FastQCFastQC Report
Sat 31 Dec 2022
SRR5846249.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846249.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73824
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGCCGTCT14751.9979952319029042No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGC14311.9383940182054618No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGT2330.31561551798872994No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTA1660.22485912440398784No Hit
CAGCAAGGGATGCGAGGGCAGTGAAAATAGTGTTTGACTCCATTGACAACCT1300.17609449501517122No Hit
AAAAGCGGGCCAGTGATCAATTTTAGAATCAAAAAAGGTGGGACAAACATTA1270.1720307758994365No Hit
CTACAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1200.1625487646293888No Hit
CAGAAAAAAAAAGAAAAGAAAAGAAAGAAAGCGGCTTTGATGTTTTATATTA1190.16119419159081058No Hit
TACATAGGGAGTTGCTATTGGACTAATTTAATTGTGAAAAAAATTATAATGT1180.15983961855223233No Hit
GTGCGTGGGCAAGCCAACAAGGGCATAAGGCAATTTGCCTCTATAAATGTAG1180.15983961855223233No Hit
AGTGCTGGGTCCAGTTGCAGGTAATTTTTTGTCTGCTAGTAGTGTTCTAGAT1150.1557758994364976No Hit
GCGTGGGAGGTAAACACTAGGGTGAACTTTGTGTTAATCTTTCTCATCTTTC1150.1557758994364976No Hit
AACCTGGGGGACCCAGACTGTGTGCACAGATGGCTGAAACAGTCAGACCTAC1150.1557758994364976No Hit
CTGTTTGGGTAGCATCTGGGAATGTTACTGATTTTCATATTTCGACTGTATA1140.1544213263979194No Hit
TTTCTGGGCTCGGGGAGGGATGGTATTCTACCAAATAATTAAAAACTATACT1140.1544213263979194No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGCC1140.1544213263979194No Hit
ACATCCATGCTCTGAAATATTTAACCACCTTGAAGGGAGGAATGTAGCACAG1110.15035760728218467No Hit
ATAGAAGGGGCATGTGAATGTGCACAAGTGCCCTAGATACTGTTCTTCCCCC1110.15035760728218467No Hit
TAAATGGGGGAGCCATTGTGCCCGGCCAACAAAACTACTAAAAATAACTATA1100.14900303424360642No Hit
AACATCGGGTAAAACAAGGGACAGTACTTTGAAGAAGAAATAGGTCTCTATT1080.14629388816644995No Hit
CATTTTGGGAACCTGTGTTATTACAGAAAACAATACTAATTGCCTATGAGTA1080.14629388816644995No Hit
AACACAGGGTCTCTTCGGGGGGTGAATATAGATTTGATTCAACTTTTAGTTA1050.1422301690507152No Hit
CACATGGGGATTCATGTGATAGCTAATATATTTGGTTTTAAATGCATCTTAT1030.13952102297355873No Hit
AAACACGGGACAGAACATTTTCCTTCATCTGTTCTTTTGTTCGTTTACAGCC1030.13952102297355873No Hit
AAGAAAGGGGAATAAGCTTTTATCAAATAGATAATTGATGCAATTTAGGATT1020.13816644993498048No Hit
AAATTAGGGAGCCTAGCCGTTTACTCAATCCTCTGATCAGGGTGAGCATCAA1020.13816644993498048No Hit
TGAGGAGGGAGCCCCACAGAGCAGGTGTTGCCAATGCTGCGGTTCGTGCAGA1010.13681187689640226No Hit
AACATGGGATTTCCTAATATGGTAACTAACTCTGCTTTCTAGTATGATTCAG1010.13681187689640226No Hit
GAAAACGGGCTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAG1000.135457303857824No Hit
CGCTTAGGGGGGCAAAGGCATGACATCTATCTGCAATTATAGTATCATATAG1000.135457303857824No Hit
CACAAGGGGCATGTTAAGAATACCCCTGTGGTAACTCAGGAGTCCAGTGGAG990.13410273081924579No Hit
GACCAGGGGTGTCCTACTGTCCGTGGCAGAGCTGTACTGGCGATCTTCCTCC980.13274815778066754No Hit
TCGACAGGGAATGAAAATTCTCTTACAAATATTTTATTTTCTCTTCTCAATT980.13274815778066754No Hit
TACATAGGGAGACCATTCATGTTTGAAGGGCATAGTGGATACACAATAGTTT970.13139358474208931No Hit
TATGCGGGGCACTTTGTGTGTCTCCTCTTAATTGAAGGGAGGGCTGAGGGAT970.13139358474208931No Hit
ATTATCGGGATATGTAATGTGATTTATAAGGATTGCAACATTAAATGTTTGT960.13003901170351106No Hit
ACAGTCGGGGAGTGCTTAAGCTAAATGTCATCACAAAAGAGTAAAAAAATTT930.12597529258777634No Hit
GTTTCTGGGAATCATATTTGGAGGAAGTTACTAATAGTAGGTTTAATTTGTA930.12597529258777634No Hit
TATATGGGTGGGAAAAGTAGCTTAGCCTTTACACCTACTTTATATCCTGGAG930.12597529258777634No Hit
CACTTTGGGAGTAAAAGATAGATACCTGGATTCTTGTTTGCATTAGGTTTCC920.1246207195491981No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTAT920.1246207195491981No Hit
ACTAAGGGGATGCATTACCACATTCAAACACAAATCCATTGTAAAATTAGAC910.12326614651061985No Hit
GCCACAGGGGGCTCTGAATCTCTCAAAATGTGCCGTCTTTGGTTCCTCATGG900.12191157347204162No Hit
GCCTATGGGGTTAAAGACTACTACTCTTATTTTCTTCCACCTTTCTGAACTA890.12055700043346337No Hit
GTATCTGGGATTTCTTCAAGGGGTGATGAAAACCTCGGAAGTTTTTAATTTG890.12055700043346337No Hit
GATGCAGGGAGTTGTGCAAAGGCTTTAGTCTGTTACGTATACTATAGACACA890.12055700043346337No Hit
GCCCGTGGGCAAAAGAAAAATAATATAGTTAAAGTAAGGGGAAAAGTAAAAA880.11920242739488512No Hit
ATCAAAGGGCAGATTTTTCAAAATTGGACTGTTATGTTTTCAGTATTGTTAT880.11920242739488512No Hit
CGCGAAGGGTCTGACGCTGGGCGATCTAGCGTAGGGAATTCTGTCACTCGAA870.11784785435630689No Hit
GATTAAGGGGCATGTGTAGAAGGCAATTTATCAAACCTATTGCACTGCCATG870.11784785435630689No Hit
AAGTATGGGGTTTTGCCAGAAGTTTTTGGATGCAGTTATAACTAGCATTCTG870.11784785435630689No Hit
CGCTGGGGTATTTTGACAGTTTAGGCTTCATTAATAGAACTATTCTGATTAT860.11649328131772867No Hit
AATTGGGGGGCACATTCCTTGTTAAAAGCACGTCTAATTAGTTAAAGAAAGA850.11513870827915042No Hit
TATACTGGGTGCCCTAAGGAAGTAGAATCGGATCTACTAAGATTCTTATTTT850.11513870827915042No Hit
GTCTTGGGGGTAGCTTTGTGCTTACAAATATAAGTGAGTCTGAATTGAAAAT850.11513870827915042No Hit
GAAAATGGGTGGGCATCTTCTCTCTTCTGAATGCAAAATTTATTTGGTAACT850.11513870827915042No Hit
GGCTCGGGTAGACACAATAAAAAATGATAAAGGGGATATCACCACTGATCCC840.11378413524057217No Hit
GTGGTTGGGTAAGTGTTAGAAGTAATAGTTTCTTTTAAAGAGTAACTTCCCT840.11378413524057217No Hit
GAATCAGGGCTTTATATAGATGTTAGAACAAAGTTTCTCATGCTGTTTCTGT840.11378413524057217No Hit
GCACGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGAC840.11378413524057217No Hit
TTTTATGGGAGTAGGTTTGGGCTATAAACAAATTCATTTTCAAAGGTTATCT830.11242956220199392No Hit
GTATCCGGGAGAGCTATTGAAAGTAAACTGATGGTTGCCGAGGGCTGGGGGA830.11242956220199392No Hit
ATTGAGGGTCTTCTTGCATGTCTCTGAAGATATGAATTACACTTACTGTAAT830.11242956220199392No Hit
TTAGTTGGGCATATCTGAAAAATTATAAGTATCGTCAGGTGCAGTGGCTCAT830.11242956220199392No Hit
CGTAGTGGGGGAGGAAGATAGCAAATAGGAAACATATTGGTGAGGAAGACTG830.11242956220199392No Hit
GCTTGAGGGAGAGCTCAGCAGAGATAATTTCTATTTCTTGGAAGTAATAAAC830.11242956220199392No Hit
TTAGGTGGGCACCCTTGTCTCTCTACACATTTTGCCTTTGGGGATCTGGTTG820.1110749891634157No Hit
ACGCTCGGGAGAGAGCGGGGTTAGGGCTTCATTGCCTTGTGTCCTCTGCTCC820.1110749891634157No Hit
TGGCAGGGGCTTAAGAGGCAGAGAATGCTGAACTGAAAACTAATTAGGCAAG820.1110749891634157No Hit
AAGTATGGGCTGGCTTCTAATCCCAGCTCTTCACCTGACCAGCTGTGTGGCC810.10972041612483745No Hit
TCAACCGGGTATGATTGTCTTTATCCAAACAGTTTTATTTTAAAATAGACCT810.10972041612483745No Hit
CTTTAAGGGGTATGAAAGCTTGACTTTGGTATTGGGAAAGGTGATTTAGAGG810.10972041612483745No Hit
TTGAATGGGCCTTCGTCACCCTAACAAAATAATTATGAAAATATAGATTTTC810.10972041612483745No Hit
CGCAATGGGAACTAAAATAGTGTTGTCATTGTGGGAATTTGGTTAAATATTT810.10972041612483745No Hit
TCGAGGGCCGATCTAATAGGGGGCAAGGAGAAATAGATCCACAATTACAGAG800.10836584308625921No Hit
ACTACGGGGATGCTTCTTTTATTTAAGCACACAGATTCATGACACAAGTACT800.10836584308625921No Hit
AATAGAGGGACTTATGCTGTATATTTGCAGTACTACAAAGAAAATTTTTACC800.10836584308625921No Hit
GTACACGGGGGGGTCCCAAACATGGTATGCAGAAATGTGATGGTTACAGGTC800.10836584308625921No Hit
AAGCATGGGGGTGAGAAGATGATCTGTATTTGGTATGGAGCAGGTGGGTGGC800.10836584308625921No Hit
TATCGCGGGTATTGAAGGACTGTATAGGTTTCTTATTATTGGTTTCTAATGC790.10701127004768096No Hit
ATCATTGGGCTTCTAGAAAATCACAGAAGAAAATCTTTATAACCTTGGGTTA790.10701127004768096No Hit
GAAAGGGGGCATCGCTGGGTCAATAGTACTTGCCGCAGTACTCTTAAAACTA790.10701127004768096No Hit
TCTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA790.10701127004768096No Hit
GCTTCAGGGCAAATTGTATAGCCAGGAAAGGAAATGTATAACCTTTGTAGTT790.10701127004768096No Hit
TGATGCGGGAATATTTGAAAATATACTACACATAAAAAAATTTTGAGAACCA780.10565669700910274No Hit
CCTTGAGGGGGAAGCCTAGAGATCACAGCACGCACTTTCTGGGAGCCCTACA780.10565669700910274No Hit
TATCAATGGGCGATCTTAATTAGGGATCTACAAACCAAAACAAGAACTTTTA780.10565669700910274No Hit
ATCGCGGGGGAAATTTCCAATTAGCACAGGAAAATTCTAGAGCCATGTTATG780.10565669700910274No Hit
GATTATGGGCATGGCGGGATGACAACTCTAGTGCAATATTAGAGTTTCATTT780.10565669700910274No Hit
TCGGCAGGGCCTTTCCGTCTGGCGGCAGCCATCAGGTAAGCCAAGATGGGTG780.10565669700910274No Hit
GAGCACCGGGATTCTCAGGAAATTCAACCAGAAATGACACTTTGCCCTCCCC770.10430212397052449No Hit
AAGCCTAGGAGAGCTACCTTTTGACTAAAAATGTCATTTTTAATGAGCAGTA770.10430212397052449No Hit
GGGCCATGGAGGAGACTCCGTCTCAAACAAACAAAAACCTTCATCTTAAAAT770.10430212397052449No Hit
CTGTCTCAGAAAAAATAAATAAATAAAATAAATTAAAAAAGTAAATAAAGTG770.10430212397052449No Hit
TAGCAAAGGGGTCCCTAAAAGGAAAAGAGACGGGCCCAAAGTGGCATAATTT770.10430212397052449No Hit
CTTTGAGGGCACGTGAGGACGCACACTGGAGAGAAACCCTGTCAGTGTAAAC770.10430212397052449No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCCTGTCTCTTATAC770.10430212397052449No Hit
GAACACGGGGGTGAAAGAGGACCTAATTCTTGAATTATCTGCTTGAACTGTG770.10430212397052449No Hit
GAGGCCTGGAAATGCAACCCTTAACTACTGTTTTTCCCTGTACTTCAGTCCA770.10430212397052449No Hit
CTAGCAGGGATTGACTAAATCAATACTGAAAATGTCTATTGTTAAAAATACC770.10430212397052449No Hit
TTTCGCGGGGAGAGTGCTGAGTGAGAGATAAAGCCCAGGGGCAGGACTGGCA770.10430212397052449No Hit
TCAACTGGGGTAAACGATTTGGAAATAGGATGTTTTCAACGTTCTTTTGTCT760.10294755093194624No Hit
ATATCAGGGAAGTGAAATAAGCCAGACACAGAAGGACAAACAACATGTTCTC760.10294755093194624No Hit
AGGCAGGGGGGGGCGGGGGCCGGCAGGTGCTCCGCAGCCGTCTGTGCCACCC760.10294755093194624No Hit
TGTGGCGGGACCTTTCTAAAATTTGCAGAGAAGACTGACATCACTCATATTT760.10294755093194624No Hit
CACAGAGGGTGCACCATGAATTTGTCAAATGGATATTTATAGGAATATATTC760.10294755093194624No Hit
TTGCGAGGGGGAAGGAAATATGATCCTCCTGAGACTAACTTTGTATGTTAAG750.10159297789336802No Hit
CGCAAAGGGAGGCTCACTGAAACTTCTGTCTCCCAGGCTCGAGCGGTTCTCC750.10159297789336802No Hit
AGTACGGGAACTAAGGATGTCACAGAGGCTGGTCTCCTGGCTCGCAACACCT750.10159297789336802No Hit
GCGCTGGGATTGTAAAAAGAGTAAGAGAGGTTTGGTTTGTGGTGGTTTGCTT750.10159297789336802No Hit
AGGTATGGGCTGTGACACAGAGGCCAGGGTAGAGGTAGTACAGGTTGTAGGT750.10159297789336802No Hit
AACCGGGAGAAACACAAAGATCAAGGCTCAGCAGCTGATAGACTCAGCAACA750.10159297789336802No Hit
TCTATGGGGATGTCTGCATTCTTTTGCAACTGGAAAATTTCATGTTTCTTCA750.10159297789336802No Hit
GAAAATGGGCCAGGAAGCAATTAGAAGCAAAAGAGATTTGTTGGGGAAATGT750.10159297789336802No Hit
TCGCTAGGGGTAACTATATATGTTTACAAATTATGCATACAATTTTTGGAAT740.10023840485478977No Hit
TCTAAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA740.10023840485478977No Hit
GGGGTTGGGGGTGGTAGGCATAGGGGCAAGCCATGTAAGCTGAGGATTGGGG740.10023840485478977No Hit
AAAGCAGGGTGAAGAATGAACATTTCGATCATTATAATCAAGAAGCATTTAT740.10023840485478977No Hit
TTTGCGGGGTGGTTTACATCTAAATTAAGGGCAGGAGCTGTCTTTCAGACAT740.10023840485478977No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)