FastQCFastQC Report
Sat 31 Dec 2022
SRR5846251.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846251.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75504
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGC15041.9919474464929012No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGCCGTCT14871.9694320830684466No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGT1930.2556155965246874No Hit
ATAGAAGGGGCATGTGAATGTGCACAAGTGCCCTAGATACTGTTCTTCCCCC1570.20793600339054885No Hit
GTGCGTGGGCAAGCCAACAAGGGCATAAGGCAATTTGCCTCTATAAATGTAG1400.1854206399660945No Hit
CACTTTGGGAGTAAAAGATAGATACCTGGATTCTTGTTTGCATTAGGTTTCC1390.18409620682347955No Hit
AAAAGCGGGCCAGTGATCAATTTTAGAATCAAAAAAGGTGGGACAAACATTA1380.18277177368086459No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTA1360.18012290739563466No Hit
AATTGGGGGGCACATTCCTTGTTAAAAGCACGTCTAATTAGTTAAAGAAAGA1330.1761496079677898No Hit
CTACAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1320.17482517482517482No Hit
CAGCAAGGGATGCGAGGGCAGTGAAAATAGTGTTTGACTCCATTGACAACCT1300.17217630853994492No Hit
ACAGTCGGGGAGTGCTTAAGCTAAATGTCATCACAAAAGAGTAAAAAAATTT1280.169527442254715No Hit
GCGTGGGAGGTAAACACTAGGGTGAACTTTGTGTTAATCTTTCTCATCTTTC1210.16025641025641024No Hit
CTGTTTGGGTAGCATCTGGGAATGTTACTGATTTTCATATTTCGACTGTATA1170.15495867768595042No Hit
ACTAAGGGGATGCATTACCACATTCAAACACAAATCCATTGTAAAATTAGAC1170.15495867768595042No Hit
GCCTATGGGGTTAAAGACTACTACTCTTATTTTCTTCCACCTTTCTGAACTA1160.15363424454333544No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGCC1120.14833651197287562No Hit
AGTGCTGGGTCCAGTTGCAGGTAATTTTTTGTCTGCTAGTAGTGTTCTAGAT1110.14701207883026066No Hit
AACACAGGGTCTCTTCGGGGGGTGAATATAGATTTGATTCAACTTTTAGTTA1080.14303877940241577No Hit
GCCCGTGGGCAAAAGAAAAATAATATAGTTAAAGTAAGGGGAAAAGTAAAAA1060.14038991311718585No Hit
GTGGTTGGGTAAGTGTTAGAAGTAATAGTTTCTTTTAAAGAGTAACTTCCCT1060.14038991311718585No Hit
GACCAGGGGTGTCCTACTGTCCGTGGCAGAGCTGTACTGGCGATCTTCCTCC1040.13774104683195593No Hit
TTTCTGGGCTCGGGGAGGGATGGTATTCTACCAAATAATTAAAAACTATACT1030.13641661368934097No Hit
GTAGCGGGAGAGCAGGAGAAAAGGACCAGCGCAGCCTAGACATAAACACTGC1010.13376774740411104No Hit
TGTTGTGGGGGAAGTGGCCCTTGCAGGCAAGAGTGCTGGAGGGCGGCAGCGG1010.13376774740411104No Hit
TTTTATGGGAGTAGGTTTGGGCTATAAACAAATTCATTTTCAAAGGTTATCT1000.13244331426149608No Hit
AAGAAAGGGGAATAAGCTTTTATCAAATAGATAATTGATGCAATTTAGGATT990.13111888111888112No Hit
CACAAGGGGCATGTTAAGAATACCCCTGTGGTAACTCAGGAGTCCAGTGGAG990.13111888111888112No Hit
TACATAGGGAGTTGCTATTGGACTAATTTAATTGTGAAAAAAATTATAATGT980.12979444797626616No Hit
TCGACAGGGAATGAAAATTCTCTTACAAATATTTTATTTTCTCTTCTCAATT980.12979444797626616No Hit
CATTTTGGGAACCTGTGTTATTACAGAAAACAATACTAATTGCCTATGAGTA980.12979444797626616No Hit
AACATCGGGTAAAACAAGGGACAGTACTTTGAAGAAGAAATAGGTCTCTATT970.1284700148336512No Hit
GCACGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGAC960.12714558169103624No Hit
AACATGGGATTTCCTAATATGGTAACTAACTCTGCTTTCTAGTATGATTCAG950.12582114854842127No Hit
AAAAGCGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA940.12449671540580633No Hit
GCGCTGGGATTGTAAAAAGAGTAAGAGAGGTTTGGTTTGTGGTGGTTTGCTT940.12449671540580633No Hit
CGCTTAGGGGGGCAAAGGCATGACATCTATCTGCAATTATAGTATCATATAG930.12317228226319135No Hit
ACTACGGGGATGCTTCTTTTATTTAAGCACACAGATTCATGACACAAGTACT920.12184784912057639No Hit
CAGAAAAAAAAAGAAAAGAAAAGAAAGAAAGCGGCTTTGATGTTTTATATTA920.12184784912057639No Hit
GAGCACCGGGATTCTCAGGAAATTCAACCAGAAATGACACTTTGCCCTCCCC910.12052341597796143No Hit
TGCAGCTGGGAAGGCTGATTCTCTGCTTGTCCCTGGCCTAGAAACCACACTT910.12052341597796143No Hit
AGCACAGGGATTCCCAGAGGAAAATTTATAATCTTAATTGCTTTTATTAAAG900.11919898283534647No Hit
TCGAGGGCCGATCTAATAGGGGGCAAGGAGAAATAGATCCACAATTACAGAG890.11787454969273152No Hit
CGCAAAGGGAGGCTCACTGAAACTTCTGTCTCCCAGGCTCGAGCGGTTCTCC890.11787454969273152No Hit
ATAACAGGGACTAGTTCTGAACTTTGGACATATTAGTTTCTATGAGCCTCAG890.11787454969273152No Hit
CTTTGAGGGCACGTGAGGACGCACACTGGAGAGAAACCCTGTCAGTGTAAAC880.11655011655011654No Hit
ATCGCGGGGGAAATTTCCAATTAGCACAGGAAAATTCTAGAGCCATGTTATG880.11655011655011654No Hit
TCTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA870.11522568340750158No Hit
TACATAGGGAGACCATTCATGTTTGAAGGGCATAGTGGATACACAATAGTTT870.11522568340750158No Hit
GTCTTGGGGGTAGCTTTGTGCTTACAAATATAAGTGAGTCTGAATTGAAAAT870.11522568340750158No Hit
TTTGCGGGGTGGTTTACATCTAAATTAAGGGCAGGAGCTGTCTTTCAGACAT870.11522568340750158No Hit
CACAACGGGTTATTTTTTATGAATAAAAATAAAAATACATTAGTATTTTGAG860.11390125026488664No Hit
ACTCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG860.11390125026488664No Hit
TAAATGGGGGAGCCATTGTGCCCGGCCAACAAAACTACTAAAAATAACTATA860.11390125026488664No Hit
GTTTCTGGGAATCATATTTGGAGGAAGTTACTAATAGTAGGTTTAATTTGTA860.11390125026488664No Hit
CACAATGGGACAAAGGAAGCATTGAAGACCGTCTTAAAGAATTTCTGGCGCT860.11390125026488664No Hit
ACATCCATGCTCTGAAATATTTAACCACCTTGAAGGGAGGAATGTAGCACAG850.11257681712227166No Hit
ATGCATGGGATTTCCTCTGATGGTACATAGATTTCTCTCTCACTAAGAGGGT850.11257681712227166No Hit
GTATCTGGGATTTCTTCAAGGGGTGATGAAAACCTCGGAAGTTTTTAATTTG850.11257681712227166No Hit
TTGCGAGGGGGAAGGAAATATGATCCTCCTGAGACTAACTTTGTATGTTAAG840.11125238397965671No Hit
GTATCCGGGAGAGCTATTGAAAGTAAACTGATGGTTGCCGAGGGCTGGGGGA840.11125238397965671No Hit
GGGGTTGGGGGTGGTAGGCATAGGGGCAAGCCATGTAAGCTGAGGATTGGGG840.11125238397965671No Hit
ACGCTCGGGAGAGAGCGGGGTTAGGGCTTCATTGCCTTGTGTCCTCTGCTCC840.11125238397965671No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCCTGTCTCTTATAC840.11125238397965671No Hit
CACAGAGGGTGCACCATGAATTTGTCAAATGGATATTTATAGGAATATATTC840.11125238397965671No Hit
ACCTATGGGATGACTCAACAACCAGTTAGATCCCAAGTGCCTGGACCTCCAC830.10992795083704175No Hit
GGGCCATGGAGGAGACTCCGTCTCAAACAAACAAAAACCTTCATCTTAAAAT830.10992795083704175No Hit
CAATTGGGGTCAGAACCTAGGCCCACTGGATGAGCGTGGAGCCGCCCAGCTG830.10992795083704175No Hit
TCAACCGGGTATGATTGTCTTTATCCAAACAGTTTTATTTTAAAATAGACCT830.10992795083704175No Hit
CGCGAAGGGTCTGACGCTGGGCGATCTAGCGTAGGGAATTCTGTCACTCGAA830.10992795083704175No Hit
GAAAGGGGGCATCGCTGGGTCAATAGTACTTGCCGCAGTACTCTTAAAACTA830.10992795083704175No Hit
AGGTATGGGCTGTGACACAGAGGCCAGGGTAGAGGTAGTACAGGTTGTAGGT830.10992795083704175No Hit
ATTAAAGGGGAGAAGACATCGGACTGGCAAACGGTAAGTCGTGTTACCTTAC830.10992795083704175No Hit
GTACAGGGGCACAAAGTGAACATCATTTTATGCTATCTCTTCCAACAAGCAT830.10992795083704175No Hit
TCGCAGGGGAGCGCGGAGGGAGGCGCGCGCGGGAGCGAACACCCTCCCGGAT830.10992795083704175No Hit
CGCAATGGGAACTAAAATAGTGTTGTCATTGTGGGAATTTGGTTAAATATTT830.10992795083704175No Hit
GCTAAAGGGCGACGTCTTCCGATCTTGTCTAGGGGTGATTTTCCTACAAGGA820.10860351769442678No Hit
TCTTGAGGGATATACTAAAATATAAAGCTATATTCAGCTGAAATTCAGTTTT820.10860351769442678No Hit
CCTTGAGGGGGAAGCCTAGAGATCACAGCACGCACTTTCTGGGAGCCCTACA820.10860351769442678No Hit
TGTTTAGGGGGGCCACAGTTAAGATATTAATACATTTCTTATTGGAGGTTTT820.10860351769442678No Hit
CACATGGGGATTCATGTGATAGCTAATATATTTGGTTTTAAATGCATCTTAT820.10860351769442678No Hit
AACCGGGAGAAACACAAAGATCAAGGCTCAGCAGCTGATAGACTCAGCAACA820.10860351769442678No Hit
GGCGCGGGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTAC810.10727908455181183No Hit
CTAAAGTAGGGCTTGTATCTAATCCAGGTGACACCAGCATTTAGAGACTATG810.10727908455181183No Hit
TGAGGAGGGAGCCCCACAGAGCAGGTGTTGCCAATGCTGCGGTTCGTGCAGA810.10727908455181183No Hit
GTCAGTGGATGAACAATAAATAACAACTGTTCTGAGTTAGCTTGAAAGCTCC800.10595465140919687No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTAACTCGTATGC800.10595465140919687No Hit
TTTTACGGGGATACAGGAAATTTATTTGCTCCTTCATCCCTCATGAACAGTC800.10595465140919687No Hit
TGCAGTGGGATATGCATTATATGCAACTAAAAGTGTGTATTTCTTATGCAGT800.10595465140919687No Hit
GATCTAGGGGCAACAGGCAGCTGACTTGATTTCTCGAACTGCTGTTGTCATG800.10595465140919687No Hit
AACCTGGGGGACCCAGACTGTGTGCACAGATGGCTGAAACAGTCAGACCTAC800.10595465140919687No Hit
GAAATTTTCCCAGCACACCCATTCCTTCCTTCCTCACCTCAGCTCTTGTCTT800.10595465140919687No Hit
GATTAAGGGGCATGTGTAGAAGGCAATTTATCAAACCTATTGCACTGCCATG800.10595465140919687No Hit
ACAACAGGGGTCTACGTTATAGGGCATCTGTCTCATGAGGCCTCATCATTGT790.10463021826658189No Hit
ACTAGCGGGGAAAGCAGTGAAAACAGACTTCATTTTCAATAAGTTGGGAGAT790.10463021826658189No Hit
AAGTATGGGGTTTTGCCAGAAGTTTTTGGATGCAGTTATAACTAGCATTCTG790.10463021826658189No Hit
CACAGTGGGGGGCTATGTATGTCAAAAAGGATGTTCCTTTGTGGCAAAAACA780.10330578512396695No Hit
TATCTTGGGGAGTGGTTGAGTTTGTAATACCTTGTTTAAAGAATAATGGCTT780.10330578512396695No Hit
GTACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG780.10330578512396695No Hit
GCATTTGGGATCTTTGGGGTAGTTTTTGTTTCATAATGAATTCTAATTTTAT780.10330578512396695No Hit
TCAACTGGGGTAAACGATTTGGAAATAGGATGTTTTCAACGTTCTTTTGTCT770.10198135198135197No Hit
ATCAAAGGGCAGATTTTTCAAAATTGGACTGTTATGTTTTCAGTATTGTTAT770.10198135198135197No Hit
CGCTGGGGTATTTTGACAGTTTAGGCTTCATTAATAGAACTATTCTGATTAT770.10198135198135197No Hit
ATTATCGGGATATGTAATGTGATTTATAAGGATTGCAACATTAAATGTTTGT770.10198135198135197No Hit
TGGATAGGGAAGATATCCAACATATCTTTTATATTGGGCTTATGTCACTTAG770.10198135198135197No Hit
AAACACGGGTATTCAAAGTGATGAGAGATAATACTGTGAGCCAAGAATTCTG760.10065691883873702No Hit
TCGACAGGGCAGTTAGTGGAGTAAGTCATGAAACCCTTAGGAAGAAAAACAA760.10065691883873702No Hit
ACGAGAGGGGACACAGTTAAATTACTCCTCTCTACTCCTAAAGGGACTTGAG760.10065691883873702No Hit
GAACACGGGGGTGAAAGAGGACCTAATTCTTGAATTATCTGCTTGAACTGTG760.10065691883873702No Hit
AAGACAGGATTCTTATGTTGTCATTATTATTTACATATAATTTCAGTAAATG760.10065691883873702No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)