FastQCFastQC Report
Sat 31 Dec 2022
SRR5846272.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846272.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130292
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGCCGTCT7580.581770177754582RNA PCR Primer, Index 24 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGC6320.4850643170724219No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACACTGTCTCTTATACAC2150.16501396862432074No Hit
ACGTGGGGGATGACAGCTTTGTGGAGAAATGAAAACATTTGGAAAATAGTGT1910.1465938046848617No Hit
TTCGTCGGGACAAGCAGTGAATGACTGCATTTGAAATATAAAACACAAAGTC1880.14429128419242931No Hit
TTATGAGGGATGGCAAGAAAATACTGAATGCACTGCCATAATGTAAATATGA1810.13891873637675375No Hit
TCTCAGGGGGGAGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTA1780.13661621588432138No Hit
TGCCTTGGGGGCACACATCAAAAGACAGCAAGGCAGCAAAGAAGAAGAAAAA1770.13584870905351057No Hit
CTAATGGGCCCTAGAGAAGGATTTAGATCTGTTGGCCTCTGTTCCATCCCCT1700.13047616123783504No Hit
TCCACAGGGGGAGGAGGAGGGTCGGGGACGGAAGCGCAGAGCACGGACCCCG1670.12817364074540263No Hit
CAGCCAGGGTAATTTATAGATTCAAGGCCATCCCCTTAAGCTACCAATGACC1670.12817364074540263No Hit
TTTGCCGGTCCAGCAGCAGAGTAGACAACGCTCCCGACCAGCAGAACTCCCA1620.12433610659134867No Hit
CGCGTGGGCGTACTGAAACATCGTCTTTACCCACTTCTCAGTTTATAATGGG1610.12356859976053788No Hit
GTTTAAGGGTGCTAGCCACATGTGGCTATTTAAATTTAGTTAAAATGGAATA1580.12126607926810548No Hit
GCTACTGGGAGTTACAGACATCCATACTCACTTTTTAAAATAACTTTTTAAA1570.12049857243729468No Hit
AGCTTCGGGCAGCAATTATTTTTATTGTCTTCAGAACTTCTTTTTGCAAGGA1570.12049857243729468No Hit
TGTCACGGGGTTTGGGTCACTTCATTATGTTGACAAATTCCTTATTTGATAT1560.1197310656064839No Hit
TCGCTAGGGAGCTTAGGTGATAAAATGAATGTACACCTTCCCACTTATTAAC1550.11896355877567309No Hit
ACGTCGGGTATTTATGCCACCAGTAATTTTATAAGAAATCTGAATAGTTCTC1550.11896355877567309No Hit
TAGCTAGGGTACCGCACAGGCATGCTGCAGTGAATTTAACTGATCCTCTGTC1550.11896355877567309No Hit
TACTATGGGAAGAAAATACTGTAAACAACTTTACATACATGATAGTTTAAGA1540.11819605194486231No Hit
AATACCGGGGTCTGGAAAACCTGTGTGATCGGCACAAGTACAGCTGCCCACC1540.11819605194486231No Hit
ATCCTGGGTTAGTATTCAATAGTATTTGTATTAAACAATAAGTACATATATA1510.11589353145242992No Hit
GATGCTGGCCACAACCTAGTTTTTTGTAACTAAATGTAGTAGATTTTTAAAT1480.11359101095999756No Hit
CAAACGGGGTCAGCTTTACACTTCACAGGAGATTGATGGCATTGAGGAAGAT1470.11282350412918675No Hit
CCTAGCGGGAGGATATTTTCATTTATGATGGGTTTATCAGGAAGTAACCCCA1460.11205599729837594No Hit
TCAACGGGGGGGATTCAAATTCAGTCCACAATGCAGTTGATTTGTATGTCTC1460.11205599729837594No Hit
TCCACTGGGGGTATTTGGTTAAAGGTCTCCCACAAGACTGGTATTCTCTTTG1460.11205599729837594No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACACTGTCTCTTA1460.11205599729837594No Hit
GCGCCTGGGGGTTTTAAAAAATGCATTTTCATCAAGCAGATGGTGCACACCT1450.11128849046756516No Hit
TCGTCCAGGGATCACAGCAGTCCTACTTCTCCTATCTCTCCCAGTCCTAGCT1440.11052098363675436No Hit
TTGCCAGGGCTGAAAACCGAAAGTCAATTCTAATTATTCATCTTACATATTT1440.11052098363675436No Hit
TACGCTGGGGTACACTGTTTGCATTTTGTTTTCATTTATATCTTGGGAGATC1420.10898596997513277No Hit
GCTGCTGGGCTTTGAGCCAGGGTGGGGAATGACTTTCTTTCCTGGAATACAC1420.10898596997513277No Hit
CGATTAGGGGAAGAAATGTGACTTTTGTAAGATTATTTCTCTTGTTATTGTC1420.10898596997513277No Hit
CACGCAAGGGACGTTGAGATTCCTCGGCGGGTAGGCAGCGGTGCTGGGCATG1420.10898596997513277No Hit
GATACAGGGTACCCAACCACATGCCACGTAATATTTCAGCCATTCAGAGGAA1410.10821846314432199No Hit
TGCAAAGGGAGCAAATTTAAGTGTTTCTGTCTCTGCTCCATTCCTTTCAACT1400.10745095631351119No Hit
GCTTTGAGGGGAAAATGTATTAGCTAACTCTACCACAAAGAATTAGCAGGTC1400.10745095631351119No Hit
CATTAAGGGACCAGAATACCCACACTGGCTGAGGGCATGGGGTAAAGGTTAG1400.10745095631351119No Hit
TTACATGGGAGATGCCTTTCCTAAGAACTCAAAGTTCTCTTTTCAGCGTGCT1390.10668344948270041No Hit
ATCAGTGGGGAGCAAGAAAGTTTTGGAAATTGTGTTGCTTTTAAGAAATAGA1370.10514843582107881No Hit
AAAGCAGGGAACTCTTTTGCAAAATGAGAACAATTAGATCATATTTTCATGG1360.10438092899026802No Hit
CGTCCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1360.10438092899026802No Hit
CGTACTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1360.10438092899026802No Hit
TCAGCTTGGGCAACAGAGCAAGACCCTGTCTCCGAAACAAATAAAAAATACT1360.10438092899026802No Hit
TAGTTTGGGAGGAAAAGATAAAAGGTTTCTAAAACATGACGGAGGTTGAGAT1350.10361342215945721No Hit
ACACTTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1330.10207840849783562No Hit
TGAAATGGGATCAAAATCATAGCTGGCTCTTTTCCAGGAAATAAATAAGCTG1330.10207840849783562No Hit
TGCTGGGGGCTCTGGAAAAAGTACTCATCCTTTCCTGAATTCAGAGCCCTTC1330.10207840849783562No Hit
TTAAAGGGGACCCTCTGATCCCTTTAATCTTTGTTTCGTTTTTGGTTTTTTA1320.10131090166702483No Hit
GACTCTGGGGAGATAGAGTCTGGCTCTGCTGCCCAGGCTGGAATGCAGTGAC1320.10131090166702483No Hit
TAAACCGGGATCCCTCTCCCATCGTGGGGGTGGCTCCGTGACCTTCCTGCCA1310.10054339483621404No Hit
GCACCTGGAATTATTTCTGCCCTTTCAAAACAGGATTCTGATGAAGGGAGTG1310.10054339483621404No Hit
CTAGCCCGGGCAACAGAGTGAGACTGTCTCCAAAAATAAAATATAAAATTTA1310.10054339483621404No Hit
ATGTTAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1310.10054339483621404No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)