FastQCFastQC Report
Sat 31 Dec 2022
SRR5846307.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846307.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202903
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGC7970.39279852934653503No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGCCGTCT5320.2621942504546507RNA PCR Primer, Index 24 (95% over 21bp)
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG2550.12567581553747356No Hit
TAGGAGGGCAAGAGTGAGATGTAGAAAGTTGTAAAATAGAAAAAGTGGAGTT2380.11729742783497535No Hit
AACTATGGGGGCTCCCTTCTCGGCAGTGGTACCTGTTCCCGGTGTCCCTGAG2290.11286181081600567No Hit
CGAATCGGGAAGATAATGCAATGTAAGTGTTTGCTACCAAAAATTATTATTC2280.11236896448056462No Hit
GGTCATGGGAATGATATTGGACAAAAAATTTACAGCAAAATTAAGTCAGTTC2130.10497626944894851No Hit
TGTTTGGGGATCCTTAAACTGGCTCCATGAGTGGTCATCATACTGTTAGTCA2060.101526345100861No Hit
AGCTGTGGGGCTTCAGAAGGCAATAGAAGAGAACAACAACTTCAGTAAAATG2030.10004780609453778No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)