FastQCFastQC Report
Sat 31 Dec 2022
SRR5846414.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846414.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47773
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGC24745.178657400623783No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCCGTCT20924.379042555418333No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGT6041.264312477759404No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTA4720.9880057773219183No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAT2170.4542314696585938No Hit
AACCTTGGGATCTACAGACACGCATAAAGTAAACACAACTCTTGGAGGGATC2160.45213823707952194No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTCTCTTA2140.4479517719213782No Hit
GACATAGGGCTGGATGTGGACATTTGGAGCGCTTTCAGGCCTATGGTGAAAA2100.43957884160509075No Hit
GAGAATGGGGATGAACTCTCACCTAGTAGAATGGCTAAAATCCAAAACACTG2070.43329914386787516No Hit
GTCCTAGGGATAATTATGAATTTTAGAAAAATGTATGTTCTTAAATATTTTA1950.40818035291901283No Hit
GAACGAGGGTGAAAAGTAAAATGGACTACTTTTGTAACTTACCTGTTTGTTA1890.3956209574445817No Hit
CAACGAGGGTGAAGCATAGTCCTCATGCTTAATAAACTGACTGAAATCGTAG1880.39352772486550985No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTC1860.38934125970736605No Hit
CACATAGGGTCCCCGACTCCCTTCTTTATGGCGTCGCTCCTGTGCTGTGGGC1830.3830615619701505No Hit
CTTACGACGGGTGTCTTTAAAGCATAATATCTCTTAGACTTGACGGTTTGAG1750.3663157013375756No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAACTCGTATGC1750.3663157013375756No Hit
AATACAGGAAGAGTACATGGGTCTCTAACTACCATGAACAAGTTCCAGTATT1690.35375630586314444No Hit
TTTTTTGGGATGGAAGGCCAGTGAAGAAGCAAAGGAAGACATGAAAATTGAC1680.3516630732840726No Hit
AGGAGGGCAGTTTTCCTTAAATAAGTCAAAGATATGTTTACATTGTTATAAT1660.34747660812592884No Hit
GGTAAAGGGATTTGAGGAAGTGCTCAACCACATTTCATTCTTCTGGAGTAGA1660.34747660812592884No Hit
AATTGGGGACAGTTGTGATGGAACACAAGCACTGTATATAAGACAGGTGCTA1650.345383375546857No Hit
CGAACCGGGCTCCTTTCCATCTAGCACAGCATCTTTGTCTCTGTTCTGTCTC1650.345383375546857No Hit
ATAGTGGGTCCCACTTACATTAACTAAATGAGGCATGAGCCACCATGCCTGG1640.34329014296778515No Hit
ATCTTGGGGACTCCAAAGGGGGAAAGACCCTTAGAAAGCCATCAGATCTCCT1640.34329014296778515No Hit
TCACTAGGGTGGTGGAATTTTATTTTCTCAAATGTTTTCAGCATTTAAAATT1560.32654428233521027No Hit
AGACATGGGCTCATTCAAGAGTATGTGCAGGCATGCTTATGATCGTAGGAAC1550.3244510497561384No Hit
GCGTGAGGGGTCAGAAGGCTGGGCAGCCATGGCGTCCTATTTCGATGAACAC1550.3244510497561384No Hit
TGTGACTGGGGAAGTTTTGTTTTAGTTTTGATAATAAATTCTTTGGAACTTT1520.3181713520189228No Hit
GCTGCTGGGGCACATTTAACCCAAAAGAAAAACAAAAGGCATAAAATGAGAT1500.3139848868607791No Hit
GACCATGGGTTATCAGGGAAATGCAAATCAAAACCACACTGAGATATCATCT1480.3097984217026354No Hit
TGCGCTGGGTCTTCTTTCATCTCCTCAAAGGAATGAAGAAATGGTGGTGGGC1450.3035187239654198No Hit
GATCATGGGTGGACTCTGGCGAAGAGCTTCACGGCGTCCTGCCCGGTCAGCG1440.30142549138634794No Hit
CAACATGGGCACTGATGAACAGTTTCTGGTGCAGGATCAGTTCCCAGTGACG1410.29514579364913235No Hit
TGACGACGGGCTCTTAAGGCTGTCGAAGACATGTTGGAAACATTGCAGATCA1380.28886609591191675No Hit
ACGCGGGATGATATTTAAAGAAATATTTACTTTTCAATTTATAAATATACAT1380.28886609591191675No Hit
CAGAGCACAGGAAGCAGCTCAGCCCAATTATAGAACCTGACACCAAGCAAAG1380.28886609591191675No Hit
CACCGTGGGAGATTTTAAGAGAAAATATGAAGACTTAGAAGAGTAGCATGAG1370.2867728633328449No Hit
TTTATTGGGTGGATGGGCTGAAGAAGAGAGACAAAAAGAACAAAACTAAGAA1370.2867728633328449No Hit
CATGCTGGGATCAAAGAACTGAAACTCACCAAATCACTGCATCCAGACAATG1350.2825863981747012No Hit
ATTTCGGGGGAACCCATGTTTGTCACCAAGGAGCTTTCCAGGGCACATGTCA1330.27839993301655747No Hit
GCCAATGGGGGCAGAGGGCCGATCCAGTTCTTTTCGGCTGGAATGGGGAATT1330.27839993301655747No Hit
TCTGCTGGGGTTTACCCCAAGTCACAGTTAGGACGGTAGATGGTGAGATCGC1330.27839993301655747No Hit
AACAGAGGGTGTCTGGGAAACAGGATACACACAGATATACACTTATCTTATG1330.27839993301655747No Hit
GTAATGGGGTACTCCCCAAACTAGAAACAAATTGATAAAAGCAGGTTAATAA1290.27002700270027No Hit
GCATTGGGGATTATTAGGCAGAAATGCACTTGTTTAAATCATAGAAGTAATT1270.26584053754212633No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCC1240.25956083980491074No Hit
TCACTGCGGGGAAATATAAGATATGCCAATACCTTGAGTTTTTCTGTAATTT1240.25956083980491074No Hit
TTCTAAGGGAAGGTGAAATAAAAACAGAAGGACAAGAGCTCATAACCGTAAT1210.25328114206769514No Hit
CAGACAGGGTAGTTGGCCTCTGGTCTACAAAGATTTCATAACTCCTTGGAAA1190.24909467690955142No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGT1160.24281497917233585No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGG1160.24281497917233585No Hit
CATCAGGGGTAGTGACCGAGACTAAGAGGATTGTTTGACTTAATCAATATTA1160.24281497917233585No Hit
CAATACGGGTGTGTCCCAAGCATCTGCTAATCAAATATTATGAGATTACTTG1150.24072174659326398No Hit
TCTTTAGGGGCCCCAAGCCATTCATGAGGGATCTGCCTCCATGACCCAAACT1150.24072174659326398No Hit
TTTGCGGGTAACCTTCCCCAGAAACAACAGTGATTGCGATTGTTTTCTAGAA1130.23653528143512026No Hit
ATTCGGGATTTGAGCATAGAACAAACCTCATAGTTTCTGTTGAGATTTTATT1120.2344420488560484No Hit
TGTGGGGGCTGTGATTCCTGAATCTACAATAATGAGAACTCTACATAGTGGA1120.2344420488560484No Hit
CAGACAGGGATCTCTCTTCGTATTACTTATATACTCCTCTTCTGATTTCAGG1110.23234881627697654No Hit
CGCTACGGGGCCGGCTATGCCCCTGTATTGGATTGCCACACGGCTCACATTG1080.22606911853976097No Hit
TTTTTGGGGGATCTGTATCAGGGCAGGAGCCATGGAGAAGTGGCATCAAGAC1080.22606911853976097No Hit
TAAACAGGGTACCAGCCCAACCAACATGGAGAAACCCCGTCTCTACTAAAAA1070.2239758859606891No Hit
GCAATAGGGGTCTCCTGTTTCTTTTCAAAGAGGAAGAAGAGTTTACAACAGA1070.2239758859606891No Hit
CTAATGGGGCACAATATTTTGTAAAAAGTACCCAGTAGCCCCATTTCATACA1040.21769618822347353No Hit
GAATCTGGGACTTGGGCCCACGCTTCTTAACTGACCCACTCCTCCCGTTGGC1000.2093232579071861No Hit
GATTATGGGAGCAAGACCTCGTCTTTGTTTAGGGGAAAAAAAAGAAATTTAA1000.2093232579071861No Hit
GGTTCAGGGATACAAATCCCGTATGGTTGTGTCATCCTCTCTTAATCATTTT990.20723002532811421No Hit
TGAAGGGGGTTATGAAAGATAGCACAAAGTTTTTCTGAAACTACATTAAAAA970.2030435601699705No Hit
ATGCGCGGCTTTCTTGTTAACGGGTATCTTTTGTTGGTGTGTTTTGCTCTTA960.20095032759089865No Hit
TGCTTAGGGATGGACGCCTGGCAACCAAGTATCATACCGTTCCACTGCCACC940.19676386243275493No Hit
GACAGTGGGAAAGACACCCCCCACAGTTTATGTAGCTTACCTCCTCAAAGCA940.19676386243275493No Hit
GCAGCTGTACTGCTCCCATTGTGCGTTCCTTCCCCTCTTCTCTGTGTTGTCC940.19676386243275493No Hit
GCATCCGGGTGGAGAAGTCAATGGTACCTACTATCAATTTGGCAAACATTAT930.19467062985368302No Hit
GTTGCTGGGAGGTCGCGGCGACATGGCCAAACGTACCAAGAAAGTCGGGATC920.19257739727461118No Hit
AAAATAGGGATAATTTCTATTGGAGAATATCAATAACAAAGAAAAAAGAAAT910.1904841646955393No Hit
AAAGACGGGGGAAAGACAAATCAGTTATAACAAGCAATATTAATTGTGCACT910.1904841646955393No Hit
GCCTGCCTTGGCCTCCCAAAGTGTTAGGATTACAGACGTGAGCCATCACGCC900.18839093211646746No Hit
TGGGGGGTATTGCTGTTGTTGTCATCGTTAACTGTGGGCATGGAATGCGTGA890.18629769953739558No Hit
GATAACGGGATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATT880.18420446695832374No Hit
GTACACGGGCACGACGCTCTTCCGTTCTACATAAGGGATAGGTTTGGTCCTA860.18001800180018002No Hit
CCGTTTGGGGTTCATGGATTCTAAAATGAACCTAAATACGTGGAGAATTTCT860.18001800180018002No Hit
TTTATTGGGATTGTCAAGCTAGCAAAAAAACTCTTGTTTTTTTAATTGGGAG860.18001800180018002No Hit
GATGAGGGGGGGCCAGGCATGGTGTCTCATGCCTGTAATCCCAGCACTTTGG830.17373830406296442No Hit
AAGCGTGGGGTTCATATACTTTTTAATTTTATGTATTTTAAGATATATGTCA810.1695518389048207No Hit
TCGAATGGGACTTTCTCCATACATAACACTTTAGCATTCAAGAAACATAGGA800.16745860632574885No Hit
TTGGGGGTGAATGAGATGATATTGAGAGAGATATGGGAAAGAAAAAAAAAGT790.16536537374667698No Hit
GGCCATGGGGGGCTCAAATTCTCTACCTCTTCAGGGCTAATACTTTTAACTG770.16117890858853326No Hit
GGCTACGGGGGGGACAAGGATCTTAAACCCAAAGTACGAGAAATGTTTCTGT760.1590856760094614No Hit
AATCTAGGGATGGATTGGAAGGTGCTCACATGGCTTCGTTACACACTGTGGA750.15699244343038954No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTC740.1548992108513177No Hit
AGTAGCGGGTACTGACTTGTTGCCATTAAGTGAACTTGACTTCTTATGTGTG740.1548992108513177No Hit
ATAGCTGGGTGCCTAAAGAATTGGCCCTGACATTTGTTAATCAAGTTAACTG730.15280597827224582No Hit
TACTATGGGCCGATCTAAGAAGGGTGATGTAGGGGGAAAAGCAGCAGCCTCG730.15280597827224582No Hit
AACCCTGGGGCTCTTCCGATCTCAAATGGGCTACAGCTCACTTCTATCAGAG720.15071274569317397No Hit
ATTAAGGGTAAAATAAATGGAATGTGCTACGTGATTCCTAGGACTGATTTAA720.15071274569317397No Hit
TTTATTGGGTACGTTCAAAATTTTACTTTTGCTTTAGCAAAAGAAAGTGAAG700.14652628053503025No Hit
TTCACAGGGGGGTCCTCCAGTTTTTGGCACAGATGTTCCGGAGCCCAGGAAG700.14652628053503025No Hit
ATCTGTGGCGTGCCACTGCACTCCAGCCTGAGCAACAGAGCAAGACTCTATC690.14443304795595838No Hit
GCATTAGGGGTTTCTCAAAGTTTTCTATTTAGACCAGTACTCCTTTTCTCTA690.14443304795595838No Hit
AAAACTGCGGCATCCAGCTTCATCTAGGTGGTCATGATTACCTGCATGCTTT690.14443304795595838No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATA660.1381533502187428No Hit
ATATCCGGGGGTTGAATACGGGAACTGCCACGGGGCAAGCTGTCAATGGTGG660.1381533502187428No Hit
TGTTTGGGAGACCGAGACAGGTGGATCATGAGGTCAGGAGATCGAGACCATC630.13187365248152721No Hit
GTTTCTGGGAATTTTTGTTTGTTTGAAGATAGGTTAAATGGCTATATGATCT620.12978041990245537No Hit
ACTGCCGGGTGCTTTATTGCTTAAAAATGCTGACACAGAGACATGAAGTGGG610.1276871873233835No Hit
AACTCTGGGAGCACGAGGAGGGTCGGGACTCGGAGGAGGGGCCAAGGTGTGG610.1276871873233835No Hit
GATGACGGGGGGTCTTGCTCTGTCACCCAGGCTGGAGTGCAGTGGTGCCATC610.1276871873233835No Hit
GGTTATGGGACTGCCTTCTTGGATTTTTTTCTGATCACCTCAGCCAGAAGTG600.12559395474431165No Hit
TCTGGCAGTGGCTACAGGTGTGAGTCAACATGCTCGGCCTGTTACACACACA600.12559395474431165No Hit
CTCGTCGGGATCCCAACGAGCTGCCTCATGTTCTTTATTTTCTAAGGGCAGG590.12350072216523979No Hit
GTTCTGGGGGATCACAGGATCAGAGCTGATGCCCAAAGCTCTATCGACCAGA590.12350072216523979No Hit
GTTTCAGGGTAAATTCCAGAAGGGAACCAAGAACTCTTCCCTTCCCTGGTGA560.1172210244280242No Hit
GATGATGGGATGGGTAAATTATTTAAACTCACTTAATTAAGGAAAATATTTT550.11512779184895235No Hit
TGGTACGGGTCTTTTTATTCAGTAATCATCAGTCTTTTCCAATGTTTGTTTA550.11512779184895235No Hit
TCCTCTAGGCAGTTGGGTTATTCTAGAAGCAGATGTGTTGGCTCTTTCTGAA550.11512779184895235No Hit
CTATCAGGGAACCACGTTATTACTAAAACAATGGAATTAAAGCCCTTAAATG550.11512779184895235No Hit
TCCACCGGTTCCTTTTCCCATTTTCACTGTCCTGTTTTCTCTTTCTTATCAT540.11303455926988049No Hit
AATATTGGGGTGGCCGGGCGTGGTGGCTCACGCCTATAATCCCAGCACTATG530.11094132669080863No Hit
AGTCAAGGGACAAATCTTCATACAGAGTAGGATAAAGAACTATTCAGATATA530.11094132669080863No Hit
CAGCGAGGGAGGGGGCCGGGTGTGGTGGCTCACACCTGTAATCCCAGCACTT530.11094132669080863No Hit
GTTGCTGGGAATTGCTCAGGATGGCTTCAGATGGAAGAAAAACTTGTAACCA510.1067548615326649No Hit
TTTGACGGGCAGTTGAAACCTGTATAACCCTGTTACAAAGCTGTGTTGTTGC510.1067548615326649No Hit
GTAACAGGGTCCACCCAGTTCTTTAGGCAACCACTGATAGCATTTTCTTAAG510.1067548615326649No Hit
GCTTCAGGGAGCTAGAGGCCGCCACGCAAGTGAAGCCAGCAGAGGAGTGATA510.1067548615326649No Hit
TGCTCTGGGGATCTACAGCCGGGCAAGTGTTAATGGTCTCCTACACTTCACA500.10466162895359304No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCG500.10466162895359304No Hit
GCTGCAGGGCATGTCTCTCATCTTCCATGAGGCTGTTTTGGGCTTCATTACG490.10256839637452118No Hit
TACATTGGGTCGCGCCACTGCACTCCAGCCTGGGTGACAGAGCGAGACTCCG490.10256839637452118No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTC490.10256839637452118No Hit
GCCTTTGGGGCTGCCTCCTGCAGGGCACTTTCTACGCTCGGGAGCGGCTGGG480.10047516379544932No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)