCTTGAATCTTATTTGTTAATCTGACTCACAGCTGAAAAG1480.1006563063216241No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)