FastQCFastQC Report
Sat 31 Dec 2022
SRR5846517.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846517.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83340
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGC47525.70194384449244No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCCGTCT37164.458843292536597RNA PCR Primer, Index 30 (95% over 22bp)
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCG10291.2347012239020878No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGA8721.0463162946964244No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATG5910.709143268538517No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCTTATAC5500.659947204223662No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCT4950.593952483801296No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCC3720.44636429085673146No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAT3640.43676505879529637No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGC3180.38156947444204464No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT2750.329973602111831No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTT1720.20638348932085432No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTC1670.2003839692824574No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT1590.19078473722102232No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGC1440.17278617710583152No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1380.1655867530597552No Hit
TTATTTGGGCGCTCTTCCGATCTTCTAGAGGGATCTCCCTGGATCTCACCTT1330.1595872330213583No Hit
GTAGCTGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA1260.1511879049676026No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCTGTCTC1260.1511879049676026No Hit
TGATAGGGGATGTTACCACATGAGGACCTAAGAAAAGGTGCATTGCTGATTT1250.1499880009599232No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCAC1210.14518838492920566No Hit
CACAGAGGGGAGAAAAAGCAGGGTAAAAAGATTTTATTTAATTAATTGAAAA1190.1427885769138469No Hit
ACATTGGGGCACCCACTTTTAAAAATCCACTCATGTCCATGGTATTCAAAAT1170.14038876889848811No Hit
ACGTAAGGGGACCTATGAAAGGAGAAGTGGTACCCGTTCCCGGGGCATCCCA1160.13918886489080873No Hit
CCTACTTGCAGATTTAACAAAATTTTAGGGAAATTGAAAAAGACATGTAGAA1160.13918886489080873No Hit
GTCGATGGGGTTTTTGTTCTCCCTTTCCCAGTGTTAATGGGGGACTTTGAAA1140.13678905687544998No Hit
GAAGCGGGACCAATGAATCAATGAACATAGGTGAGTGAGTCAAGGCAAATTT1110.1331893448524118No Hit
ACGCCGGGATAACAGATATAATAATGAAAAAGATTGAAATGTGAGAATTACC1110.1331893448524118No Hit
GCCGCTGGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAAT1100.1319894408447324No Hit
TCGACTGGGGCTGATATATCATACAATTGGCATATAGAGCTTCAGTTCATTA1100.1319894408447324No Hit
CGATATGGGATCATTTTGAATTGCTGACTGTTATTGTAACTACATGTAAACT1080.12958963282937366No Hit
AGCGATGGGTGGGATATTAAGAATTTATCTTAAAGGGTTTTTGTGAGGATTG1070.12838972882169428No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1070.12838972882169428No Hit
TCGGTCGGGGAATATTCTTATGGATGACACTAAATAATTCCATTTAACAGTT1070.12838972882169428No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGCCGTCT1050.12598992080633548No Hit
TGCACTGGGGTTGGAGATGTAGTAGTGAATAAATCAAAGTTCTTGCCCTCAT1040.1247900167986561No Hit
GCGTGAGGGATACAAGTTTATGTTTAATAATTTTTTGGAAATTACAGATTTT1040.1247900167986561No Hit
CGCCATTCCAATGGGATAAAAATAAAATAAAATAAAAGCATATATGTTGTTA1040.1247900167986561No Hit
CTACATGGGATTGCTGGTTATGATGATATGGGCCCTCACATTTTCCAAACCT1030.12359011279097672No Hit
AGGGCGGGGGATCAGGTGCCTATAAATCCCTCTCTTTATCTGGTGAAATATG1010.12119030477561796No Hit
CGCTCGGGCGCCTCACTGTTGAGCCCTACCCCTTCCAGTATATTGTATTTCT1000.11999040076793857No Hit
ACTGTGGGGACATAATTATGAGTTTGTTCATATAAAGAAAAGCTGTGGAAAA1000.11999040076793857No Hit
GGCTACGGGAATAAATAAATCAACACTTTCAGTTTTCTGCCAGTGTCACAGA990.11879049676025918No Hit
TCAGGGGGTACTATCTTTATTTTTATTTTATTTTATTAAAAAACTTTTTTTA990.11879049676025918No Hit
ACGACAGGGGTTAGAAATTTAGGCTATTTCCAGATTTTCAGTATTGTAAATA980.1175905927525798No Hit
ATGTTGGGGCTGGGAAGTCCAAGTTTGAGGGGTGAGCATCTGGTCAGGGCCT980.1175905927525798No Hit
GCTATGGGGTCCTGAATGGTATTGCCTAGATTTTCTTCTGGGTTTTTATAGT970.11639068874490041No Hit
CGATGGGATTCAGGTAACTGAAGATTTTGTGTCTCCAGAACATCTTGTAGAG960.11519078473722101No Hit
TTCGGGGGGATGGCCACTGAGAGAATTCAGAATAAATTGAAAGATGGAGTCT960.11519078473722101No Hit
GAAAAGGGGGCCTTTCCGTCTGGCGGCAGCCATCAGGTAAGCCAAGATGGGT960.11519078473722101No Hit
TTTACTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC950.11399088072954162No Hit
GACGTCGGGACATCATACTTGGACATATCAAGCATTGGTGCACGATGTACTG950.11399088072954162No Hit
CGCTCTGGGGATTATAAGACAAGGTTTCAAAAAAGTTGAATAGAAGATCCTT950.11399088072954162No Hit
TTCTGCGGGATGAGTGGGCTGTAGTTGGGCCTAAATAAATGAGCTGTTGGAA950.11399088072954162No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGCCGTCT950.11399088072954162No Hit
TCTGAAGGGGAAACATTCCCTTATGTAAGAGGTAATTAAAGATAGTGTCTTT950.11399088072954162No Hit
CAAACAGGGATTATTTAAGTAGTACATCTATTTAAATAACACACTAATAGTA940.11279097672186225No Hit
TGCTTGGGGGAAATAAAAGCATTATTGGGTGCCTTTGTTTGTAAACCAAAAA940.11279097672186225No Hit
CTTGACGGGTAGATAGACCTGAGTTTCAACCATAGCTGCACTATCGTTTAAC940.11279097672186225No Hit
CATAAAGGGCAGAACTCTACCACGAGCTCAACCAGAAGTTCCACACTTTCGA940.11279097672186225No Hit
AATATCGGGGACGAATTATGCTTTTAAATCATCCTGACAAAGGAGGATCTCC930.11159107271418287No Hit
CAATATGGGACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT930.11159107271418287No Hit
TCCAGAGGGGTTGCTCAGTAATCAACAGCTACTACAACAATCTTAACAGTAT920.11039116870650348No Hit
GCTTAGGGATGGAGATGAATGGGAAGCTGTTTGATGAGCTCACAGCCTCCTA910.10919126469882409No Hit
ATAACTGGGATAGCATCTTCGTATAATGTTATCTCATAATGTTGTTGCCAAG910.10919126469882409No Hit
AGCTTTGGGATCTCAAATATGTGATACAGTGCATAAAAATTAAGAATTAATT910.10919126469882409No Hit
GTGAGGGGGTATGAGCTTCTACAGGTATTTTTAAATAGAGCAAGCATGTTGA900.10799136069114472No Hit
TTTGGTGGGATACTTGGTGGGTGAATCCAATTTTGTAGAATTCCTACACAGG900.10799136069114472No Hit
CAGTTTGGGGGATTTTCAGATCTTTTCATGAGAACTGGAAATGTAGCTGGGT890.10679145668346533No Hit
CTAGATGGGATGGAAATGAATATGTATTTTAGACACACATTTACCTTCTTTG890.10679145668346533No Hit
TTTGAAGGGGTGCAGGGATTTGAAGAAACAAATAAATCCGAGAGAACTGAGA890.10679145668346533No Hit
CGACTGGGGCAGTTAGTCATGCTCCAAGGATTGAGGTCATGACCATGGGAAT890.10679145668346533No Hit
ATAAGGGGGAAGAAGACTTATTTTTGCATACTGGCACTTTAAGAAATTACAT880.10559155267578593No Hit
ACCCATAAAATTATTTGAAAACTTTCACCAGAAAGGGATGTAAGTCTCGCTT880.10559155267578593No Hit
GCTAGAGGGGGCTGCGGCGGGGACCTGACAGCAGGCAGGGACCGCTTCAGGT880.10559155267578593No Hit
TGGTCTGGGTTACCCTTAAGGTAAAGTCCAGTAGTAACATGGCTCCAACCTC880.10559155267578593No Hit
GGGTTTGGGAAATGTGGAAACAAATGGAAAAGTGGGCTTACCACTAATACAA870.10439164866810655No Hit
ATCTAGGGGGTCCAGTGTGGGAAGCAAAAGGAAATCACGGTATAAGTTTTGC870.10439164866810655No Hit
AGAAGCGGGTAGGGAAGGGTAAATACATGTACAATTGCTTTTAGTTAGCAAT870.10439164866810655No Hit
GCTCAAGGTTATCTTTTTATAATTAAGTCAGTTCAGTTTATTTAATAGATAT870.10439164866810655No Hit
CTTGGTGGGCTTTTGTGGATGTGTAATATCTTGGGTAAAAATCATGGTGCCA860.10319174466042716No Hit
TTTGTAGGGTGGTTATGGTGGTCAGAGCAGTATGAGTGGATATGACCAAGTT860.10319174466042716No Hit
GCTAAGGGAATCTGATGTGCAAATTATAATTTAAATATGTTAATTTTTTTGC860.10319174466042716No Hit
GCTGTTGGGATGAGAAGCATTTAATAAATTACACCACTGCATTTTTCCAAAG860.10319174466042716No Hit
CTTCAGCCAGACCCAAGCTCTTTCATTTCAGGTTCTGTGGCCAAGAACTTCT860.10319174466042716No Hit
AAACCAGCATTCTTCCCTGCTATTTTTTTCTAACTTTTGTCACCATCTGACA850.10199184065274777No Hit
GTGATGGGGGACAATTTCAAGTGAGACAGGTAAACATTTTTAGTTATGTGAT850.10199184065274777No Hit
GTCTCCAGGAAGTGACTCCCGGGCAAGGCAGACTTCAGTAATCCCTGAAGCG850.10199184065274777No Hit
CATCTTGGGCTCTCTCTTCCTCCATTAGAAAATTAGGTAAATAATGGATTCC850.10199184065274777No Hit
GATACAGGGTCTGCTGTGAGAGGCCGTTTCCATTCTTTCCTGCTGAATATTT850.10199184065274777No Hit
ATCGCTGGGATAATTTAAAATTACATGTTTGTAAATTCACCTGCTCTTTTAT850.10199184065274777No Hit
TCCAAAGGGACCGTCAGGCACTGGTGTTGATTCCTCAGAACGCATCCACCTA840.1007919366450684No Hit
AATATGGGCAGGTCCACAGATGCATTACGAAAAATACAGGAAAGCTATGGAG840.1007919366450684No Hit
GCGACGGGGAGCCAGAGCCTCCTCCAAAGGAAAATTGCAACAGTATATACTA840.1007919366450684No Hit
TAGTACGGGCATCTTAAGAAAAAATATCCATTTTTTTCTTAAGTTCGGGAAA840.1007919366450684No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)