FastQCFastQC Report
Sat 31 Dec 2022
SRR5846494.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846494.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104658
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGC50784.851994114162319No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCCGTCT42664.07613369259875RNA PCR Primer, Index 30 (95% over 22bp)
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCG13321.2727168491658545No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGA9360.894341569684114No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCTTATAC6330.6048271512927822No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCCTGTCTCT6080.5809398230426723No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAT3980.3802862657417493No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGC3880.37073133444170536No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGT3210.3067132947314109No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATG3050.2914254046513406No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCGTATGCC2320.22167440616101972No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGC2300.21976341990101092No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTT2230.21307496799098016No Hit
AGATCTGGGCTAACAAAATCTTTGTAAGTTCTGTATGCTGAAACCTACAGAA1770.16912228401077797No Hit
CTAATAGGGCAGTTTTCTCTTTTTTATATTTTCTGGGGGTTTTTTGTTGAGA1640.15670087332072083No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTCTGTCTC1630.15574538019071643No Hit
GCTCAGGGAGTATCGTCTGCATTCCAGCAAAAATATCAACTCGTAAGGCACT1600.15287890080070324No Hit
ACTAAGGGGACTTCTGGTGGTTACCCTGACTCTTGACTCTTAGAAGTGCCCG1560.14905692828068567No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGACCTCGTATGCCGTCT1500.1433239695006593No Hit
AGAACTGGGGGGCTCCGAAGCGGGGAAGTGGGACAAGATGGTTTACATCTCG1480.1414129832406505No Hit
GAGTAGGGGGGGCATTCACCTAGTCTTAATTGAATGCTAGGTACTCTACATG1470.1404574901106461No Hit
ACAATAGGGCAAAGCATAATGTGTAGCATATCAGCAATAACTACATATTTAT1470.1404574901106461No Hit
CCACAGGTAGGCTTAGGTTTATGTGTGTGTATGTGTCTTAGTTTTTAACAAA1420.13568002446062413No Hit
GTATAAGGGAGCATGAAGAGGAAAAAAGACAAAAACTAGAATTCATTGGAAA1420.13568002446062413No Hit
TAAAACGGGGATCCATTTCAAAGCAGAAGCTGTGGAATGGTATTAAATATTT1410.13472453133061973No Hit
GTTTTGGGGATCAACAAATATGGCTGCTAGTATCTCTACTAGTATAAGAAAA1390.13281354507061094No Hit
AATTTAGGGGATTTTTGTGTAGACATATGTTTTTATTTTTCTTGGGTAACTA1390.13281354507061094No Hit
CTTTCACCGGGATAGTGATACTTAAACAGGTGAGGAGGCAATGCTTTTCTAT1370.13090255881060214No Hit
ATGGTTGGGCTATGAGAGATACAAAAATGAATTAGACACAGGTTTTGTTGTC1360.12994706568059775No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1340.12803607942058895No Hit
GGCATTGGGCCAAATCCAAAAGCTTCATTTTTATTTTTGGTATTTAATAAGA1340.12803607942058895No Hit
ATATGAGGGCAAAAGGAAGTGACATCACGAGTGGCCAGACTGAAATTTAGTG1340.12803607942058895No Hit
TATTTCGGGGAAGGGTGAGCTGTACATCATGACTTCTTTCTAAGGGTGCAGA1330.12708058629058458No Hit
GATAGTGGGATGGATATAATTTGCAGCCCATGGATATAACTGGTTGATAAGC1330.12708058629058458No Hit
TGTTTGGGAAATCATGCATGCAGATTAAATCTTTTACAATAAAAAATTCTCT1320.12612509316058018No Hit
CACGGGGCACAAAAATTAGCTGGGTGTGATGGCGTGTGCCTGTTAATTCCAG1320.12612509316058018No Hit
GAGATGGGGTATGCCTGTGAAATTTGAGAATGGAACTTGGGTGGTTCTTACA1310.12516960003057578No Hit
AACGCTGGGGGGTTGTTTATTTGAAGTTTTTCTTTTGTGATGTAGGCACTAA1310.12516960003057578No Hit
ATCCTGGGGATTTTCACATTTGACATTTCATTCACATTCATTTCACATTTCA1290.12325861377056699No Hit
TCATGGGGGACCAGCCAATGCTGGTGCAATGGGAAATATAGTAGGTATAAAT1290.12325861377056699No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCAC1280.12230312064056259No Hit
TTCTAGGGGTGAGGAAGGAAACTATGAGGAGGAAGGAAGTGAAGATGTCTGG1280.12230312064056259No Hit
CTTCTTGGGCAAAGAAGGGGCAAGCTCTTTTGCCTTTTAGGCCAGACATAGC1270.12134762751055819No Hit
GGCATTATCAGGTACCTCCAATAGTGGGGAGATGGTTTTCTTTCTGAGGGAA1270.12134762751055819No Hit
AATCTCGGGCATGGGTTTATTGCTAGAAAGATCCAGGGGATAGCTTTAGGTT1260.1203921343805538No Hit
ATTACAGGGCGATCTGTAGGCGGGCCAAGGAGGCATCGCAATACTTCATCCT1230.11752565499054062No Hit
GTGGATGGGGAGACGAGGCTGCGCCCGGATTCCGGTCCGCAGGGAGACCGAA1230.11752565499054062No Hit
TTTAGAGGGCATGGAGTTTATTTTATATCATATTTCCCTTTTTTATTTCTGC1220.11657016186053622No Hit
TAACAATGGGAAGTAAAATTATATTTTCCATCATCTTTTATGCTAAAGGAAT1210.11561466873053182No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATCTC1210.11561466873053182No Hit
ACTACAGGGCAGAGTTGAAAATGCTTTTAGGTGGCATCATAGAACCATCCAT1210.11561466873053182No Hit
GTGACAGGGGGTCCAGCTCAGCTGCCTGGCTGCGACAGCAGGGTGCCACCGA1210.11561466873053182No Hit
TCCTAGGGGTACTCTTCCAAAATACATCATTTGCTGCCTGACTTAGACCTAG1180.11274818934051865No Hit
GCAATGGGGATTCTTGCCAGTGATAGACAACAATTGAAAACCATGATGTTTC1180.11274818934051865No Hit
TAATGCGGGAAGACTCCATCTCAACAACAACAACAAAAAGGAATTAAGGAGG1180.11274818934051865No Hit
CATGTAGGGCATCCAGTTTGGTTGCTGTGGAGCAACATGCATATTGGATTAG1170.11179269621051426No Hit
ATAAGTGGGGATGTCCCTGCATGGAGCTGGTGAATTACTAAAAGAACTTGGC1170.11179269621051426No Hit
TTAGCTGGGAGTACAAATATTAACAATTATATTAACACCTGCCTCATGTCAG1170.11179269621051426No Hit
CACGCAGTGGGCATCCTTTTCCTCTATGTAGTCATACAAACAGTTTGTATAT1160.11083720308050986No Hit
TAGGGTGGGCTGAGTACTTTGAAAACATAATAGTTATGAAATTGATATTATA1160.11083720308050986No Hit
GTAAGTGGGACAAGGCAAAAACTATTGAACAGTTGGTTTTAGTGTGTTGTAT1150.10988170995050546No Hit
ACAATGGGGAGTGCATAGGCTGAAATGTCTCAAGATTCCCCACACTACATAT1150.10988170995050546No Hit
GTTAATGGGCAGTGAGCTGTGATTGTGCCACTGCAGCCTTGACCTCCTGGAC1150.10988170995050546No Hit
AGCGCTGGGAAAATAGAATTGATTAATTCTTCCTATGATCAAATAAATAGGA1150.10988170995050546No Hit
GAGCACGGGATAGAAGTAAAAAAACAATAATGCATATTTCCTCATATCATAA1140.10892621682050106No Hit
ATTCATGGGGGTCCCAATGCTGGAGCTCCTCTGGCAGGTTCTAAAAGCACAC1140.10892621682050106No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCACCCGAACTCGTATGCCGTCT1140.10892621682050106No Hit
TGTATTGGGATTCATTCATTCTTTTAGCAAACCAAAGACAGTGTATATATGT1140.10892621682050106No Hit
TAATGAGGGGTAATTTAGTAATATGAATATAAATCTTGTAACTTTTAAAATT1140.10892621682050106No Hit
GGTTGGGAAGGAGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTA1130.10797072369049666No Hit
ATAGCGGGGCAGAACAGCATGTGGGCTCTGGGCCTTGGCAGGTGCACAGACG1120.10701523056049227No Hit
ATATTGGGGAGGCATCCAGGGTGTATCCTGCCACTGTGCAGTGCCCTGCTTT1120.10701523056049227No Hit
TTGCCGGGGATTGATGAATTTTCATAAGGTGGAATTGATCTTAATCTACTAA1120.10701523056049227No Hit
AACATGGGGAGCTGATGTCAATGCTATGGACTGCACAAAAGTCTCAATGTTC1120.10701523056049227No Hit
TCCGTAGGGATACATATATATGCACCCAGCATTGGAACACCCTGATACATAA1120.10701523056049227No Hit
CGGAGCGGGGTGACCTACAAAGAGACTTAGACTCCCACACATTAATAATGGG1120.10701523056049227No Hit
TTTTATGGGGTTTAAATTATGAACAGAATATGAGTGATGCCATGGCAATTTT1110.10605973743048787No Hit
ATCGCTGGGAACAAACAAACAACAATAACAAAAAAACTGGTTTACTGAAAAA1110.10605973743048787No Hit
ATCATACGGGTTATGACTTAAACCAACTACAACTTCCCTATAGCTTCTAAGC1110.10605973743048787No Hit
AGTTATGGGGTTTTACCTACTTTAAAAGTTTTAATGGATTGCACCTCTGTAA1110.10605973743048787No Hit
ACCGTGGGGAGTGGTACTTGGAATCCTGTTTTAATTTGCACAAATTTAAATG1100.10510424430048347No Hit
AAGAATGGGTATGTAGAATGGATACTCATACAATGTTTGAAATTTGAAATTA1100.10510424430048347No Hit
AACGCAGGGATATTAAATATGCTACTGACTTTGAAATGCCTACAGATTTGCA1100.10510424430048347No Hit
GCCAGTGGGCAGATCAAAAATTCATTTAATTGTATTACTGTTTTGTTCCTCA1100.10510424430048347No Hit
GCTGCAGGGATATTTAAGTTGAACTTTATATATTGAATTCTTCGTTCTATAG1100.10510424430048347No Hit
ACCCATGGGAAAGATGCCATTAAAAAGAAATTTACAGGTATTAAACATGAGT1100.10510424430048347No Hit
GCCCACGGTAAGAAATTGGACTACATAAAAATGTAAAATATGTTTACATTAG1090.10414875117047907No Hit
TCTCTTGGGAACATAATGGGCAGATTTTCTGTCTTCAAAGTTTCCGGCTAAA1090.10414875117047907No Hit
GTAATGGGGAGAGGATGATGTAATTAAAAAATGGTCAGTGTGCAAAGAAAAG1090.10414875117047907No Hit
CTTGCTGGGAGTTTCACTAGAATAAGACCATGCCTGTTGACTAAGTTCAAGA1090.10414875117047907No Hit
TGGCATGGGATAGCTGCTTTTATGTGTATTTTATATTGCATGCTTTTGTAAA1080.1031932580404747No Hit
TCAACTGGGAAGTTATGGGTGGAGAAGCCTGGGTATGACTTTCCGTTGTGTA1080.1031932580404747No Hit
TCAGCTAGGGGAGTGCTTATTGAGTAAAGCATAATTCGGTCATAATGAAATC1080.1031932580404747No Hit
GAGTATGGGATATAAGGTGGCATATGTTAAGTTTTGCTTCATTGGACCGTTT1070.1022377649104703No Hit
GGGCGCGGGAACCACGGCGGTGAAGCAGTGGGAGCAGCGCTGGATCAAGAAC1070.1022377649104703No Hit
ATAGCTGGGGGCCCATGGGGGGATAAAAATGTCAAATTATATGTCTTCCAGT1070.1022377649104703No Hit
ACTCAGGGGCTATCAGGGATGGTTGAATGTAGCATTTATGGTTGAATGTAGA1070.1022377649104703No Hit
GCGACAGGGGTTATTGGTGATGCTCTTGCATTTAGTCATAATTTGGAAGATG1060.10128227178046591No Hit
TAACAAGGGATGACTGCCTCTCTCACCTAACTGCCAGTATTAGCTTTTCAGG1060.10128227178046591No Hit
ATTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCCGATC1050.10032677865046151No Hit
TCAAAGGGAATTATAAACACAAAATTGTTAGTGTTTATGATGTTTTTATCAT1050.10032677865046151No Hit
GCAGCAGGGATAGCAGAGAGAACTATGTAAATTATCTTGCAGAACAAAATAG1050.10032677865046151No Hit
ATATACGGGGATTATCTACCGGGACCTCATCAGCCACGATGAGATGTTCTCC1050.10032677865046151No Hit
ATGTATGGGGAAAAAGAGGAGGTACCAGCCAACAGGAAGACCACGGGGAAGA1050.10032677865046151No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)