FastQCFastQC Report
Sat 31 Dec 2022
SRR5846757.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846757.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164809
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGCCGTCT7600.4611398649345606TruSeq Adapter, Index 7 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGC7210.4374761087076556No Hit
GAGTCATCTGACACTGATGATGAAGAATTACGGCATCGAATCCGGCAAAAAC2240.13591490755966No Hit
ATCATGGGGGTCTCGGTCCAGGCCTTCTGCGAAACCGTGGAGACCGCCGCCG2170.13166756669842059No Hit
ATAAGCGGGATCTTGGCATAACATGGTTCTTTTTTAAATAGAAACTTTAGTT2160.13106080371824355No Hit
TACACGCGGGAAGTGCAAGCATTTTTAACTAGATGGAGACAGAAAGCACATG2010.12195935901558774No Hit
GGAAATTTTACTTGGGGACCAGGAGGATTTGAAAGGCTGCATGTACTCAGAA1990.12074583305523363No Hit
GGTTATGGGCTGCTGATATAAACTTACATAACCATTAATATTCAATCCTCTT1950.11831878113452543No Hit
GGCAAAGGGAGCTGCAGCTAAGGAAGCATGACCACAAAACCAATAAAAAAGA1940.11771201815434837No Hit
TAACGGGAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGT1910.11589172921381721No Hit
CACTCGGGGAAATAACTATAGCTACAACAATTTATTAATGGATATACAATAT1870.113464677293109No Hit
GACAGTGGGCTCTTAAATCCAGAAAGTTATATTTTGCCGGAAGGAAGAAGAA1860.11285791431293193No Hit
GGTTGCGGGAACACAGGGTGTTTAGCAAACATTTAGTTATTTCCTTAAGCCA1860.11285791431293193No Hit
GATAATGGGAAGTTACTTAAAATCTCTAAGTCTCAGTTCTGTCATCTAGAAA1850.1122511513327549No Hit
AACGCGTGGGAGATTAAACAAAAAATACTATAAAACCCCTGACCACATTTAA1850.1122511513327549No Hit
TAGTAGGGGCTGACAGTTCACCAAATAGCTAGTCATGGAGAATGCAGGCAGT1830.11103762537240079No Hit
TTCCTGGGGCCCGACATCCATTCTCTGGTACAGTCAGACCAAGAAGCTCAGG1790.10861057345169256No Hit
CTTCACGGGCGAGAGCGTGTCCTGGCCCAGCTTCTTGGCATGGTCTGCGACG1780.10800381047151551No Hit
TGCTGTGGGATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAA1770.10739704749133845No Hit
CTTCACGGGAGATAAAATGAGGGTTATCAGCTAACTGATATGCTATCATTGA1770.10739704749133845No Hit
CACCGGGGGGTCACTAAATGGTGAGATCATCAAGCCATTTTAAGTTCTTTCT1760.1067902845111614No Hit
CTTCTAGGGCATAGACCTAATTTGCAAACTCAATCGGGGACTAAAATTTCCC1730.10496999557063023No Hit
CTCGTTGGGGCAAGGCTACAGTAGCAAAAACACCATGTTTCTGGTACAAAAA1710.10375646961027613No Hit
TCAATGGGGGGCACTCGCGCAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGT1710.10375646961027613No Hit
AGCATTGGGAATGGTAGGCACTGTGATCATGATGAAAATGAATGAGGATGGC1700.10314970663009909No Hit
GCACTGCGGGATCTTGGAATTCAGTAAGTGCATATCCTAACTTGCCCATATT1700.10314970663009909No Hit
AGACCAGGGATCTAGTCATGGGCGATCTTTTGATGGGAGGCTTTGTCTGGAA1690.10254294364992203No Hit
ATATGGGAGATTTAAGTGTAAAGTGATTTGAAATCTGAAATCTTTGTAAGAT1690.10254294364992203No Hit
AAAAGGGGGTGACAGAGAAAGGAGGCAAACCAGCTTGTCTACTTCTTAAACT1690.10254294364992203No Hit
GTCCTAGGGGGACTGGAAGAACTCCAATCTTGACAGACACAATCTCCAAGAC1680.10193618066974498No Hit
TGCGCGGGGAGTATCTGCCATGGAAATATTTCTGTTTTTAGATATTTTTGTG1680.10193618066974498No Hit
TTCGCGGGAGCGTCTAATGAGCTAATTTTTGTCCAGTGATCACATCTTGAGA1670.10132941768956792No Hit
TTTATTGGGTATGACTGAGATTTATTTCTCTCTTTTAGATGAAATTGTTGAT1670.10132941768956792No Hit
CAAGATGGGGGACGCTCTTCCGACCTACACCAGGGTCTCACCCTGGGTCTTA1660.10072265470939087No Hit
TTAAAGGGGCGATTGATATTTATCTGATGGGTTGCTTTGTGTTTGTGTTCCT1650.10011589172921381No Hit
AGGCAAGGGCATTGATTACTAAATCAGAATTCATTTAGCTCACCACATCTCT1650.10011589172921381No Hit
CGCACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1650.10011589172921381No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)