FastQCFastQC Report
Sat 31 Dec 2022
SRR5845969.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845969.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121379
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGTTACATCTCGTATGC4850.39957488527669527No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTTACATCTCGTATGCCGTCT4830.39792715379101823RNA PCR Primer, Index 14 (95% over 22bp)
CTGACAGGGGGCCGGTCGCCCAGACATGGCGGACGGCAGCGGCTGGCAGCCG1660.1367617133111988No Hit
GCTAAGGGGGAGTCATGATCACCGAGACCGCGGCGGAGCCTACGGTCCCTGC1490.12275599568294351No Hit
AATTATGGGATGCTGCCTGCAGCCATGAAGGGCCTCGGCCTGGCGCTGCTGG1470.1211082641972664No Hit
GAATTCGGGGGACTTTGCGGAGCCATGGAGGGCGGCTTCGGCTCCGATTTCG1450.11946053271158932No Hit
CTTGGCGGGAAGTATGTGGAATTCTAATTAACTCCTAAAGTGGTCATTTTCA1440.11863666696875078No Hit
AAGCTAGGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCT1430.11781280122591221No Hit
GAACACGGGGAAAATTGCATTGATACTAAATATTGTGGCACTTCGGGAAACC1430.11781280122591221No Hit
CATCCAGGGACTCGCGCAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGTGGC1400.11534120399739659No Hit
AACTCAGGGGTTGTAAGGGGCCAATTCTAAGTATTCTTCACGGCAGCCAGAA1380.11369347251171949No Hit
AAAGTGGGGACTGTACATCAAAGGATTCATACCGGAGAAAAACCATATGAAT1370.11286960676888094No Hit
AGTCAAGGGACACGGACCAGTGTGCAGAAGGCTCCGGGAACCGCAGATTATG1360.1120457410260424No Hit
CACCTAGGGATTTTAGGTATTGGCAATATTCCAATAAGCATGATAAAAATTG1350.11122187528320386No Hit
TGGTCGGGGGACTGCGTTGAAAGCTTCTGAATTTCAAGCAGAAAGTAGAAGG1350.11122187528320386No Hit
TGCATTGGGGTACATCAAGCTTTCACTGATTCCCTAAGATTTTTGTTTCCTC1330.10957414379752675No Hit
GATTTGGGTCTCTCATACCTCTGGGAAACAGACCTTTTTCTCTCTTACTGCT1320.1087502780546882No Hit
ACCAAAGGGAGTTTGATCCATTTCCATCCATCTGCTCATGAGAGAAGAAATT1310.10792641231184967No Hit
GTGCGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1310.10792641231184967No Hit
AAGCTGGGGATTCTGGGTCCTAGATTACTGTCATTGTCTAAAAGTAACTTTT1310.10792641231184967No Hit
TAGCTCGGGATCTTTAGCCAGACTCCTCAATCCATCTGCCTGTTGCACTTTT1310.10792641231184967No Hit
TCACGTGGGAGGGGGGCCGTCTGCAGCGCGTGGAGGTGACCTGGCGAGGCTC1290.10627868082617256No Hit
TGGGAGGGATTCAGCGGGTGCCAAAGCCTTTGTCTGTGATCAGTGCGGTGCA1280.10545481508333401No Hit
CATGCAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1280.10545481508333401No Hit
GAATATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1270.10463094934049548No Hit
AGTTATGGGACACAAAGCCTCAGCATCAGTCTCACACCTAACGTGATGCTGG1270.10463094934049548No Hit
TTTTGCGGGGATCTTGGCTCACTGCAACCTCCACCTCCCGGGTTCAAGTGAT1260.10380708359765693No Hit
CCATGAGGGAGATGAGGGGCAGGCAGTCAGGCAGAAATGGGCAAGTTCAGGG1260.10380708359765693No Hit
CTGCTGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1260.10380708359765693No Hit
GTGTGCGGGTTAGAATCCTGGTTTCTTCTTTTTCTGAGGAGCAGCTGAACCG1250.10298321785481838No Hit
TGTGCCTGGGCTGTGGACTGAGCCCTTTATTCCCTCTCACACCACCCTCCGT1240.10215935211197982No Hit
CGTGATGGGTATGGAATGCTAACAGAGCTCCTTCGGGGTGAAGATAAATGAC1240.10215935211197982No Hit
ATGCTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGTTTCTGCTGAGGGGGCA1240.10215935211197982No Hit
TTGCTCGGGGCATCGCGGAGACCAGGCCGGCGGGCGCGCCCGAAGCGCCCAT1240.10215935211197982No Hit
CCATTTGGGGTTATTAGTGACTGTCAAAGGAAGCTTTTTTAAAGGTCTGCCT1230.10133548636914128No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)