FastQCFastQC Report
Tue 3 Jan 2023
SRR5846289.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846289.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70058
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGCCGTCT5830.8321676325330439RNA PCR Primer, Index 24 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGC5340.762225584515687No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACACTGTCTCTTATACAC1370.19555225670159013No Hit
GGCACTGGGTAGACATCTTCATGCCCGTTAGTTCATCGTTTGCCTAGCATGT1300.18556053555625338No Hit
AAATCGGGGACTCGCGCAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGTGGC1250.17842359188101287No Hit
GCGTTTGGGCTGGCTTGCCTTCTCATTTTCTTAATAGTGTCCCTTGGAATAT1240.1769962031459648No Hit
TCAAAAGGGGTTTGAAAATTATGTTCAGGGACTAGTAGGAGAGGGGAGCTAT1120.15986753832538753No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACACTGTCTCTTA1120.15986753832538753No Hit
CGTTTGGGGATTTTAACAAAGTAATATTTTTGTATTGCATTTTTCTATTAAA1100.15701276085529134No Hit
ACGCAGGGGTTTATAAGAATTCTAACCATTGATATTCTTACATTCTTGCCTA1090.15558537212024323No Hit
CGAATGGGGAACCCTCTGTAGCTCACACCGTTTTCTGAACTATAAAATGGAG1060.1513032059150989No Hit
GCTGCGGGGAAAGGCAACAGGCTGTTATGCAGAGCCTGTTGCCTTTCCTCGC1060.1513032059150989No Hit
AGAGGTGGGTTGGATGTTTCCTACAAAACTAAACACTTTTACCATAAGATCC1010.1441662622398584No Hit
TTAATGGGGATTCTGTGACGGAAAGCCTATGTTGACCTCGTCCGGCACTCAA1010.1441662622398584No Hit
GAACTGGGGGATCTAAAGCAGGGATTGGATGGATATGGGAGCTGTGGCCCTC1000.1427388735048103No Hit
CAAATGGGGACAAGAATGTTGTCTTCTAACTCTGACATTATACCTTGTTTGT1000.1427388735048103No Hit
GACGTGGGATGACTTTAGATTGTTCTGTTTTCTTCCGTAGTGTATAATCAAT990.14131148476976219No Hit
AGCTCTGGGGATTTCCTGGTTTCCTCCTGCATTCTGCTTTACATTCTTGAAG980.1398840960347141No Hit
AAAGAAGGGCATTCAAGCTGAATTTTGCTCCAGTCTCCTTCAGTTTCCATCT980.1398840960347141No Hit
TTTCGAGGGCGTGCCCTTGGGGCTCCGTGTCCTGCTGTCTTTCCGTCCGCTG980.1398840960347141No Hit
CAAAAAGGGAGGTGGTTCTCCGTGTGTTTATATACACAGACGATCCCCGAAT980.1398840960347141No Hit
AGGCATGGGACATGTGTTTTTCTGGGGAAAATGGGTCCATGATACTCTAAGG980.1398840960347141No Hit
TTGCGTAGGGGAAAATATTTTGCATTATTGTTAGCTAAAATCAACCTCTCCT970.138456707299666No Hit
ACGCTAGGGGGCTCAGAGTGTGGTCAGGCGGCTCGGACTGAGCAGGACTTTC970.138456707299666No Hit
AATGAGGGGACGTTACAGATTCGAAGAGAGAGAGGACGACCGTTCTTCCCTC970.138456707299666No Hit
GGTAAAGGGGGATATGACACGCGATATCGCAGTAGAGATGGGGTTTCACCAT960.1370293185646179No Hit
TAAACATGGGTGAGGGATTTCCTGGGACATGAGACTTTCAGAGATAATACCG960.1370293185646179No Hit
CAATTTGGCAGTTCATTAAACCAGGCCTCAGCTTCAGTGCCTCATCTTGCCA960.1370293185646179No Hit
TAACCAGGGCATCAAGTGCATAGCTTTTAGCTTTTAACTGAATTTTTCTGGG960.1370293185646179No Hit
AGAACAGGGAGTTTCTTAAAACGTATTTTCTGATGTGGCTTTACATTTCAAA950.13560192982956978No Hit
CAAATAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA950.13560192982956978No Hit
ATAATGGGGAGAAATGTCTGGGTGTTTTATTTGCAGAGAACAAGATGTGTCT950.13560192982956978No Hit
ACGTACGGGGACTCGTGTCTCGCTACCAGCTCCCCGCTGCCCTGCGCTCGGC950.13560192982956978No Hit
TTGTGCGGGAAGGGGACTGCTTTGAAGGGCCATTGTTCATTTGGAATGATAA940.13417454109452168No Hit
AATGCTGGGGAGTCAGTCTGACGGTCAGTGGATCGGTGGGTTTATCTCAAGG940.13417454109452168No Hit
CTGATCGGGAGGTGCTTCATATACATTTCCTTTAACTCTGTGACATTCCTCC940.13417454109452168No Hit
CTAACTGGGAGGCACTCACCAAATTCTTATTGAATGAATGAGTGAGTCAATG940.13417454109452168No Hit
TCTAATGGGGAACACACATTGGAGAGAAACACTATGAATGTAAGGAATGCGG940.13417454109452168No Hit
TCACTAGGGATGTCAGTTTGCAGTTATGCAGAATCCAAAGTAAATGTCCTGC940.13417454109452168No Hit
TAAGTGGGGCCGTTAATTTGTTGAATTAAATGCCTTTATAAAAATATTTACA940.13417454109452168No Hit
GTTGTTGGGGTCACACTGGCCTTGCCCTTTCCTATCCTTAAGCTATGCCACC930.13274715235947357No Hit
CACTTAGGGAAATATGTGAAATCTCTGACCAATACTGTGACAGCTCTATTAT930.13274715235947357No Hit
GGTGCCGGGTCCTTATCAAAGAAGGGGCAAGCTCTTTTGCCTTTTAGGCCAG930.13274715235947357No Hit
GTTCCAGGGATGTCCACCGAAGTGGTCGAACTGCTCGAGCTACCAATGAAGG930.13274715235947357No Hit
AAGCCAAGGGTGGAGTGACATGCCCCAGATTATAAAATAGGTGGGCAGTGTG930.13274715235947357No Hit
GCAATTGGGGATCTTAATTGGGGTGATCTTTGTAGATTGGTTATTTCTGTAC930.13274715235947357No Hit
AAGTAAGGGAGGGCTCTGCGGTTTCTGGCATCTCCAAGCTTCCAGGTGCCAC930.13274715235947357No Hit
AGCCATGGGACCATCTAGGGTTGTGTAAGTGCACACTGTGGAGCGCACAAAA930.13274715235947357No Hit
GAAGTCGGGGGGCAGACCAGGGCCAGGTAGACGGGGCTGTTTGGTGCCTGAA920.13131976362442546No Hit
TACTAGGGGAGCTTTTGAAGAAAGGCCAGTGATCCAGCAAAGGGGGAAAAAT920.13131976362442546No Hit
TAAGCTGGGTGTTGAGTGAATTAATGAATTAAGAGTGGCTGGCAGAGTAGGC920.13131976362442546No Hit
CACATTGGGATTGGTCCTTCCATTATTTCTTTAGGAAAGTTGTACTTTTAGA920.13131976362442546No Hit
ACTTTGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC920.13131976362442546No Hit
AGACTTGGGAGTAGAAACTGTGAACTCTGTGACTTTGACTCAGGACACAGAA910.12989237488937738No Hit
GCGCGAAGGGAGTCAAAAGAACAGGAATTCGGAAAACTCTTCAGTAAATATC910.12989237488937738No Hit
AGACTAGGGATAACAGGAGTGCCACTTCATGGTGCGAAGTGAACACTGTAGT910.12989237488937738No Hit
GTTTATGGGGACAGTATTAAAGAAATGTATGGAATACAAAGATGAGTTATTC900.12846498615432927No Hit
CATCCAGGGGCTTTGGCAGGGAAATATTACCAATGATAAGTAACGATAGATA900.12846498615432927No Hit
ACGCTTGGGAGGAGGGTACTTGTGTGATGTGGTTTATTTATTTTTTTGTTTC900.12846498615432927No Hit
TTTTAAGGGCAGCTGGCATGTAATGAGATTTTTATTTGCATGGCTTTTTTTT900.12846498615432927No Hit
ATTAAGGGAAGGAGAAGCTTTTTCTTCTTCCTATTTGTTTATTCATTTTGTT900.12846498615432927No Hit
TGCACTGGGAAGCAAGAGGAAAAAAACAACACAATCAAGGGACAAACTACAT890.12703759741928117No Hit
GTTACTGGGACCGGGAAACCACTGATCAATCAAGGAATTGACGTGGTCCGTG890.12703759741928117No Hit
GTCATGGGGTGGTTAGTGAGAAGTAAAATAATGGAAAGTAAAATAATAAAAG890.12703759741928117No Hit
CACTAGGGGAATGCTGCAAATTATCAGTATTTATAAAGTAACTGATTTTGCA890.12703759741928117No Hit
GATTGGGATAGTGCCAACACGTGGCGCGCCTTTGATGGCCCGGGTGCACACC880.12561020868423306No Hit
AAGATAGGGATGGGTTCAGTGGAAATGTTTGGAACTCTGAAGGATTTAGACA880.12561020868423306No Hit
CACGTTGGGAGGCCCGCTCAATGCGGACAGGGTACGAGATGCTCACGCTCTC880.12561020868423306No Hit
ATCGTCGGGTTGCTAGACCCAGCACCACTTATAAATTTAGTACACTGTTCAG880.12561020868423306No Hit
GTTGCAGGGACATGGATGAGGCTAGAAACCATCATTCTTAGCAAACTAACAA880.12561020868423306No Hit
CGGCCGGAGGGTGTGTCTTTGTACTTGGTTCATTGCCCTTCACCTGGTAGAG870.12418281994918497No Hit
AGACCTGGGATCTCAAAATGAGATTTGGGCAGGGACAAATATCCAAACTATA860.12275543121413686No Hit
GTCAACGGGGCATTTTGTGGGGTACATCATTTCTATAATTGTATAATGTATT860.12275543121413686No Hit
TTAAAGGGGGCCTCAAGCCCTGCCTCTTGTCACAGTGAGGGTTCTGAGAATA860.12275543121413686No Hit
AGATTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA860.12275543121413686No Hit
CTCGCTTCGGGAAGTGCTGGGATTACAGGTGTGATCCACTGCACCCGGCCGG860.12275543121413686No Hit
TACAGAGGGGGACTCTCTCTGTAAGGAGGGAAGGGGGAGAAGGTAGAATTCT860.12275543121413686No Hit
GCACAGGGTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGA860.12275543121413686No Hit
CTGGCTGGGAAGAGGTATAGTTATAAAACAAGACTACAGAAAAAGTTGTGGC860.12275543121413686No Hit
TAGTCTGGGAGTTTTAAGGAGATTCATAAACAGGTGTAATAAGATGTGATTA860.12275543121413686No Hit
TCAGTTGGGTGGCATAGCTTTAGTAATGTATGGGAGGATGGAAGAGGCTGAT850.12132804247908875No Hit
CTGCCGGGGAAAGTGAATGGATGACTGTTCATGAGAAAAAGCAGGGTAAAAA850.12132804247908875No Hit
TAAGCGGGGAACCAGTAGTCATGTAGACACAGCCATACTATGAGCTTTCTTC850.12132804247908875No Hit
ATCGCAGGGAGATTAAGTCACAAAAATACAACTGTTTAGATAAATATTCAAG850.12132804247908875No Hit
GAACATGGGAATGTAAAGGGTTATAGCAGGTTTATAAGAATCTTATGGTTAA850.12132804247908875No Hit
TTATTGGGATTATTGAAAGTTGAAACTTAACATGTATGAACAAAAACCAATA840.11990065374404064No Hit
TTTCTAGGGTTTCTGGAACATTTATCAAGGACAATTTGCTAGAAGAAATGGA840.11990065374404064No Hit
TTCCGGGGTCATTTCTAATTGTGTTTATTTGAATCTTCTCTCTTTTCTTTTT830.11847326500899255No Hit
GACCGGGGGGACGCTCTTCTGATCTCAAAGTGGGGATGACATTATGCTAAGT830.11847326500899255No Hit
ATTGTCGGGTAAGAATTGGAGCAATGTTGGCCTATACGCCCCTTGATGAGAA830.11847326500899255No Hit
GACAAAGGGGAGAGAAGCCGCCACTCGCAACATGGAGAAGATCCAGGTCATC830.11847326500899255No Hit
GGGTTCCGGGTTATTTGAAAATCCAGTCCTTTATTAATAGAATGGAGGAAAG820.11704587627394444No Hit
TTTAGGGGGCAGTTTCTTCGTTGGCACATAAATGTAGTAATACCTCCTCAGG820.11704587627394444No Hit
TTACGTGGGAACCTAGAAGGTTTAAATATATCTCGAGAACTATTTTATACAT820.11704587627394444No Hit
CATGAGGGGCAGAGAATTCTCAAGAGCAAGGGCTGCTCCCAGAATTCTTCCA820.11704587627394444No Hit
CTGCAGGGGGGCCCTTTTCTCCAGCAACTGCAGAGGCATTTCAGGAGTTGGA820.11704587627394444No Hit
TTGCTAGGGCATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCA820.11704587627394444No Hit
ATGCAGGGGACCCATTTGAGTGATATTGCACAACAAACACAGATACCTACAG810.11561848753889634No Hit
GAGCATGGGGAAGATAGATCATAACTTATCCAATCTGAATAACAGAGATTGT810.11561848753889634No Hit
TAGTCAGGGCTGGATTAGATGCATGAATTTTGAAAATACAACTTACTAGCAC810.11561848753889634No Hit
TAACAGGGGATAATTGGCCATTGGTGGTCAACTCAGCCTGCAGCCCCTCTTT810.11561848753889634No Hit
CTCGCCGGGAGATGAGACATGTTGTAACCCTGTGGCCTGTTTTCTTATTTTG800.11419109880384823No Hit
ACATTAGGGAACCTCAACAAAGCACCTAAAAACAGTATTTTCTTATGTAAGA800.11419109880384823No Hit
ATTATTGGGGAGGCTCGCTGCGCTCCCTGTTGCCCAGCGCGGGCCCGTTGAG800.11419109880384823No Hit
GTTTCAGGGGCTCACTGTAGCCTCAATTTCCTAGGCTCAAGGGATCCTCCTG800.11419109880384823No Hit
TTGTACGGGCAAGCCAGTTTCCAGAAGGGCTGGGGTGAGATCCTGACCTGTG800.11419109880384823No Hit
CTTATGGGGAGTGGTAGAGCTGATAAATTCTGTGAGAGAATTTGTGTTTGAA800.11419109880384823No Hit
GCATTTGGGCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCT790.11276371006880015No Hit
GAGCTGGGGACGGAGGCCACAGGATCCCAGCCCGGCCTTTGTGGGACTGAGG790.11276371006880015No Hit
AACCTTGGGAGACACACAGGTTAATTATACATATTTTTAAATTTTGCAAAGG790.11276371006880015No Hit
ACTTTTGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATG780.11133632133375204No Hit
GACAGTGGGGTTAATTCATGAACAGGATATAACAAATGTAAATATATATGCA780.11133632133375204No Hit
TGCCGGGGCTCTGCCTTTGGTGTTTGTACATGTTAAGAATTGACCAGTGAAG770.10990893259870393No Hit
ATTTATGGGTGTTCTGGCAATTCTTGTTTCTTGATTTTTCTATATAAATTTT770.10990893259870393No Hit
GTGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG770.10990893259870393No Hit
ATCGTTGGGGGACTAGAAAGCAGGTTGTTTTGTTTTGTTTTGTTTCCAAAGC770.10990893259870393No Hit
ACTTTAGGGAGAGTCTCACTGCTGGGGAGGAGAGGACTGGGCCTGATGGAAG770.10990893259870393No Hit
GCATGGGCACAAATATATGGTATACATAGCATATAGAAAGAAAACTAGACCA770.10990893259870393No Hit
ACGCGGGAAAATATTAAACCTGGAAAATGTAGAAAGAAGAAATTATGAAGTA770.10990893259870393No Hit
GAGAAAGGGCTGGTGATTCAAGTGAATTTTAAAGCATGTTTATGCTGTTGCA770.10990893259870393No Hit
CTGCTTGGGACTAATGCAAAGATTACAGGGCCTTATTCCAAAGCAGTAATCC770.10990893259870393No Hit
AACCGGGATGGCATACTAAGAATTTAACTTGTATAAATTACCTCTGAATAGT770.10990893259870393No Hit
GTTTTAGGGCTTCTTTCCCATCTGGCGGCCGCGGCTCCTGTCCAGACCCTGA770.10990893259870393No Hit
TTCATTGGGTCAATAAGTGGGTGGAGAATAACACAAACAATCTGGTGAAAGA770.10990893259870393No Hit
ATCATCGGGCGCTGCCTTTTTAAAATAGTGTGGTATTTCAAATATTGCTAGA770.10990893259870393No Hit
TTGTCTGGGAGGAAGCGGAAGCGGAGAGGCGGTCGGGATCCGCTGCGCGAGC760.10848154386365583No Hit
TGTTATGGGATGGTCTCTGGGATTTACTCAACAAGTCTCACTTTCCCCATGT760.10848154386365583No Hit
ATTACAGGGTATCTGTTTTTCCTGTTGTCAAATAAAATAAAATTCCAAACCA760.10848154386365583No Hit
TTCATGGGAGAATTTCATATCTAGCCAAACTAAGCTTCATAAGCAAAGGAGA760.10848154386365583No Hit
AAACAAGGGATGTTGAATAGGAGTGGTGAGAGAGGGCATCCCTGTCTTGTGC760.10848154386365583No Hit
TTCGCTGGGGACCAGCATACAAGTATAGAGACCTAGGAGGTGGTCTTGTGGT760.10848154386365583No Hit
TTCATGGGCAAGATTGAATCAGGAAGAAATTGAAACCTGAACAGACCAATAT760.10848154386365583No Hit
AGACTAGGGCTGGGGTACTCTAGAAATGGAAGGAAATTACTTCACAGCAACA760.10848154386365583No Hit
TATGTTGGGCAAAATTGGAGAAATTACCATTAAGGGTCTCTTCCTGACAATT760.10848154386365583No Hit
AGTATGGGGAATGGATAGAATTTTTTCATTCTATATATGATTACGGTTCACT760.10848154386365583No Hit
TGTTAAGGGATTGTGATTCTGAAGCTTTCTCTTTAGGTCAGATATTGTTATA760.10848154386365583No Hit
TGTCTGGGGTTAGAAACTTACTGGAAATGCTGACACGGATTTTATGCCTTTT760.10848154386365583No Hit
TAGATGGGGGGAATTACAATTATGCCAAACAACAGTGAAAGCACATCTAGTA750.10705415512860773No Hit
AGATTAGGGCACGGTAAACTTCATTCATAGATCCTCCCTTATTTAACATCAG750.10705415512860773No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG750.10705415512860773No Hit
CCGAGAAGGGGATGGAGAGGGATTCCTAACCCATCTGGATATGACATCTGGG750.10705415512860773No Hit
GGTTTTGGGAGAGGATAAACCGTTAGCAAATGAATGTAATAATTACTCATTT750.10705415512860773No Hit
ATACTAGGGCTGATTGCATTGGTCAGTATCTCCAATACAGTGTGAAATATAG740.10562676639355963No Hit
CGCTTAGGGGAGTGATAGAACTCCTAGAAAATTTGCAAAGAAATGTTACATA740.10562676639355963No Hit
CATTGTGGGAATCACTTTCTAAAGAATCTTTATAAGAGATAGTTTACACCAC740.10562676639355963No Hit
TCAAATGGGAACAGCAAATAACCATTGGTTAATCTGGACTTATTTTTGGACT740.10562676639355963No Hit
AGCTGGGGGGTTCTCTACACTTACTGTAATACTGATTATTCTTACATATCAA740.10562676639355963No Hit
TAAGCAGGGATACCCTGCTGGATTCATGGATGTCATCAGCATCGAGAAGACA730.10419937765851152No Hit
AATACTGGGAGCTATTGTTTCTTCAGTCCAAATGGTCATACTTTGGGTGATG730.10419937765851152No Hit
TACTATGGGCAAGAAGCTCGGTGCACAGGTTTTCTCTGGTTCTTACAGGAAC730.10419937765851152No Hit
CATAAAGGGATCGGCACGTATGATGGTGGTTTGGTCAGATGGATAATACAGC720.10277198892346341No Hit
CCATTTCTCAAGATTCTGCGAAGGTGCTTTTCCTACCTCTTCTGACACCCTG720.10277198892346341No Hit
GGTAGTGGGCCAATGGGAAAGGCAACAAGTAACATAAAGGCATTTGTCTTCA720.10277198892346341No Hit
AAATAGAGGGTATCATCAGCAAACAGTGACAGTTTGACTTCCTCTATACCGA720.10277198892346341No Hit
TGCTTAGGGGTATTCCCAGATTCTTTCCTGAATAGATTTGAATTTAATGTCA710.1013446001884153No Hit
TGCTCGCGGGCGTTACACAATTTTAGAATTTTGTCAAAGAATAAATGCTTTA710.1013446001884153No Hit
GTTTCAGGGTGGTGCAGATATGAGAAGGTGATTACAGGTAGACCAATGGGAG710.1013446001884153No Hit
ACTTTCGGGTAAGATGAAAAGGGTTGAGATGATGAGATGAAATGAGATGAAA710.1013446001884153No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCTC253.2422744E-546.33405311
TGTGACG253.2422744E-546.33405314
CACACAT253.2422744E-546.33405313
CTTTAGC206.0507207E-446.33405310
TATACCA206.0507207E-446.33405312
AGTAGTC206.0507207E-446.33405314
TTTCCGG206.0507207E-446.33405316
TTTTCCG206.0507207E-446.33405315
TAGTCAT206.0507207E-446.33405316
ACACATT253.2422744E-546.33405314
TTTAGCC206.0507207E-446.33405311
TGTAGAG206.0507207E-446.33405314
GTGACGG253.2422744E-546.33405315
ATGTCCA206.0507207E-446.33405310
GATCAGC206.0507207E-446.33405316
AGCCGCC206.0507207E-446.33405316
TACCAGA206.0507207E-446.33405314
TGACGGA253.2422744E-546.33405316
GTAGTCA206.0507207E-446.33405315
ACCAGTA206.0507207E-446.33405311
Produced by FastQC (version 0.11.5)