FastQCFastQC Report
Thu 19 Jan 2023
SRR5846230.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846230.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200283
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGCCGTCT19600.9786152594079377No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGC16890.8433067209897994No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGT2210.11034386343324197No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAGCG351.0062831E-746.0442431
TAATGCG351.0062831E-746.0442431
TTTAGCG253.3876833E-546.044241
AAATACG253.3876833E-546.044241
TTTCGAG253.3876833E-546.044241
CATTGCG253.3876833E-546.044241
CGCATAG600.046.044241
TTCGTAG301.8414194E-646.044241
GCGCATG301.8414194E-646.044241
TACATCG253.3876833E-546.044241
GATGCCG253.3876833E-546.044241
ACGTATG301.8414194E-646.044241
GGCGCAG301.8414194E-646.044241
TGCAACG301.8414194E-646.044241
TAATCCG253.3876833E-546.044241
GTCTGCG206.2691345E-446.0442351
ACATTCG206.2691345E-446.0442351
TGTTGCG206.2691345E-446.0442351
CTTATCG206.2691345E-446.0442351
CGCTAAG206.2691345E-446.0442351
Produced by FastQC (version 0.11.5)