FastQCFastQC Report
Sat 31 Dec 2022
SRR5846565.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846565.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138513
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATGC5570.40212832008547933No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTTCCGATCTCGTATGCCGTCT4920.3552013168439064No Hit
ACTATAGGGGAAAAGTGATTTCAACTTGAGTATTTTATGCAGGGTGGGAGAC2530.18265433569412257No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTTCCGACTGTCTCTTATACAC2040.1472785947889368No Hit
TGACAGGGGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACGCCAAG1850.13356147076447697No Hit
TAGACCGGGCTGCTCACCTTTGTCTTGGTAGTGTGAAAGCAAACTTAGATAA1750.12634193180423497No Hit
GACTGCGGGGACGTCGGGAGCAGGATGGCGGCGGAGCAGGACCCCGAGGCGC1720.12417607011616237No Hit
CACTGAGGGTACAACTAAAACATAATGATATCAAAAAGTTAAAAGGAAGGAA1710.12345411622013819No Hit
TAGCTTGGGAATTCTTAAACTGTTCCAAAAAAATTGAAGACAAAGGAATTTT1670.12056630063604137No Hit
TTGCTAGGGATGGAAATACGAAATTGCAAATCTAAGTGAAAAACCTTTGTGT1660.11984434674001718No Hit
AAAAGTGGGACTGCCAGATGGCCCCGCCTGTTAGGACCGCCTGCTCTACCCT1640.11840043894796878No Hit
ATAAGCGGGATCAAAGAGAGACTTCTAAGACAAAAAGCATCATTAATGAGGC1610.11623457725989618No Hit
AATACGGGGAGTCCGGTTGCGCCGCGGCGTGAGGTGAGGTGTGAGTTGCCCT1600.11551262336387197No Hit
GCATGTGGGCACCTCTGAGCCTCCGATGCTCTGTGAAATGGAAGGAATGTGC1590.11479066946784779No Hit
ATGCGTGGGGAGTCATACATTTTTTTGGACTTTGTTATAATTCATTGTGGTA1580.11406871557182359No Hit
ACGAGGGGATTCAAGATTAACATAAATTTATTGTAGTCAAGAAAATGTAGTA1550.11190285388375097No Hit
CATCTGGGGACTTCAGTGCTCACCCGTAGGCAAATCTCTTTTTAAAAAACTC1540.11118089998772679No Hit
CTCTGCGGGGCACCGTGGCAGATCACTTCAATTAAACCGTGGTGATAAAATC1540.11118089998772679No Hit
GTTGGCGGGAGGTGCCCAGACATCTAGAGGGGCGCTCAGCAGATGGGAGTCA1540.11118089998772679No Hit
TACTTAGGGTTGTGAAATGTAATGAGATGTATCAAGATATCCTAGAAATAAA1530.11045894609170259No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACTTCCGACTGTCTCTTA1530.11045894609170259No Hit
GTAATAGGGAACCATGAGAAGTATGACAACAGCCTCAAGATCATCAGCAATG1520.10973699219567838No Hit
AAAGCAGGGGTTTCAGGCCCTTCTTGTACTTTCTCTACCTTAGCCATGGAAT1510.10901503829965419No Hit
AGAATAGGGGCTTTTTGTAGTGGTATTGTTTGCTCCCTCTTCGCGTTTTGAC1500.10829308440362997No Hit
TTTTACGGGGGGTCAAGGACCAGGGAGCCCTGACTCCCAGAGGCTGCCACCG1500.10829308440362997No Hit
GCTTGAGGGGGAGGGTATGGATATTTGCCTGAAGATAAAGTATTGGGAACTT1500.10829308440362997No Hit
GAGCAGGGGCATGGGAGGATACAGGGAGAAGATGGCCTTCTACGAATAAAGA1490.10757113050760578No Hit
GGATTAGGGTAAAAGGAAAAACTTGATATTTATCAGTCTGAGAAAATATTTT1490.10757113050760578No Hit
TAAACAGGGCACCATGCCGTATTAGACCTAAGTTCTTGCTCTTGGATGAAGT1490.10757113050760578No Hit
GTAGCAGGGGCGGGCAGATCGTTTGAGGTCGGGAGTTCGAGACCGGCCTGGC1490.10757113050760578No Hit
GTCTTTAGGGTTGGTATCATGTGTTTAGACTGCAGACAACGTAAAGGGATGA1470.10612722271555738No Hit
GCATATGGGCGAGCAAAATCTGAAACCCTGAGCATAATTTATGGAAGACCTG1470.10612722271555738No Hit
CGTACTGGGAGCCATAGTAAGAGAAATATGAGTGCTATCAATACATGAATAT1470.10612722271555738No Hit
ACGAGAGGGCTATAGTTCTTGTACATAGAATTTGCCCCATGAATATCTGTTA1460.10540526881953319No Hit
TTCTCCCGGGACCAGTTTCTAATCATAGATACTATCTTCCATTTCATTGTAT1460.10540526881953319No Hit
TTTGGTGGGTGACCTTCCTGGGCCGGGCCGCAGTGCTGGGTCAGTTCCAGCA1450.10468331492350898No Hit
GTCTTTGGGGTAGTTCTCACCAAAATATTGGAAAATGCCTCACTGAAAGGTC1450.10468331492350898No Hit
GTGTCAGGGATTGCTTATGAAAATTCCATAGTGGTATTTTTTTGGATTCTTA1450.10468331492350898No Hit
GACAAAGGGCCCTGCTCCGTGGCTGGTTTTCTCCGCGGGCGCCTCGGGCGGA1440.10396136102748478No Hit
GGTAAAGGGAAACCCAAGTCTTGAGCAATGTTTTTCTCAAAAAGCTGCTATC1440.10396136102748478No Hit
TTCGCTCGGGGAATGAGTGGGCTGTAGTTGGGCCTAAATAAATGAGCTGTTG1440.10396136102748478No Hit
TGAATGGGGTGGCAGGCAGGAAGCAGAGAAGCTCCTGCCCGCCAAACCCACT1430.10323940713146057No Hit
TAAAGCGGGGGCGGCGCCGGCGGAGCGAACATGGACCCGGCTGCGCGGGTGG1420.1025174532354364No Hit
TGTAAGTGGGACCTTAAGAAATTTCCTTCCATAGACAGCTGCCTCAAAGGGA1420.1025174532354364No Hit
AGAATCGGGCCTCGGTCTCCTGAGTAGCTGGTACTATAGGTGTGTGCCACCA1410.10179549933941219No Hit
TTGCTAGGGGTGAACCTTAGTAAGAAAGATAAGGTATTTAAGGGGCCTTTGA1410.10179549933941219No Hit
ACTAAAGGGGGACAGACTGCGGGACGGACGGTGGACGCTGGGACGCGTTTGT1410.10179549933941219No Hit
TTAACAGGGCCTCAGGACTTGAGGAATGATGCTGCGTTGAGTTCCTTAGGTT1410.10179549933941219No Hit
GCTCGCGGGAGGGAGACCGAAGGGCACAGCTCCCCGCGCCGCGCACGCCGCC1400.10107354544338798No Hit
TGCGGTAGGGAAATGAGTATTACAACTGGCTAATATCATTTTTTATATACAA1390.10035159154736378No Hit
ACTAGAGGGCAGCAAAGCATTGTCTTGAGCTAGTTCCTGGGGTGTGGAAGTA1390.10035159154736378No Hit
CTATTAGGGGGGGAAGGAGGCTGGCCAAATGCAGAACTTCTGAAAGTCCACT1390.10035159154736378No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)