FastQCFastQC Report
Sat 31 Dec 2022
SRR5846514.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846514.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92741
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGC65537.0659147518357575No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCCGTCT51355.536925415943326RNA PCR Primer, Index 30 (95% over 22bp)
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCG16151.741408869863383No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGA10311.111698170172847No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCTTATAC7760.8367388749312601No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCT6630.7148941676281256No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATG6340.683624286992808No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAT5120.5520751339752644No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCC4280.4615003073074476No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGC4080.43993487238653883No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT4040.43562178540235713No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT3210.34612523048058574No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGC2080.2242805231774512No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTT2070.22320225143140573No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCTGTCTC1830.19732372952631522No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1820.19624545778026978No Hit
GCTTATGGGATAGGATGGTCAACGTGTAACAAGACTGTGCTCAGAAAACGAA1720.1854627403198154No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTC1590.1714452076212247No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGCCGTCT1490.1606624901607703No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1460.157427674922634No Hit
CTTAAAGGGATTTTTGATAACTGTGTACTTCTGGTGACTGTACAGTTTGAAA1450.15634940317658858No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCAC1400.15095804444636138No Hit
CTATGCGGGGAAACCCAGTGTGGAGAAGGCACATGTCTGTGGCTCTGAGCTG1390.14987977270031594No Hit
GCATCTGGGAGTCGAGGTGAGACTAGCATCGAAAGGCACATCAACAAGAAGG1310.14125359873195242No Hit
GCGAGTGGGGAGGAAAATGAAGCTCGGGCTGGTTAACTGACTTGCTCAGCGT1270.13694051174777067No Hit
TTTGACAGGGCGATCTAGCAAAGGGAGAAGGAGATGATCAAGAAGGTGAAAA1230.1326274247635889No Hit
GATAAAGGGAGTGCTGAATATCAAGAGTGTACATATCATCCAAAAGAGATGT1220.13154915301754347No Hit
CAGTACGGGAATCACACATCTGAGAAATTACTTTCACAAAACGCTCTACAAA1220.13154915301754347No Hit
CGCTACGGGGCGTATGACAGTATGCAAAAATGAAGACTATTGTGTAAAATCA1210.13047088127149806No Hit
CCCAAACCTAGAGAAAGATGTCAGCATTGAAGTACAAGAAGGTTATAGAACT1190.12831433777940718No Hit
CTCACAGGGCAACAAAAAGTTGTTTAAAAAAAAGATTAAAAATTTGATAACT1180.12723606603336174No Hit
ATAAGCGGGCCTGAATTCAGAGCCCTTCAAGCTCAGGAAACCACGCTTCACT1160.12507952254127086No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTAT1160.12507952254127086No Hit
CACACTGGGTCAAGTGGGTTTTATCCCAGTGATGCAGTGATGGTTTAACATA1150.12400125079522542No Hit
AAGTTAGGGTGGGATTATGTGTTGAAGCATAGTCCTCATGCTTAATAAACTG1150.12400125079522542No Hit
CAATCTGGGGGACCCACTGATCATTCAGAAGCATTTTGTTTAATTTCCATGT1120.12076643555708909No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCG1090.11753162031895277No Hit
ATTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATC1080.11645334857290734No Hit
AGCTTAGGGGATTCTCAGCCTGGTAGATGTTGGTGTATAGCACTGCTACTGA1060.11429680508081647No Hit
GCGCGGGATCTAATACGGGGACCTACTGAGATTATTTGTAAGAATTGGAGCA1060.11429680508081647No Hit
CAAAGAGGGGTACCTAATAAGAGATATATGAATGGAAAAACAGTTTTTGCTC1060.11429680508081647No Hit
ATTTTTGGGGAGATATCTTCTCACTCTGATTAAAATGGCTTATATCCAGAAA1050.11321853333477103No Hit
GACTTAGGGTATTTATATTGATGACTTACTCCTTTTTTGTTGAATTGTACTT1040.1121402615887256No Hit
GTTGCTGGGATTACAGGCGTGAGCCACTGCACCCAGCCCTATATGAATGTTT1040.1121402615887256No Hit
TTTCTTGGGCTATTCTTCAACCACCTCGGTTACTCTGACATAGGAATTTACT1040.1121402615887256No Hit
CTCGACGGGCATGGGAGGCCAGGGTGTGTCCTGAGGGGAGGGTGGGGCAGGA1020.10998371809663471No Hit
CAAACGGGGAAGCTTCAATAAGGATTGTGTGTAGGTGAATAAAGACAGCTAA1010.10890544635058928No Hit
CATTTAGGGATTTTGAAAAGACGTACTTGAATAGTTACAGCATATGTTTGAA1010.10890544635058928No Hit
TGGCTTAGGGGTAGCCTAGGATCCTCCTGGTGTAAACTGTGCATTTCATACA1010.10890544635058928No Hit
CGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCG990.1067489028584984No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGCCGTCT980.10567063111245296No Hit
GCGTCAGGGGTGGTGTGCAAAACTCCGTACATTGCTATTTTGCCACACTGCA980.10567063111245296No Hit
AACACCGGGGAAGAGGTGGGGAATCATCTTATAATTTTATAGCTGATTATAT970.10459235936640751No Hit
GCTAGGGACTCTCGTGAGTCTAACGGTCTTCCGGATGAAGGCTATTTGAAGT960.10351408762036209No Hit
TATATAGGGAAGATCACCTCCTCTCCCACAGAGGCTGGAGTCAGCATAAGTG950.10243581587431663No Hit
AGTTATGGGATCTGAAGAAGCCCCAAAGCCCCACCGGTGCAGAGGAGTAAAA950.10243581587431663No Hit
AGCGATGGGCTCAAGATGTTAACTATAAACACACTGCATACAATTTTCTTCT940.10135754412827121No Hit
TTGCTTGGGACTGACCGCCGCCTGAATGTGTGGGATTTAAGTAAAATTGGGG930.10027927238222577No Hit
CACCATGGGATATGGTCTTGCAACAGTTTTAAAGCCTCTGCATAATTGATAA930.10027927238222577No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)