FastQCFastQC Report
Sat 31 Dec 2022
SRR5845407.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845407.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124015
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGC4720.38059912107406363No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGCCGTCT4300.34673225013103254Illumina PCR Primer Index 5 (95% over 21bp)
CTATTTGGGGTCACTAGCATTTTTGGTGTGAAAAATACAGAGCTGGCTGTCT1540.12417852679111396No Hit
GCTATGGGGGCTGAGGGCCAACTAATCAATATGGAAGGAGTGCTGGAGCTTG1530.12337217272104181No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAACTGTCTCTTATACAC1520.12256581865096965No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACCAGTAACTGTCTCTTA1510.12175946458089747No Hit
AAGCACGGGTCTTGCAGCGGGGAACATCAGAAGTATATCTACATGAAGAATT1490.12014675644075312No Hit
GTTAACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1490.12014675644075312No Hit
TCTTCAGGGCAAAATTCTCAGCTATACAAAAACTTGGAAAATATTTTCCTTA1440.11611498609039231No Hit
GCTGAGGCATGACAATTGCTTGAGCCCGGGAAGTAGAGGTTGCAGTGAGCCG1420.11450227795024796No Hit
TGAATGGGTCTGCCGATGGGAGTGAGTGTTCCAGATGAAAAAAATAGAGTGC1410.11369592388017577No Hit
ATCAGGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1410.11369592388017577No Hit
GGACCTGGGAGTTTCTGAGTGCAGCTTTTCCCATCCTTGGGATCAGCAGATA1390.11208321574003145No Hit
GTACTGGGGAGAATCCTTGGCTCCTTATGGCTTTTCTCCACCTCTTCTCCAG1350.10885779945974278No Hit
GGGTTAGGGCAGTTATCTTGAACATTCTGACGTGTAAAGGACCATAAGGTAC1320.10643873724952627No Hit
GAGGCAGGGGTAACTGCTCAAACACAGTAAACACACAGTTTTCCAGTTTTGC1320.10643873724952627No Hit
TAACCAGGGAACATGAATGCAATGAGTGCCTTATATCACATTGCCCAGAATG1320.10643873724952627No Hit
TGAATAGGGCTGCCCAGATTCAGAAGTCTGTCCTTTTGTGAGCTGGATTGTA1310.10563238317945411No Hit
TTAGTTGGGTAAGAGAATGTTGTGGAACTACTTCTGGCCAGACTTCTTAATT1290.10401967503930977No Hit
GGGGTTGGGATGGATGAATGGGAGGTGCGTTTGGAAGCAAGATGCCACTATT1290.10401967503930977No Hit
GCTACGGGTAGGGGTTAAGACTGATAACAACCTTTTACATTGTGACTGTGTT1290.10401967503930977No Hit
AGCTAGGGGACTCGTGTCTCGCTACCAGCTCCCCGCTGCCCTGCGCTCGGCG1290.10401967503930977No Hit
CCTTGTGGGCTTTGAATACATGAAGCATGGAGACCTGAATAAGTTCCTCAGG1280.10321332096923759No Hit
ACTACTGGGATTCTGAATAATGCTGTTATGAACATGGGAGTGTGGATATCAC1280.10321332096923759No Hit
AAAACTGGGACTGGACCAGATAGTAGACTGGTCCCTTGATCAGAAGCATCAG1280.10321332096923759No Hit
TGCACAGGGTGTTGCAAATGACAGGATCTCTTTCCTTTTTATGGCTGAATAG1270.10240696689916542No Hit
TTCGTAGGGTAATTAGAAAGCAGTTAGGGTCAGAGAGAGTTTTTGGGAAATA1270.10240696689916542No Hit
GAGAATGGGATTTCTCTGTAGCCAAAAGCTGGCAAACTTGACCCAGAGGGAA1270.10240696689916542No Hit
GGCACAGGGATAGAGATTAATACAACTACTTAAAAAATATAGTCAATAGGTT1260.10160061282909326No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)