FastQCFastQC Report
Sat 31 Dec 2022
SRR5845478.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845478.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211564
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCAGTGTGATCTCGTATGC7410.3502486245296932No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGTGTGATCTCGTATGCCGTCT6420.3034542738840256No Hit
ACTATTGGGACACACTGACATTGTAATTTAAGTTGATGTATTTTTGATTCTG3300.15598116881889168No Hit
ACTTATGGGATCCAAAATTAATAAGTATCTGTTGGCCTCTGTTTTCTCCCAG3210.1517271369420128No Hit
CATATCGGGGAATCAGTATTCCATAGTATTTGCTTTCCTTGTATCTTCTTGA3070.1451097540224235No Hit
AAGCCTGGGAAAGAACTGAAATCATAACCAACAGTCTCTTGGACCAGAGCAC3040.1436917433967972No Hit
TTTCTTGGGTGGAGATCCCAAATTTATAAAACAATTACTACTAGACATAAGA2990.1413283923540867No Hit
ATTGTGGGATGACAAAGACCTCAAAAGCAAATGCAACAGAATAAAAAAATAG2960.13991038172846043No Hit
AACATCTGGGGATCATAACTAGTAATATGAGTTAATGTTGAATCTTTTTTGA2930.13849237110283413No Hit
TTGAGCGGGCATGTGGATCTAAATTTTAAAAATAGGAATGAATCTCTTAATT2900.13707436047720784No Hit
GAATTTGGGCACACAAACTCCAAGGTGTTTTTAAGAAGACCAGTGAGGGATC2880.13612902006012367No Hit
GGTACAGGGCTCTACAGGAACTTAGTTCTTGTATGGCAACTTGAGTGGCATT2840.13423833922595527No Hit
GTTTGCGGGTAAAACTTTTTTGTATTATTGTTTGTTTTGTGTTCAGTTCAAA2830.13376566901741316No Hit
GAACCCGGGTGGCAGCTGTGGCGCCGACATGGCTGCGCTGGTGGAGCCGCTG2820.13329299880887108No Hit
TGTGGGCGGGATTCACATAACATAAAAGTAACCATTTTAAAGTGTACAATTC2820.13329299880887108No Hit
TAAACTGGGATAGTTGGCATCCAGTAATCAACAATAAATAGGGAAAAAATCA2790.13187498818324478No Hit
CGGTATGGGCTTACTCGGGAGCTGGCTGAAGATGGCTACTCTGGAGTTGAGG2790.13187498818324478No Hit
GGCTCGGGATCCCTTCTTGCCATTCTGTCATCTAAGCGCCCATCTGGCTGTA2760.1304569775576185No Hit
CCAACCGGGAGTGGCTTCCAGACTAAATAAAAACAGAACATTTTATCCAAAT2750.1299843073490764No Hit
TGTAATGGGATTATACTTAAAGAAATATTTCATCGTCCAATTATATTTTAAA2750.1299843073490764No Hit
CTTTGTGGGAATACCATCAGTTACAAACTTTGGGACATTACTTAACCTCTCT2730.1290389669319922No Hit
CGCGATGGGCGATCTGGTCTCGGGGAATTGGATTTTTATAAAAACCTCTGAA2710.12809362651490802No Hit
GTGAACGGAGGGATGAAATTGCACTATAGCCACAGCGTTCTGTTTCTTAAAA2700.1276209563063659No Hit
AAAGACGGGATTCCTGGTGTGTTTTGGAGGGATAAATCTTGGGAGACCCTTT2700.1276209563063659No Hit
TTCTTTGGGATAGTAGATTTAAATTATTTTAGACTTCTTGTTTGCAGTTCAT2700.1276209563063659No Hit
CCAATAGGGAAGACATTTCAGTTAAGCTCATAAAATTTCATTGTTTTCATTT2700.1276209563063659No Hit
GGCGCAGGGAGTGTCTCTTACTGTACATTTTCTCCCTAATTCCTATGGCATT2690.12714828609782383No Hit
GCTATCGGGCACACACACAGAAGAAACCCTCAGAATACGCTTAACCAAGGAT2680.12667561588928172No Hit
CAATCAGGGCAACTGAAAACTTAGGTTGTTTCTAAATTAACTATTGTCAAAC2680.12667561588928172No Hit
TGAAATGGGGGGGATAAAAAGATTAAGAGATTTAGCTAGTGATACACAAGGA2670.12620294568073964No Hit
GCTGCATGTTTCCAGAATTTTAAGCTATTATCACTTAAAGGTTATTACACTC2670.12620294568073964No Hit
GCTGATGGGAGCCTTCCAAAGCTCAGGGATTACAGACATGAGCCACAGCACC2620.12383959463802915No Hit
GAATTGGGGCAAATTAATTGTGTGAACTTGGAAGCACACCAATCTTACTTTG2610.12336692442948705No Hit
GTAGTCGGGTGCAAAAAAATAAATAATGGGAAGAAAGGGCTGTATAATGAAA2610.12336692442948705No Hit
TGCAGTGGGGTCACCAAAACTAGGACATTTTTGAGAGTAAAAAGAGGCAATC2600.12289425422094497No Hit
TTTATGGGGAAAAATGCATTTTCCAAACAACTTATACATGCTTTTTATGACT2590.12242158401240286No Hit
CTAGCGGGGAGATTCAGTCAGTCTTCTATGGTTAATAAAATCTATAGTGAAC2580.12194891380386078No Hit
TTGTAAGGGCGATCTATGCGTGGGACAATTAAAATACTCATAGTAAATCATT2580.12194891380386078No Hit
TATCTAGGGCAAGAACTGGAAGGAACTCCCTGCTGTTTTAGAAAAAGAGAAA2570.12147624359531868No Hit
CTCCATGGGGAATTTCTCCATTCATATTTATAACCATTCTAGTTTTATCTTC2570.12147624359531868No Hit
CTTGCAGGGGTAAATGAACTTTAATCATGGCTGTTAATTGTAAAACATGACT2560.12100357338677657No Hit
TGATGCGGGGATGCAAAATAAAACAACCCTCAGTTCATACCTAGCTCCCTGT2560.12100357338677657No Hit
TAATATGGGATAATAAAAAAGCATTTCTAGCTACAAAATAACACAAGGAACA2550.12053090317823449No Hit
GAATCCGGGATGTGAATCAACAATGAATAGTCTCATTTTGGACATGGAAAAA2550.12053090317823449No Hit
GGGCCAGGGAACTGGACACACTTGGAAAATGCCTACATCATAAACCTAAAGA2540.12005823296969238No Hit
ACAGTCGGGTAAATCGAAAAATGTTGATGGCTTTCTTGTGTCTTTATCAAAT2530.11958556276115029No Hit
GCCACTGGGCCCAGCCAGAAGTGGGAACTTTCAGATGCTGCTGGAGTGAGTG2510.1186402223440661No Hit
CCAAATGGGGGAAGGGGCAAGACGGGTTCAGTTCGTCATGGGGCTGTTTGGA2510.1186402223440661No Hit
TTAGTTGGGAGACTTTGTGGACCATCATCCAATTGTTTTTTGGAACCTGGTA2500.118167552135524No Hit
CAGATGGGAGAAAATGAAATTAATATGAAGTCCAAATAACGATTGCATAAAT2490.1176948819269819No Hit
GCTATAGGGAGAAAGGGAACCAGGAGAAGTTGTGGACAGCCTGGTAGGCAAA2490.1176948819269819No Hit
GGCATTGGGAAATCAGATAATGAAAGTAAAAATGAAACAGTGTCTTCAAACT2480.11722221171843981No Hit
CATCTCGGGGAGCCTAGTTCTTAAAATATCTTCATTGACATAATAAAGTAGC2480.11722221171843981No Hit
ACACCAAGTTCCTCCCAGATTAAACTTCCCAGGTGTTCAGAATTACTTTTGC2470.1167495415098977No Hit
TAAGTGAGGAATTTCTAGAAAGTCAAAGAAATGCAAATTTAAGACAATAAGA2470.1167495415098977No Hit
TCCATTGGGAGATTTGAAAAGCTGACTTTTTAACATCTTGAGGCAACTGTAG2460.11627687130135562No Hit
GTGGTTGGGGTGTAGTCATAAATAGACAACCATCATTATTTAAATGGCATAA2450.11580420109281353No Hit
TGCGCAGGGGTAGAAATGAAAAATGAAAATTAAAGCTAAAGCCTTTTTATCT2440.11533153088427142No Hit
GATTATGGGTTACCCCAAAATACTTGGCTTTGAATGGTTCATCTTGAGTGTG2440.11533153088427142No Hit
TACTAAGGGGGAAGTAAAAAAAGGTCTAGATAACCTCTACAAGAAAGTTGAT2440.11533153088427142No Hit
CGTAACGGGATTCCCTGGGATGTTCTATTAATAATAAAGTGATAATAAATGA2430.11485886067572934No Hit
ACGAATGGGCAGAGTTTTCAACTCAATATGACACAGATAAATTCACTTCAGT2420.11438619046718723No Hit
GACATCGGGGAAGAAGAAGGGATGATGTGTGGTAGAGAAGATGAGGGTTAAC2410.11391352025864514No Hit
TTTACGGGGCACAAATAGAATAAATACATGAAAACTTGGATTAAGTCAGGCT2400.11344085005010303No Hit
ATCACCGGGGAGGACTCTTTGTCTTTACGGAGTTCATTATTCCAAGTGCATG2390.11296817984156095No Hit
GTCACGGGGCCAGAGCAGTCATCGTGATCAAAGTGCCGTCAATTATTAGGGC2390.11296817984156095No Hit
AACTCCGGGGAACTCCTTCATAAGCAAAATTATTTTTATGTGATCTCTAAGA2390.11296817984156095No Hit
ACTAACGGGACTCTATTTTGTGTAATGAAATTTTAGCTCTTGTCTATTGAAC2390.11296817984156095No Hit
AACAACGGGCAGGAACTGTGTTCTTGCCACGACTCCTCTTAGGAAAACGCCT2390.11296817984156095No Hit
TAAGTGGGGCGGTAGGAAACACAGTTATTCACAGTGGCCATCCCCAGCAGAA2390.11296817984156095No Hit
ATAGCAGGGATTGCAGATGTTTTTATAAATACTTTCATTAAAATGATGTAAA2380.11249550963301885No Hit
CACTTTGGGCTACATAAGTAATCTGCATATTTTTAATGCCTTCATTCATGAA2380.11249550963301885No Hit
ACACTGGGGGACTCAGACTTCTGAGGAATTTGGGTTTGTTCCCCTTTGTAGA2380.11249550963301885No Hit
TAGTAAGGGCAGATCATTGCTACTCAAAATGTGATCCATGGGCCAGCAGCAT2380.11249550963301885No Hit
ACAAATGGGACAACAGAAATGGATTCTCCTTTGATATAAATGTCAGTCCTTG2380.11249550963301885No Hit
ACTTTAGGGAACCACGGAGATAATCAAATTTCTTTGTCAATCATGTTTCTGA2370.11202283942447674No Hit
ATTGCCGGGAGTCCAGTTATGAGTAATGAGATTCAATCAGTAATTAAAAGTC2370.11202283942447674No Hit
GACACAGGGCTCTGATCATAATGGAATCGAGGTAGAAATCAATAACAGAATG2370.11202283942447674No Hit
GTTGCCGGGATTAAAGTAATAATGTGTTCCTTGAGGATAACTTGTCAAATGC2370.11202283942447674No Hit
TCTACCGGGTGAAAAAAAGGATGCAAAATAAAAAGCTGCCTAAGGTGAAAGT2360.11155016921593466No Hit
AACTGGGAACACACACCAACTGAGACCCAAATAATTTTTCTGCCATTGCTAT2350.11107749900739255No Hit
CAATTAGGGAAAGACTAAGGCGTATTATAGGAAGAGGGCTAGAAACCTAACT2340.11060482879885047No Hit
TTTACTGGGATATGTGTTGATTCTTGAAAACTGGGACGTGTATTTCCATTGG2340.11060482879885047No Hit
TACGGTTGGGTAGTGTAAGTGAATCGATGAGTAAATCATTAAACAAATTGAA2330.11013215859030838No Hit
GAGGCACCCCCTATTCCTGCTCCCCCAACTGGATCAGGTAGAACAACAAAAG2330.11013215859030838No Hit
GCCCAAGGGGTAACATTTCAGATAAATGGACACTTGCCATTTTCAGTTTTAA2320.10965948838176627No Hit
AGTTACGGGGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT2320.10965948838176627No Hit
AGCGCTGGGCAGATTTTCCTGGTACCCCACAAATAAAGCAAAAATATAGAAG2310.10918681817322419No Hit
GATGCTATCTTCAGGTACAGATACTCCTCAACTTACAATGGGTTTATCAGGA2310.10918681817322419No Hit
ATCCATGGGTATATTAGTAATTTATATAGATGTCAGCAATTAGGCAGATCAA2310.10918681817322419No Hit
CTTTAAGGGGTGAGGCTCCCGGGGTACTACGACAGCCCTCGAGGTGAAGGAT2310.10918681817322419No Hit
CTTATAGGGGAGCTGACCCAAGGGGCCTTCGAGGTGCCTTAGGCCGCTTGCC2310.10918681817322419No Hit
GAAGGCGGGTCATACAGCTAACTCACCTATCTCATCCATCAAACTTAGTTTC2310.10918681817322419No Hit
CCACTTGGGACTTGAAGCAAATTAAGATCTTTTAAGCCTGGACTAGCAAAGG2310.10918681817322419No Hit
GTACAAGGGTGGCATTGGAATTTTCCAGTACAATTTTCCATTATCTGCTCAA2300.10871414796468208No Hit
TACACCGGGGAGTGGCCGAGCCAAGGAGCTTCTGGGCCAGGGCCTGCTGCTG2300.10871414796468208No Hit
TCCATTGGGACAATTTACAGTGTACTTTGTTAAAAACACTGCTGAGGCTTCA2300.10871414796468208No Hit
AACCCAGGGCTTGATCCCAACAAAATGATTTACATTATAAATTCACATTATG2290.10824147775613999No Hit
AGAGGGGCAATGCAGCAAAATATAAGTTATAACATTTATACACCTAAAAAAC2290.10824147775613999No Hit
CGCAATGGGCAGTAAATCAGCCAGCGAATACCCGGAAGCAAGATGCACAGGC2280.10776880754759788No Hit
ATTATTGGGGAAGTAGGTGAACAAAGCCAGAGGACTTACATTTCCAGATACG2280.10776880754759788No Hit
GCATGTGGGAGATTACACTGGATGAATTCAAAGAAGCTGCAAAGAGCGACCC2280.10776880754759788No Hit
ATTAGGGGGATATGAATTTTTCTGTATCAATTCCCTCTTCAAGCCTGAATAA2270.1072961373390558No Hit
GCTGCTGGGAATTTAGGATGACATACAAGAACAATTCACTTACTAATAATTT2270.1072961373390558No Hit
ATCATAGGGGAGGAGATATAATAACATTCTTCTAGCTACATTTGTTGAAAAT2270.1072961373390558No Hit
CGTTCTGGGGAAGTGGACTGTTACAGGATCTGGCAAATCAAAAACATTATCA2260.1068234671305137No Hit
CTTATTGGGCATTTCTTGAAGGACATGGAGTGGAGATTGGATCATTTAACAA2260.1068234671305137No Hit
ATTCGGGGCAGCGCTTCCAGCAGCTGGTGCACCAGATGACTGAACTTTGTTG2250.1063507969219716No Hit
TCGCGAAGGGGGAGTGAGCAAGCGAGCAGAAAAGAGGTGGAGAGGGGGGGAA2250.1063507969219716No Hit
TGATATGGGGTAACTTGATTCCACAGGGTCAAATAAAGAAAACAAGAAATGG2250.1063507969219716No Hit
CAGCCAGGGACTGTAATCTTTGAAACAATCTTGTCGTTCATTTGTATTAATA2240.10587812671342951No Hit
GTAGGAGGACCACAATCAATGTCTTAGACACAGAGGCAAAGGATGCTTTGGA2230.1054054565048874No Hit
AAACCCGGGGTCTTGAACAATTTGCTGAATTTCTAGAAGATAATCGGCAATG2230.1054054565048874No Hit
GTCTTTGGGGGTCCCTGTGACCGGCTTGGCGGTTGGAGCGGCTGTCGCCATG2230.1054054565048874No Hit
CTTAGGGGTGGCTGTGATTGGAGTTCCGGATATGACATGGGGCCAGCGGGTC2230.1054054565048874No Hit
ACGTAGGGGACTTGCTCTTTTAGAGATGATTGAATTTTATTATTTTTCCCCA2230.1054054565048874No Hit
ATATGTGGGCTTGCAGAAATAATGAGGCCAGTAATGATAAGAGTGCCTAGTA2230.1054054565048874No Hit
ACAACAGGGGCATAATGTTTTTAGGGTTTATTCATGTAGCATGTACCTGTAC2230.1054054565048874No Hit
TAAGATGGGAGGTGATACACCCTTAACCAGAATGCTGCACTTTCTCTTTAAG2230.1054054565048874No Hit
GCATCTGGGGACCAATATAAGTATAGCTACTTGATATGGTTTGGCTGTGTCC2230.1054054565048874No Hit
TCTAATGGGATTGAGTGAAATATTTTCTTGGCAGATATTCCGTATCTGGTGG2220.10493278629634531No Hit
TATAGAGGGGAAAGATAGAGATTAATACAACTACTTAAAAAATATAGTCAAT2220.10493278629634531No Hit
AGTTGAGGGACATCATAGCCAGCAACAGCAAGAAACATAGCATAAGGTTTGT2210.10446011608780323No Hit
GCCCAAAGGGAAGCTGATTGCAGGGAAGATCATCCCAGCCATTGCCACGACC2210.10446011608780323No Hit
GCGTAAGGGTCCTCATTCATTCATCAGTGTCCAGTTACTTCTGACTTCTGGG2210.10446011608780323No Hit
TGTTACGGGGAACAGAGTCTAGAATTATGATAATCATATGGTTTCCGCGAGA2210.10446011608780323No Hit
CTTGGCGGGACCCAATGGTTGGTGTTGTTTAATTTCCATCTGCAGATGAACC2200.10398744587926112No Hit
ATACTAGGGCATTTTCCCACTTTTCTATTGAAATATTTTTATTTTTCTAAAT2190.10351477567071904No Hit
TATTGAGGGTATCACTCAGGGCTTTCGTACCTTCTAGGTAAAACTTCTTTCA2180.10304210546217693No Hit
ATTAAAGGGATTTCCTTGTTTAGTTTCAGAGAAACTTTTCACATTAGCATGT2180.10304210546217693No Hit
TTGTAGGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC2180.10304210546217693No Hit
TTTGCTGGGAACCAGATAACATCATGATGACAGGATTAAATCCACACAGATC2180.10304210546217693No Hit
GCGCGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2170.10256943525363484No Hit
CTGCAAGGGATACACATTTTCTAGCAGAATTTTTAAAATTTGTAAGTAGCAT2170.10256943525363484No Hit
CTTCCAGGGGCTTAATCTGGGGTATCAAGCCAATTTCCCTGTGATCTGGGTT2170.10256943525363484No Hit
CTGCGGGAGCCACTGTGTGTCATATTTGTTCTGAGAATAAAAAATAAATGGC2170.10256943525363484No Hit
AATGATGGGATTCTAATACAGTAAGATATTGGACACAAAATGGAGGTAACTT2170.10256943525363484No Hit
TTGCCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2170.10256943525363484No Hit
ACGCAAGGGACTGTCCGAGCACGAGAACGTGCTGGCTGAGTTCAGCAGCGGG2170.10256943525363484No Hit
AATGTTGGGATCTCCCTGGTGAGAATGCAGATGCCCTGGGCTCACAGGGCAA2170.10256943525363484No Hit
TTGGCGGGGCTCAAGTTTATGTGTGTAAACTGAGGCAGAAATGTTAATTATA2170.10256943525363484No Hit
CAGTAGATCCCAATTGCAAAACAGACCCTCCCTTTTTACTTTTTTTTATAGG2160.10209676504509273No Hit
CCTACGGGGACATAGAAAAGATTTTATTTTAAAATGAGTTGTAAAGCTTGTG2160.10209676504509273No Hit
AGACTGGGGTTGCCCACAAATGGTATTATAATGCTTGCTTAGTCAAAGAAGA2150.10162409483655065No Hit
ATACAGGGCCAGGCTAGTACCTTATTACTTTTACTTTGATTTATTCTCATCG2150.10162409483655065No Hit
ATCTATGGGGTTTCCTTTCTGCTTCTTGTTCTTCAATGTTATATATTGGTCT2150.10162409483655065No Hit
AAACAGGGCAGCCTCCATTTCCATTCCAGCCACAGAGCTTAAGAAAAGGGCT2150.10162409483655065No Hit
GTGCATGGGTCTCTTTTAATTTCAGCTATCATTTTTAGAGCCTTACAGTCAC2150.10162409483655065No Hit
ATATGGGGGGTATGTTAGCAATAAGTTTTAAGTGTTTGTTTTTCTTTTTTTG2150.10162409483655065No Hit
CGTTCAGGGAGGTCATTGTGAACTTAGAGTTTCAGTGGAGTGTTATGATCTG2140.10115142462800855No Hit
TTTACTGGGATGCTACAGAGAGTGAATACACTGGAATTCAGACACCGACTCT2140.10115142462800855No Hit
CGGTGTGGGGAAGAAGATGGGTACGTGGGTACATATAAAAGCCTATTTTTTC2140.10115142462800855No Hit
TTCTCTGGGTCTCAAGAGGTGAAATACAGAAAGCCTTTTTTTCTTGATCTTT2130.10067875441946644No Hit
ATGAGAGGGTAAGATAAAAGACAAATATAGGGTCCTGAAGGCAGCAAGAGAA2130.10067875441946644No Hit
TGATGCGGGGCCCCACAACACCCCTGTGGGTAAGAATGTCACTCATTTCATC2130.10067875441946644No Hit
CCTTGGGGCTCTTTCCCTAAGCAGCCTGAGGGTTGACTGGATTGGTGAGGCC2130.10067875441946644No Hit
TGCGCGGGAACTCGATGCTGAGTATCTACATGGATACATTAAATATATTTAT2130.10067875441946644No Hit
TTTAGAGGGCTGGCAAGAAATATGACTTGCTTTTTGCTGAGCTTAATTCAGA2120.10020608421092436No Hit
TGCAACGGGTAGGATTAGAGGCATGAGCCACTGCACCTCTTGCTTTTTTTAT2120.10020608421092436No Hit
CCCAGCTGTCTGCAGTGTGCTGTCATTTGCATCCTGCAGGTCTCAGTTCAAA2120.10020608421092436No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)