FastQCFastQC Report
Sat 31 Dec 2022
SRR5846371.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846371.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53064
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGC21524.055480174883161No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCCGTCT19063.591889039650234No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGT4540.8555706316900346No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTA3640.6859641187999397No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGT2430.4579375848032564No Hit
CTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTCTCTTA1830.3448665762098598No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATGCC1760.33167495854063017No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGCTGTC1630.3071762400120609No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAT1450.27325493743404194No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGT1340.25252525252525254No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTAACTCGTATGC1170.22048846675712347No Hit
GAGCAAGGGAAGACATAAGTCAAAATTTATAATTTTGTAAGACTTCAAACTT1120.21106588270767374No Hit
TTTGTCGGGAGTAGAAAAAAATTAACCATAGATTAAACACCACTCTGGTCAT960.18091361374943465No Hit
CAACGCGGGATTTTTATATAATAGCAAATATTTTGGCATATACATTTCAAAT940.17714458012965476No Hit
TGCAAGGGCTCCTAGATTTGCCAGATTTCAATTTAAAGTTTAAAAAGTGCTA930.17526006331976482No Hit
CATCGAGGGCTGTGACATGTTAGTGATGGGCATGACAGCCATGCTGACAGTT910.17149102969998492No Hit
GTTGCAGGGCATCTCCTCCAATGAGGAAACCCACGCACCTTCCTTAGTAATG900.16960651289009498No Hit
TGCATGGGGATGGGAAAAAATTTTTTTGATGTTTCTGTGGGTATGCCTTCAC840.15829941203075532No Hit
GTCTGGGGTTTCTGATGTCTTTCTGACCTCACATCATATTTGGTTCTCCTAC800.15076134479119555No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCGTATG790.14887682798130558No Hit
TATATGGGGACAGTCGTTTGTTATAATGTTGGGTGTGTTAGACCTCAGTGGC760.14322327755163575No Hit
TGGCACGGGTACTCAATATGGAACTACAAAGAATCCAGGTAGAACACAAAAT750.1413387607417458No Hit
ATGACAGGGAGATACTGTGGAGAAGCCATATCTGCAAGTGAGCAGATAAGGC740.13945424393185588No Hit
TTGCCTTATCAGCTTGTGACAACCTTCCCCAGGACAGAAGTCATACAAGGCC740.13945424393185588No Hit
TAAGTCGGGGACAGTCTCTTTTTACTTGATAAAAGTGGTAAATTTTCTTCAA740.13945424393185588No Hit
ACAGCAGGGGAACCTGTTAGCTGCTCCTCTCCGCAGCCAGAAATGCTGCCGG740.13945424393185588No Hit
AACTAGGGGAGATTAAAACACTGAACTGACAATTAACAGCCCAATATCTACA730.13756972712196594No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTC710.13380069350218604No Hit
CCCGTGGGGATTGATGAAGGATCATGCACCTAACACATACTAACTTTTTTAA700.1319161766922961No Hit
CACTAAGGGTCCGCCTTTTCTTCACATCTGTCCCTGGAGGCCGTGAGAAGGA690.13003165988240614No Hit
TTAGGTTGGGGACTGAAGCAAGGAAAAAAAGCAAATCCCCAAACGTGCTAAT680.1281471430725162No Hit
CTTGTACGGGGCTTCCTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAA670.12626262626262627No Hit
TGAACGCGGGGGAAGATTCTTACTGTTAACCAAATTTTGAGCAAGGAGTCTC670.12626262626262627No Hit
TGGGCAGGGATCCCTCATTTCAATTTCCAATTCCATTAAGGGACCCTCTCTT670.12626262626262627No Hit
AGATATGGGCAATATTTTTTAGACAGTTTGATTATTCTTAAAAATTGAAAAC650.12249359264284639No Hit
TTTGATGGGGAAACGATAAGTAAAAGACAAAATATTTTGTGGTGGGAGCAGT650.12249359264284639No Hit
AGCTCAGGGTTATTTCCAGGTTTTGGCTATTATGAGTAAAGCTGCTATGAAT640.12060907583295642No Hit
ATTGTAGGGTGGGGCAGGGCAGCAGGCTTTTGTGTTCTCTAAAAATGTTTTA640.12060907583295642No Hit
ACTACAGGGTATTACCCCCGATCATGCAAAGGCTGTTACCACAATAGTTTAT630.11872455902306649No Hit
CAATCCGGGGATGGAACATAGATTAGTGGTTGCCAGGGGATAGAGGAGTAAT620.11684004221317655No Hit
CTTAGTCTGGGTTCCACTGCAAATGTTTAGATTTCTTTCTTCTTAAGAATAG620.11684004221317655No Hit
GAACGTGGGAGGCACGGTGTCAGCAGGCAACATGGCCGAGAGGCCGGGGCCT620.11684004221317655No Hit
AATCGAGGGGAGCTGGGCTACTGTATACAGTGTACAATGTGTATTTCTTCAA620.11684004221317655No Hit
TTGGCTGGGAATGAACTTGCTTGTGTGTATTTGATGGCTCTAAGGGCTATAA610.1149555254032866No Hit
GTGGCAGGGCATGAATATTTGTGACATTTCTCCCTCATTGTTAACCTCTCTT610.1149555254032866No Hit
ACTTCCGGGTCTCACTGGCAGCTGCATCTGGTGGCGCCAAGGAGCTAAAGAA610.1149555254032866No Hit
GATAATGGGAGAAGTGCTGGGTGGGTGATATCTGCAGGAGAAAAAAATGATG610.1149555254032866No Hit
ACGTCTGGGGGCACAACACAAGTGCTCTGTACCTACAGTAGTTGTTATTGGG610.1149555254032866No Hit
TTTCGTGGGGGGTATAAGTTGGGCCCTTGGCTTCTTGCCCTGCCTACCAGCC600.11307100859339667No Hit
TAAGTCGGGTATAATAACAGGATGTAAAATATTTTCTTGTCAGGTATTTCTC600.11307100859339667No Hit
CTGAACGGGAAGGCACTGCTCCATCCATTGTTTGGTTCTGAACCTGTTGCTG600.11307100859339667No Hit
TACTTGGGGCAAATGTTTGGGAATGGTGCCCTTGGAGGCAACCTCCCATTTT600.11307100859339667No Hit
TGCTATGGGGAATCAGACAGCTGTGTGCAGCTCTCAGCTACACATTTGATAG600.11307100859339667No Hit
ATGCAGGGGGACCGTAGTGTAGAAAGGGAAAGTCTCTTGTGTGTTCTGCAAT590.1111864917835067No Hit
CATTTAGGGAACTGTATTAATTTACTATGAAATGGACATCCTTTTAACTAAA590.1111864917835067No Hit
CGGTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCT590.1111864917835067No Hit
AACGGGGCTCAGGCTCAAGGTAACAGTGTTCTTGTGGTTTGACCAATTGTTT580.10930197497361677No Hit
TGTGCTGGGATTAAGGCGTGAGCCACCGTGCCCGGCCAATACGTACTTTAAA580.10930197497361677No Hit
ACTCATGGGCTCCCGACTTTCCAGAAGAGTTAGAAAAATGATGAAAAAGACC580.10930197497361677No Hit
AGATACGGGATAACTTTTACATATCATGATGAAAATTAGCATTAGGGAACGG580.10930197497361677No Hit
GATGGTGGGCGATTTACTTCTCACAAGGTTATTACCATTCATTTAATTCACA570.10741745816372683No Hit
TTATGTGGGAAAGAATTGTGGCTTATAATTTATCTGAAATTTATTAGCTTAG560.10553294135383687No Hit
GCACTGGGGCTCTTTTTTGGTTCCATATGAATTTTAAAGTAGTTTTTTCCAA560.10553294135383687No Hit
TAGTGAGGGGGACCAAGGTTGCCTCAGGTAACAAACTACAGAAAGTGAAATT560.10553294135383687No Hit
GCATTGGGGTACAGAAGCAACTGAAGGCTCTCAAGTCTTGACAAAAAGAATT560.10553294135383687No Hit
CGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCG560.10553294135383687No Hit
TAAAATAGGGGGGAGGTAAAAATTACAGCACAAAGAAAACTAAGCAGGTGCA550.10364842454394693No Hit
GAAGGGGGGAGTGGTAATAATGTGTGTACTCCTAAATCTTTATGCTGTCCTC550.10364842454394693No Hit
CCGGCTGGGGAGTTTATAAAGTATAATTAATTAAACAAAAAATAAGGATGAG550.10364842454394693No Hit
ACATTAGGGGAAACAATGTCATCAGGGAAAAGCAAGACAGAATTTGGAATTC550.10364842454394693No Hit
TATCGGGGAAATTTATCGAAGTCCAGCTTATCAATGATCTCTTTTATTGATT540.10176390773405698No Hit
AGCTGTCGGGGAACATCAGCTCGGATGTTTGTCTCTCAGCCCTGACACCTGC540.10176390773405698No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGATACGTATCTCG540.10176390773405698No Hit
TGCGTTGGGATTGAAATGAGATGATTTGACATGATGAGATGTAATGATGAAA540.10176390773405698No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)