FastQCFastQC Report
Sat 31 Dec 2022
SRR5845508.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845508.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132358
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCAGTGTGATCTCGTATGC4060.306743831124677No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGTGTGATCTCGTATGCCGTCT4020.30372172441408907No Hit
GTGTAAGGGATTGCTCATGTAATGGGACTTCAGGGACAGCAGAGCAGGCAAC2690.2032366762870397No Hit
GTGAAAGGGGAATCACCACCAAAAGAATAAATCATCTAGGAATACAGCTAAC2140.16168270901645537No Hit
AACTACGGGATTACAGCTCATGTACGTCTGTTTTTATAAGATCAATATTAAA1830.13826138200939875No Hit
TCACTGGGGTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGAT1780.13448374862116383No Hit
CAGCCTGGGTGACAGAGCGTGACTCCGTCTCAAAAACAAAGCACAAAAGAAC1720.12995058855528188No Hit
ACTGGTGGGGGTTATAGAAAAAGTCAAAAGGAAAATAAAGATATATTTTGAG1710.1291950618776349No Hit
AATTGCGGGAAGAACAAAGACACAACATAGCAGTATCTCTGGGACACATTTA1690.12768400852234094No Hit
GTTTGGGGAGTGAGCAGGAGTAGCTATTCCTATATTAGACAAAACAAACTTT1670.12617295516704696No Hit
TCATAGGGGAGCAGGAATCAATGAAATTGATAACAGAAAAAAGTAGAGAAAA1650.12466190181175296No Hit
CTTCGAGGGGGGTGGCACCTCCAGAGTGAAGAAGATCCTTTGTCAAGAAGGG1650.12466190181175296No Hit
TTCATAGGGCTTTTTATTCTTTTTTCTCTAAACTTCCCTTCTCACTTCATTT1640.12390637513410598No Hit
TTGCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1630.12315084845645899No Hit
GGTTAACGGGGTGGCACATTATGAATCTACTCAGTCTTTGCTTGGAATTTAT1620.122395321778812No Hit
ATTCTTGGGGGATGTAGAATTAGGGTGTGGTATGGTGAGGGAAAAAGGCATT1610.12163979510116502No Hit
CCTATTGGGCAGTTTTGAGAAGTCATATAGAAGGCATATATTCCCTTGGTAA1600.12088426842351803No Hit
AACTAAGGGGAAGCTGTTCAGAAAAAAGTTAACATTGCAGTCCTGAGACTGC1580.11937321506822407No Hit
TTGTTGGGGCAGGAATACAAGTCCATTCACTATTCAAATGGCGTCTTGCTTT1580.11937321506822407No Hit
CTTAAAGGGCTACCTGCTGTTTTCTGATGACCTCTGGGATCTTTTCATTTAG1570.11861768839057708No Hit
TAAAGCGGGGCAATAAGCAAAGCAAAATAAAATTTGAAGTTGGGGTGATTTG1560.1178621617129301No Hit
GGCAACGGGGCATCTCAAATGTTTTCTGCAGAAAAAAATAAAAAGATTCTAA1550.11710663503528308No Hit
ACTCAGGGATTGCTTGAAGATTTTTAGTTTTAGGCAATCATATCTGTCGATC1530.11559558167998911No Hit
GACCAAGGGCTAGTCCTAGAAAATAAACCAAGGCCATATCAAATAAGAAATC1530.11559558167998911No Hit
GGCTGGGACAAGCTGGAATGTTCTCCCATGGTTGTTTTTGGGGAAAACTGGA1530.11559558167998911No Hit
TGCACTGGGGGATCCTTCTGGACTCTTGCCTCCCCTTGAAATTTCCCCCATG1530.11559558167998911No Hit
CTAGATGGGGATTTGTAATTTTCCTTTTTTATATTGACTTTGGTTTGATATT1510.11408452832469514No Hit
GGTAATGGGCTCCGCACCACCCCACTTCCATTTTGGCCAAACCAACCGCACC1510.11408452832469514No Hit
GTGGGCGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGG1510.11408452832469514No Hit
AGTATAGGGGTACAAGACCCTGTCTCGAAAAAAGGAAACCTTATTTTTTGGT1500.11332900164704815No Hit
AAACCAGGGATAATAGAAATGTATTTGTGTTTCTAATAACCAATGAGTATAT1490.11257347496940118No Hit
CTTACAGGGCAGATATGAAGACTATGATTACCGATACAGAAGACGATCACCT1480.11181794829175419No Hit
GGTCGGGCACAGTCAATAACGAAATACAGCTGGGCTTAGAAGAGTTGTGGGA1480.11181794829175419No Hit
GATCGCGGGGTTAATTGGGCAGATTTCAGCACTTGTCTTGCTCATGCTATTC1480.11181794829175419No Hit
TTGTTCGGGTAAGTTTTCTCTTTTAAGGTAGAATCTTGTTAATTTCATTCCA1480.11181794829175419No Hit
GTTTCTGGGTAACATACTTCTTGCAGCCTTTAATACTCACTTTCACAAATGG1470.1110624216141072No Hit
ATAGTAGGGATCTGTAAGAAGGTACTGGAACTTTCAGGGCTTGAATAACAAT1470.1110624216141072No Hit
TTCACCGGGACCACACGAGGCTGAGGCACCCCTGTGTATTCAGGATGGGAAA1470.1110624216141072No Hit
ATCAGTGGGTATGTGTTTTACCCAACATCATAATATATCCTATGAAAAAATT1470.1110624216141072No Hit
ACCGTTGGGAGGTAAGATAAGATTTACAAATAATGGAATATCTTTATTAACC1460.11030689493646022No Hit
ATGCATGGGCCAACACGACAGGTGCTATGAAACCTGTGGCAAAAGCAAGAAT1460.11030689493646022No Hit
TTTTATGGGCATTAGTGGTACAGTCAATATTATTTTCAAGATTAAGAGAAGC1460.11030689493646022No Hit
GCTCACTTCTGGTCTTTTTGGCCGGGAACTCCTGATTGGTGTTAAGGTGGTA1460.11030689493646022No Hit
ACATAAGGGGGCCGCTTGGAGGTATTTCCTGCCCACATCATATAAGAAGTTA1450.10955136825881323No Hit
GATTATGGGCTAATCTGTAGCTTTCTGGGGTTATCTGAATTGTTTCCAGGGC1450.10955136825881323No Hit
GATTAGGGGGTTGCTGTTTTAGGCCACCCCAGCTCAGCCAATGATTCCTTTC1450.10955136825881323No Hit
TGATAGGGGACCAAAACACCAGTGAAATTCTGGTTGCTAAAAAATTATCTCT1440.10879584158116622No Hit
TGAAAAGGGACGGAAAGAGAAGGGTTCACGGGAAGAGAAGCAGAAGCCCCAG1440.10879584158116622No Hit
TGTGTGGGGGAATCTCACCACATATACTTTGTTAGATTTGTGTATTGTAGGG1440.10879584158116622No Hit
GTCAATGGGTATAACATTGTGTAGACAATTAGTGGTACCAAGCCAGTCTGAC1430.10804031490351923No Hit
GTTCCGGGGAAACTGGATGACATTATGCTGGATGTTATAGGCCAGAGAAGGA1430.10804031490351923No Hit
TTGCTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1430.10804031490351923No Hit
TATAGTGGGATGGGTCTGGGATGTAAAATTGAATGAAATAGAATCTCTGAAT1420.10728478822587224No Hit
TTAAATGGGCATGGTTGCTGTTCTATTAGAGCCATTTAATCTCATTTGTCTC1420.10728478822587224No Hit
GTGGCTGGGTTGTGAAAACCTGAATGCTGAAACAAATATATTTTGTTGCAGA1410.10652926154822526No Hit
AGACATGGGGATGCCTGAGGTAGAAATAGTAGAGTTTTGGCTGCAAAATGCT1410.10652926154822526No Hit
AACTATGGGATGTTACAAGGACTGACTATATGTAGAAGGAAAATGTGACAAT1410.10652926154822526No Hit
AACTAAGGGCACAAGAGTCCCCGCATGAGGGAAGCAGGAAGGTCAGAACCTG1410.10652926154822526No Hit
TAGAACGGGGAAATTTAACATAATGAAACATTTCCTTAAAAAAGAAAAAAGC1410.10652926154822526No Hit
AGGTGTGGGGTTGACTGTGATGTTGGGTAAGTTACTTATCTTTGTACATCAG1410.10652926154822526No Hit
AGCATCGGGCAATTTAGGAATGCTCAATGTGCTGTTGATTCTTTCAATCCAC1400.10577373487057828No Hit
GATGCAGGGAAGTTTTAGTTAAACATAGAAAATATCTGCCCATTATCATCAA1400.10577373487057828No Hit
GAACCAGGGTATCTTGGGTGTCGAATGTGTCCATTCCATGCAGAACCACAGC1400.10577373487057828No Hit
TTTCATGGGAAACCACTGCTCAATGAAATAAAAGAGGACACAAACAAATGGA1390.1050182081929313No Hit
ACCTCAGGGATTATCAGCAAGATAAAGACAGATCTTATTATCTGTCCTTAAA1390.1050182081929313No Hit
TAGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAA1390.1050182081929313No Hit
TTTAGGAGGGCCAGGAAAGTACATTTGCATTAAAAAGTGCAACGGACAGAAA1380.10426268151528431No Hit
AGCCATGGGCCGCTGCAGTACTCTGGGGGAGAATGCTACAAAATTTGTTCGT1380.10426268151528431No Hit
ACTGATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1380.10426268151528431No Hit
GCTGGAGTTTACAGCTCAACATTCTTCATAGCCACAGGTTTTCACAGGCTTC1370.10350715483763732No Hit
CTCCCAGGGATCCTCGGTTTTCCTACTGACACTTTCGATTGCTTTCATTTGT1370.10350715483763732No Hit
TAAGCAGGGATACCAATATCTGTGGAACACACTGAAGGCAATACTAAAAGGA1370.10350715483763732No Hit
ATGCCCGGGAGGAAACCTAGATTCTGAGTATAGGTAATCCCAAATTAGCTTC1370.10350715483763732No Hit
TAAAAGGGGTCGCAGCCTGCTTTTCTTAGATGTTTTCTTGCTGTTCCTTGTC1360.10275162815999034No Hit
AGCAGTGAGCCGAGATTTCTTAGAAGGAATTGTTACTTATTGTTCTGAAGTG1360.10275162815999034No Hit
CCAGTGGGGGTCACATGGCCACCCCATCTTCCAGGAGGGTAAGAAAGTGTTA1360.10275162815999034No Hit
GCTCATGGGCACCTATGTTAAAACCTTATTTCACATTCCACATCATTTTAGA1360.10275162815999034No Hit
GCCTCTGGGGTAGCCACTGCTGTTTGCCCTGGCCCATCAATGAGAAGATCTG1360.10275162815999034No Hit
CACGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1350.10199610148234335No Hit
TATAAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1340.10124057480469636No Hit
GTGTCAGGGAGAGGCCTCATTTTAACTGAATCACTCCTTTAAAGATCTTATC1340.10124057480469636No Hit
CAATTTGGGATACCCAGGAATTCTTCCAAAGCGAAAAAAGAAAATCAAACTA1340.10124057480469636No Hit
ATAATGGGGAGAAGTGGCTGGATTGGAACTTTTAATATGCTAATGTGGAAAA1340.10124057480469636No Hit
GATTGGGCGGACAACTGGTTGCTTACACTGTTTTCAGAGATGAAATAGGAAC1330.10048504812704935No Hit
TTCAGTGGGGTATCCTTTGAACGTGAAAAGCTTCTTTTGCCAGTAAGATGAA1330.10048504812704935No Hit
AGCCCTGGGATAGAAGCTGAGTCTCTGATCCAATATGTTTTTATTTGTTCCA1330.10048504812704935No Hit
GTCCTAGGGGGAAAATACCTACCTTGTTGAACTTTGTATTCAGGGTTATGTC1330.10048504812704935No Hit
GCCAACGGGCAGCAGTTAGCATGTTATGTTTTTCCATTGTCTAACAACTTTA1330.10048504812704935No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)