FastQCFastQC Report
Sat 31 Dec 2022
SRR5846350.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846350.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154283
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATGCCGTCT32392.099388785543449No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATGC32042.076703201260022No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACACTGTCTCTTATACAC7850.5088052474997246No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGAC7630.49454573737871316No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACACTGTCTCTTA5380.34870983841382397No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACA5180.3357466473947227No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACAACTCGTATGC2320.15037301582157464No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACAT1930.12509479333432716No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACATCCTGTCTCTTATAC1790.1160205596209563No Hit
TTAACTGGGGCCTGTCATCCAGATGACTGAAAGTAGTTCATGAAAATCAGGA1650.10694632590758542No Hit
CAAACGGGGAGAACCCTCTGGTTTCCTAGTGTGGAGTAGCAGGTAGAAGGAC1620.10500184725472023No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)