FastQCFastQC Report
Sat 31 Dec 2022
SRR5846335.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846335.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161974
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATGC29411.8157235111808068No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACATCTCGTATGCCGTCT28781.776828379863435No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGAC7200.44451578648425055No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACACTGTCTCTTATACAC6850.4229073801968217No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACACTGTCTCTTA5690.35129094792991467No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACA4600.2839961969204934No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACAACTCGTATGC2210.13644165112919357No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATGACAT1980.12224184128316891No Hit
ATAACGGGGATGAAAGCAAAATAAAGAAATTCCCAGATAAACAAAAACAGAA1790.11051156358427895No Hit
GCAACGGGGATGCAGGGTGCTTGTTAAATTATATCTTGATATCGAAAATTAA1730.10680726536357688No Hit
AGTCCGGGAACCAAAATAGTGACAGTGGCAGTACAAATAACTATAAAAGCCT1680.10372035017965846No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAATGACATCCTGTCTCTTATAC1650.10186820106930741No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)