FastQCFastQC Report
Sat 31 Dec 2022
SRR5846355.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846355.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109572
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATGC20611.8809549885007117No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATGCCGTCT17131.5633556017960792No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGAC6330.5777023327127369No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACACTGTCTCTTATACAC6120.5585368524805607No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACACTGTCTCTTA5850.5338955207534771No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACA3390.3093856094622714No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACAT1830.16701347059467747No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACATCCTGTCTCTTATAC1530.13963421312014018No Hit
ACGCTTGGGGTGCATGTTTTAATCTGAAAGAAACTATCAAGAAGCTCCCAGG1410.12868251013032525No Hit
TGCTAGTGGGCCACTTTTTTATGGGATTGTTTGTGGGGTTTTTTGTACATTT1400.12776986821450736No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACAACTCGTATGC1390.12685722629868945No Hit
TTGCACGGGCTACCCTTTGGCTTGGCTTGACCTCTCCTTGTGAGCTCACCTG1350.12320665863541781No Hit
ATGCGCGGGAAAGATGGTGAGGCAAAGATCAAAAGCGACTATGCACAACTTC1330.12138137480378199No Hit
TACGTTGGGAGATATTTATTAAACTTTCCTTTGCAAAACACAGTTCTTAGAA1300.11864344905632826No Hit
CTAGACGGGGATTTCCCACCGGGGACTGGACACAAACAGGAATGTCTGGAGT1300.11864344905632826No Hit
AAGCGGGAACTGCAAAATATTTGAGAAACAATTCAGTCTCTCAAACATTTTG1290.11773080714051036No Hit
TTCTGAGGGCAGAGACTGGTGGTGGAGCTGCTTATCAAAAACGAAGACATTT1280.11681816522469243No Hit
TAAGTGCGGGAGAATATGAATATTTCAGAGGAATAATTCACAGTGGAGATGG1280.11681816522469243No Hit
GCCAATGGGCGATCTGTTGGCGGGAAGAATTAGAGACAGTAACTCCAAAAAT1280.11681816522469243No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCCTGTCTCT1270.11590552330887453No Hit
TCGTTCGGGGGCTCTTCCGATCTTGAAAAGGGCTGCTTGCTTTCTGGGGACA1260.1149928813930566No Hit
GTAAATGGGAGTATTTATTGATGTATAAAAATAGGATAATGGGGAACTGGAC1260.1149928813930566No Hit
TCAGGTGGGCTACTTGATTAAAAAATATAAAGAACTGAGTGTTTATTTCCCT1250.1140802394772387No Hit
TTTGCTGGGATCTTAGAACTCATTTCAGGTTTGTAATATCCAATTAAGATTT1250.1140802394772387No Hit
CTGCGTAAGGGACCTATGTTAAATACATAGATTCTTTTTTTCTTCCTTTGAT1240.11316759756142081No Hit
GTACCAGGGATTCAGCAGGCTGCTAGTCATGTCTGCTACTTTTCTAGCTTGC1240.11316759756142081No Hit
TCTGCTCGGGGTCATAATTTATTTATTTATTCCTCCATTGAGAGCACATTTA1230.11225495564560288No Hit
TTAATAGGGGGAGGACGGGACAAAAAAATGCATTTGGGAGAAGTCATTTGTT1220.11134231372978498No Hit
GTACCCGGGCAGACGAAACTGACAAATTTATCATCATAATGGAACATTTCAC1210.11042967181396707No Hit
TTACGAGGGCAGAAAGACTTCTTCAAGTACAATCTTTGTTGGATAGAAAAAT1210.11042967181396707No Hit
GTACTAGGGATTCTGTTCATGTATATCCAAGAGCAAAATTGTTTAATGGTTT1200.10951702989814915No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGT1200.10951702989814915No Hit
TCACTAGGGTGAAAGTCACATGACTGAGTAAATTCACCTAGGGAGAATATAT1200.10951702989814915No Hit
TCAGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACTGCGCCTGGCCTA1200.10951702989814915No Hit
TGGAGCGGGATTTTGGAAACTTCTACCAGCTCATTTGATCACAAATGGATGG1190.10860438798233125No Hit
GGCTGCGGGACCGGCACTGCTGCTGCACCTTTAATAAAAGACTTTAATAAGA1180.10769174606651334No Hit
TTCGGCGGGATCTAGCCGTGGGATCACTTGGATATGAACGAGACTGCCAAAC1180.10769174606651334No Hit
CAAGTGGGGAAGGAGGTGAAGAAATGAAACTTGAGACTTGTGACTAATTGTC1180.10769174606651334No Hit
AAAAACGGGCATCCCTCTCTGTAAATTAGTTCCTATGGACTTATTTGGATCT1180.10769174606651334No Hit
TTACAGGGGGGACATGTACTGCCCTGACTAGGTCTAGGACACATGGCAACTC1180.10769174606651334No Hit
TTGACAGGGAGGACTGAAGGCACAGGTGAAGGTGAAGCTGCTCATGTAGTTT1170.10677910415069544No Hit
TACTTTGGGGATCTCTCATAGGGATTGCTGACTAACTAACTACAAATGCATC1150.10495382031905962No Hit
TAATGTGGGATGATAGTAAAGAGCACCTGGCAATGATGGAACGAATATTAGG1150.10495382031905962No Hit
CACTACGGGAAACTTACAGCCTCAGCTCCAGGCTATCTGGCAATAACAAAGA1140.10404117840324172No Hit
TTTCAAGGGCGCTTGGCCATGCCTGGATCTGTTTTGTTTTGGTTTTTGGTTT1140.10404117840324172No Hit
TTAAGCGGGATTTCTCTGACATTTATATAGTTCTTATGTCCATTTCAGTTGA1130.10312853648742379No Hit
GGTATAGGGATCCAATAAAATATAATATTATTATAGATGGGTGATCACAAAT1130.10312853648742379No Hit
TTAATAGGGTTGAATCTTAAACTGTATTTTTCTCTTAGTATTGCTAATGAGT1120.10221589457160589No Hit
ACAGTCGGGGTTTCTGAATGTGAACCCAAAGACCCTACCCCTGGAGAGGAAG1120.10221589457160589No Hit
TTTCGCGGGGATAGAGGAAAATTTATAGCACTAAATGCCCACAAGAGAAAGA1120.10221589457160589No Hit
AAAAAGGGATATCGGGATGACATTGATTTGCAAAACCTCATAGACTTCACCC1110.10130325265578799No Hit
CTTGCGGGGAGATGAAAAATGTGTTTACTGCTTTCAGTCGACCTGATAAAAA1110.10130325265578799No Hit
TAGGTTGGGCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGCGCGCGCC1110.10130325265578799No Hit
CGCATGGGCGATCTAAACACGGGTGAGGGAGAATTCTCATTGGATGATAGAG1110.10130325265578799No Hit
ACGTTGGGGTCCTTAGTAAATTGAAATATCAGCTGAGAGATTATTTGCTGCT1110.10130325265578799No Hit
CTTGATGCGGGTCCATGTTAATGTTTCAGTTAAGTAGACGAAGGAATTCAGA1110.10130325265578799No Hit
GCGACCGGGAGAAATATCCATGACAAAATTACTTACGTATGTTTGTACTTGG1100.10039061073997006No Hit
CTGTCAGGGACAACAAGAAATGGGCTAATGCTATGAATAATAATAACAAACA1100.10039061073997006No Hit
TCAGCTGGGGACGTCTGTGCCCTAACAGAAGCACCGTTCAATAGAACAGCAA1100.10039061073997006No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)