FastQCFastQC Report
Sat 31 Dec 2022
SRR5846730.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846730.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172447
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGC14160.821121851931318No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGCCGTCT13580.7874883297476906TruSeq Adapter, Index 7 (95% over 21bp)
TTGACTGGGGCGCATGATGAAACTTCGGCTCACTCCTTGGCGCCTGCCTGAT2370.13743353030206382No Hit
CAAGCCGGGGCGGCCGGATGGGGAAGGCGGCGGCGGCGGTGGCCTTTGGGGC2370.13743353030206382No Hit
TAGCTGGGCAAGCCAAAGGACCATCTCTTCTTTCTTCCACCTCCCTTCACCC2200.12757542897237992No Hit
TATGCTGGGGTAAGGGGCCAATTCTAAGTATTCTTCACGGCAGCCAGAAGTT2190.1269955406588691No Hit
TGAAGAGGGAGACTACTTCCTGTAATGAAATTTTAAACTTGTACAGTATTGC2150.12467598740482583No Hit
ATTATGGGGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTCAGAGGT2150.12467598740482583No Hit
GCAAAGGCTGACATTTATTTGTTAGAAAATTACACACATTTAAAAATTCAAA2120.12293632246429338No Hit
GACGAAGGGTAAGTGGTAAATGAAAGTGGACTTTGATTATTTCTACTATAAA2090.12119665752376091No Hit
GTTTCGGGGCTCTAATGGAATGGAATAGAATAGAATGGACTCAAATGAAATG2080.12061676921025011No Hit
TTGCCAGGGATGGGAATTCAAGATGAGATTTGGGAACAGACACAACCAAACC2080.12061676921025011No Hit
AATAGAGGGATTTCTCAGCCTCATGTTCTATTTTAGCTCACACACGACCCAT2040.11829721595620685No Hit
TGTGACGGGGGGGAGCTCTTTGAAGACATTGTGGCGAGAGAGTACTACAGCG2030.11771732764269602No Hit
AGCCTTGGGAGCGAAACAGATAGATAAATAAGAGATACAATCTAGGTAACTT2020.1171374393291852No Hit
ACTAAGGGGCATCGTGGGCATAAAATGTTCACCTTTTTTCCAGATGAGTACA2010.11655755101567437No Hit
CTTCGCGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA2010.11655755101567437No Hit
TTAGCTGGGGTCATCAGTTAAGGCTATTTTTACTTCTTTTGTGGATCTTCAG2000.11597766270216357No Hit
GCATCCGGGGGATGAAAAGGCAAGCTACAGACAGAAAGTATTTGTAAACCAG2000.11597766270216357No Hit
AAACTAGGGGGGAATAGCATTGAATCTATAAATTACTTTAGGCAGTATGGCC1990.11539777438865276No Hit
ACTTCAGGGGCACAGAACTGCAGTCTTGTTCTTCCCTTGGATCATGACAAAT1980.11481788607514193No Hit
GAGCTGGGTACTGACATCAGTTTGGGTGAGAGAGGGAGTCACTTTACTGAAA1980.11481788607514193No Hit
CAAATGGGGATATAAAAGATGGTAAGAACTGATTTCCAGAAGGACTTCAAAG1970.1142379977616311No Hit
GAACATGGGATAGCATCTTTGGTAGATGGAGAGAGATCTTTTATGACAAAGA1960.11365810944812028No Hit
GTCGCGGGGTAACAGTAATGGGGTTTGAGAATGTTATTACTTTTTGATTATT1960.11365810944812028No Hit
GCTCCCGGGAAGAGACCTGATGCGGGACCACAAGGCCTGTCTGCCTGATGCC1960.11365810944812028No Hit
CAGTCTGGGAGGGCCGGTGCTGCCTGCGGAAGCCGGCGGCTGAGAGGCAGCG1950.11307822113460948No Hit
AAGCGAGGGGTTCCCCATCTGGTACTAATGACCTACTAGCCCTGGTTATTTT1940.11249833282109865No Hit
AGTCCAGGGGGGGCACGCTGGGACGTCTCGCTGGCGGGAGGCCACGGGCTTT1940.11249833282109865No Hit
TTCAAGGGGATAAACTTTCTACTTAAAAATTTTACTTTTACCCAATAAACTT1920.11133855619407701No Hit
CGTCGCGGGAGTAAAAAGGGAATAAAACTAGATGAGTGGTTTTCAAACTTTA1920.11133855619407701No Hit
CTTAATGGGGCTCTCGAGTCACTCCGGCGCAGTGTTGGGACTGTCTGGGTAT1920.11133855619407701No Hit
TGGCATAGGGAACTGAAAGTTTGTACCCTTTCACCAACTTCTTCCCATTTTC1910.11075866788056621No Hit
GTTAAAGGGGGAAAACTAAGCATTGGCCTCATGTTCAGTCTTCAGGATATCA1910.11075866788056621No Hit
AACATAGGGATAGAAAAGGAAGCTCCAAAAGAGATAAACATAGATTTTCAAA1910.11075866788056621No Hit
GCTAAAGGGCTCTTCCGATCTCTTGATGGGTATTCCTAATATGCCCACTTGG1890.10959889125354456No Hit
TACATTGGGATTCAGTGAAGATGCCCTTATTTAGATATGTTGATTTCCTTAT1890.10959889125354456No Hit
CAGCATGGGAGAGGGTGGCAGAAGTGCTCAATGACCCTGAGAACATGGAGAA1880.10901900294003374No Hit
GAACCATGGGCTCATTAGGGGCCTTGAAGTTGATAGTATGCTGGTATTCTAC1880.10901900294003374No Hit
GAAATGGGCACAGGATGTTGCTTACCAGGACGGAGTTTTGGTATCTTAGTAC1870.10843911462652293No Hit
AATGTGGGGATATGATTTTTAAATAAATTGTACAGTAATTTTTCTGTTGAAA1870.10843911462652293No Hit
GCGCGAGGGCCAGTATGACTACATCTTGCCTCAAGTTTCTTTCACCGCAGTG1840.10669944968599047No Hit
GAACCAGGGAACTTTGGTCAGGGATTTATTTCCTTTGGAATCTTTGGATTAG1840.10669944968599047No Hit
CAGCCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1840.10669944968599047No Hit
TGCTGAGGGATCAGAACTTGGTCCAATAGTCCAATTTGTTAAAGCCAAGGGC1830.10611956137247967No Hit
ACAACTGGGGGCTGAGAACGCGGGTCCACGCGTGTGATCGTCCGTGCGTCTA1830.10611956137247967No Hit
AGTATTGGGTGGAAGCCTCCTCTTGCTGAAGGAAAATTCCCAGTGCTGCCTT1820.10553967305896884No Hit
TCTGGTGGGGAAAAAGAAAAGTAAAAAAACTTCCCAACTCACTTTGTGTTAT1820.10553967305896884No Hit
AAGCGCGGGCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTC1820.10553967305896884No Hit
TAGCAGGGGGATTGACTACAGTGTTGTACAGAATTTATCATGGATTATTGAC1820.10553967305896884No Hit
GAATATGGGATTGACATTGGTGTTGCAGGGTTCAGACTTGATGCTTCCAAGC1810.10495978474545803No Hit
AGATACGGGATGACCCCAATATGAGATTTTTTTTCTGTGACTCTTCCATGTG1810.10495978474545803No Hit
TCGGAGGGCAGACAGTGAAGAAGAAATTAGAGAAGCATTCCGTGTGTTTGAT1800.1043798964319472No Hit
GTTTCAGGGACTCAATTTTTAATAGTTTTTTTAATGTGTTGGCTGACAGTCT1800.1043798964319472No Hit
TTTTCTGGGGGAGACTATCAGGATCCGGAGACGGAAATGTCCGAAGGCCGCA1800.1043798964319472No Hit
AGAGGCGGGAAGTGATCCACATGCCTTGGTTGACCAAATTGCTGGGATTACA1800.1043798964319472No Hit
ATTTGCGGGACAAGAGTTTATGAATTATACATATAAAATGGCTTGCCTTAGG1790.1038000081184364No Hit
TTCTGCGGGAACCACAGGTCTACAAGAGCGTTTTTAGTAAAGTGCCTGTGTT1790.1038000081184364No Hit
CTTTGCGGGCAAATATAACATCTTAGTCAAAGCCTTGGTAATATAACCAATG1790.1038000081184364No Hit
GTTTGCGGGAAGTTTCAGGCGCACAAACCCGCAAAGAGTAAGACGGCAGCGG1770.10264023149141475No Hit
AGTATAGGGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCGCGAGGCGGAGC1770.10264023149141475No Hit
TTGCCGGGATGAGCTACGCTGGGCTTTGAGGTAGAGGTTGGGGTTTATGAAC1770.10264023149141475No Hit
ATTGCCGGGCAATAGTCCTATTATAGCTTGAAGTATTATGATACATTTTATC1760.10206034317790393No Hit
GTTAGTGGGAAATGCCTGTATTAAGGAAGAAATAAAGCAATAACCTTTCTTC1760.10206034317790393No Hit
TTACAAGGGGAGAATGTCAGTCATGAGCTTAGTGTTTAAGTAACACATCCCA1760.10206034317790393No Hit
AGGCAGGGGATTAAAGTCATATTGGGAAAGATGGCAAAGCTTTTGAGAATAT1760.10206034317790393No Hit
AGCACCGGTCATCAAGTGATATTAAGGTTGTCATCTTAAGAAAAAATATCCA1750.10148045486439311No Hit
TATTAAGGGATCCCAGAATACCACTACTATGTCAATTTAATCTAATTTAATC1750.10148045486439311No Hit
AACGGAGGGGATAAGCCCAATCTTGTTCAGCACGTTCTTCTAAATGCCTGAC1740.10090056655088231No Hit
CGCTATGGGACATCACGAGAAACTTTTGCAAGTGACACGAATGAGGCCTTGG1740.10090056655088231No Hit
TGCCGCGGGGACTTCTAGGAGCTTTGTTCATACTGATTACTTAATCCAATTG1740.10090056655088231No Hit
ATGCTGGGGGTAGTGTCAGAGACAGCAATGGCAAAAGCTGTAAGGCCTTTTG1730.10032067823737148No Hit
TACGCGTGGGATGCACATTCATCCTCACATGTGGCACGTAAGGTCTCATTTG1730.10032067823737148No Hit
GTGGCGGGCTTCCCTATAACTAGATAGCTGCTTGTATTACTGGGACTTTTTG1730.10032067823737148No Hit
CAATGAGGGCTCAGTTTGTGTACAGGAGTGTGCTTTGTGCAACCTCCTGAGA1730.10032067823737148No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)