FastQCFastQC Report
Sat 31 Dec 2022
SRR5846950.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846950.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168241
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGC7540.44816661812518943No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTGAGGTATCTCGTATGCCGTCT6810.4047764813571008TruSeq Adapter, Index 2 (95% over 21bp)
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG3110.18485387034076117No Hit
GCGTTAGGGGCGATGGAGGAAAATTTACCAAGCAAATGGAAAGCAGAAAAAA2090.12422655595247295No Hit
ATTGTTGGGAAATATCTGAACTGACAAAATTTTAATTTAAGTTTTGTACCTT2070.12303778508211435No Hit
GAAGTTGGGTCTGCATCGGGGGTCCGGGTCGCCGCCGGCAGCAGCCTCCCGG2050.12184901421175576No Hit
AACGAGGGGTCATTTTACAACTTCCTTTATCCCTTTTTTCCAATTTTGTCAG2000.11887708703585927No Hit
GTATCTGGGATGTGAAAAAGCACAAGAACAGAAAATAAAATACCTTAAAGTC1990.11828270160067998No Hit
CGCCTGGGGGAGATGAACAGCATCGAGCCTGCTAAGGAGACCACCACCAATG1900.11293323268406631No Hit
GTAACAGGGATCAACAGGCTAAATATTCAAAATCATACATTACAATCAATTA1870.11115007637852842No Hit
GCTTTAGGGCAGAAAACAAAAGTGGAGTTAATAACCGTTTTTAAAATACTAC1860.11055569094334913No Hit
AGGCTCGGGATTTACCTTTGCTTGGAAACCTCTCCAGCTAAATATCCAATTT1840.10936692007299054No Hit
TCGTTTGGGGGTCCCAATGGACCTTATTTCTGTTCTACAATACATTAAGTTT1810.10758376376745266No Hit
TGCTCAGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGT1800.10698937833227334No Hit
CTTAGGGGGGGTGTGGGTCGAGCAGCGGCGGCGGCGGCGGCAGTGGCGGTCC1800.10698937833227334No Hit
AGACCTGGGCTGTAATGGTCTCTAACATGAATAACAAGATTAGAGGGACAAT1790.10639499289709406No Hit
GTTTAAGGGATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACAC1760.10461183659155616No Hit
GCGCTTGGGAGGGAGCATGTAGCTAAAATGAAGAAGATGGAGATGGAGATGG1760.10461183659155616No Hit
GGAAAAGGGAAGAGTATTTTTCTTAAAAGTCTAGATTTTCTTTTGTTTAGTC1760.10461183659155616No Hit
ATCAGTGGGGGAAAATGCCAGAGGGGTGGTCAGAGGAGGATGCTGGAAACGG1760.10461183659155616No Hit
TTTAGAGGGGCAGGCCTTGTGATAACTAAGCGCTACTTTTGACGAGCCTTCA1740.10342306572119757No Hit
TTTCGTGGGCATTAGAATTGAAAGAAAAAAAGTATATAAAGTGCTTAATACT1740.10342306572119757No Hit
CGCGCTGGGAGGTGAAAATTTAACAGTTTCTAAGCTATTAAATTCCTATGTA1730.10282868028601827No Hit
ATATTCCGGGGTTTATCTAACTTTACAATAAAGCTTTTTTTCCCCTATAAAT1730.10282868028601827No Hit
TCTAGCGGGAGATCAATAGGAAATGTGCTTTTTGAGGAAATTTTATTTTAGT1720.10223429485083897No Hit
CAACTAGGGGAGTCTTACCTCGTTGTGGCGTCAGAACCGCCGTCGCTCGCTC1720.10223429485083897No Hit
CGTTGCGGGGATGTAAGGATTGTTCAGCAGTTCGGCATTTGGGATCGAAAGC1710.10163990941565969No Hit
CAGTTTGGGCAACTTAAGTAAATGGAACTTAGTGATAGATATGTTTTGTTTG1700.10104552398048038No Hit
CAGCGTGGGTCTACGGCATGGGGAGAATAATTCTTGGTCTTTCAAATAAAAA1700.10104552398048038No Hit
AAATACGGGTTGTTGCGAAGGTTTCAAATATTTGTGACTGAATGTACGGTGA1690.10045113854530109No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)