FastQCFastQC Report
Sat 31 Dec 2022
SRR5845310.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845310.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22086
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTGTGGGCTGATAATGAATGCCAGCTTTCCTTGTGCTTTGATAACAAAGA5472.4766820610341393No Hit
GCATAAGGGAGCTCTCCGGAAGCTAGAAGCAGATGATTTTTTCCAAAGCTGC4662.109933894774971No Hit
TATTCTGGGAGAATACATCCCAGTAAGGGTCCTATGGAGAAAGACCTGTTGT4562.0646563433849496No Hit
TATGTAGGGCGGCGCCGACCAGGAAATTTCGATTTGCACACTGATTTGGCCC4061.8382685864348456No Hit
TGCAGTGGGGGGACAGAACCGTGTCCTCAGGAGCTAACACCTGGGCTCCATC3891.76129674907181No Hit
ACTTTAGGGTAAAATTTAAACAGATGGGCCTGAATCCAAACAAGGACACAAC3671.6616861360137642No Hit
GGTGCTGGGATACCCAGAAAATTTCATACTACTGTTTTCCAAAGTGAAGTTG3281.4851036855926831No Hit
TAGCTGGGATTACAGGTGTTTGCCACCATGCCCAGCTAATTTTTGCATTTTT3211.4534093996196686No Hit
AATCGTGGGATTTATGAATAACTGTTGATTTGTAAAGTCATTATGGACTTGG2971.3447432762836184No Hit
TTAGGAGGGGTGTAAAGGTGAACACTGTCACCTCAACCTGTTCCAGGTAAAC2831.2813547043375895No Hit
TCTTGTGGGAATGCCAAATTATTCTTCATAAGAGCTGTTGCCAAATCAGTGA2791.263243683781581No Hit
CAAAGGGGGGAAGAAGACACATTTCTAGATTATTTGTCCTTTTTATCCTCTC2751.2451326632255728No Hit
GTGACTGGGTGAGGGCTGGCTATTGAGAACTGGAAAGAGTAAAATGATAACT2681.213438377252558No Hit
CTTAAGGGGGTTAAATTTCTTGTACAAAATTGTTTATTTGCCTTTTCTTTGT2601.1772163361405414No Hit
AAATAGGGGGAGTCATCCTGAACTAGTTTGAAATTGTGAAGCTGCTTCTTGG2591.1726885810015395No Hit
TTATGAGGGTACAAATGAGCAAGGTATTTGTTGCAACTTACTACATAGCATA2581.1681608258625373No Hit
AAGCCAGGGCTCGGGCATGGGGGTCGATAATAATTAAGTCTTAATTAGTAAC2561.1591053155845332No Hit
CTCTCTGGGCTTGCAACCTAGTTCATGATAGCAGAGTACATGGGCATGCAGA2511.1364665398895226No Hit
ACGCAGGGGGATACCAAACCCTTATAAATCAAACTCACAAGTTAAAAAAATT2411.0911889884995019No Hit
GTAGCCGGGGAATTGATTGTTGTGTAGGTCTAAGATAAGGGTCTGATTTCAT2351.0640224576654895No Hit
TCTTGAGGGAACCGGCCTCCCATAAATTCCAATCACATTTCTTCCCTGCTTA2140.9689395997464456No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC2090.9463008240514353No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT2040.9236620483564248Illumina PCR Primer Index 5 (95% over 21bp)
CACAGCGGGAAGTGTTTTATTAATAATTAAATGTGATAATACTTAATGAGAA1960.8874400072444083No Hit
ATCGCGGGGGGAACTAAGCAGTGAACACACTGGGGGAAACGTTTGCTCCAGA1910.8648012315493978No Hit
ATCACCGGGGCACCACTGCCCTCCAGCCTAGGTGACAGAATGAGACTCTATC1840.8331069455763833No Hit
CTGGCTGGGATTACAGGTGCGTGCCACCACGCCTGGCTAATTTTTGTATTTT1790.8104681698813728No Hit
GTACATGGGGAAAGTGGCAGTCCCAGGACCTACCAGGTAAAGAGATCAGGAC1760.7968849044643666No Hit
ACTTGGGGGAAGAAATTGGCAATATCGTATAAAAATCTGCTCATATTAGAAC1730.7833016390473603No Hit
CTGGCTGGAAGTCCTGATACTGAATCTCCAGTGTTAGTGAATGACTATGAAG1670.7561351082133478No Hit
CAGACTGGGATTCATAAGTCATAGAAAATAAATACATCATATAGTTGTATGG1640.7425518427963416No Hit
GTGATGGGGAAGACAATGGAGGGTTTTTTGTTTGTTTGTTTTTACTATAACT1610.7289685773793353No Hit
GATCGCGGGATTCTAATCTATGCTTGTGATACAGATTTCTTTATATCTTCTT1540.6972742914063208No Hit
TCTAGAGGGTCTGGAATTTCAGTAACAATATGAAATTAACATCATTATCATT1450.656524495155302No Hit
ACGTTAGGGCAGACATCACATGTTCTGACTTATTTATTGGAGCTAAAGATGA1370.6203024540432853No Hit
TCAGGTGGGACCCCCAGGCGAGCATCTGACAGCCTGGGTCGGCACCCACACC1310.5931359232092729No Hit
CAAGCCGGGAAAGGAAAGAAAGCAGAATCCAAAACATCAGAATGAATTGAGA1270.5750249026532644No Hit
AACGCCGGGAGGACAGATGGAGCCTCCCTTTCCTCATCTACTCTGTGGGTGG1260.5704971475142625No Hit
GCACAAGGGAAGGGGATTGAATCCATAATCATAAATCTGTCAACAGAGAAGA1210.547858371819252No Hit
TGAATGGGCACCAATCACCTCAGCCCTCTACCCTACAGATTTCTAAATCATA1150.5206918409852396No Hit
TAGCAGGGGCTGGGAGGCCGAGCTTGCAGTGAGCTGAGATCGCGCCACTGCA1150.5206918409852396No Hit
TCAGGCGGGATCTGCCAGTGCCTTGGTTTATAGGCCATTTAGTTTATGATAT1120.5071085755682333No Hit
GCAAGGGGGCGATCCTATCATGACTTAACATCAAGCGCGCAGTTCAAAACTA1090.493525310151227No Hit
ACAACGGGATGGTTAGTATTGGCTAAGAAAGACTATAAGGTATACATGTTAT1040.4708865344562166No Hit
CTTCTCGGGAAGGAAGGAGTGGTTAGTAAACTGCACTGTTTCTCTGATAATG1030.46635877931721453No Hit
ACTGAAGGGTGGGGACAATGTGATCATCCTTGGAGAGAGATGTGAGCACCAA930.42108122792719366No Hit
GGGCCTGGGAGGCGGAGGTTGCAGTGAGCTGAGATTACACCACTGCACTCCT880.3984424522321833No Hit
CGCAGTGGGGCCCACTGGCTGCTCTGAAAAGCCATCTTTGCATTGTTCCTCA850.384859186815177No Hit
ACGACAGGGCTGACATTAAAATTGTCTGATTTTGTCCGTTTCTTAAAATTAT840.38033143167617495No Hit
CACTCCGGGATTGTATATGAGGTTGCAGTGGCTTAAAAAGGAAGGACTTTTG830.3758036765371729No Hit
TCCCACGGGCTCTACTAAACATACAAAAAATTAGCCAGGTGTGGTGGCACCC770.3486371457031604No Hit
GGCCAGGGTGGGCGGATTACCTCGGGTCAGGAGTTCGAGACCAGCCTGGTCA740.3350538802861541No Hit
TATTATGGGGGGAGTTAGAGGCTGTTCCAACCATCCTTAGTTTGGGAGAGTG730.3305261251471521No Hit
TCCTACGGGAAGAACTGAAATTTTTAATTAAAATTTATTTCCCCTATTAGGC730.3305261251471521No Hit
AATATGGGGATGGGAGTAGAAAACAACAAAGAATGGAATGGACTCTTAAAAC670.3033595943131395No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTG660.29883183917413747No Hit
GGAGCTAGAAATACCATTTGACCCAGCCATCCCATTACTGGGTATATACCCA650.29430408403513536No Hit
CATAAAGGGCAAGTAGTACCATTTTAAATGTTATTGCAATTTGGAATCTGAA620.28072081861812914No Hit
GCTCCCGGGTTCAGCTGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACT620.28072081861812914No Hit
GACAGAGGGTTCTGTATACATGTAGCTTTGCCAGATATGTACTTAGTAATAT620.28072081861812914No Hit
AGTTAGGGGGAAAAAAAGAACCATTTGGATACATAGGTATGGTCTGAGCTAT580.2626097980621208No Hit
AATAATGGGGATCACCAAAGAGGAAGCCTCTGGAAGTTCTGTCACAGCTGAG570.2580820429231187No Hit
AGAGATGGGCAGGCCAAGAGTTTGAGATTAGCCCAGGCAACATGGCAAGACC560.25355428778411665No Hit
ATGCGGGGGGATTCGTGGGCATGATCCTGATTCTCATCTTCGCCGAGGTGCT550.24902653264511454No Hit
TTTACAGGGATATCATAGATATGAAGACATAAAGCAGCAGGTTTGGGGTTTT550.24902653264511454No Hit
AAATGGGGGGGAGAAGGGAAAGAAATAAAAGAAGAATACATAGTATATTGAA540.24449877750611246No Hit
GAATAGGGGTTAGCAAGTGAACTTTATAATATTTTAGACAGCTTATATTTAC520.2354432672281083No Hit
TGTTACGGGAGTGTATGGGATATTTATAAATTCAAAGAATCTTTTTTTTATT480.21733224667209997No Hit
TGTAATGGGGTTGCAGTGAGCTGAGATTGCACCACTGCACTGCAGCCTGGGC460.20827673639409583No Hit
AAGTAGGGGACATCCAGATGGCCTGAAGCAAATGAAGATCCACAAAAGAAGT440.19922122611609164No Hit
ATATAAGGGGGGTTCTTTGTATTGGCACTTTGCACCAGAAACATATCATTAT430.19469347097708956No Hit
CAGCACTTTGGGAGGCCTAGGCAGGCGGATCACCTGACGTTGGGAGTTCGAG430.19469347097708956No Hit
TTAGCTGGGATACATGTGTGTATATATGTGTATATATACATACACTTGTATA430.19469347097708956No Hit
TATAGGGTGGGAGGTGAAACGGTACGATGGTCTCAACACCTTTCTTCTGAAC410.1856379606990854No Hit
AATGCAGGGAATGCAGGAGGACAGTGAAGCAAAGTCAATAGATTTTGTAGAA390.17658245042108123No Hit
GCTAGAGGGGGGGTGATACAAATGTTCTGATAGATAGAGGTGGTGGCTGCAT370.16752694014307706No Hit
TTTACTGGGGGGTGATGATTAGAGGAAAGGGTTTTTCTGGTTTGTATGTTGG370.16752694014307706No Hit
TGGGCAGGGAAAAGGGTCAGGGTTCATCAGGTGAACTCAACACTGGGATGAG360.16299918500407498No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGAT330.14941591958706874No Hit
TGCCTTGGGGTCCACATAATTGTTGTACAAACCAGAGCTTTTTAAAGTGAAA320.14488816444806665No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCTTATAC320.14488816444806665No Hit
CAGTCAGGGATACCATAGTTTATTCAACCAATTGCACATAATGTGGATCTCT320.14488816444806665No Hit
ATAGAGGGGGCCAAACAGATTCACTACTCTTACTGATTCTTCACTGAGCTTT320.14488816444806665No Hit
TGAGGCGGGAAATCAATAAAGTGCATTTTCAAAAGAGCTACAGCAAATTTAT320.14488816444806665No Hit
CTTCATGGGATAGAGATAGAGAATAAAACAGTAATTACTAAGGTCAGGATAA310.14036040930906457No Hit
GATTTTGGGGTACATTTTGATTGCCAGTATCAGTTTGGAAAAATGTTTAAAC300.1358326541700625No Hit
ACAAAGGGGTACATATGATATTATATATTTAGAAAATCAAAGTGAATTAACT290.1313048990310604No Hit
GTTTTGGGGCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCT290.1313048990310604No Hit
AACCTGGGATAGTGCAAAATGAAGATTTCAAAAACCCAGTCAAATATTTTAG290.1313048990310604No Hit
AGATAAGGGCCACAAGGACTGAACACCCCTACTCCAATTTAGACCTGCGGAT280.12677714389205832No Hit
GAGCAGGGGATTTGTTTCCTTTGGATGCCGTATCAAATTACCACAAGCTGAG280.12677714389205832No Hit
TTTTAAGGGGGGATAGATAAATCAAGCTAATTATACATATGTATTACCTCAC280.12677714389205832No Hit
GGCCTGACTTGGGGGAACGATGTGTCTGTATTTCATGTGGCTGTAGATCCCA270.12224938875305623No Hit
GAGGCAGGAGAATTGCTTGAACCTGGGAGGCGGAGGTTGCAGTGAGCCGAGA260.11772163361405415No Hit
GCCCACCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTAAGCCACCGTGCC250.11319387847505208No Hit
GTAGAGGGGTGTTTGACACAGGGTATCTAGGAGGGGCGGGGAAGACATGGAT250.11319387847505208No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCT250.11319387847505208No Hit
TTGCAGGGACATGGATGCATCTGGAAACCATCATTCTGAGCAAACTGTCGCA250.11319387847505208No Hit
TTAAAGGGGCACTTTTGAAGTGGAATTAGTGGATATTGATTGAAATAGCAGT250.11319387847505208No Hit
ATACAAGGGCTCCCACCAACAGTGTAAAACCATTCCTATTTCTCCACAGCCT240.10866612333604998No Hit
ATCAAGGGATAGCTGGAATTAAGTGTTGTCTTGGAGCTGTTGTACATTTAAG240.10866612333604998No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGA230.10413836819704791No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)