FastQCFastQC Report
Sat 31 Dec 2022
SRR5845292.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845292.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24597
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC900.3658982802780827No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT670.2723909419847949Illumina PCR Primer Index 5 (95% over 21bp)
GCGCAGGGATTGAAATCTGCAGTGAGATATAACTTTCTAGGAACAACAAAAT500.20327682237671263No Hit
CAATTGGGGCAAGAATGAATTAGAGTGAAGGAAAGCTGTGTGACACCTGGCA490.19921128592917833No Hit
AAAAATGGGGGGTAAGTAAGGAAGTCTTATAAATCTATTTTAGCCACCTAAC440.17888360369150708No Hit
GAACATGGGATTCCATGCTTTTCTGTGTTTATAAATCTAGGGTTACATGATG430.17481806724397284No Hit
TTTTGAGGGATCCATATAGAAACTTAAGCTCTTGACCTGAATTTTTCTGGCT430.17481806724397284No Hit
CGTATGGGATGCTTTAAAGTCCTGCAAGACTCAGGTCAAGTGCTTCCATAGT420.1707525307964386No Hit
CTTAATGGGATCATTGTGGTTTTCATTTGCACTTCCCAATGATTAGTGATGT420.1707525307964386No Hit
CGCATTGGGATCTCAGCTACTTTTTTCAATTTTATTATTTCCATTTGGTTCT410.16668699434890435No Hit
GTAACCGGGGGGACACACATACTTTTAGAAGTTAATAGAAGTCTTGAAGTCT410.16668699434890435No Hit
GACTTTGGGAATAAAAAGCTGCCTAAGGTGAAAGTTAGAAATTGTAGACTTT410.16668699434890435No Hit
ATTCGAGGGGGGTCTGTTGCTCTTATTGGCTTCCAAATGTGCATGGCAAAGA410.16668699434890435No Hit
CGTAGCGGGACCACATACAATTTTTTACACAAAATTATTTCCTTCTAAATTC410.16668699434890435No Hit
CTCAAAAGAAGACATTTATGCAGCCAACAGACACATGAAAAAATGCTCATCA390.15855592145383582No Hit
AAACAGGGGAACCTTCCTTAAGTACAATTTAACTTCAAGAAAACAAAATTTG390.15855592145383582No Hit
AGACATGGGAGATCTTTTAAGACTTACACCCCAGAGATTTAAGAAGAAAACC380.15449038500630158No Hit
GACTATGGGACCCACATGCAGACACACACACACACACACACACACACACACA380.15449038500630158No Hit
AGGTCAGGGGTAAAATAAAAAGTAGAAGCAGAGGCAAGATTATAAAAGAACA380.15449038500630158No Hit
AACATGGGGAGAGTGTGGTCAGGCGGCTCGGACTGAGCAGGACTTTCCTTAT380.15449038500630158No Hit
AGGTTTGGGAGTGAGTGTTGATGAGGATGTGGAGAAAAGGGAACCCTGTGTG370.15042484855876734No Hit
GCTCTTGGGGAGCCACCACACCCAGCCTCAAAATATGGCCCTTTTTAAAGAG360.14635931211123307No Hit
GCTGTTGGGTAAGATGTATATTAAAGTAAAGCTACATTTTTTTACTTCATTA360.14635931211123307No Hit
AAGTTCGGGGGCAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAG350.14229377566369883No Hit
TTCATAGGGACAGGAAAACAAGGTGAAATGAGAGAAATGCAACAGCTTTCAG350.14229377566369883No Hit
AAGCATGGGATGAGACAATTGAGTTTTACTCTTTGGAAGACTAAACCAATCA350.14229377566369883No Hit
TGCTGGGATTGTATTCTACATATATAAATATGTATTTTTATTTTTTTACTTG350.14229377566369883No Hit
TAAAAAGGGAAGTAGAGATAGTTTTTGGGTATTATTCTGTTTTCCTAATGAT350.14229377566369883No Hit
TCTACAGGGGTATTTACATATATTTTATATTAAGTTTCACATGAAATTATTC340.13822823921616456No Hit
TAACTAGGGCGATCTAACAAAGGGCGATCTAAAGCTGGGAGTGGTGTCTATC340.13822823921616456No Hit
AACTAAGGGGACTTGAAATGTTAATGGAGAGATATTCACAACTCCAGTGTAA340.13822823921616456No Hit
AGTAGCGGGATGCCTGGCTAATTTTCTTTCTATTTTTTGTAGAGACAGGGTC340.13822823921616456No Hit
CATTGAGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA340.13822823921616456No Hit
TTTTCAGGGATTTAGTAGGAATGTAAAGATACAAGTAGACAGAAAGTCTTCT340.13822823921616456No Hit
TGCAGCTGGGATAGTAAACCCCTGTTTTACAGATGCAGGGGACGTAACTTAG330.13416270276863032No Hit
TATGCAGGGACGGTTAAATTCATGCACATTTGTTCTTGTAGTTTCTAAAAAT330.13416270276863032No Hit
ATATCGAGGGAAACAAAATAAAGGCAATAGTGTATGTAAAAATGAAAGCACT330.13416270276863032No Hit
GGAAGTGGGAATCAAAATGGCAAATTATAAGATATTTTAAATTGGATAATAG330.13416270276863032No Hit
AATACAGGGATTCCTAACTTTATAAAGTTAGAGCAAAAAGATTTGCGGATAG320.13009716632109608No Hit
TAACAGGGGATCTGCCTTTTGTTGTACCCTAGGGCATCCTTTCTACCAGAGT320.13009716632109608No Hit
GCTTTAGGGACTTCTGGGGAAGATGAGTTAGTCTGAAAGGACCTGGTCTTTA320.13009716632109608No Hit
TATTTTGGGACCATGCTTTGGATGCTCTAGCATTTAGATATTGAGAAAATGA320.13009716632109608No Hit
TACAGGAGGGGCAAATCAAAACCACAATGAGAGACCACACCCATGAGGATGG320.13009716632109608No Hit
TATAATGGGGACACACAACTTATTCTTCTGATGTTCAATATATGCTGATATT310.1260316298735618No Hit
TATAATGGGCTGGCAAAGAAGTTGCTGTTGGGGACTCGGAAGGCCGTATTTG310.1260316298735618No Hit
TTGGCTGGGATTTTAATAAATTTAAAATTAACTATGAAATGTCATATGAATG310.1260316298735618No Hit
AGTCTCGGGGGGGGAAATTATTCAATCTAGTAGTTTCAAACTTGTCAGCATT310.1260316298735618No Hit
TGAACAGGGAACTCTACAGACCAAACCATTTGTATCTGGCATCACTTACTAA310.1260316298735618No Hit
AACGTAGGGATTTCTTGAAAGGCAGATTTACTGGCAACACATTTTCTCAATT300.12196609342602757No Hit
ATCTAAGGGAGTCTGTGGGGAGTCCCATATTTTTCTTACCTTAGATATTTAT300.12196609342602757No Hit
TTCATGGGGTTATGTTTATGAACAAACAAGGTGAAGGGTTCAGTATAAGTTG300.12196609342602757No Hit
AATACAGGGAGAGTCTGCGCTGTGACCTTCTGTTGGGCGCGTGTCTGTTGGT300.12196609342602757No Hit
GAAATTGGGATAATAAACAGTAAACGTTGAGGTTCATCAATAAAAGCACAGA300.12196609342602757No Hit
TGCGAGGGATTGATACCATGGGGAATCATAGAAATAAAGTTGAATATCATTG300.12196609342602757No Hit
TACGCAGGGACGACAGGTTTGGTTTTTAAATTTTCCAACCAAGTGGAAAGGC300.12196609342602757No Hit
TAGTCGGGGATTTGGTATGCTGTTAGGAGACACCATTATTTTGGATAATCTG300.12196609342602757No Hit
TTACAGGGGAATACTGATATTTTCCCCACCTCTCAATTGTTTTTAATTTTTA300.12196609342602757No Hit
TGCGCTGGGGGATGGACAAGTGTGTGTGCCTATTGACCGCCTTTTCCGAGCG300.12196609342602757No Hit
AAGCTGGGGCAGTGTTATAAATTCAACATAGAGGCTGACAACCTAAATTCAT290.1179005569784933No Hit
AACTAAGGGATTCTATTATAATTACAAGATGATTTTGTAAGCTTCACGGTAA290.1179005569784933No Hit
GATCAGGGGTAATATGTGTGATATGGGCTACTTCAGGACTCTCTGTTTTCTC290.1179005569784933No Hit
GTGACAGGGGGTAAGGTTAGTAGTGATGCCCTCTATTTATTACCAGATTTTA290.1179005569784933No Hit
CTAAACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA290.1179005569784933No Hit
AGATGGGGGATGGGGACAGTTTGGAAAATTGACATTCATGTGACTTAGAGTC290.1179005569784933No Hit
AAGGCTGGGCATACATTGAGATAATATATTGCAGCTAAAGATAGGATAGGGA290.1179005569784933No Hit
AACCAAGGGCAGATGGTGAAAGAAAACCTGAGGAAAGTGGGACAACCAGTCA290.1179005569784933No Hit
AATAGTGGGCTCCTGGGTTCAAGCAATTCTCCCACCTCAACCCCCAAGTAGC280.11383502053095905No Hit
TCATCGGGGGACACCACTGCAGTGTGAGCAAGGTGAATCCGTGTGCCATGCA280.11383502053095905No Hit
ACACATGGGGAGCATTTATTCAACAAATATTTACTGAGAGTCTACCGTATGA280.11383502053095905No Hit
TAGACTGGGGATAAAAACTGAAGCGCCAGCAGAATTCTATCACCCAGGGATT280.11383502053095905No Hit
GGCGCTGGGCTTGTCTGAAGCTGATGGAAGATGGAACGGTTCAGTCCTCAGT280.11383502053095905No Hit
TCGTCAGGGGATTGGAGCCTCAGGACAAAGAGCAGATGGTGAGAATCTGTGT280.11383502053095905No Hit
TTGCCGTGGGGAGAAAGGAACACAATACTGCTTGAAAATAGTACTAATGTAT280.11383502053095905No Hit
AAAGTCGGGATTGGAATGCATCTTTATGAATGTAAAGCAGTTCCTTATTTGA280.11383502053095905No Hit
AATTGGGGCTTCACAGGGACTGTGTCATCATCAGCTTAGGAAATAAAGACCT280.11383502053095905No Hit
GCACAGGGGGGGCGAGCGACCGAGCGCCGCGACGCGGAAGTGAGGTGCGTGC280.11383502053095905No Hit
GCATTGGGGGAAGCAGGGGGGCAATGCAAGTGCAACTAGCCAGAAAAACCAC270.10976948408342481No Hit
CATTCCGGGAAATAGACATGAGTGGCCCAAAGCAACAAATTAATACACTTTT270.10976948408342481No Hit
ACGCTTGGGGGATACCAAGTGTTTATATGGGTAGCTTGACTTGAGAGATTGA270.10976948408342481No Hit
GAATGAGGGAGATGAAATCGAGTGGCTATTGATCCGTGATGTTTCTAGATTT270.10976948408342481No Hit
CAAAGGGGGTTTAATGGATTGGCATACAGCCTAATGGAGATTAAATACAGAT270.10976948408342481No Hit
GGCATAGGGAGCCAAATTCCTCTTGAGAAGCAAGAGATGTTACAGAATGTTA270.10976948408342481No Hit
CTTGCAGGGATTGTGCTTTTCACAAGTGCTTGTAACTGTACCCTTGTTTTAC270.10976948408342481No Hit
ATTCATGGGAGTTTATTAAGATAGCTATGGACAGTGTAGGGGCTTAGTCTTG270.10976948408342481No Hit
ACTGGGGATTTTTCATCCATTATCATGATGTGCACCTTGCAGGTGTGAGGTT270.10976948408342481No Hit
CACAATGGGGCGGGCATTGGTGATATCCTGGAAGAAATTATCCGACAGATGA270.10976948408342481No Hit
CACTGGGGATTTCTGCATGGTCAAGTCCTCCTCATTCTTCATATTTCAGCAC270.10976948408342481No Hit
AACTTAGGGACGCTCTTCCGATCTTTTACTGGGATATTCCTTTGGGAATATA270.10976948408342481No Hit
CTTTGGGCAGCAAATTGGTCCCAGAATGTGCTTCGTATATTTTCCTCTTGAC270.10976948408342481No Hit
GTTTATGGGCGACGCTCTTTCGATCTAAAACCGGGGATCTTAAGGCCGGGAT270.10976948408342481No Hit
CAACAGGGATAGAATGTAAGCAGAAATAATGTCATAAGCAATTTTATATGAA260.10570394763589055No Hit
CAGCTAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA260.10570394763589055No Hit
CTTTTGAGGGGAGTCTAATTTCCAGTAAGGTATAGAAATGTTACTCTTATAT260.10570394763589055No Hit
AATTCAGGGTAAGCACAGTATTTATTGCATCAAATATGTACCACAAGAAATG260.10570394763589055No Hit
AGATGTGGGAGAACAAACAGCTTTTCCATTTTGCTCTGCTGCAGTAAATTGC260.10570394763589055No Hit
AAAGTAGGGGATCATCTGGCTGAGCAACTGAATTACAAAAGAGAATTACTTC260.10570394763589055No Hit
CTAGCTGGGGTTGGTTGGGAACTTCACTTGTTCTGGATGTCGCTCATTTTTT260.10570394763589055No Hit
AATTTAGGGTGGGGAGAAAAATCTTCAGTGTTTTACCTTGCTAACATTTTAC260.10570394763589055No Hit
TAGCTAGGGATTCTTAATCGCATATAAAAAGAAATCAGAGATTAATTTTATT260.10570394763589055No Hit
GCTAGGGACCTTAGAAGTCTCCAGAGTTATTTTCCTAAATTAAGAAGAACTG260.10570394763589055No Hit
TGCAATGGGAATATGCTTTATTTCAACAAATAATTTTGTGCAATTTATAATA260.10570394763589055No Hit
GATATAGGGGTTTTCAATTTAATTAAATACTTCTTTTGATTTGCTTCACTTG260.10570394763589055No Hit
TTACGTGGGGAAGATGGAGGAGGAGATGGGATCAGAAACATAGCTGGATAAA260.10570394763589055No Hit
GGTGCAGGGCAATAGAGAGATAGAGATAGATATACATACATGTAAATACACA260.10570394763589055No Hit
ATTGTAGGGATGCTGTAAAGCTTGTAGCCTGAGAATACTATAGATTTCACCA260.10570394763589055No Hit
TTAAGGCGGGGGAGATGGAGGAAAGAATGTTGTGAGCTTTTTCATCAATTAT260.10570394763589055No Hit
AATGTTGGGACAGAAACCTAGCCATTCAAAATCTGTTTAAAGGACAAAGTTT260.10570394763589055No Hit
TAGTACGGGTGTCTTTTTCAATGAAGCTTTGCAGATCACCATGTGGTTGAAG260.10570394763589055No Hit
GGCAACGGGATCTAAGGAAGGGCTATGAAACTGGGCCCATCTGTATACAGGC250.10163841118835631No Hit
TCTGGAGGTCTAGAGGGGGGTGTAGGTATTTTGTCATGTATTTAAGAAAAAG250.10163841118835631No Hit
CTAGTAGGGCAGCCTCCACCTGGAAGAGAGCTAGGGCCGGGCAGGCCGGGCA250.10163841118835631No Hit
TGGTATGGGGAAGGTGTGTATGTTTAACCATCTTGGTTTCTGCGAGAGAACA250.10163841118835631No Hit
GAGTCAGGGGCTAGTAGCGGGCAAGATGAAATAATAGATAAAAGCTATACTC250.10163841118835631No Hit
TTGTCGGGACTGCGGACCTCCAAAATTTTACAAAGGACTGCACAGTGAGTAT250.10163841118835631No Hit
TGGTTGGGGTGCAATAGACAGAATAGATGCTAGATTCAGACACAAAGATGGT250.10163841118835631No Hit
GCTATTGGGGTGGGGAGTTTGTATTGACTGTGGTCAAGCTCATAGATTCTTT250.10163841118835631No Hit
AAGTTTGGGTGGCCAGACCCTGCTGCTCACTCAGCTGCCCTGGGGGCTGCTT250.10163841118835631No Hit
TGCTCACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC250.10163841118835631No Hit
CAGCTTGGGATTCTTCCTCCCTTAAATACCTGCGCTTGTACTCTCTAAGATA250.10163841118835631No Hit
TTCCATGGGAGAGCTGAGTGTCACTATTGCAGTTGGAGCATCACTGCTGTTT250.10163841118835631No Hit
GTCGACGGGATTTTCAAGGATGTTTTTCTTTATTTTTGTTAATATTAAAAAG250.10163841118835631No Hit
ACAATTGGGACAGTTACCCTAGTGTGCAAAGAACGTCAGGACTTATACATAA250.10163841118835631No Hit
ATATGTGGGCATCAGAGAATAAAAAAAGTATTATAAAAATGAGTTAAAATCA250.10163841118835631No Hit
GTCGAGGGGAGACGAGTTAAGTCCTGGTCTGCGTGGAGGTCGACGACTCCGT250.10163841118835631No Hit
GTGCAGAGGGGAATATCAGAAGGCTATTACCACTGTTTGACCACAAAGGTAA250.10163841118835631No Hit
ATTGAAGGGGTTGATGGATAAAGATGTCTTATTCCAATACACCTTAACACAT250.10163841118835631No Hit
TTATTTGGGGGCTGTGGAAATCAAGAATACTTGCCATGTGCGAAGTTGTTTG250.10163841118835631No Hit
CGCTAAGGGAAGGTGTACAGCCTATTTTTTTTCTAGACTTACACTGATTTTT250.10163841118835631No Hit
TTGCCTGGGATGAGGATTAGGCGAACACTTTGTAATACTTTGCCCAAGAAAA250.10163841118835631No Hit
GTCGTTCGGGGATAAACATGCTGTTAATGGCATGGACTCTGGTGTCAGTCTG250.10163841118835631No Hit
TCACACGGGCATCAGAAATTCAACACTTTATTATAAAACGGGCTTCGTGTTA250.10163841118835631No Hit
CACATTGGGATAGGAGATGATCTTCAGAGAAAACACTTGAAATTGCTACTTT250.10163841118835631No Hit
AATATGGGGGGTTTGCATTTTAACAATTAGGATGGTTTATTGATGTGAATTT250.10163841118835631No Hit
TTATTGGGGATTTTGACAAAATTCAATACCCTTTCATGACAAAAATCACTGA250.10163841118835631No Hit
GGGCCGGGGATCTCAAGAAAAGAAGAAGTTCACAGATGAATTTCTAAAATCT250.10163841118835631No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)