FastQCFastQC Report
Sat 31 Dec 2022
SRR5845277.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845277.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31970
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT5221.6327807319361902Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC3791.1854863934939004No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCTTATAC730.2283390678761339No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTG640.20018767594619954No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGT480.15014075695964968No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCCTGTCTCT480.15014075695964968No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGA440.13762902721301218No Hit
GTATAGGGGATTTCTTCAGCTACTGATTTTTAGAACAACACAGGGCTGCGGA440.13762902721301218No Hit
TCGTTGGGGGGCCGTGGGGCTGGGCCCCAGCGTGCCTGTGCCCAGCAGGTCC420.13137316233969346No Hit
CAAGCAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA380.11886143259305598No Hit
ATTATGGGGAAAGAATAAATGCGTGCATTCCATAGGATGCAAGAAAAGGAGT380.11886143259305598No Hit
AGCAATGGGGAAATTTGTCATCCTTGAATTCATCCAGATCGATCTTGAAACA380.11886143259305598No Hit
TGCAATGGGGACTTTTGACAAAGGACTGCGGGCCTCGCTCAAGTCCCTGAGC370.11573350015639662No Hit
ACTTTAGGGATTGAGCGGGAAACATGGCGGGAAAGTGGCGTTTCATTAATTG370.11573350015639662No Hit
CATAATGGGTCTAATCCATATAATGAACAAATATGTTAAAGTGAGACAGTCA370.11573350015639662No Hit
GTTGCGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC370.11573350015639662No Hit
TGAAAAGGGGTCAATAAAGTGGCTTGTACCTTCAAATAATTTTTTGAATTTT360.11260556771973726No Hit
AGACCAGGGTGCAGCAGAAACACCAGCGTTGCTGCGACCGCGTTGGCGATGG360.11260556771973726No Hit
ATTTCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA350.10947763528307788No Hit
TTGTTGGGGAGAGTCAATAATAGAAAGGTATTTAGAAAACCCACAAACAGCT350.10947763528307788No Hit
CTCGGTTGGGCAGCAAGGAATGACATTCAGTTCTGTAAGAAATGAGTGGTAT350.10947763528307788No Hit
AAACCTGGGTGCTCTGACTCCCACTTCCACAAGCAAATGTAAAGTCTTTGTC340.10634970284641852No Hit
TGATTGGGGGGGCCAGGTGTGGGGGCATGCATTTGTAGTCCCAGTGAGATCC340.10634970284641852No Hit
CTAGCCGGGGAAATCTCTAAAGATTGAAGGAAAACAATCACTAAAATCGATT340.10634970284641852No Hit
TAAGGTGGGGCTGGAAATCCATTGTGGTTACAAGAATGTATGAAACCAAGTT340.10634970284641852No Hit
TTCTGTGGGACCGTAGCAAAGGTGATGGAAATGTACCTAGACAGTTGTAGAA340.10634970284641852No Hit
GTCATCGGGTATTGAATGACTAATTCAGTCCCTAAAGTTCTGTGAAAACACA340.10634970284641852No Hit
ACAGCTGGGTAGCATAAATCTCTTGAGCGCCAAGACAAGTATATTTAACTGG340.10634970284641852No Hit
AACGTCGGGAACTACAATATTGTTTATCTTAGTTTCATAGTAACCACAAAGA340.10634970284641852No Hit
GCACTTGGGCAGGACCTTCAGCCTTGAACGCCCATTAGTGCTATGTTATAGA340.10634970284641852No Hit
GGCTTAGGGTGGATTGCACCTCTGTAAACTATTCCTAAAATGTGTATGATAT330.10322177040975915No Hit
ACTTCAGGGGTAGGAAGCTTTCTGATGAATCTAAGTAGGTGGTATTTTGTTA330.10322177040975915No Hit
CTTAATGGGTCACTTCATGATGAATGTCATGAAAGTTTTTATTTTCATGAAG330.10322177040975915No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGAACTCGTATGC320.10009383797309977No Hit
TTGCGAGGGCAAAAGTAAAGACAACTCAAGGCTTTTGAATCACTTAATAACA320.10009383797309977No Hit
GACTCGGGCGATCTTAGGAAGGGTATGTTGAACCTTTAATGCCTAATACTTC320.10009383797309977No Hit
CTTAGGACGGGATGATGCAGTATATAAGTGGTGAACAATAACTGACAGTATT320.10009383797309977No Hit
ACGTATGGGTTCAATTTAAATCCTGGCTCCACCACTTACTAGCTGTGCAAAC320.10009383797309977No Hit
TGCACGGGACAGACACAGAACCAGATTAAGTATAATTCAGGATCCTATTGTG320.10009383797309977No Hit
TCTGGCGGGTTTTGGTCTGTAAAATGTGCTTATTTTTATCATCCTCAATTTT320.10009383797309977No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)