FastQCFastQC Report
Sat 31 Dec 2022
SRR5845954.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845954.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136769
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGTTACATCTCGTATGC4900.35826832103766204No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTTACATCTCGTATGCCGTCT4630.338527005388648RNA PCR Primer, Index 14 (95% over 22bp)
GAGTCATCTGACACTGATGATGAAGAATTACGGCATCGAATCCGGCAAAAAC1740.12722181196031265No Hit
AGACATGGGTTCAAGAGTTTATCTAGAATAGACACCTAGACATGTAGTTATT1710.12502833244375552No Hit
GCACTAGGGGTCTTTTCTTTTTCTAGTGGCAGTAATTTAGTGGATGTTTCAT1590.11625441437752707No Hit
GGAGCAGGGACTAATTATCAGAAAAATGCAAACCAAAATCACAGTGAGATAT1510.1104051356667081No Hit
TCCGCCGGGACTAGAAGAAGACAATAAAATGTTGGTGAATGAATTGAATCAT1480.10821165615015099No Hit
CTGGGCGGGATGTTCACTTACAAATATTCATAAATAAGTGTTCACTTACAAA1480.10821165615015099No Hit
CGCATAGGGACATGATTCTTGTTGTATCTGGAAGCTCTCAGGCCAAGGTGAT1430.10455585695588912No Hit
TGCTTAGGGATCTGACTTTCGATTGACTTGGTTCTGTGGAATAATGATCTGG1420.10382469711703675No Hit
GCTGCAGAGCGAGCCCAGCCTCACCAGCGACGAGCTGCACCTGCCCGGGAAG1400.102362377439332No Hit
ACTACCGGGAGGTAGCGGTAGCGATGGACACTCTGGATCGAGTAGTAAAGCC1400.102362377439332No Hit
GCGTAAGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG1400.102362377439332No Hit
AAACCAGGGGTTATTGCTCTTTGTGTTGCCTGCAGTATTTTTCTTCTCATTT1400.102362377439332No Hit
CGCAGCAGGGGGTTACATTTGGCCTCTTTTAAGTCTGTGAGACATGTTTAAA1380.10090005776162726No Hit
ACGTAAGGGCAGACTGCCTCAGCCCATGTGCCCTGCCGGCCAGGGCGTGGGC1370.10016889792277489No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)