FastQCFastQC Report
Sat 31 Dec 2022
SRR5845991.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845991.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86142
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGGAAGCATCTCGTATGCCGTCT7230.8393118339485965No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGGGAAGCATCTCGTATGC6770.7859116342782847No Hit
CTAGGTGGGAAGTGAATGAATGAGGCAGCATTGCTAATTTTTGTATAGTGAG1400.16252234682268812No Hit
TACGCTGGGTGCAGAAGGAAGCTATTGCTGCTCACAAGGAAAACAAGCTACA1380.16020059901093545No Hit
AAGACAGGGATCAAAAAAGATCTTTAAAGTATTCAGAAGCAACAGAAAGAAT1310.15207448166980103No Hit
CTTTTGGGGAGGAGGAGGCCATGACTCAACAGTTCTCCCAACCTGTTGGTGA1280.148591859952172No Hit
TTATGAGGGTACTGAATAACTGAAATGTCAGCAATTTAAAATAAATTCAATT1250.14510923823454297No Hit
TTTGTGGGGATACTCTCAGTTATATCAACAGGAGTCTTGTGTTTTGTTACCT1250.14510923823454297No Hit
CTTAGGGATCAAAAGAGTGTGACTTCTCATTTGTGAGTAGTTCACAAATTTC1240.14394836432866662No Hit
CGCATTGGGGTACTGGCCTGCACCAGAAGATGTCTGCATTACTCATTGCTAA1210.1404657426110376No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGGAAGC1190.1381439947992849No Hit
TACAAATGATTACTACAGGAATAGTGGCCACTTAATGTCAGTTACTCCGGTG1190.1381439947992849No Hit
GCATATGGGTCACTGCTAACACTCTCACTTCTTCATCACGTCTCCTTCTGAG1180.13698312089340856No Hit
GTGCTGGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTGA1160.13466137308165588No Hit
CGTACGGGGGATGGTAAATTCTGCTTGGTTGTTTATAAGCTCAAACCTTGGA1150.13350049917577952No Hit
GTATGAGGGATTCCACCCATCTTTACAGCAGAAGAATACATTACTTCCATTA1150.13350049917577952No Hit
CGTATGGGGGACGGATGGTCTGTGACTGTGAGAATCCCACAGTTGATCTTTT1140.13233962526990317No Hit
GCACAAGGGAGAGGAAGAATGGAGGAAGAGAAAAATACTTTAGACCTCAAAT1140.13233962526990317No Hit
GTATGAGGGTCGCCAGAGGTGCTTCACCCTGAGTTTTGTTTTGTATTGTTTT1140.13233962526990317No Hit
GCTAATGGGACAAGTGTCCAGTCTGGATGTCAAATTTTCATTAAGGGCATAG1140.13233962526990317No Hit
AGAAAAGGGGCTTTCCAACTTGGACGCTGCAGAATGGCTCCCGCAAAGAAGG1130.13117875136402685No Hit
AGCCTTGGGCGAGTTGCGGGAACAGATGTCAGGTTATAAGCGGATGCGGCGC1120.1300178774581505No Hit
CATACCGGGTTGAATGAATTAGTTCTGTTCTGTGTCAAATATGGCCATAACA1110.12885700355227417No Hit
GAATGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1110.12885700355227417No Hit
TGTAATGGGGCACACGCTGGTCACGCGGTCAGCTATTGACACTTCCTGGTGG1110.12885700355227417No Hit
GCATCGGGCAGGCACCATCATCTTTTGGCTTATGCTCAATGTTTGCCTACTG1100.1276961296463978No Hit
AACTCCGGGATTAAAAACATTTTCAGCTTCATCAGATGCTGACTAACTGACC1100.1276961296463978No Hit
ATCACAGGGGTTGTTTGAGTTTTGTATAATGATCTTATTTATTTTTAAAAAA1080.1253743818346451No Hit
AGTCAGGGGTCAAAAGGAAAAGAACTACCAATGAAATAAAAAATCTTCAGTA1080.1253743818346451No Hit
CAACTGGGTACTTCACCTCTGAGCCTCCGATGCTCTGTGAAATGGAAGGAAT1080.1253743818346451No Hit
CAAAATGGGGAACATTCATATTCCAGAAGACACAAATCTTTTCTTTCTATAT1080.1253743818346451No Hit
AGCGCTGGGTACGTGAAAAATGATACTCTGCAATTTTAACTTATATTTCTTT1070.12421350792876878No Hit
ATGGCTGGGAGCCTTTTATAACTCTAGGAGTTGAAGTGGAATTTTATCATTG1070.12421350792876878No Hit
AGTAACGGGGTATCAAGACCTATGCCACATCACACTGGGGCTAGGGAAGTAG1070.12421350792876878No Hit
AAACTGGGGCAATTTGAGATCTTTCTAGCTTTCTGATGCGGGCATTGAGTGC1070.12421350792876878No Hit
GCGTATGGGAATTAAGACAGAGTTCTGTTAATCTAATCAAGTTTGCTGTTAG1060.12305263402289243No Hit
CGTGGAGGGAGGTCTGGAAGGTTTGAAACATTTTCAAATAATTACTTGGTCA1060.12305263402289243No Hit
CATATAGGGATGGAGGGGCTTTAGCCATCAGCTTTGTACATCATCATTTTTC1050.12189176011701609No Hit
TAATCCGGGACTAGGGGGATTTGTGAAATGGAAAGAGAAAATTGTCATATTA1050.12189176011701609No Hit
TCCGTGGGGGAGATACAGTTGCCCCTATATGATCCTGTCTTAGTCCATTCAG1050.12189176011701609No Hit
TTTAGGGGGTTCTTTGTAATGTTCTTTGTTGCCATATGGTATTGTGGTCATA1050.12189176011701609No Hit
CGACAGGGCACTAATACAAGTTTAAATTTTTCAGAAAAATCAATCCCCAGTG1040.12073088621113975No Hit
GACTCCGGGTATTTAATTTTTGCATATTGTGGATTTCCCAGTTTTCCTTCTG1040.12073088621113975No Hit
TGGCTAGGGATAGCCCTCGTAGTAACAGCCATTCTCATCAAAACCCCCTGAA1040.12073088621113975No Hit
GTCATTGGGAAGCCGTGATCAGGTAACACGCAGTTTCTTAGTAGCTGCCTCT1030.11957001230526342No Hit
TGTACTGGGTCCCTAGGGGTACACAAGTCAATAAATGTGATACATCGCATAA1030.11957001230526342No Hit
CGTTGCGGGGGTGTTACTGAAGGGATATGGGAAATGTCGTGGACGGCCAGGG1020.11840913839938705No Hit
ACTAGTGGGGCCAAGCTAGTCCGGGATGCCTTTGCCCTGGCCAAGGAGAAAG1020.11840913839938705No Hit
CGTTGCGGGTGTTGAACCAGAGCTAGACATCTCAGACCAAAAAAGAATAGAT1020.11840913839938705No Hit
TTGCGCGGGCAAAGAAGCTTTTTCACTATTTGACAAAGATGGTGATGGAACT1010.11724826449351071No Hit
TTGCGAGGGACACACGACATGCGGCTTTTCTGCATCAACTGCTATGACGGTT1000.11608739058763437No Hit
GAACAAGGGTCGATTGATAGTTTTTCATAATTAGGAATAGTGTACTCTGTAT1000.11608739058763437No Hit
CGTATAGGGGGTCCTAGGATTCCTGAGCCTGTGAAGAATCAAGCTCCTATGG1000.11608739058763437No Hit
CGAACCGGGGCCACCACCCCTGCCTCTAGAACAATGCAACCAACCTTAACCA1000.11608739058763437No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGGGAAGCAACTCGTATGC1000.11608739058763437No Hit
AGGCTCGGGGCAATTCAATTTAACCCAAAGAGGGAGTCCTGGGAACCCCAAT1000.11608739058763437No Hit
CACATCGGGTAAAGGTTTGAGTTTCATGAGAAAAGAAGACTTTGCAGAGTGG990.11492651668175803No Hit
AGCACTGGGTGAGTGATGAAGTGACAAGGACGTAAGTTGAAATCAATACAAA990.11492651668175803No Hit
TAAAGAGGGTGTCTGTATTGCTAGAAGACACAAGTCTCTCTACTTTCTTGGA990.11492651668175803No Hit
CAGCCTGGGGGACAGAGCGAGACTGTCTCAAAAACAAACAAACAAACAAACA990.11492651668175803No Hit
TTTAGAGGGTCAATGCCCACGAGCAGCAAACCCAGCAGCTGAAACTGTCCCA990.11492651668175803No Hit
GCCTGGGTGACTGAGCAAGACCCCACCTCAAAAAATAATAATCCAGCCATGG990.11492651668175803No Hit
AGTTGCGGGTCAGCAGACAAAAACCAAAATGCCAGAACATGTTAATTAATGT990.11492651668175803No Hit
GCGAAAGGGGCTCCTGTCTTCTGGCTTCAGTCTGGAAGATCCCCAGACACAT990.11492651668175803No Hit
CTGACTGGGCAAGTTTTTCAATCTAACTAGAAGAGGTTGCGGTGCTGTAATG980.11376564277588168No Hit
GTACACAAGGGGCAGGACGATGCTCACATTTTTTGCACAGTGGAGCAGGTAA980.11376564277588168No Hit
GGATGTGGGATTTCTGAGTAAGGGGCTTTGTTGAAAGAGGGGGTTAGAGAGA970.11260476887000534No Hit
GTATCAGGGTCTGCTTATGACAATAATTGGCCCATTGCATGCAGAGAGAATG970.11260476887000534No Hit
AAAATAGGGCGAGGAATGGGAGGTAAAAAGTGGGGGCATTGCATTGGAATAG970.11260476887000534No Hit
GGATCTGGGTGCTTTATGCTCATAACTGATGTGGCTGGAGAATTGGTATTGA970.11260476887000534No Hit
AATTCAGGGCTTTGAAATGACAATTTATTAAGCAATGGTATAAAAAACCTGA970.11260476887000534No Hit
CGTACAGGGCTGAAACTCAAGCCTCAGAAACCTCTAGACCCAGCTCCCCTGA970.11260476887000534No Hit
CAAAATGGGGAACTCCTGACCTCAAGTGATCCACCTGCCTCGGCCTCCCAAA960.111443894964129No Hit
AATTCAGGGAAGAGAAAAAAACAGAAACAAAGTAAATGTTCACCAACAGGAG960.111443894964129No Hit
ATAACAGGGTCTATACAAGGGAGCATTTTTATGAACTTTGTGTTACAGTTTT960.111443894964129No Hit
GAGTGTGGGGTATAAGAAATATATCAGAAGTTTGTAGTAGATTAGAGAGTAC960.111443894964129No Hit
TGGCCAGGGGAGTGACCCGGAAGTAGAAGTGGCCCTTGCAGGCAAGAGTGCT960.111443894964129No Hit
GCGCCAAGGGAATGATCATTCTTATTGCAGATGGCCCTCTTTAAATGTTTCA960.111443894964129No Hit
GTTCTGGGGGCAGTAACAACAAAATTTGTTCGGACATCAACAAATAAAGTAA960.111443894964129No Hit
AGCACCGGGATTTGTAAGAATTGGAGCAATGTTGGCCTATACGCCCCTTGAT950.11028302105825266No Hit
TGACCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA950.11028302105825266No Hit
CAGGTGATCTGCCCGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAG950.11028302105825266No Hit
TATTTTGGGATCCTCATCTGGGACAAAAGCCTGGAGGTTCAGATTTCTTAAG950.11028302105825266No Hit
GATAACGGGGATGAAGCTTCCAGAGGAACGATCAGGCAGCAACATTTGCTGT950.11028302105825266No Hit
GCTTCTGGGGCTCTGTGTCCATGTACTTAATATTTTAAAGAGCTCTTACTTT950.11028302105825266No Hit
GCTACTGGGATTAGCTACAATTCTGCTGACCCTACTATTAAAGAATCTGGAT950.11028302105825266No Hit
AGCAGCACTCCACCTCCTATTCTTGCACGAAACGGGATCAAACAACCCCCTA940.10912214715237631No Hit
GACGCTGGGCTTCCGATCTAATGCTGGGCTACCTCTGTGTTTCACATGGCCT940.10912214715237631No Hit
ATAGTAGGGGTGCCCTCTTTGTCTACGAGAAGGGGGCTCGGGTGCTTCTGGT940.10912214715237631No Hit
GAGCATGGGCTGCCGGGCGCTCCGCGAAGATGCAGCTCAAGCCGATGGAGAT940.10912214715237631No Hit
AATGCAGGGGGTACTTGCATTTGACTTACGATGTATCTGTGAAAATGGGATG930.10796127324649997No Hit
ACGTATGGGCATGGTCAATTTTTAAACACGCATCAAGATCTGGAAGGCAAAA930.10796127324649997No Hit
TAAGGGGGAGGAGAGGTTGCTATGGTGACAGACTATGGGGCCTTTATCAAAA930.10796127324649997No Hit
CGTAGGGGGGTGGAAGGTGCTAAAAAGCTGGGCTTTGTACAGTGAGAACACC930.10796127324649997No Hit
GTGGGGGGAAGACAGACCTCTTGAAAGTTAATGTTTCAGTTACAAATCACTA930.10796127324649997No Hit
CTGCCGGGGAACAGTTGGCTACCTTTAATTGTCCAAAGCTTGGTGATTGGCA930.10796127324649997No Hit
CTAGTAGGGTAGAATGATTATAGTGATTTTATATTATTTGTAGAAAGACATG930.10796127324649997No Hit
AAAAACAGGGAGGAAACATGAAGCATAATTTGTTTAAAATTCTCTTTGGTCA920.10680039934062363No Hit
TCGAATGGGGTGGCCTTGGTCATTGTGGGAGACTTCTCTGTTGTTAATTACT920.10680039934062363No Hit
CGCACAGGGATTACCTTTCCAGTAAAAAGTATAGATAATTTAATTAACTTAG920.10680039934062363No Hit
CGTTAGGGTACACAGGGCACGATAGAATGTTACTGATGATTCAGGGCTATTT910.10563952543474726No Hit
TCAGCAGGGCTTTTTAAAATGGAGGCCAAAAATACATTTTCCAGATCAACAA910.10563952543474726No Hit
AATTTGGGATCCTTTTCAGAAAAAATATAATTGCAAATAGGTAGGAGTGGCC910.10563952543474726No Hit
GCGCACGGGGAAAGAAAAATATGAAAAGAGAAAAATATTTTAATTTTAAAAA910.10563952543474726No Hit
TTGTCAGGGACACAAATCCATTATTACTTATAAAGGTAAATTATACTATAGA910.10563952543474726No Hit
TGCATAGGGATCTGGGCCCAGATTGTCTGGTTGGCAAGAGCAAAGTTTCCGT910.10563952543474726No Hit
TTGTTCGGGCGTGTTAGCCAGGATGGTCTTGATCTCCTGACCTCATGATCTG900.10447865152887092No Hit
AAGCAGGGGATCCCATTCTAGTTACAGAGGAGCTTTCCTTAAATGCCCTTTA900.10447865152887092No Hit
AATGAGGGGACCTTTGTGGGATGCATGATAGAACTATTGACAGTTGGCAGAG900.10447865152887092No Hit
AACCTAGGGGAACAAGATGGTCTAGATTACAATAAGGACCCCATGGGACAGA900.10447865152887092No Hit
AGTTTGGGGAATTGTGCTTTATGATAAAACTGCCATGTACAATATAAACAAT900.10447865152887092No Hit
CGCTGAGGGCAAGCAATTAATCCCAAGTTGCTTCAGTTGGTCGAAGACAGAG900.10447865152887092No Hit
ATAGCCGGGTCTTCCGATTCTGTTAAAGGGTTAAGGAAAAGCGGGTCATTGC900.10447865152887092No Hit
AATGCAGGGACTAGAAGCCTCAGTTTCCCCATTTTACCCTTCCACACAATAG890.10331777762299459No Hit
ACACAAGGAATGATTTTTAACAACTATCTTCAAAGCTGCCTTTCACTGGCAT890.10331777762299459No Hit
TAATCTGGGGACTGTAAGTGGCTTCCGGGTCTGCGCGCACAGTGGCGGATGA890.10331777762299459No Hit
TGTCTGGGGACCTGAAGAGACTCTGAAGACCTACTGGGAGGATAACAAAAAC890.10331777762299459No Hit
AAAATGGGGAAAGTTAGATAATGGGTTTTATGGATTTCTTTGTTATAATATA890.10331777762299459No Hit
ATTCTGGGGAAGTATGGGCCAGGGAGAACTACAGCTACGAAGACCTGCTGTC890.10331777762299459No Hit
GATTCGGGCTGCACACATAACACACATCAGTCCACCCCCATTGTGAATGTTG890.10331777762299459No Hit
CTTTAAGGGTCTTGAGCAGAATTACCAATGCATTTGTCTTGAATGCTTAATA880.10215690371711825No Hit
AATCTTGGGAGACAAAATTGTAGGAAATCTTACATTTTTGCAATAACAAACA880.10215690371711825No Hit
TGCAGGGGGGAGCGTGAATGACATGGCCCTGGATGCCTTCGACTTGGACCGG880.10215690371711825No Hit
TAGACTAGGATCTCAGAAAACTGGTGGCTTCAGAGGCTTGCAAGATGAGAGT870.1009960298112419No Hit
TACATTGGGAAGTTACTGGCTGAGTGAGGGCATAGAAGTTAAAGGTTACTGT870.1009960298112419No Hit
TAAACGGGGGAGGAAAGTAAGACAATGTTATGAAGCAGAGATGGCTTCAAGG870.1009960298112419No Hit
GTTCGAGGGGAGCTTCTTACAGTGAAAGCAAAGTAGGTCGAGTCCACTGAAA870.1009960298112419No Hit
GCAAGTGGGATTTGAGAATGACATCAGATTCTAAATTGGTGAATCTGAAGGC870.1009960298112419No Hit
AACGCTGGGACCTTTTGGGGTCCCCAGTGTTGTTTCCTTCCTTATGTTCATG870.1009960298112419No Hit
TATGCAGGGAAACACATAAAGCACATACACACACCTCCAATAGATACCAGCT870.1009960298112419No Hit
CTCTGATGGGAGGTGGTACTTAATAATGTAGACTAGATAGCAGTAGAAACAG870.1009960298112419No Hit
GAGTAGGGGCATAAGCTAGAGTGAAAACCTTGTATTAATGGATTTTCTTTCA870.1009960298112419No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)