FastQCFastQC Report
Sat 31 Dec 2022
SRR5846407.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846407.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107231
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCCGTCT15591.4538706157734238No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGC14601.3615465676903133No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGT3780.3525100017718757No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTA2700.2517928584084826No Hit
GAAAGCGGGTTACAAAAGTGACTGGGTTGGATAACTTCATCAGAATTGATTT1950.18185039773945966No Hit
GCTTAGGGGCATTGGTAAGGCCAACCCTTCACTTTGCTTCTAAGACCCCATA1900.1771875670281915No Hit
ATGACAGGGATAACAGCTTATAGAACTTGAACTACTAAATATGAAAATAAGT1800.1678619056056551No Hit
CGAAAAGGGAGCTGTTTGTTCTAAAATGTTTTGTGATTTGTGATTTTAATAA1760.16413164103664052No Hit
GTCACGGGCAATTATCAGAGAAATATTCAATTATGCTTTTGCTATTGTCTTT1720.16040137646762598No Hit
AATCGAGGGTATGGTCCACTATTGCCAGGAAAAAAGCTTCTAATATTAGTAA1720.16040137646762598No Hit
GTTTGCAGATGACATGATTGTATATCTAGAAAACCCCATTGTCTCAGCCCAA1700.1585362441831187No Hit
AAATTTGGGCATGGAGCTTGAGATCTTCTTAGCCCAGAGAGCAGTGGAGTTG1640.15294084732959684No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTC1600.1492105827605823No Hit
GTCTATGGGGGGAGAATTCCTTGAACCCAGGAGGCGGAGATTGCAGTGAGCC1580.14734545047607503No Hit
TTATGGGGGATGGAAGAAACTTCTAAAACTCTACCGAGTGGTTTTTGTATAT1560.14548031819156773No Hit
GCAGCTGGGCCGATCTCTACAGCGGGGATGACTTGACTGACCCTGCCCCTGC1550.14454775204931408No Hit
CAATGCGGGATTGCCTGCAAGAAAGTTATTGAATTAAGCAGCTATCCAGGTT1530.1426826197648068No Hit
TGTCATGGGAGTGACTGTAGAAATTGAAAGCATTGTTTATTATGTGACATTT1510.14081748748029954No Hit
ACTTCTGGGCCTGTCTGTGATCCAACAAGTCCGATAACATGCTGCTGTATTT1510.14081748748029954No Hit
TAGGACGGGCTACAGAATTGGGAAGCACGTCTACTGCTGGAGAGGTCACATG1500.13988492133804592No Hit
CATCTCGGGAGTAAGAGACAAGACACTACTCTTTGTTCTTTCCTCACAGAAA1500.13988492133804592No Hit
ACTGGAGGGAAGATGAGTTTCCATTTGTTTGTATTCTCTTCAGTTTCTTTCA1490.13895235519579227No Hit
AACCACGGGCACGTCCTCTTTAATGCACTTCAAGTATTCTCTTTCATTTGTA1450.1352220906267777No Hit
GCACAAGGGATTTTTCAAATCTATAAGTACATAGTTCCACCATTTTGGCACA1450.1352220906267777No Hit
TGTGATGGGATCTAATGATAAGATAGGAGTCTCAGTGATTTTTCTTAGTGTT1450.1352220906267777No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTCTCTTA1440.13428952448452405No Hit
ATTCACGGGGCGGAGACTGAGGTTAGAAGGCACAGGTGGCGAGATGAGCCGG1440.13428952448452405No Hit
GATGTTGGGTGAGCTGGGGCATTTTTTAACCGATGTATTTTACACTAATATA1430.13335695834227043No Hit
ATGAGTGGGGATATTGTAATGATGGTATATAAATCACTTTAACTGTAGCATA1430.13335695834227043No Hit
TGTCATTGGGGAAAAACACCATTTGGCTTTTAGGCCTCAAAAATGAGCTTTA1420.13242439220001678No Hit
GAACATGGGTAACAAGTATATAGAAAAAAAATAGATGACCTTTCATATGAGA1410.13149182605776316No Hit
TTTAGAGGGTAGGTCTCTAACTGTTGAACTCATGAAAGAAGATAGTGTATGA1410.13149182605776316No Hit
TTCACAGGGATTTATCTTTAGCCTGAAGAGTTTTCTTTATTATTATGCGAGA1400.1305592599155095No Hit
CCAAAAGGGATGCCTCCTCTGTACAAAATATATGATACAGGAGATTTAAAGA1380.12869412763100224No Hit
TTTGGCTGGGGTAATGCACATCATTTCTATACTATACCAGTAAGTCTTTCAT1380.12869412763100224No Hit
TCGTCTGGGATAATTTTGATGAATATTATTTTTTAAAGATCTGGAAACTGGG1380.12869412763100224No Hit
CAACTTGGGACCCCAAAATAAAAGCCCAAGTCTACCTTTGACTGGTACCACC1380.12869412763100224No Hit
CGTCAAGGGATAAATCTGGTCATTTGATTTTTAGAGTTAGATATTTATCGAC1380.12869412763100224No Hit
AACTCCGGGAGAACAAGAGAAGTCTTAAGCTGCATTTACTCCAATTCCAGAT1370.1277615614887486No Hit
AGATGGGGGGTACTAATGGAATTTGGAAGAGGTGATTACTGTGAGCTGAAAC1360.12682899534649494No Hit
ATTAGCGGGGAACACTTAGTGTCGGACACTGTGCTTGGATGTGGATTAAAAT1360.12682899534649494No Hit
TTAAGCGGGATCTGGGACAGTGTCTCCTCAGAAACAATCGGCCCAGAAGGTT1360.12682899534649494No Hit
AAGGATGGGATCTTTCAGTTGTTGGTTTGTCTTTGTTCCTATTTTTGTATTT1360.12682899534649494No Hit
CTTTAAGGGGTAAGCTGTGCTCCTCTTTTTTTTATTTTTATTTTTTTGGGAG1350.1258964292042413No Hit
CTCGCGGGGATGATAAGTTCACTCAGGCCATTATTGACTGCTGAACTCTCTT1350.1258964292042413No Hit
ATTTAGGGGGATCTTTCTCGGGGTAGCAAAGACAGGAAGACTGAGACAGTAA1350.1258964292042413No Hit
TTACAGGGGATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAG1340.12496386306198769No Hit
GTCTGCTGGGGGAGCAGTGCGCAGTGACCAGTTCTCTGCTGTGAGAGGCCGT1330.12403129691973404No Hit
TCATGGGGGGGTGCACCCATTTGTCCCACCATTCCTCCTTGGGGGCCAACAA1330.12403129691973404No Hit
TGGCTTGGGCAACCGAGCCGAGAGATATTGGTCAAATTATGTAAGCAAGTAT1330.12403129691973404No Hit
TTAGTAGGGAGTGTATGCACATGGTGGGATCAGGGGTAAGAGTGATCTCTCT1330.12403129691973404No Hit
CGTAATGGGGCCTGAGATGTAAGAGAAAAAAGTAGGTGAATATGGTGCTTTG1330.12403129691973404No Hit
AGGTCCGGGACTAGTTGCCATTTTAGTCATAGAATCAATGTGATAACCCATT1320.12309873077748039No Hit
CCACATGGGAACTCCAAAAATTAGAGTATCCCCATAAACATGAAATCAAATT1320.12309873077748039No Hit
GCAGGTGGGATGGACAAACAAGAAATCTTTTAGTGAGAAAAGTTTTTATATT1320.12309873077748039No Hit
GCGTTAGGGGCACGGGGGTCTGAGTAAGGGATGTATAGTATTAGTAAAAGTA1310.12216616463522675No Hit
TATTCTGGGGAATAGAGTACTTGACAGTCAACAAGTTATCTTTCTTCTCAGA1310.12216616463522675No Hit
GGGGAAACTTGTCACATCTTTCTCTGTTTCTTTCATTCCTGTCAGAACAGAT1310.12216616463522675No Hit
GGTGATGGATGTTCTTTACACTTACCACCCTGCCGCCTTATTTCTTTCTACA1300.12123359849297312No Hit
GCTAGGGGGGCTTCTAGTAGACCTTAATCTAGCATAGTTGAACCTCTGAATA1300.12123359849297312No Hit
GTGCTAGGGAGTAAGTAATGTAATCCTTTAATTCTTGCCATTCAGCTGAAGG1290.12030103235071948No Hit
TAAGGAGGGAAGCACAACACGCTATCAACTTCAGCATCATGAGGATTGGCCT1290.12030103235071948No Hit
CGGCCTGGGAAACACTTTAGATTCAGTAAAAAAACTACCTAGAAGAATAAAG1280.11936846620846583No Hit
TTAGCTGGGGTTCCAAGTGTATTCTAAATACATTGATCTTCTTTCTGTTTAT1280.11936846620846583No Hit
TCTCCTGGCTTGAAAGTATAAACCATCACTTAATTCTTATTTTAACTCTCCA1280.11936846620846583No Hit
CAACCTGGGCCGTCTGGGAACAGTACCTCTGCCCCTGATTCCCAATGTGCCA1270.11843590006621219No Hit
TTGTTGGGGATGGTGAAACCATCCCCCGGCTACTTAAAAATACAAAAATTAG1260.11750333392395856No Hit
CGATAAGGGGGAAACAGATTGATGAGCTACTAGGCAAAGTTGTATGTGTAGA1260.11750333392395856No Hit
GACCGGGGGTTTGTTCTCTAGATAGTTACACACATAAAGACACCACTCAAAA1260.11750333392395856No Hit
TGTTAGGGGAAGGTACACGAGTCGATGATGCTAAGGCACTGATAGCGGACAG1250.11657076778170491No Hit
ACTCAAGGGTAGCTGAATACGTTAGAAAATGACAGATGGTAGAGACTTCCAT1250.11657076778170491No Hit
ATGCTTGGGCTAGAACTCCTGGCTCTCTGACTTCAGGGTCTGTGCTATTAAC1250.11657076778170491No Hit
AAGCTAGGGACTGGTAAAGCACCTTCACCTCGTGCTGCCCATGCCTGTGCAA1250.11657076778170491No Hit
GCAAAGGGACAACAGAAACAAATGAAGTGATTTTCCAAAGAACTGTTTTCCT1250.11657076778170491No Hit
CGTCTAGGGCAGGGGCACGTGCTGTGAGCTTCCTGGGAGCCCAGGTTGTGCT1250.11657076778170491No Hit
TCGTCAGGGGCCCATTCCAACCTTAGGACAGGTGCCAGGCTGATTAGGGGCC1240.11563820163945128No Hit
CCAGGTGGGATCTTTTAACCAACTGAACAATACACCAAAAGCAGCCTAGGGA1240.11563820163945128No Hit
TAGTCCGGGACTTCTTAACATAAGGTCCTAGTCGCAAGGGTCTATGGAGGTG1240.11563820163945128No Hit
CAGCACTTTGAGAGGCTGAGGTGGGAGTATCCCTTGAGGCCGTTAGTTTGAG1230.11470563549719763No Hit
GTATCTGGGACATGAAAACAAGACTGGGTAGTTGTAGATTTCTGCTTTTTAA1230.11470563549719763No Hit
TAATGAGGGAAGCAACTTAGCCTTGGAAACAATATGAAGCATAGTACTAATG1230.11470563549719763No Hit
GACGATGGGTACATGGTAGCAGGGTCTTTGTGTGCTGTGGATTTTGTGGCTT1230.11470563549719763No Hit
ACGTGTGGGTACCAGAAGCCATCCGTAAGCCCCTCAGTCACACTTTCCATGT1230.11470563549719763No Hit
CAATTAGGGGATTCAAATAGTTAAATGAAAGAAAATAAACCATAAGACTTGG1230.11470563549719763No Hit
TTTCCAGGGCAGGTAGTTCTGGGGGCGATACTGCCGAAAGGCCCGAACACAT1220.11377306935494401No Hit
TTGCTTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1220.11377306935494401No Hit
CCTAATGGGTATATAATTAAATTTCTTATATTTACATAAGATATGAGTCATT1220.11377306935494401No Hit
ATGATAGGGCTCCTTTGTTTCTTGCAGTTAATGTAAGAATACTTTAAATCTC1220.11377306935494401No Hit
ACTCTAGGGGAATTTTAAGTTGCAAAGGTATCTGAATAATTTTTCATGTGCA1210.11284050321269036No Hit
GCAATGGGGAAGAGATTTTGGGGCAAAAACTCAGAATGGTGTTTACAGTGCT1210.11284050321269036No Hit
CTAGCTGGGGCAGTCTTTCACTTTCTATTCGGCCCCAGCTGGAAGGGAGCAG1210.11284050321269036No Hit
TGCACAGGGGATCTTGAGGTGGGGAATTTTGGGGATTTTTGTGAAATGTGAT1210.11284050321269036No Hit
GCCTATGGGCTCAGCTCTTCCACCCTCTCCAGGGTCCACAGAGCAGGGAGTG1200.11190793707043672No Hit
GAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGAGC1200.11190793707043672No Hit
GCCAGCCCTTTCTGGATTACTGATAGAAAATCATGCAAAACCTCCCAACCTT1200.11190793707043672No Hit
ATGGCTGGGATCTCCATGCTGACATTTGAAAGAAGGTACTAGTATCCCTCTA1200.11190793707043672No Hit
GAACACGGGCTATGCACTGCACAACTGCATAATTGTAAGAGTGTTACCGGTG1200.11190793707043672No Hit
GCCAGCGGGTGTTATATACGATGTGGGAGAGCAGATTGCTGTTCCCCGCAAT1190.11097537092818308No Hit
AACACAGGGAGTTGGGGTAGGTGTTATTATTGTTATTTTTAAATGAGACTTC1190.11097537092818308No Hit
ATTCCTGGGTGGGAATTACTAAACAATCTGTCACGTTTGATAATGTGTAAAT1190.11097537092818308No Hit
CGTTACGGGGTAAAGCTTAAATGTGCTGTTACGTGATTTCCTTTTAAAGTTT1190.11097537092818308No Hit
TGCACAGGGAGATGACTCTTGTTATGTTTTATCAAACAGACTGGTGGCATTT1190.11097537092818308No Hit
GCTCAGGGGCGGAGACCATATGTCAGAGCTAGCATTCTATGAAGCAGTTTTA1180.11004280478592945No Hit
TTGAGGGAACGCTGATTGCCATTTGCATTGTCTACTGAAAAGCTGTGGCAGC1180.11004280478592945No Hit
GCCAAGGGGAGAGCCAAGAAAAACAGCATAAAAACAAAGATGAGACTGTGTG1180.11004280478592945No Hit
GTGTTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1180.11004280478592945No Hit
ATCTAGGGGCGATCTCACAGCGGGGAAGCCACCAAATCTCATGATCAGGAGA1180.11004280478592945No Hit
CATGCTGGGACTTCTCTGCATTCTTTATATTTTGGAGATTGTTTTTCCAATC1180.11004280478592945No Hit
GAGACTGGGTGTTGAGGATTCTGTTCCTGGAATTGTGTGAGCTAGGAATGGA1180.11004280478592945No Hit
TCATCGGGGATGGGCACATATCTCCCACACTTTTTTAGATCAGATATGACAC1170.1091102386436758No Hit
CTTCCTGGGATGGAAAAAGAATATTTGTACATATGTGCTTTTAACAATTCTG1170.1091102386436758No Hit
ATAATAGGGGGCAGGCGAGGCGGTCGCTTCGAGCGCGCTAGTCAGCTCCCTG1170.1091102386436758No Hit
CGCTTGGGGGTGGGGCCGGAGGCTCCGCCCCCATCATGTGCCTGCCACCCCT1170.1091102386436758No Hit
TCTTTTGGGGAATTCCATATGTAGGAGATACTCTTTGCAATGGGGTGTGGCA1170.1091102386436758No Hit
CATCTAGGGGTGATCAGGGTGGAGGGAGACAGGCAAATAGTTATCATGAAGG1160.10817767250142216No Hit
TATCGCGGGATAATTTTGTGATGTGCTACCACTGTTTATTGTCCTACCTGAA1160.10817767250142216No Hit
AGTTAGGGGGGGTAATAATATTTAGAGTATTATTTCAACATTTTCATTTTCA1160.10817767250142216No Hit
AATGGAGGGATCAGCAACTTTATGCTCAATTTTGATACAAACATAGCAATTT1160.10817767250142216No Hit
ATTTAAGGGGAGCCAAAGATGATGGAAGAAGAAACTCTCAAAGCCATGTACT1160.10817767250142216No Hit
AATAATGGGTTAATATGATGTAATGAAAATTGTACATTACCTCAGTGATTTT1150.10724510635916852No Hit
GTGAATGGGTGTGGACTAGTGATGAATAGATATTTTCATAAGAGTTTAAATG1150.10724510635916852No Hit
CTAGCAAAGGGGACCATATCTAGCATTTCTCTGTAACTAATGCCCAGCACAG1150.10724510635916852No Hit
GACCATGGGCTGGTGTTTTGGAGATTTGAGAATACAGCAGGTGAAGAGAATT1150.10724510635916852No Hit
ATATTAGGGCCAATTGCTGTCAATATAAGTGGACATCCCGCTAAAGCATACA1150.10724510635916852No Hit
AGCGCGGGGATTTCTCAAGGTACTTTGATATTGATTAAATCTTACAATAGAT1140.1063125402169149No Hit
AGACTGGGGCTTCCGATCTAAGTTAGGGATCTGTTTACCAGAGATACTAAAT1140.1063125402169149No Hit
TATGCAGGGATGGTTAATCCAATTCTGTGCACCTGAGGTCCATAAATAAAAG1130.10537997407466125No Hit
CAAGTAGGGTTCTCTGAGTCAGCTGACCCCCTCCCCCCAATCCCTCAAACCT1130.10537997407466125No Hit
CTTTAAGGGGACTGTCGCCATGAACAGTGTGGGGGAGGCATGCACGGACATG1130.10537997407466125No Hit
CAGTTAGGGCCAGAACTTAAAGTATAATAAAAATAATAATAAAAAGAGTTGC1130.10537997407466125No Hit
TTGTTTGGGGCAACCAGCACAGCTGGCGCTCTTAGCTCCTGATTGGTTGTGT1130.10537997407466125No Hit
TTTGGCGGGATAGAACATAGATTTAACATTTCCCAATTCAGGACAAATCAAT1130.10537997407466125No Hit
ATGCCAGGGAAGCCTGTTCTTGGAATTTGCTATGGTATGCAGATGATGAATA1120.1044474079324076No Hit
GATCATGGGATACTCCTCAAGAAGAGCAACCCCAAGACACATAATTGTCAGA1120.1044474079324076No Hit
ACTCACGGGGGGAAGAGTGACAGCTACTTCAGCAGGGTTTGAGGCAACATTA1120.1044474079324076No Hit
CTGTTCGGGGACCTGAAGCCAAGAAGAAAATTCTGGTGATGGGAGAAGTGGA1120.1044474079324076No Hit
TTAGCTGGGACTACAGGCGCCCGCCACCACACCTGACTAGTTTTTTGTATTT1110.10351484179015397No Hit
CGCATTGGGCAAAAAATGGGATTTTCTTTTCTATTGCATTGTCAGGTTGCAA1110.10351484179015397No Hit
TAAGATGGGGGGGCTCTTTCCCTTTAAGCTTATGCCAACTCTAGATAGCACT1110.10351484179015397No Hit
ATGCTGGGGTAGGCTGGGGAGTGGAACTTGAGTACAGTTGAATTATTAAATA1110.10351484179015397No Hit
TGACACTAGGTGCCACATAATTAAGATAAAGACAAAAGTGCAAAACCATGTA1110.10351484179015397No Hit
TATATCGGGAAGAGAAAATATGAAGACTTAGAAGAGTAGCATGAGGAAGGAA1110.10351484179015397No Hit
ATAGCCGGGATGTAAAAAGATCACCAGAATAGCAGTGCATTGCTCTTTTAGT1110.10351484179015397No Hit
CATATAGGGTCCACCAAAACGTAGAACTGAAAGCATGTATTTTGGAAGAAAG1100.10258227564790033No Hit
TCTTAAGGGTAGTCTCAGCTTTTTTGAGCACTTTGCATTTTTTAACTTTATT1100.10258227564790033No Hit
GCTGCGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1100.10258227564790033No Hit
GAGGCGGGAGAATCCCTTGAACCCGGAAGGAGCAGGTTGCAGTGAGGAGCAG1090.1016497095056467No Hit
CTTTTAGGGTACACCTTTTGTTGTAGGTCAGCCACTTGCATGATAAGAGCTT1090.1016497095056467No Hit
TAACTAGGGGACCAGGAATGTATTTCGAGGTGTGATATGAGACAAGGATCTG1090.1016497095056467No Hit
CAATGAGGGTGCTCAGTATTGAGACACTGTCAAGATTAAGTTATACCAGCAA1090.1016497095056467No Hit
TTTAATGGGATAATTTATTTTGTTATTTAAGAGCTGTTGTTAAGTGGAGGAA1090.1016497095056467No Hit
GACACTGGGGATCTATAAGGGGGGACTGCATCTGGATAACAGTGGAGATAAT1080.10071714336339305No Hit
GATTTCGGATAGTGTACACATTTAGCTTGTGGTAAATGACTCACAAAACTGA1080.10071714336339305No Hit
CGCCGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1080.10071714336339305No Hit
GGATTCCCCACGGGCTGGATCGAGATGGTCCAGTTATGCCCAGCTCCTTCCT1080.10071714336339305No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)