FastQCFastQC Report
Sat 31 Dec 2022
SRR5846425.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846425.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144392
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTACATCAATCTCGTATGCCGTCT10700.7410382846695107No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACATCAATCTCGTATGC10260.7105656823092692No Hit
ACGTTAGGGCTGGGTAGCTGGGATTACCGGGGCCTGCCACCACACTTTGCTA2260.1565183666685135No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG2220.15374813009030971No Hit
CATCAAGGGGATTTGTTGGAGTATGATATTTGCTGACAGCACTATATACTGG2080.14405230206659647No Hit
GTTGCAGGGGGCTAGACTTAGATTCATTAACTCAAATTCAATGCTTCTATCA2050.14197462463294366No Hit
ACGCCAGGGTTATGATACTGATTTTCCTTTAATGTTAATATGCCAGAAAGCA2040.1412820654883927No Hit
GTTTGAGGGATACGAAAATTAGCTTTTTGTCCTTTTTATTTAAAAATGTTTT2020.13989694719929083No Hit
CGGCTAGGGCACCTAACCTCTTATTGAAAGTAATTCAAAGTTATTTTCAAGC2000.13851182891018893No Hit
AAGGTAGGGATTTAATGAGGAGTGTATCTTACCAAACAAAACAATGGAAATG2000.13851182891018893No Hit
CCATTAGGGAGCCAACGTCACTCACTAGGGAAAGGCAATTGGTAAATCCACT1990.137819269765638No Hit
AGCTACGGGAACCAAACTGACTTACTGAGTCTTAGTGGAAAAACACTTTGTG1970.1364341514765361No Hit
CGAGTAGCGGGGAAGCTCTTGGGATCCTAGAAGTATTCTTATTTTCTTCTCT1970.1364341514765361No Hit
AACTCCGGGAAGTGAGATCGTGCAGTATTTGTATTTCTGTGCTTGGCTTATT1950.1350490331874342No Hit
AGGAAAGGGAACCCTGATGATGATGTCAAGGGTCTTCTTCTGCTGTGTGGGC1930.13366391489833232No Hit
ATGAATGGGCTAGTTGTTTCTTGAAATCAAGCTGGCACGTGTGACTCCAGGA1930.13366391489833232No Hit
AACTCCGGGTGGACTGTGGATACTGAATCAGTGTACTATTGGCTGCAGAATT1910.13227879660923042No Hit
ACACACGGGATTCAACCTGAGAAAACTGATTAAAAAATTAGTTTAAATTTGC1910.13227879660923042No Hit
ACTACGGGGAAGAATGGTTCTAATTCACAGAGGTCTATTAGGCTGTGTTTAC1890.13089367832012855No Hit
GAAACCGGGTGAGACATGCATTTTCACGTGTGGCCAATTGCACAGATGCGCA1870.12950856003102665No Hit
TACAATGGGTGTAAGGCCATTAAATAAAAGCAGGATGACTTAGATATTTTTC1870.12950856003102665No Hit
CATTGCGGGACTTACCTAAATCCTGGTAATAACAAATGGTTTTTGTAAAGTG1870.12950856003102665No Hit
CTTACAGGGATTCAACAAAACCTTCAGGAAGCCCTTTGGAAGAAAAACACGA1860.1288160008864757No Hit
TATTTTGGGCACTCAGTCACCATGGCTCCGTCTGTCAAACTCAACTCTTTTT1850.12812344174192475No Hit
CCAAAGGGGGAGAGAAAGCATTTTCCAAGCCAGGTTCACTTGGCTGCAAAGC1830.12673832345282285No Hit
TAGAATGGGGTGTATAGTGAGAGCACCTCACTGGCAGAAAAACTGCTTTCCC1820.1260457643082719No Hit
GCATGGGCTGCCAGTAGCGTAACAGTCATATTTGCTTTTATTCACATTCACC1820.1260457643082719No Hit
CAATACGGGCTTGTAATGTCTTTGTTAGAAGGAGTTTATATTAGGATTATGC1820.1260457643082719No Hit
GTTGCTGGGAAGGTGTTAGCTGCATTCACAACCAGTCTAATCATTTGTCTCA1820.1260457643082719No Hit
TAAATGGGGTGTTCTTGTCTTTTACATTACAAAGGTTGGTTTAAAGCCAGGC1790.12396808687461908No Hit
TTGAGGGGATAAAAGAGTGGCTTTAAAATTTGAAATACTGGGAAAAATATTT1790.12396808687461908No Hit
GTAGGTGGGAGAGGTGGGGAAGAAAATAATTTAGAAAGCCTACATCGACTTT1790.12396808687461908No Hit
ATTCAAGGGTGCTACCAAATGGCAAAAAGCAACAATAAACAGTTTGATTTTT1780.12327552773006814No Hit
ATACAAGGGTTATATGGCAAATAGCATCATCTTGAATTTTGTGAAATTATTT1780.12327552773006814No Hit
CACTAAGGGTACTAAAGCAAAAATAATTTAGGGTCTTATCCTGGGTAAAATA1770.1225829685855172No Hit
TACTTAGGGACAGGACGTATTCTTCCATCGTCTTATATATAACAAGCTGTTT1760.12189040944096627No Hit
CGTTTTGGGGTAGGTCCTCTTGGGATTGCTAGTTTGTTCTTGCAGTCCATCT1750.12119785029641532No Hit
CGATGGGTAAAAATATAGGTTTTCCAGCACTGGGACTATGAAATTTGAGATT1750.12119785029641532No Hit
ATGAGCGGGTAAGGGAGAAGAAGAGGTGAGAGTTTGTCAGTTGGGTTTTGTT1750.12119785029641532No Hit
GCAAGAGGGCAGATAAAGTTACTAGAGTACTGTATATACATTTTCCTTTCTA1750.12119785029641532No Hit
GCGACAGGGATGTAAGGTAGACATTTAAAATACTTCCATATTTTATGGTTGC1740.12050529115186437No Hit
TCTTATGGGAGGAGAAGTTTGGAATGCACTTAATAGCTGGTTTTTACACCTT1730.11981273200731342No Hit
TCTGACGGGAGTTTCTCACTCTGTTGCTTAGTCTGGGATGCAGTGGCACAAT1730.11981273200731342No Hit
AATTCCGGGGTATTTGTCATGTATAACACAATATTTTGAAGTACAGTCCTGT1720.11912017286276248No Hit
GTCGCAGGGGTTTGTATGATTTTTGTTTTCAATACATGTGATGGGTTACTGT1720.11912017286276248No Hit
CACACGGGGATCGGACTTATTCACGCAGTGGCCAATAATCAAGACAAACTGG1710.11842761371821155No Hit
GTTAAGGGGTAAAAAGAATGAAAAAGTGCTTTTTCCTCAACTTGATAACTGT1700.11773505457366058No Hit
TGGCTCGGGTTGATTGAAGTATATTGTCATATAATAAAGTGCCTGTTATTTA1700.11773505457366058No Hit
GTTGCAGGGATAGTTATTAAAGTGAACTTATCTATCCTTTTGTTTAAACATT1700.11773505457366058No Hit
GTTCTTGGGCGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCCT1700.11773505457366058No Hit
GTAGTAGGGTATAACTCTTACTAACAATGTATAACAATTTGGGAAGAAGTTA1690.11704249542910965No Hit
TACAAAGGGTAACGGCGGGAATTCCTGGGATGAGAGTTTTGGATGCAGTGCT1690.11704249542910965No Hit
TTTGCCGGGGTGGCTTTGCAACCATTTTCATTTGTTTGTTTTCCTTTTATTT1690.11704249542910965No Hit
ACCAGCGGGCTCTAGCAGGGGGAATTAGTCCGAGTGGAGAGAGCGAGCTGAG1680.1163499362845587No Hit
GTATTCGGGGCCTTTTCACAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCT1670.11565737714000776No Hit
CTTTCAGTCTGCTAAACCAAAACCAGCAAAGAAAACAAATCCCTTTCCTCTC1660.1149648179954568No Hit
CTCGCAGGGTCCAACAGTTTAAGGTACTATACCACATGGTAACCTACTGTTT1660.1149648179954568No Hit
GTCATAGGGGCTGTTTTAGAATATGTTCTTATATTACAATAAATGATACCCT1660.1149648179954568No Hit
CTGATTTCCCTAGCAATGATTCTGTTTCCATTGATTCAAATTACTAAAAATT1650.11427225885090586No Hit
CCATAGGGGTAACACTATTTCGGCTGGTCAAGAGGATTTCCCATCTGTCCTG1640.11357969970635493No Hit
ACAGGTGGGGAGTCTTCCAGGTGTGACAATCAGGATATAGAAAAACAAACGT1640.11357969970635493No Hit
TTAGGCGGGGCTGGAGGTGCATTTTAAGTTTTAATAATAAGTGCTAGAATGA1640.11357969970635493No Hit
TATGCCAGGGCCAGGGGATAAACTCTGACTTAGTCTTGGAGTGGCAGATACA1640.11357969970635493No Hit
CGACACGGGATTGTGCCCGGTCGGAGTAAACACAGAATGCTATCGAATCTAT1630.11288714056180399No Hit
ATCTCTGGGAGAATCAGAAGACCTACTGCTGTTTGCACCTTAAAAAACTAAA1630.11288714056180399No Hit
GGACCCTCAATACCTACTTAACCTCATCTATTTCCGTTTGTCCTTTTGCCAG1630.11288714056180399No Hit
TGCAGGGAATCATCTGTGTTTGTTTATTAAACATATGTCACTTTGAAATGCT1620.11219458141725303No Hit
CTTCCTGGGGCGTTAGCAGCCATTGAGGACTTCTTCCTAGAGCATGAAGCTC1620.11219458141725303No Hit
ACTCCAGGGGTTTTATAAATTCATATTTACTGTCCTGATCTAGCCACTATCA1620.11219458141725303No Hit
GCCAATGGGCAGGATATCACACCACGTCTTTTCAAAAACTAAAGAGAATTCA1620.11219458141725303No Hit
TTACACGGGAGGAGATGTGCCCAAGTTTATTCTGCATTGCCATGGGGGAATT1610.11150202227270209No Hit
AGCTATGGGGGCTTACAAGAAGTAACTTTAGGTAGGAGCTGTTTTTGTTTGA1610.11150202227270209No Hit
CACACTGGGATATGTTGTAATTTATGATTCCTGGTTATGTATTTTTGTGGGA1610.11150202227270209No Hit
ACAGCAGGGTTACATGTGAAAGCTAGGCTCTGAGTCGTTATTATGACATCTC1610.11150202227270209No Hit
TTTCGAGGGCAGGGCAATGAGAAAATTAGATGTGTAATATATCTTTCATCAA1610.11150202227270209No Hit
ACTAGAGGGATTTGAAGTCCCTGGCACCTACAGCTTCAGAAAACATTAAACA1600.11080946312815114No Hit
GATAATGGGGCCCGCGGGGGGGACCATGTGCCTGTGTCCCACGAGCAGCCGA1600.11080946312815114No Hit
GACACCGGGTGATGCGGGGCTGTGAATAAAAGGCTTACATTTGTTCCTGCAT1590.1101169039836002No Hit
AAGTTTGGGATTGAGATATGATTCAAATTTCATAAAATCTCATAAAATCCTA1590.1101169039836002No Hit
ACTGCAGGGATCATAGCAGGGATATATGCTAATGTGGAGAGCAGATAAAAAG1580.10942434483904927No Hit
AGCTATGGGAGCTTAGAAATGACAATCTTTGGAGCCTATGACTAATCATGAG1580.10942434483904927No Hit
ACTAAAGGGAGAGAACTGCTGTGTGGTGGTTGTCATTGCTGATAAATACGGT1580.10942434483904927No Hit
TCTACTGGGACCTCAGAGGCAGAGGAACACCACCAGCTCTTCGATCTGATTG1580.10942434483904927No Hit
ATCTGCGGGTGGTGCCTGGGGAAAATATAAGAACTCTGAAATCAACTTCTCA1570.1087317856944983No Hit
ACAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAGATA1570.1087317856944983No Hit
TTGATAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1570.1087317856944983No Hit
CTTAACGGGTTCTCCCTGAAAAATACAAGGGCTGTTGGTGAGAGCAGACTTG1570.1087317856944983No Hit
CCCAGGGCATATAAAGAAATAAAAAGAAGTCTCAGTTGCAGCTATTTGTCAA1570.1087317856944983No Hit
GCAATGGGGGCAGGAAGAGAGACCTGACTGACATGTATATACTCTTGCCCTC1560.10803922654994737No Hit
GTTATAGGGGCGTAGGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAA1560.10803922654994737No Hit
TTCCACGGGGAGGAAATCAATCTCATTTACAATAACATCAAAAAATTGAAAT1560.10803922654994737No Hit
AGCTAGTGGGATTATACTAGAAGAAGAAACCAGATCACTCCACCCCTCATCA1550.10734666740539643No Hit
TGAACAGGGAGCTCACTGCAACCTCCGCCTCCCAGTTCAAGTGATTCTCCTG1550.10734666740539643No Hit
CTAAGCGGGCTGCCATGTCACCCTTCTGATTACAGCCACCTTTTGGGTTTCA1550.10734666740539643No Hit
CGTCAAGGGATTCCTCCTCCCCCATCATAACCATATTCAGCACCCATTTTTA1550.10734666740539643No Hit
GCTATGGGGGGGACATTTTAGAGATTAGGATCCCCAGACAAGTAGTGATGTT1550.10734666740539643No Hit
CAGATAGGGTGGGTTTGTCCAGCTCAGGTAGCTCTAGGAGCACCATCCTGCC1550.10734666740539643No Hit
GCTCTTGGGATTATATATGAATAATATTTTTATTTAACCTTTAAAATTGCAG1540.10665410826084548No Hit
TTTGATGGGCAACAGCCTTTACTGCCATTTTTTTTTAAATGTTTTCACTCAG1540.10665410826084548No Hit
GTTGTAGGGGTAACTGCTGTAGCTGAAGCTACTGGAAGATTTGACTGAGTTG1530.10596154911629452No Hit
CGTTGGGAGGAGTTTCCATGTCTTAGAAGGGCTTCATCATTTTCTTGCTGAA1530.10596154911629452No Hit
TCTGCATGGGGATGGGCAGCTGGTGATGCCATGCCTTTGCTATTGTGAATAG1530.10596154911629452No Hit
AAGCACGGGAAGAATTGTTTAGGTAAAAGGTTTTTAACTACTTAAAAGAGAT1530.10596154911629452No Hit
ATAAGAGGGTGATATCCAGATATGCGTACTTCAGCTAATATTTTATAGCCTT1530.10596154911629452No Hit
TATTGTGGGAGAGTGGAATGGATTGGAACGGGGAGGTGTGAATAGCAGGTCA1530.10596154911629452No Hit
GGTAGTGGGATGTCTATCAGAAGAAACATCTGGTTGAAATTTTGTCATACCA1520.10526898997174358No Hit
ACTGTTGGGATCTAATGGAATACTGTGTGGTCATTTAAATTTATATCATGGA1520.10526898997174358No Hit
TTCCTAGGGAACTTGAAGGACTGTAGGAGGTAGAGGAACATGGAAAATTGAA1520.10526898997174358No Hit
TAACACGGGAACCTTGCTAAACTCTCCTGTTAGACCTGGTAAATCTCTGATA1520.10526898997174358No Hit
AATCAGGGGAGCCTTTGAGAACCTGTATAAGAATACGTAAGTAATCCAGAGC1520.10526898997174358No Hit
CTTTATGGGAAGTGAGGGTACATACAGCATTTCACTGTGTTTTTAGTGTTGA1510.10457643082719265No Hit
ATCCTGGGGTAAACAGTCATGTAACCCCTTTCAACTGTGTGCTTTTCCTAAT1510.10457643082719265No Hit
ATCCGGGGGGGACTCACATGAAGTTGCAAAATTAATAGTGAGAGTCTCATGA1510.10457643082719265No Hit
CGTCTAGGGATAGCACTTTTGTAGCATTTCACACATAGCAACTAATTTAATC1510.10457643082719265No Hit
GCATAAGGGCTTGTTTTCCCCAACATCTGGGATAAATAGAGCTGCTTGTATG1500.10388387168264171No Hit
ACAGTAGGGGCTTCATCTTCTCTCAGGACCCACAGAGCTCAGATGAGGTCAA1500.10388387168264171No Hit
CTCTAAGGGCCCATATAGATATTAAGGAAGGTGTACTTAAAAAATGTTTGGA1500.10388387168264171No Hit
TCGTACGGGGATCTAGGACAGGGGTGAACAGGCATCCTAAAGAATGAGAGAA1500.10388387168264171No Hit
GTCTGAGGGGGAGAGCATTTCTAAAATAAGCGATAACTAGTGATGTTAGTGG1500.10388387168264171No Hit
GTAGTAGGGAAGACACAGGCACACAGAGACTTATTCAAAAGATCATAGAACA1490.10319131253809075No Hit
AATCATGGGGTACTGTTTGGTCACCAGGGGGACCTAGTAGACACCGCGAGTA1490.10319131253809075No Hit
AAAACGGGGTCCCTGGAGTCAGCAGACCTGGCTTCAGTGTTCTTGATGAGTG1490.10319131253809075No Hit
AGTTTTGGGGATGCATCTGCAGCCAGAGTCGCACTAGGAATCACGACGGTGC1480.10249875339353981No Hit
ATGCCCGGGGATAGGGATGCAGCATGATGGATTTTCTACCCTTAATTGTCTT1480.10249875339353981No Hit
TGGGTTGGGTCTGCAAAGCCTCGCTGAGGAAATTGACTTTGAATATCCTTTT1480.10249875339353981No Hit
TATTGCGGGTGTCTTTGGAATACTAATACAAAAATTGTGTTAATGCTACTTT1480.10249875339353981No Hit
TGCTCTGGGGATACTGGCAAGCCCTCTGGGTGTCCAGATCTCATGCAAACAC1480.10249875339353981No Hit
GAGATAGGGCTTACCCCATATTTACTTATTCTGATGTGCTTTTCAAACAAAA1470.10180619424898886No Hit
AGTCAGGGCACAATGGGATACGGACGAGCTGTAGTAAACTATGATTTGTCTG1470.10180619424898886No Hit
TGAGAAGGGCTGAATTTGCCTATTCCAAATATTTTGTATAAGTGGAATCATA1470.10180619424898886No Hit
GTAGAGGGGGTATAATTTACATACAGAAAATCACACAAATTGTGTACAGGTG1470.10180619424898886No Hit
GCGTAGGGGAAGAAAGCCAGACTTTGGAAAAGGATAATTGGAATAAAGGGAG1460.10111363510443792No Hit
TTAATGGGTATTCACATCTCTTTTTAAGACGAAGTATGCAAACATTTGTTTC1460.10111363510443792No Hit
TATTTAGGGGAGACAACCACTGCTCACAGTTTGATGTATATCCTTCCAGAGA1450.10042107595988696No Hit
TTTCTGGGGCCACTCGACTCGGCCTCCCGAAGTGCTGGGATTACAGGCATGA1450.10042107595988696No Hit
GCCTCGGGCAGAGCTAATATTTTTAGAGTAACTGATCTGGATCAATGATCTA1450.10042107595988696No Hit
GATGGTGGGAAGAGAATATAATTGCTCTGCTTTTCCACTAAAACTCCATCTT1450.10042107595988696No Hit
GCCTCAGGGCAGCTAATGTAATACACCATGTCAAAAGAATGAAAACAAAAAC1450.10042107595988696No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)