FastQCFastQC Report
Sat 31 Dec 2022
SRR5846660.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846660.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52240
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCGTATGC31235.978177641653905No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCGTATGCCGTCT21454.106049004594181No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCGTATGCCTGTC8731.6711332312404288No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTA6471.2385145482388975No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAA5170.9896630934150077No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAAT2340.44793261868300155No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAAACTCGTATGC2150.41156202143950993No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCGT1640.31393568147013784No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGG1490.2852220520673813No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTC1260.24119448698315468No Hit
TTGAACGGGCTCTGCATCTGTTCTCTGAGGAACTCAAGTTTGGTTGCCAGAA1180.22588055130168452No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCGTATGCC1170.22396630934150077No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCCTGTCTCTTATAC1070.2048238897396631No Hit
GTTGCTGGGATGAAGTCATGAAGACCACCAAGATCATCATTGGCTGCTTTGT1020.19525267993874426No Hit
GGACCGGGGGAGAAGCAACTTCTAATACAAAGATGACTTATAACACATGAAA960.18376722817764166No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACC940.17993874425727413No Hit
CTCGCGGGGCTGTGGGAAGAACATAGCTGGAGCACAAGCATCAACAGCCAGC930.17802450229709035No Hit
TGCCGTGGGGGCAAGTTGCTTTGGCAGATATCATTCTCATTAATAAAACAGA900.17228177641653905No Hit
CACAGGGGATTCCCCCGCAGCCAACGGGCCGCCCTTCCCCCGCGCCCCCTGC900.17228177641653905No Hit
AAAAATGGGACCACCTTGAAGGGCCTTTTGCTTTCTGTGTTACCGTTTGCTG890.17036753445635527No Hit
TTTCGGGCATTAATATAATAGCTTTGCTTGCAGCCTTCCAATCTATGTTGGT890.17036753445635527No Hit
GGCTTGGGGCAATATCATGTGTAGATATTATGTATAGAAAATAAAATAAATT860.164624808575804No Hit
GTCGCAGGGGTATTTGAATTTAGAGAAACAACATGGTTCCAAGAAGGAGGGT850.16271056661562022No Hit
ACGTGCGGGACCGACTAATCACCACCCAACAATGACTAATCAAACTAACCTC850.16271056661562022No Hit
TTTGACAGGGCAATTGCAAGCAAATAGATTTTTCTTTGTAACAATTTGTTCT850.16271056661562022No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCG840.16079632465543645No Hit
TGGACTGACATCAGTTTGGGTGAGAGAGGGAGTCACTTTACTGAAAAGGCAG830.15888208269525267No Hit
GATCACGGGCTGGGGACTTGTTTTGGTTTGGGGGCAGCCATCCTCCACCAGA820.15696784073506892No Hit
AATGATAGGGATGTGATTCCAGCTCATCAGTTTCCAGATGAATTAGCTGCAT820.15696784073506892No Hit
GCAGTGGGGCTCGGGCATGTCTTTATTAGCAGTGTGAGAATGGACTAATACA820.15696784073506892No Hit
TGTGGATGGGAAGGCATGATTTTTGGATTGTTTTCTTTCCACTTGTTGGCGG820.15696784073506892No Hit
GTTCCAGGGAAGGAAATGAAGGCAGAAATAAAGATGTTCTTTGAAACCAATG810.15505359877488514No Hit
AATAACAGGGAAACTAGAAGCAATGCTTTCTTTTCTTGGTATAATTTGTCTT810.15505359877488514No Hit
CGTTCAGGGATTCATGACCATTTTTCACTACCAAGCTCCCCCCCTCCCGAAG800.1531393568147014No Hit
TCTGCTGGGCTAACTATTGGCCTGAAGACTGGCACCAGCTCACCTATTTATT800.1531393568147014No Hit
ATACTTGGGAAATATGGGAGGGGGACTCAAATTTCAGAAAAGCTACCAAGTC800.1531393568147014No Hit
TTTAGCGGGCTAACCAGGTCTTAAGAATAATGTAGAAGTCATGTTATATGGA800.1531393568147014No Hit
GTCAAGGGGAGATGTTTTTCAGAGGCCTACAAATACAAGTATCTTTCTTATA790.15122511485451762No Hit
AGTTGGGGGCGTGATGTCGTCACTTTTATGGCGTTAATTTCCTGCCACTACA790.15122511485451762No Hit
AACTACGGGGCCACTAAACACTGACTCGAATTCATATGATGCTGCTCACAGT770.14739663093415006No Hit
ACGATTGGGACAGATATGCAAGACAATGTCTTGTTTAATTTATTAAATTAGA760.14548238897396631No Hit
GGTATGAGGGGTCATAACAGTCTTATGAAGTAGTTCTTCGAATATAGAAAGT760.14548238897396631No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCCTGTCTCT760.14548238897396631No Hit
AGTCTAGGGATTTAAAAAAATGAAGACACTGAAGTACAGACTTAAATAGCTC750.14356814701378254No Hit
TCCCACATGGGAAGGTATGTGGACCGAGTGACTGAATTCCTCCAGCAAAAGC750.14356814701378254No Hit
ATTTTAGGGCCGATCTGGGTGTGGGGGTGGAGAGAGCGCGGAGACAAGCGCA750.14356814701378254No Hit
CATTGGGGGTCTGGCCTGGATACAATAAATTTCAGAATCAATATCAATAGAG750.14356814701378254No Hit
GAGATAGGGATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTACGA750.14356814701378254No Hit
AACATGGGGCAGGGGGAGAATGAAAGGGGCGAGAGCTAAAGTGTGTTGTCAA750.14356814701378254No Hit
GAATTAGGGAGAGTCACATTTTATTCTGATCAGAATTTTTATTGAGATGTTG740.1416539050535988No Hit
CTAGGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC730.139739663093415No Hit
TCTACGGGAAACATTTCTCAGTTTGTTTTTTTGCTTTTGGTTGATTTCTGGA730.139739663093415No Hit
GGTGGTGGGATTCCACCGACGTGGAGGCAGCCGTCAACAGCCTGGTCAATTT730.139739663093415No Hit
GATTTAGGGAATTAAGTCACTCCTGAGAAAATATCCTTTTTTCTAGAAGAAA720.13782542113323124No Hit
CTTCATGGGTGCCCTGAATTAAATGGCTGTTTTAACAGTAGGCTCTTAGCAT720.13782542113323124No Hit
AACTTGGGGGGCACTCGCGCAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGT710.13591117917304746No Hit
CCTTCTGGGGTTCATTTGAAGCCATTTCTGTAAACATCTTCCAAGATAGAAC710.13591117917304746No Hit
TACATGCGGGATAGGTATTGTGAAATATTTTGCTAATCTTATAGAAAAGGAA710.13591117917304746No Hit
AGCAGTGGGCAAATTTTTCATTATATCAGTCTTTTTGAAAGGATCAACTTGG710.13591117917304746No Hit
TGAATCGGGCTGATGCCAGGCACTACAATCTCATAGGGTTGCACCCTTGATA700.1339969372128637No Hit
GTGCAAGGGAGAGGAAAGTGGGGTAAAATGCTACCTGTACGTCTGACATGAA700.1339969372128637No Hit
TATCGGGGATAGGCTATGTCCTCCCGTGAGGCCAAATATCATTCTGAGGGGC700.1339969372128637No Hit
TTAGCGGTCCACACTGTGAAGGCAGTATTAGAAGACTTAATTGTAAAAGCTC690.13208269525267993No Hit
GCTTATCGGGGCGGATGGGAGAGTTTCTTTACATTGCCGTGGCATCTTAAAA690.13208269525267993No Hit
ATAAAGGGATGGCTTGGAGTTACCTTATATTCAGGTATATGTGATATTTTGC690.13208269525267993No Hit
GTAGTAGGGCTGCAGCTTGCTTCTCTGCAACAGAAATGACACTGCCTGGGAT680.13016845329249618No Hit
CCACTAGGGGTATAGGGAGAATTTCATCTATCTAATCCACAAGAATCTTGAA680.13016845329249618No Hit
GACACTGGGAGAATCCTTCATGTCATTTTCTCTTTTCTTATTCATACCAGAG680.13016845329249618No Hit
GATGTAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC680.13016845329249618No Hit
TGTCTAGGGGACCGACCCTCGACTTCGGAGAGGCAGCGCGGTTCCTCTGGGT670.1282542113323124No Hit
ATTGGAGGGTAATATATATACTTAATATTACCTCCAATGACTCGGGAATTGC670.1282542113323124No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCGTATGCCTGCC670.1282542113323124No Hit
AATCTAGGGAAGAACTGGGATTAATGAAGCATTTTGTTTCATTTACACAAAT670.1282542113323124No Hit
TAAGCCGGGGTATTACAATTAATTACTCTGACTGTAATAAAAGATATGTTAA670.1282542113323124No Hit
AATGATGGGCTTTCAGCAAAGAGTTAATGGTATCCATTATGACTTTAATATC670.1282542113323124No Hit
AGGCACGGGGAGCCTGCATTCTGCACACAGCATCTGCTCCCATCCCCCAAGT660.12633996937212863No Hit
TTTCATGGGATTGAAATTCTATCAATGCCTGTATCCCTGTAACCCCGAACTG650.12442572741194487No Hit
GGTTCAGGGGGAAGTGGACTGCGTTTATCAATACAGTCACAATGTTAAATGA650.12442572741194487No Hit
TGCTTGGGGATGGAAAAAAGACTTTTTGCCACTGAAACTTGAGCCATCTGTG650.12442572741194487No Hit
AAATTAGGGTTGATCTCATATTGCGTGATATATTAACAGATTTTGTAATATT650.12442572741194487No Hit
CTTAAAGGGGAAGATGTGGATACAGAATTAGTCACTCTTGTCACTTTATTTA650.12442572741194487No Hit
TGTCTTGGACCTCAGTGCTGCAAGTATCACCAGAGAGGCTATGGAAGAATTT650.12442572741194487No Hit
AGAGGTGGGCTCTTCCGATCTATTTGTGGGGGTGAAGATGCACTTGCAAATG650.12442572741194487No Hit
ATTAATGGGATGAAATGTAATGATGAAATTGAAATGAGATGAGTTGAAATGA640.1225114854517611No Hit
ACCTGGGGGAATCACTGCATTTTTAAAAAACAAAGTTCAACACAAATGACAT640.1225114854517611No Hit
GCTTTTGGGATGGGGAGAATTTTAAAATGTTAAACTGTAATTTGTATTTAAA630.12059724349157734No Hit
TAGTCCGGGGATCTGCTGCTGGGGACTAGATCCAAACTGTGTTGTTCTTAAA620.11868300153139358No Hit
TATTTTGGGCTTGTTATCAAAACCCTTGTCCAGCACAACCTCTTTTATATGC620.11868300153139358No Hit
ATTGCGGGGGTTGAAAGTCTAAATAAAAGGCAGTTTAGGTGCCCTGGGTGCC620.11868300153139358No Hit
TACCAGGGGACTTTTAAAACGGATGGCCTTCTTAAGAATAAAGCAAAAGGTG620.11868300153139358No Hit
GACTCTGGGAGAATTCTTTGGGTTCTTTGTAATTTAATGAATAGGTCTGAAA620.11868300153139358No Hit
TAAAGTGGGATAAAAATGTAGTTATAACTATTTTTAATTCCAAGGTGTTGTT610.1167687595712098No Hit
GTAAGCGGGATATTTAATGAAATTGGTGGTTTTAGGAAGTCAACTTTAGTTT610.1167687595712098No Hit
CATGCAGGGGTTCATGTACTCTGTAAGCTAAGTCATCAGCTTCCTAGTCCAG610.1167687595712098No Hit
GTTCGAGGGCGGACTCTCTGCTGCGGAGGGGGGCAATACCAGTGACACCCAG610.1167687595712098No Hit
TTAATAGGGGGGCTGGTTTTAGTACTCTGAAGGACAAAAACAAGCAAACAAA610.1167687595712098No Hit
CAAGTTGGGGATGCCCCTCATTTACATAAATATTATACTAGCATTTACCATC610.1167687595712098No Hit
TATCAGGGGAGTGATATTGTAACAATACTGCCATTCCCTTCTACTGCACTGC610.1167687595712098No Hit
TCTTCAGGGCACTGAAACTGCCTTATTCCTAGACTGAACTATTATACATGAA600.11485451761102604No Hit
GTACAGGGGTGCAGACTACAGTGGCCAACAGAGCACTTATGGCAAGGCATCT600.11485451761102604No Hit
ATAATAGGGAAATGTCTACTTCCAACATCCTTGAATTTGCAAGTGAAGGACA600.11485451761102604No Hit
GTATTAGGGACCAATTAATCAGTTAATTTCTGAAGACTTTGTATAGGACTTG600.11485451761102604No Hit
GCAAATGGGGCTCTCGCAGCCGCCGCAGTCGCTGCGCCCCGAGCCCCTCTCC590.11294027565084226No Hit
GACTTTGGGAGAATGGAATCCATGTGTTTGCAAAAAAAGTTTGCTAATTTTA580.1110260336906585No Hit
ACAGTAGGGAGGTGTTGAAGGATCTTTGATAGTTGAGAAAATTATGCAAAGT580.1110260336906585No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTC580.1110260336906585No Hit
GCATCTGGGTGGGCTGGATGAAGAGCAAAGTGAAGCCAGCACAGATGGGGAG580.1110260336906585No Hit
TTAATAGGGAAATTCTGATAGTTTCAAAATCTTTTATCTCCAGGTTTGATCT580.1110260336906585No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCGTAT570.10911179173047474No Hit
GCTCTTGGGATCTCCAGTCACAAAGAGAGACAGGAAATTTGGACTTTTAATT560.10719754977029097No Hit
GGATATGGGTGATGTCTTCAGTGTGGGACTGGGGTCCTCTTGGTTCTGCCTG560.10719754977029097No Hit
AATGTAGGGATTACCTCAGAAGTTTTTTTCTTCGCAGGATTTTTCTGAGCCT560.10719754977029097No Hit
ACAACTGGGTTAAAGGGAATATGGATAAGCATAGTAACAAAACCCACCAGAA560.10719754977029097No Hit
GTTCTAGGGAGTTTAATGTTTCCAGAAAGCCAAAGTAATTTTCTTTTCAGAT560.10719754977029097No Hit
TCGCTAGGGATTCTTTAGTGGGAATTAAGACCTACAAAGTCTAGTTTGTATG550.1052833078101072No Hit
CTACGTGGGGATCTCTTTACGGGATTGGAGCACATCAGTTTCTTGGGTAATG550.1052833078101072No Hit
TGTTCCGGGAGAAATGCCAATTTCACTTTGAAACCATAGTCCAAGTCTCTAG550.1052833078101072No Hit
TTCTTTGGGAATGATGATTATACCTTTTCAAAATTAAGTGACTTCCTAAGAA540.10336906584992343No Hit
AATGCCGGGACTTCAATTTTGCAATCGTTTTTACAGATGTTAAAAGACCTGG540.10336906584992343No Hit
TAGCCTGGGATAGCTTAGAGCTTTGTTCAATTCATTCGATAGTGTGATTCTT540.10336906584992343No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCGTATG540.10336906584992343No Hit
TCTAGAGGGACTTACAAGAGATGTGAAGGGCCTCTTCAAAGAGAACTACACA540.10336906584992343No Hit
TTTGCGCGGGAACATGTTCCATTCTAAGTCTTGCCCTTGCATGTGGTTGAAA540.10336906584992343No Hit
GCGCTAGGGGATACATAAGTTATATAAGAGGGCATAACAATAGATGAGTAGA530.10145482388973966No Hit
ACTAATGGGCAACCAGATTTAGGGCTCTTCTTAATTGATCATGTTCAGAAAG530.10145482388973966No Hit
GGTGTTAGGGATAGTGTTAGTGTGATGCTATACTATTGGAAAAATAGCAGCT530.10145482388973966No Hit
GTAGTAGGGAGGCATTGGATGCAGAACCTAGAGCCAGTTTTCAGGAACAATT530.10145482388973966No Hit
AGACTTGGGATTGTACCTGTGAGAGTGAATTAAATTTCTAGAGAGTCATAAA530.10145482388973966No Hit
TCATCTGGGAATGAAATTCAATTTTAGTATTTCTAATAATATTATTTCTGTT530.10145482388973966No Hit
ATAGCTGGGATGTTGATTGATTGGGCAACTGACACTTCCAGAATCTAGGTGA530.10145482388973966No Hit
TTCCGTGGGGACTGGCGCAGGCGCAAGCCGGCAAGATGGCGGCGGCTGGGGC530.10145482388973966No Hit
AAAAGGGGATTATAAGACTAGTGGAAGGGATACGTTTTTAATGATGGATGAG530.10145482388973966No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)