FastQCFastQC Report
Sat 31 Dec 2022
SRR5846112.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846112.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101633
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCTCGTATGC7780.7654993948815837No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCTCGTATGCCGTCT7520.7399171528932532No Hit
TTCCCTGGAGGAAATTCAGAAGAAATTAGAAGCTGCAGAAGAAAGACGCAAG4770.46933574724744914No Hit
GTCACAGGGCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCT4200.41325160134995526No Hit
GTGGAGGGGCAGTCCTGCTCGGAAGCAGATGATGTGAAATGTTCCTTTCCAG3900.3837336298249584No Hit
GCTTTTGGGGCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGAT3680.36208711737329413No Hit
TCTAATGGGAGAGCAGTGCTGGAATAGCATCTCCTTTCACTTTCCCAAAACT3400.33453701061663044No Hit
CGGACTGGGAGCAATAGGATTTGCTGGTGAATTGATGTAGGTGTGACAGAAT3390.3335530782324639No Hit
CGTGGGGATACTACTGTTCATAAGAAGAAATTACTTTCTAAACAGTAACTGA3300.3246976867749648No Hit
AGATGTGGGGCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGA3040.29911544478663427No Hit
TTAATAGGGCGGCAGGGCATGACCTGGGGAGGGCAGGTGGAAGCCGATGGCT3040.29911544478663427No Hit
CCTACTGGGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA3020.2971475800183011No Hit
TATGGGGGGTGGCTGCTCATTGGCAATGCTATCAACTATAGATTGCTTGCAA2990.29419578286580145No Hit
CATATAGGGATGTAGATGTGTGATGGACCTAGACATTTTCATGTTTCAACAT2970.29222791809746834No Hit
TTTTGGGGGAAGTGTTCAAAACTCTGCAGCATTAAACAACGTGTATGCAAAT2960.2912439857133018No Hit
CATTGGGGTGACACCGTGGCTTTAAGGCATGGCCCATGATGTGTCTCCGAGT2960.2912439857133018No Hit
TGGTATGGGGTTGGAAACCTAAACTTTAACAAATCTGCTCCTGAATTAAAAA2930.2882921885608021No Hit
CACAGGGGAGAACTCAAGATTGAAAGGTCCACTCAAAACCACACAGCTACTT2920.2873082561766356No Hit
CGCACTGGGAACATTCTACTACTCACTCTCACTGCCCAAGAACTATCAAACT2870.2823885942558027No Hit
AACAAAGGGAGGGAGCTTCTGGCCCAAGGCCGTCTGAGGGATCTGCTCCTTA2860.28140466187163615No Hit
GCCTGCGGGCTCTGACTGGGATGGATCAACTCAGTTCTGTTATACATATTTA2800.27550106756663684No Hit
TGCAACAGGGCAAGTTATGCCTTCTGGCTACTACAGAATCTCCGCAGACCTT2690.26467781134080465No Hit
ACCAAAGGGAACATAGAGGGCATGGCAACTCTCTTTGACAGTGGTACCCCAT2640.2597581494199719No Hit
TCTCCAGGGATGAATGGCTCCACGAGGGTTCATCTGTCTCTTACTTTTAACC2630.2587742170358053No Hit
ACTAATAGGACTGTCAGGTGCTTCTGTTGCCATTTCTGGATGCTCGTTCTTT2470.24303129888914032No Hit
GCTTTAGGGAAGTCATGAGTCTAATTCCCCTGGGACATAGCTAGCCTTCACA2460.24204736650497377No Hit
CACACGGGGAAGGAAGAAAACCAAATGGATATTGTCTTGGACTTCTCCCTAG2400.23614377219997443No Hit
GTGCGAGGGCAAGGATTTTCGTATAATATTGTTTCATTTAATTCTCATCACG2390.23515983981580785No Hit
AATGCTGGGATCACTGAATCCTCACTCTCATTTTCTGAAGTAGTGTACCAGT2380.2341759074316413No Hit
GCCCAGGGGGATCTCTTAGCACATTTCTTAGGATTAAGGGTCCAAAAATGCT2370.23319197504747474No Hit
TACACCGGGGAGATTGAGTATCCTTTATCCAAAATGCTTAGGACCAGAAGTG2360.2322080426633082No Hit
TCAGAGGGATTAAAACTATTTACTGTTAAAAAATCTGTGACTTCATGAAGTT2290.22532051597414227No Hit
TGTTACGGGGGGTTCACTCATTCTCAGATTAAATTGCCACTTAAAGAAATAA2270.22335265120580913No Hit
TTCCGCGGGGCATGGTCACAGTAGAGATCCCCGCAACTCGCTTGTCCTTGGG2260.22236871882164258No Hit
GTATCCGGGTTTGCCTCAGACAGGTATCTCTTCGTTATCAGAAGAGTTGCTT2230.2194169216691429No Hit
CTTACTGGGGACTTCCAACCAAAGCCTTTGAGTATGCACTAAATAGGTGAGA2230.2194169216691429No Hit
TCAAAGGGGAAGCCACTGCTTCCTGGATGACAGCCAGACCCTGTCTCAAGAA2220.21843298928497634No Hit
GAGACGGGGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGC2180.2144972597483101No Hit
TGAAGGGGCGCTCTTCCGATCTACTCATGGGTATTCATGTAAGTCATATTTT2160.212529394979977No Hit
TACCCAGGGGTTCTAGAAAGTGTTAGGATGTGTAGAAACTAGAAATCTTGCG2160.212529394979977No Hit
CTCACATGGGAGACCGAGAAGTCTCGCGATAGCCAGCCGCGGCTGCCCTTGC2150.21154546259581042No Hit
AGCAGTGGGCATAGGCTTTTGAGTGCCTGAATTTCAGTTCATTCTGTAATTT2130.2095775978274773No Hit
ATGTTAGGGATGTATTAACAGAAAGATCCCAGAATTGTTGAGGGGGGATCAA2080.20465793590664447No Hit
TAGATCGGGAACATCCATGTGACTAGTGTATATGGTATTTGCTCTCCATTAG2060.2026900711383114No Hit
GTGTCTGGGCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAG1990.19580254444914544No Hit
CTGTATGGGAGAACTAAAAGCAGAAATAAAAAATTATAGAAAATTAGAAAAT1980.19481861206497889No Hit
ATAACTGGGCGATCTAGGTTAGGGATTCACAAATTTTCTCATGCTCGTTTCT1970.19383467968081233No Hit
ACACATGGGGTTGAAAAGACTGAAAGTTTAAAACATGAAAAAAATCAATATT1930.1898989501441461No Hit
TTTTTCGGGATGGATTATTAAAGTTGTCTACTCAAGTATAAAATATATGCAA1890.18596322060747986No Hit
TCGTCAGGGAATGGTAGGTATTACTTACCAAATGACACAATACCTTTTTGAA1850.18202749107081362No Hit
GTAAGCGGGAAAGAAAAAAATCTACAGCATTTTTCAGACTCCACCCTAGAAA1840.18104355868664707No Hit
ATTCTTGGGATCGCTGGATGCTGGTGGTATGATGGCAAGATGGGAACAGGTA1830.18005962630248049No Hit
TTGTACGGGGCATTATATGTGTGTTGGTTGTGGATTTTGTTTCTAAAATTGG1810.17809176153414735No Hit
AGTGACAGGGCTAATTAATTTGCTTTATACATTTTCTTTTACTTTCCTTTTT1790.17612389676581425No Hit
AATAATGGGCTACACGTGAAACATGCCAGGGCATGAGGCATGCCATTTACGA1740.17120423484498146No Hit
AAACTTGGGCTCATTCAGGTCTTAGATTGTAGTTAGCATTCCTATCTGTATG1690.16628457292414864No Hit
AAACGCGGGATTCCTCCATTGAGAGAGAGGCTCTATGGCCGATTAACCCAGA1680.16530064053998209No Hit
CGACTGGGAGAGGCGGGCAGATCACCTGAGGTTAGGAGTTTGAGACCGGTCT1670.16431670815581553No Hit
TCTAGAGGGAGTTACAGCAGGTGATTCAGCTACTTGAGGTCGGTAACAGACC1660.16333277577164898No Hit
TTCAGCGGGGAGACTGCGGGACGGACGGTGGACGCTGGGACGCGTTTGTAGC1660.16333277577164898No Hit
TCGTCAGGGAAAGCAAACTGGGGAGGTGGGCATTAGTATCTCTGTTTTGCCA1650.1623488433874824No Hit
TTTATTGGGAAGGAGGCAGACTGATGGCGATTCCCTCTCACCCGGGACTCTC1650.1623488433874824No Hit
AATCTAGGGTGTCCAGATCTGTTTATTCTTTTACAGTATTAAATGATTTTCA1650.1623488433874824No Hit
ATCTGTGGGATTCTAAAAGATGGCAAAACATAGGAACAAAATACAGATCAGT1640.16136491100331585No Hit
CGATTAGGGGGAGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCAC1620.15939704623498274No Hit
AGCTGGGGGATTGCAAAGTTGTTTATCTTCTATTTCTCCCAGGGACTATAAG1610.15841311385081616No Hit
GTCAAAGGGACACGGTACGTTTATGTTCAGTTAGATGACCAAAGCTTCTTAT1570.15447738431414992No Hit
CTGTATGGGAGTACAGTATATTGTTATGTACATATCATTGTTAATACAGTCC1530.15054165477748369No Hit
ATGCCTGGTAGGGAAAGGATACTATATTTAACAATGCTGCTGGGATAATTGG1520.14955772239331713No Hit
TCTATGGGGATACACTCTTCTCACTTGACATTTTAGCTATATAATATGTACT1500.14758985762498403No Hit
GCAGCAGGGCTTCGCTGACGCCATAAAACTCTTCACCAAAGACCCCCTAAAA1490.14660592524081745No Hit
TGATTGGGGATTATTCAGAGTCTTCAGCAAGGACATTGTATTTTACTTGTGT1490.14660592524081745No Hit
TGCGAGGGGATAGAAGGAACATGTGTCAGCATAATAAAAGCCATATACTACA1470.14463806047248431No Hit
AAGCACGGGACTGCTTCCAGGCTCCAAATGCGCCTTTCAATAAAGTAGTTCC1470.14463806047248431No Hit
GCCCGAGGGAGTTGGGAACCTCATTAGAAATGACCTCAGCTGCCCCATATCT1440.14168626331998466No Hit
AATTAGGGCTGTCCTTCCGCTCTACCATCCTAGTGCTTGGCACCCAACCTCC1430.14070233093581808No Hit
TAAGGTGGGAGTCCAGTTTAGTACTTTTTCTTTTCGTGTATTCAAGGTTAAA1430.14070233093581808No Hit
GAAAAGGGGACAGTGACGAAAGTGTCTACGGTCTGTGAAGCTGCTGCCCCTT1430.14070233093581808No Hit
GTGAACAGGGCTAAGTAAACGATTAAATGGTAGTGGGGAGCCTTCCAGGAAG1420.13971839855165152No Hit
CATGTGGGAAGTCAGTGATGGAACCTGCTTTATGACCAAGATTCATCCTCAA1360.13381480424665218No Hit
GTTTTTGGGACCTGAAACCGGAAACTCCTGAGAATAAGGTTGATTCTACACA1330.1308630070941525No Hit
GTTCAAGGGCTTTCAGGTGAACCCAATTCAGTGTCTTTAGACCACATTTTCA1330.1308630070941525No Hit
CTCTGGGGGACTCTTGATCCTGCCCTTGCTGCACTTGGGCTTCCTGGAGCAA1310.12889514232581936No Hit
TTTTGGGGAGGAATTCGAAGACCTTGGCTAGGTGGTGAGCTTGGAAGAGCTG1310.12889514232581936No Hit
TCTACTGGGATACAAAAATTAATGCAAGGTGGATTAAAGATTTAATTGTAAG1290.12692727755748626No Hit
GAACAGGGGTGGCTTAAGGCCATTCAGAAGCCAAGAAGTAGGAGTGGTGCAT1290.12692727755748626No Hit
CTTGGAGGGACTGGAGAATGCCCGAGGCCACCCCAAACTCCATGAGTTCAAC1280.1259433451733197No Hit
ACGTTTGGGTATGTGTAACTTCCCCCCAACCCCAAGCTGTTTCGGAAGATAT1280.1259433451733197No Hit
CGGATGGGGAAGGCTATTGAATGACCAGGTGGGGTAGCAGAGAGGAACACTG1280.1259433451733197No Hit
AAGTGTGGGATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC1270.12495941278915312No Hit
TGAACTGGGGAAAATCATCATGCAACACATGAAAAAAACATTAATCAGAAGT1250.12299154802082No Hit
TATACAGGGGATGAGGGGCTTCTACATTAGGAAAAGCATCCCAGTGCAGAAC1240.12200761563665345No Hit
AATGCGGGGACACCATTGTTCCTTTCACACTTTCCTTTGTTGCATGCAGTTG1220.12003975086832033No Hit
TGCATAGGGGATATAGTATCTTTATATAATGAGTGAATAAAATCAGAGTAAC1220.12003975086832033No Hit
GACGTAGGGCGTATGTTTTGGAGTAGTGGAATTGCCAGCCAGGCCCTGTGGC1210.11905581848415377No Hit
TAGCGGGGGAACTAAGTGCACCTGTGATCTTGGCCAAAAAACCATTGCAACT1210.11905581848415377No Hit
TATGTAGGGTTATGTATTTGTAGTTTTTACTCTCCTTATATTTTTCCTATCG1200.11807188609998721No Hit
CGAGTAGGGCGACCTCGGAGCAGAACCCAACCTCCGAGCAGTACATGCTAAG1200.11807188609998721No Hit
TGCCTTGGGTGCTAGAGCTATTTTCCTGAAATACATTTGCAAAATAAGGCTG1190.11708795371582065No Hit
CTGGCAGGGAATGTACCAGACCCTGGGGGGGGTCACTGGGCCCTAGATTTTT1190.11708795371582065No Hit
ATACATGGGAGAAGCTCTCCGGGCCCTCAGGGGCTTAGATCCCACTTGAGTC1180.1161040213316541No Hit
AAACTAGGGATGAGCAGTTCTCTAAAAACATAAAGCTTGGAATACACGAAGA1180.1161040213316541No Hit
GCGCTTGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA1150.11315222417915441No Hit
AGTTGGGCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCACAACT1150.11315222417915441No Hit
GCTTTTGGGGACTTCCCAAGAAAGACAGGTACAAACAAGCATAAAATTGTGA1140.11216829179498786No Hit
TTAACAGGGCATTGTTTCCCTTTAAAACAAAAAACAAATCAGTACCCCTGCC1130.11118435941082129No Hit
TACTTAGGGACTGCATCCTGTAGACATGGAGGGTTTCCCCCCTTATTCTGGG1130.11118435941082129No Hit
TATAGAGGGCTCTCTTATTTACACACTACCCATGTACACACCCTCTTCAGCC1120.11020042702665474No Hit
GTGTTAGGGAGATTTGAGACAACTAGCCTGTTATTCAGCCCAATATCCCCCC1090.10724862987415505No Hit
ACCACCGGGAAATTGGCAAGATGTATTTTTACTTTCCAAGATGCCGTGTGTG1090.10724862987415505No Hit
AATGCGGGAAGTCAAGTATCTTTTTACAGGACTTAAATTGCGACTTCAGGAA1040.10232896795332223No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCCTGTCTCTTATAC1030.1013450355691557No Hit
CTGTCCGGGCTTCCGATCTATGTAAGGGATATCAATCAATCAATCAATCAAT1030.1013450355691557No Hit
TTGCTTGGGATCCTGTTTTGACTAAGTCTTAGGTGGGTTGGGAAGACAGATA1020.10036110318498911No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)