FastQCFastQC Report
Sat 31 Dec 2022
SRR5846315.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846315.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182031
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGCCGTCT10570.5806703253841379RNA PCR Primer, Index 24 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGC7990.4389362251484637No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACACTGTCTCTTATACAC2830.15546802467711543No Hit
GCGCTTGGGTAGAGAAAAGTTGGCTGACAAGAATGTTCTTTATCCAAATTTT2680.14722767001225068No Hit
TACGCCGGGTTAGTGTTCTGTCCTGTTCACCATCTGCTACTGCTTCCCACAC2290.12580274788360224No Hit
GCTCCTGGGCAAACACCTGGCCATTATTAGCTGAGCAAGACAGCAGTGTTTT2140.11756239321873746No Hit
GCGCCAGGGCAGCCGGGGGGGGGTATATAAGGACACAGGAACCAGCTTGAAG2130.11701303624107981No Hit
CTGTTGCGGGATTATTTTAAAATGTTTTCTTAAAATGTTTTACCATATATTT2010.110420752509188No Hit
GTCTAAGGGAACCCAGAATCAAAAGACCACAGATTATGTGATTTCCTTCACC1930.10602589668792678No Hit
GAGAAAGGGAAGACATAGAATGGCTGAATGCATAAAAAGCAAGACTCATTTA1920.10547653971026913No Hit
GTCTGTGGGTACCACTCCTATTCAACATAGTACTGGAAGTCCTAGCCAGAGC1860.10218039784432321No Hit
GCCAAGGGGGGATTGTCTTTATAGTTACTATGAGGATTACATTTAACATCTT1860.10218039784432321No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACACTGTCTCTTA1840.10108168388900793No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)