FastQCFastQC Report
Sat 31 Dec 2022
SRR5846774.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846774.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126584
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGCCGTCT5240.413954370220565TruSeq Adapter, Index 7 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGC4980.39341464956076594No Hit
AATCTCGGGATGGATCAGTGGTTTCTAACGTTGGTTTTGTGAAATGTTATGA2020.15957782974151552No Hit
TTAGCAGGGGTACTGCTAGAACTAATAAATAAATTCAGTATGATTAACAGGA1950.1540479049484927No Hit
GCGGTTGGGCAGTAATGATATTGTGAGTTAGGATAATAACTTTTTTTTTTTT1920.15167793718005435No Hit
ATGTGAGGGGGTATTAAAAGTGAGTTGAGGACCTAGTGCTATTCATTCAAAA1840.14535802313088542No Hit
ATATGCGGGCATGCCGGGAGTGATCAGTCGGACATTTTAATTTGAACCACGT1790.14140807685015483No Hit
ACAGTCGGGATAGTTTGTGAACCCTTGTTCCAGTTGATTTTTTTCCTAGAAA1710.1350881628009859No Hit
CATTGTGGGCTACAATTCTGAATTTGCTTTTCTTGCCTCTCTTTAGTCACCT1700.1342981735448398No Hit
TGGTTGGGGGAAGAGAGGTTTGAGAAAAGCACTAAGGAATTGATGGCAATAA1700.1342981735448398No Hit
TAACGAGGGAATACCCAACTGTCAATATCAGACAGATCGAGACAGAAAATTA1700.1342981735448398No Hit
CGGTCCGGGGGCACTGGCCTTCAACCAAGCCAAGCGCCTTGGCTTCTGTCCT1690.13350818428869368No Hit
GCTAATGGGGGATGAACATTCCCTCAGGGATTAGGGATAAGAGAAGTTAGCT1690.13350818428869368No Hit
GATATAGGGCAAAGGTATTGAATCAATAAATGAGAAACAAAGGCTTCTCTGG1670.13192820577640144No Hit
TCACATGGGCTGGTCCTGGGTAAATAAAAACATGTTGCTTTGTATACATTTT1670.13192820577640144No Hit
GACAATGGATCTCACGTGAAGTGAGCTTCGTTTGTGCTCATTAAAAGTGAGC1650.13034822726410922No Hit
ATAAGGGGACCAACGATGTAAAAGCATTCCTATTTCTCCACAGCCTCGCTAG1650.13034822726410922No Hit
TAGCAGGGGCGGCTTGAGAAGACTGTCAGGAAACGAGTATGTGTTTACTAAG1640.1295582380079631No Hit
AATACTGGGAGGTTTTTTTGTCTTTCCTTGCTTGTGTTTTTGGTACTATAGC1630.12876824875181697No Hit
TAACTAGGGAAAGTGACGGTCAGCAGGGGGTTCTGAGGCCCCACAGCGCTGG1590.1256082917272325No Hit
GACGATGGGCATCCTTGTCTCTACCAAAAAATTTAAAAATTGGCCGGGCGTG1590.1256082917272325No Hit
CAGTGAAGGGCTGACCTACAGACTCCATCAGCATCCCAAGTGATTCTCACAC1580.1248183024710864No Hit
AACTCAGGGCTCACTGCATCACATAGAATATTATAAAGTTATGGACTCAAAG1580.1248183024710864No Hit
GGCGCAGGGGATTGGCTGGTTGCCAAGAAATATATATTTGTCACTAGGGCAT1550.12244833470264804No Hit
GCACAAGGGGACATAGAATGGCTGAATGCATAAAAAGCAAGACTCATTTATC1550.12244833470264804No Hit
ATACCCGGGCAACAGAGTGAGAACCCGACTCATTTAAAAAAGGGGCAAAGGC1520.12007836693420969No Hit
CAATTAGGGTGCCTTATGGTAAAATTTACTTAAAACGTTAAACTAAATGAGA1510.11928837767806358No Hit
TTCGCTGGGTCTTAGAGGAATACAATAAATGCCTAAATGAATGATGCACATT1490.11770839916577135No Hit
TAAAAGGGGGTTAATAACTGATTAGGTTATCATGGTGAGTAGCCCTGAGATA1490.11770839916577135No Hit
TCAATCGAGGACTCCCTGCTTTGTAGCCGGAGTTTAGGCCGGAGCTTAGAAA1480.11691840990962522No Hit
TTGCCTGGGTGCCAGTTTGTATTATAATTGTCTGAACTCATTGACCTACTAT1470.1161284206534791No Hit
TTGAGCGGGGGGCCGCCGGAGCGCTTTGGAAGGCGCACGGGGCGAAGATGGC1470.1161284206534791No Hit
TAAAATGGGTCTGTATTTGAAATGTTGAGTACGGTTTCTGGTAAGTCACATT1470.1161284206534791No Hit
CTGCATGGGCCAGCCTGGGCAACAGAGTAAGACTCTGTCTCAAAAATGAATG1460.11533843139733299No Hit
TAGCAAGGGCTGTGGGTGATTCGCCTTGAGAGATTACAAAACAAAACATTCT1440.11375845288504076No Hit
AGCTGAGGGAGGACAGGCAGAAAGGCGATAATTTGTATCTTTTAATATAAAA1440.11375845288504076No Hit
TACACTTGGGATTCAGACTTTATAGTTGTTACCTGCATGACTGTCAGTCTAG1440.11375845288504076No Hit
ATATCAGGGCAACGGGGGCAGAAAAGATGATTTAGACTATGGAAACAAAAGT1430.11296846362889464No Hit
CGTACTGGGACACAATAAAAAACCAAAACCTGGAAAAGAAATACTTAAAAGA1430.11296846362889464No Hit
TATGCTGGGCTCACATCTGCAAAGGGATTTTGCAGATGTGATCAAAGTAAGG1430.11296846362889464No Hit
TTTACAGGGGGGAAGCATTTTTACCTTATTGTAGGGCAGTTTTGTTTTTCAA1430.11296846362889464No Hit
AGGACTGGGACTTCCCTCTGGCCACATATGCCCCTGTCATCTCTGCTGAGAA1430.11296846362889464No Hit
CAACGTGGGAGAGTAAAAGGGGAAATGCCAAGATTTTGGGGTTTTTTTGTTT1430.11296846362889464No Hit
TAAGTTGGGGAGAGGGGATGTAGGCCCATGGGCACCTGGCCTCCAGGAGTCG1420.11217847437274853No Hit
AGTATAGGGTAACTACCAAGGAAAAGAAAATTGGAAACAATTTAACTAAATT1420.11217847437274853No Hit
TTAGCTGGGTGCACTTATTTTCCAGAAATAAAAATATGAAGAGCTCACGCAG1420.11217847437274853No Hit
TTGCTAGGGGGCTGCCCGAGGGTTCCTCGCAGGCTGGAGCTGACGGCGCTGG1420.11217847437274853No Hit
GAATACGGGGGATAGTCAATTCAATTTTATTAGTTTTAGAGTTATATGAATT1410.1113884851166024No Hit
CAGCAAGGGATCTTAACGCGGGCTATAAATAAAACATCTTTATCTTATTTGA1410.1113884851166024No Hit
CACACGGGACCAAAGGAATTTGCATCCAGCAGCAGCACTTAGACCTGCCAGC1400.11059849586045628No Hit
GTGATGGGGATTACTTGAGGACTGTGTCTGCTCCGTGAGAGCTGCGTGGGCA1400.11059849586045628No Hit
AGAATAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1400.11059849586045628No Hit
TCATATGGGGCTTATCAGTCCACCAAGAATTCAGCCCATTTTCTCTCTCTTG1390.10980850660431017No Hit
TTAAGCGGGTGCTGAATTGGGCCTCCTGCTTGGTAACCAAGTAAATGAGCTG1390.10980850660431017No Hit
TGTAATGGGGGCTTTTGACAAGATGGCGGCAGGAGGCAGTGGCGTTGGTGGG1390.10980850660431017No Hit
CGACCCGGGAATTCACTACATTAATAAACTGAAAAAAATCACATGATTATCT1380.10901851734816408No Hit
TCAATTGGGCTGTGGGCATGGGGTAGAGGGGACAGAATGTAGGTAACAGAAA1380.10901851734816408No Hit
GTTTCTGGGGAGGAAGGATTTGCTCACAATTTTGTAATTGAATTCATTAAAA1380.10901851734816408No Hit
GAGTACGGGTCTGCGAGGGGGACATTAAGCAGCTGTGGCATCTTAGGAATCC1380.10901851734816408No Hit
TACGCCGGGCTATATCTGAAATTGTATTATCTATTTTTATTTCACCTTTCTT1380.10901851734816408No Hit
GTCAAAGGGGAGGACACGGGTCGCTGGCGGGTTAGGTGGCTGCGTGGCCGCC1380.10901851734816408No Hit
TACCTGGGGGAAGCACAAGCAGAGCGGCGGGAGCGTGGGTGCGCTGGAGGAG1380.10901851734816408No Hit
GCTAACGGGATACTTTGCTTGGTCCTATAGGCTCTCAGGTTTTTTTGATATC1370.10822852809201795No Hit
CATGCGGTATGTCATGATATCATAAAAGGGACACTGGGAAATCATTTGATTC1360.10743853883587184No Hit
ATAGCTGGGATGTGGTCATGGTGACTTTCTGATAGGTAGTGGCTGTTGCAGA1360.10743853883587184No Hit
GGAATTGGGGTGTAGAAGGGACACTTGCCAAGCACATCATGTCTGAACCTGA1360.10743853883587184No Hit
GTTTGCGGGTAATAACCAGGATCTATAAGTAATTTAAACAATTAAGCAAACA1360.10743853883587184No Hit
CGCCCAGGTGAATCTCAATCCTTCCCCCTGAATAGCACGTTTCAACTAGGAA1360.10743853883587184No Hit
ACAGCCGGGAAGGGAGGAGGTGTTCTTGAAGACAGGAGGAGGCTCCCGCTGT1360.10743853883587184No Hit
CCATGAGGGAACTGATTCCATGCACTAGTACATGTAGGCTTCTCCCTTGTGC1360.10743853883587184No Hit
GTCTAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1350.10664854957972572No Hit
TTCGCAGGGGAGGGCGGCTGCTGCTGCTGGGACGTGCGGCTGGACCAGCCCC1350.10664854957972572No Hit
GGTATAGGGGTGCTGCCCCGGCGGACTTTGAGCCTGCAGCCTGGCGGTGGGG1350.10664854957972572No Hit
CGCCCTGGGTGGAATTAGTGGCTTGCTGATAATCTCATTTTATAATTTGTTC1350.10664854957972572No Hit
ATGTATGGGAAGACCAACATCGTCACAGCTTCCGTGGACGCCATTAATTTTC1350.10664854957972572No Hit
AAACGTGGGGATATAGGATTCAGTTCCACAAGAAGACTTAACTATCCTAAAT1350.10664854957972572No Hit
TGTGCTGGGGATTTTTGTGCGTTGATTTTGTATCCTGAAACTTTCCTGAACT1340.10585856032357961No Hit
TGAACTGGGATACTAAAATCATAGTTTGAATTGCTGACATATTAATTGTGGA1340.10585856032357961No Hit
GACAAAGGGGTCCAAAAAAAGAGATTTTCATGTCAATTACTGTGTTTCTCCT1340.10585856032357961No Hit
TTACTGGGGAGCCTCAGGCTGCACTGTGAATGAGCCACTTGTGTTTCCACCC1340.10585856032357961No Hit
CTGCAAGGGTTCCAGGTCAGAAGAAATTGTTTCTTAGACCTCTCAGACTAAC1340.10585856032357961No Hit
AAAGCCGGGGTAAAAAGTCAAAGCCTCAAAGAAATGGCACAACCATGTTCTT1340.10585856032357961No Hit
ACAAACGGGGGAAAGGATTTGATGAAATGTTCAAGTCAAATGCAGAATAAAG1340.10585856032357961No Hit
TCAAATGGGGATTGCCCAACTGTAAAATATCATGGCTTGAAAAATGCATCCA1340.10585856032357961No Hit
CAGCCGGCGATCGGAGTGGGCCTTTTCTTTCTTTTTGTTCATTCTTTACCTT1330.10506857106743349No Hit
GCAATAGGGGATGTAGGTGTGTGTTTACATGGCTGTTTATTTTTAAGAAATG1330.10506857106743349No Hit
GCTGCTGGGATCCGAGGTGCTTGGGAGTTTTGAATGTTAAGAATTGACCATC1320.10427858181128738No Hit
AAAACTGGGAAGTCTGATAACACTGTGATTACATATGTATCAATATAGTATG1320.10427858181128738No Hit
TCGCATGAAAAAGGAAACAGAATAGTCAATGGACAAGGAGAAGGGCCTCCTG1320.10427858181128738No Hit
CACATGGGAATCGTCTTATCACTTTTTGTAGGCCATTAATGGTCAGGAAATT1310.10348859255514126No Hit
TCACACGGGGAACCATCAGTCTGCAGTAATCCAGTCAATTAAAAATGGATTT1310.10348859255514126No Hit
TATCGGGGGTCTTCGTCTTAGTCCAGCAAGCACCGCCAATCCTTTTCTTCCC1310.10348859255514126No Hit
AATACAGGGATCGACTCGGACACAGAGGACAGCGGCAGCGACGAGAACCTGG1310.10348859255514126No Hit
ATCAGCGGGACCAAAGACTTTACCTTTTTGGAATATGCTACCTCTATTTCAA1310.10348859255514126No Hit
TGGTTCGGGTAACTGAGGAAGGAACAGAGCTGTCACAACGACTTGTGAGGAG1310.10348859255514126No Hit
GTTGCCGGGCAAAATAAAACGTGATGAACGCACCTTAATAAATCCAGATATT1310.10348859255514126No Hit
GTATTAGGGAGTGGAGATTTGGTTTATGTTACCATTAAGTTCCTCTAAAAAG1300.10269860329899513No Hit
TTCATAGGGTGCTTAAAGAAAGGCAGGTGCATTGCTCTGAAGTGCATATAGC1300.10269860329899513No Hit
TACCTAGGGGTGACTTTTTGCTATCTATTTTTCTAAAATTTTACATTTCAAA1300.10269860329899513No Hit
CTTTTGGGGCAATGAGACAGAAAATTAACAAGGATATTCAGGACTTGAACTC1300.10269860329899513No Hit
AAAGTAGGGAATTAGCATATAGAAAAATATTACTTTAAGATGACTTGTTTCC1300.10269860329899513No Hit
GTACATGGGATTCATTATCTCTGTAGGAACTTTTAGTTCCATTATATGAACA1290.10190861404284902No Hit
TGTCTGTGGGTAGAATAGTATACCCAGTGAAAATATCTTTCAAACAGGAAGG1290.10190861404284902No Hit
ACAGCAGGGCGATCTACTCAAGGGCAACCAGATCTCTCAGGAACTCATTGTT1290.10190861404284902No Hit
GAAAATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1290.10190861404284902No Hit
GGATTGGGGAAAAATCAAGAATTGTACACTGTTTAATGAGTTCTCCATATTC1290.10190861404284902No Hit
GCAGCTGGGTACTAGTATTAGTTTTATTAATGCCCTATAAACCCAGCAAGCA1280.1011186247867029No Hit
ATTCTAGGGAGCTTTTTTTATTGAGTATAGTTTCTTTTAAAACATGGAATGC1280.1011186247867029No Hit
GCAATTGGGTTAATTAGGTAAACTTGAATATTCCCAGTAAATGTTTGAGAAT1280.1011186247867029No Hit
GATGTTGGGAATTTGCAAATAGTCATAAGCAGATTATCTTCTTTTTAGCAGT1280.1011186247867029No Hit
CGAGTAGGGAGGCCATGCAAATGAAAATTTTTCCTTCGTCTTACAGATAACT1280.1011186247867029No Hit
CTTGCTGGGAACTCAGGGCTTCCATTTCCTCAACTGTAAAATTGGGAAAATA1280.1011186247867029No Hit
CACAAGGGCATTATATTGAGTGAAAACTCCAATCTTGAAAGGTCACATCTGG1270.10032863553055679No Hit
ATCGTTGGGGCTTCCTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAA1270.10032863553055679No Hit
AAACAAGGGCTCCATTTCTTTATGCCTGGCTTTGCCCCTCTCACCAGCCGTG1270.10032863553055679No Hit
GGTGCTGGGCATTTATATAATGCATTACTGGACTCAAACGCAGCCTTTCAGT1270.10032863553055679No Hit
TTTCTTGGGAGTGAAAGCAGTGATTAGATACGATTTTATATCCATAAACACC1270.10032863553055679No Hit
ATGACAGGGCTAATGTGGAAAATTAATTACCTTTATGAAGGTGGTTTATTAC1270.10032863553055679No Hit
CTAGTAGGGTGCATGTAGAAGCTAAAAATATACCCAATTATAATGCATCTTG1270.10032863553055679No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)