FastQCFastQC Report
Sat 31 Dec 2022
SRR5845552.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845552.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85510
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCATGCACATCTCGTATGCCGTCT5350.6256578177990879No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCATGCACATCTCGTATGC4880.5706934861419717No Hit
GCTTATGGGACGCCCTTGTAATCCTAGCTACTTGGGAGTCTGAGGCGGGAGA1490.1742486258917086No Hit
TCCGCCGGGGATATCTGTCTTTCCGTGTCTGGCTTAGTTCACTAAAAAAATG1340.15670681791603322No Hit
GATTTGGGGTGACGGAGGTTGAGATGAAGCTTCTTCATGGAGTAAAAAATGT1310.15319845632089812No Hit
CGTACAGGGCAACAACATCCAAGCTGAGAGCCAAATCAAGAATGCAATCCCA1300.1520290024558531No Hit
CATTAAGGGAAAGAATAAGTATTGAAATGAAAGAATGACAGAAAGAATGAAT1270.14852064086071803No Hit
TTTATAGGGCAACACTCCACTTTCTGCATTGGGCAGATCTTGAAGACAGAAA1190.13916500994035785No Hit
GTCATGGGGATAAAAGGAAACCTTTTGTTGCTCCCAAGAAGAAAAGAAAAAA1170.1368261022102678No Hit
GACTTTGGGGTCAGCTCACTGCAAGCTCTTCCTCCCGGGTTCACGCCATTCT1160.13565664834522276No Hit
CCAAAAGGGGAAGCCTGAAAAGGAGGAGCAACCTGTCCAGAATCCCCGCAGG1140.1333177406151327No Hit
ATAACTGGGAGCAGCCAAGATGTTCAGTAAAGCCTTTGACAGTGGCATTATC1130.1321482867500877No Hit
CTAGGTGGGATAATAGGCTTGAAAATTGATATCCTGTGGTGCTAAAGTACAG1130.1321482867500877No Hit
ATATCCGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG1080.12630101742486258No Hit
GCAAAAGGGCACGCCACCCGCGTCATCGCCCTTATCAACGACCAGCGCCGGG1060.12396210969477255No Hit
AAGCAGGGGGGCAGTGCTAAGTAGATGACTGATGCTGAAATTTTACTAGATA1060.12396210969477255No Hit
CGCCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1050.12279265582972752No Hit
GCATGCAGTTTGGAAATAAAATTTAAACTCATTTATGTGTAATAAATATGTA1050.12279265582972752No Hit
GAGACAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1050.12279265582972752No Hit
GGTTCGGGGCAGAGCAGAAGCAGCTGCTGGAGGTGGAACTCTACGCAGACTA1050.12279265582972752No Hit
TCCTTGGGGGACGTACACCAATGCGGACAAGCTCCTAGAAGCAGCAGAGCAG1040.1216232019646825No Hit
GATGCTGGGGAATCAATTCAACAAGAAGAGCTAACTATCCTAAATATGTATG1030.12045374809963748No Hit
TGGAACGGGAACTCAACGACAATAATGACACAACCTATCAAACCTCTGGGAT1010.11811484036954743No Hit
GTTCATGGGCATAAAAGTCCTGTCACCAAGGACGTTAAAGGCATTTTATTCC1010.11811484036954743No Hit
GCGCTAGGGAGCTCAAACTGTCCATATACTGTTGACCAACTTTGAGGCTGTT1010.11811484036954743No Hit
TAGTCTGGGCACGAACACGTTTTTATTTTTCTCTTTTTATGAAGGCTTGCTC990.11577593263945736No Hit
ACTGGCGGGCAGACAGTGCTTGAGCTGCAGAAACTGAGACCAGACCTCTGGC990.11577593263945736No Hit
CTAGCTGGGAGAAAAGAGATATGTTTTCTGGAGTCATAAAGGAATTCAATTC990.11577593263945736No Hit
GAAATTGGGGGTGGCCAGCCAGTACGGATGGTCTGGCAACATGGAGAGAATC990.11577593263945736No Hit
GCTGCAGGGATGAATTCTTTAGTTCTGACATATTATTTTCTATTTTTAGCAT980.11460647877441234No Hit
CTCTCGGGGGACAAACGCATGTATGGCAACAAAAAGAAGATTTGAAAGGGAA980.11460647877441234No Hit
AAGCGGGTAGAAGTCCTAGCAGAGCAAATAGACAAGAAAAAGAAATTAAAAA970.11343702490936731No Hit
TAGTATGGGATGGGAAAGTGGTGGTGTTTCAGACTCAGCCCATAGTGGAGAG970.11343702490936731No Hit
CGCTATGGGGATGCCAAAATCTATCAGATTTATGAAGGTACTTCACAAATTC950.11109811717927727No Hit
ACACTAGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATT950.11109811717927727No Hit
AGCAATGGGGATGGCCGACGTGGAAGACGGAGAGGAAACCTGCGCCCTGGCC950.11109811717927727No Hit
TGTTAAGGGCATATGGCCTACAATGTCTCTCCCAGTCAAGGTTATATATAAT950.11109811717927727No Hit
TCTTAAGGGATCGTAAAACATGAGCCTTTCCAGAGTCAGCTTAGACACTGTT950.11109811717927727No Hit
CTTCGGTGGGGATGAAGGATTCAAGGTCAAGTTTACTCAAATAGGTTCTCCT940.10992866331423225No Hit
TAACACGGGATGCCTTACTGATACATTTGCATTTTGTCTACTAGTTACGGTT940.10992866331423225No Hit
ATTTTAGGGCATAGGAGGAGGAAGAAAAGCTAAAAAACAAACAAACAAATAA940.10992866331423225No Hit
AAGACAGGGCAATTGTTGCCATTGAAAACCCTGCTGATGTCAGTGTTATATC930.10875920944918722No Hit
TGTCGTAGGGGTAACTGAACCTACCAGGAGAAATGTTTGGAATTCTATATGT930.10875920944918722No Hit
CAGAAAGGGGGGGAGAAACCAGAGCAGAAAAGCTGAAAATTCTAAAAACCAG920.1075897555841422No Hit
CATAGCGGGTATATGTTTTGTCTCGCTATACTGCACTTACGCTATCCAGTTG920.1075897555841422No Hit
ATGCGAGGGTGCCTCTAAACCACTTTTATTTGTGTGGTTTTAGACGAATTGA920.1075897555841422No Hit
GTATTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC920.1075897555841422No Hit
TAAACCGGGCAGCCGGGGATTGTTTCTCCTATTTTATGAGAAGGATTGTGTA910.10642030171909718No Hit
TTAGTGGGGCCTAGGTTTCGAATTTCTGTCTTGTTACGATTTTTCTAACTTG910.10642030171909718No Hit
GGTGCAGGGTGGTGGTGGCTTTAAACAACATAAAGTTTTATCTTACAGTTCT910.10642030171909718No Hit
ATTGGTGGGACTTCGAGAACTTCCTGGAGACCATTGGCGTGAAGGATGGCCG910.10642030171909718No Hit
AAGCAGGGCTACAAAATTGGTGGTATTGGTACTGTTCCTGTTGGCCGAGTGG910.10642030171909718No Hit
GCTTTGGGGCAACTTGGTCAATCTACATGGTGTGACAACAGATATAAGGAAA900.10525084785405216No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG900.10525084785405216No Hit
CCATGGAGGGAAGTTTGAAAACCGATGAACCTTATTCTGATTATGTAGATAA900.10525084785405216No Hit
GTCTAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA890.10408139398900713No Hit
CAAAGCGGGGAGCACAGGGAACAGGCCGCCACAGAAAAGGTTGCCTTTTATG890.10408139398900713No Hit
CTCAAAGGGGAATGTCCGGTCAAAAAATAAGCGTTCCTTCCGAGAAAAACAG890.10408139398900713No Hit
TCCAAGGGGACTTCTTTGGGGATTTTGGTTTCATGTTTGGAGGAACCCCTCG890.10408139398900713No Hit
ATTGGCGGGAAGACCTGAAACCATAAAAATTCTAGAAGACTACCTAGGAAAA870.10174248625891709No Hit
GATACTGGGGTTACCTTAGGGGTCCTACTTAATTTTACTTATGGAATACTAA870.10174248625891709No Hit
TATCGAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC860.10057303239387207No Hit
ACTGGGGGACATGAAGGAAATAACTGATGTAATTGACTAAATCAATACTGAA860.10057303239387207No Hit
ATTCTTGGGATGACACTGACACTGCTATTTTGAAACCTGGAGGATGGAAAGG860.10057303239387207No Hit
CCACCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA860.10057303239387207No Hit
TAGAACGGGCATGCAAAAAAACATTTAATCTGCATCTGTTTTAGAAAAGGGG860.10057303239387207No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)