FastQCFastQC Report
Sat 31 Dec 2022
SRR5845257.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845257.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52420
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGCCGTCT3090.5894696680656238Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTGATCTCGTATGC2430.4635635253719954No Hit
TACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGG1170.22319725295688667No Hit
CTGAACGGGAAATGAGAATGGCTGACCTCTCGGAGCTCCTGAAGGAAGGGAC880.16787485692483786No Hit
GCAAGCGGGCAATAGAAACATTGGCAGCATGGTCTTCCAAAACAAAAAGCAG780.14879816863792447No Hit
GAGCATCGGGAATGTCTTTCTGGGTAGCTTTAGGGGCATTTTGGTGGATTAG770.14689049980923313No Hit
GTTGTCGGGGAGGCAGGAGTCAGCACTTGGACCTAGGGATGTGAGGTTTTCT760.1449828309805418No Hit
TATTAGGGAGCCAAACTAAGCTTCATAAGTGAAGGAGAAATAAAATCCTTTA740.14116749332315912No Hit
CTATCTGGGGCTTCCACGGCAGCATCTTCAGCTCTTGGGTTTAGCAAAAGTT700.13353681800839376No Hit
GATGTTTTTGTGGGGGGTTTCTGCTTTCCCTTGTTTCTTTTTCTTTCTTGCC690.13162914917970242No Hit
AGTTATGGGAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTC680.12972148035101105No Hit
GCCATTGGGAGACACACTTTTTTTGCCTTGACCTCAGTTGGTTTGTTTTGCC670.12781381152231971No Hit
TGATTGGGGCTGACTCGGGGATTGTTACTCATATAATGGGATGATGGGACAA670.12781381152231971No Hit
TTAGGCGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC670.12781381152231971No Hit
CTTGATGGGCACTGTGGCTGCCATGGTGGATGGAGGCTGGACAGAACCTGTG660.12590614269362838No Hit
CTACCGGGCAGTGTGTCACCATTTTAATTGGTGTCTATGACATACAGACAGG660.12590614269362838No Hit
TCAGCTTTTGGCCTCCTTGAGTAAATGACTTCTCCACTGCTGAATAGCCTAC660.12590614269362838No Hit
CTAATTGGGTGCATGTCAAATACTTTGTGATAGTTAAATAAGACTACAGGAC660.12590614269362838No Hit
TAACCGGGGGATCACCTTGAAGAAGTATGACGGTCGAACCAAACCCATTCCA660.12590614269362838No Hit
GTGAATGGGCCATCGAACGAAGTAACCAAAACAGGAACTGAAAATGAAAATA660.12590614269362838No Hit
GCTTGGGAGGCTGAGGCGGGAGGATTGCTTGGGCCCGGGAGGTGGAGGTTGC650.12399847386493704No Hit
AGCTCTGGGGACCAGCTGGGCATGCGCATGCAGCACAACCTGGAGCAGCAGA650.12399847386493704No Hit
TCGCACGGGGGTGACTCGACGTGGTGAATGTGCACACGCCCTGCCTGGTCCG650.12399847386493704No Hit
CGTTGTGGGGATCCTAAGGGAATTTTAGTTGACATTAGATCAGTTAATATTA640.1220908050362457No Hit
AACTCTGGGCTGAACGAATGAGCACACTTTAACCAGTTATTTACAGTTACCT640.1220908050362457No Hit
TTGACTGGGTCTATAGACGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCT640.1220908050362457No Hit
ACACAAGGGAACGACATAAATTGAAAAACACTAAATTAGAAAAATCAGGCAT630.12018313620755436No Hit
CGTCAAGGGCGATCTAGTTCCGGGAGGAACTTAACACTTGTTTATGTCGATT630.12018313620755436No Hit
TTTTCGGGGGAGAGGAAGGGATTTGGAAACCATGTACTTTTGGGGAGTAATT630.12018313620755436No Hit
TGCAAATGGGATAAAATTCACTTGATGGATTTTGTAGTGATGTTTTCACAGT630.12018313620755436No Hit
GACTGCCGGGGGATGAAGCCCGGGGCAAGAACTGTGGTGTCTTGGTACATTG620.11827546737886302No Hit
CATTTTGGGATCCAGTCAGCTGCATGGTACTGACTGAGCTGAGGAGTCAAAC620.11827546737886302No Hit
GACTTAGGGGGGATTTTTTAGGAATCTATTATGCTGTGATGCGGCTGGCACC620.11827546737886302No Hit
AAAATCGGGGCTGTCTCTGCAAATTTAAATCCATCTTATTTCAGAATGCTGG610.11636779855017168No Hit
TGTTATGGGCGATCTGCCATGGGGAGTGCCCCAGAAAACATATTCTGTCAAA610.11636779855017168No Hit
TGGTCTGGGATTGGTGAAGTAGTATTTTAATTCACCCACCAACATTCTATTG610.11636779855017168No Hit
CACAATGGGGGGTAAACGGGCTGTGTGAGAAAACGGCCCTGACTGTAAACTG600.11446012972148034No Hit
CGCTTTGGGCACAGGAGACACAGGACTCCATGTCTATTGGTAGGTAGGTTAT590.112552460892789No Hit
ACTCATGGGAGATATATGGTATCCATTTAGATGAAGGCTAGGAAGGAATCTT590.112552460892789No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAAGTG590.112552460892789No Hit
GATGTGGGGGCTGGGGATGAGAACTGAATAATCTTTGCCTGGAGTGACACTA590.112552460892789No Hit
TGCTATGGGGAAGATTTGCCATATTACTATTTTGGAAACATTGAGTGATAGA590.112552460892789No Hit
GTTAAAGGGGTAAATAGATGATTAAGATTACATTAATACAAAAAATATATTG580.11064479206409766No Hit
CAAAATGGGGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCT580.11064479206409766No Hit
GGCTAAGGGACAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCCTCC580.11064479206409766No Hit
CTGTGTGTGGGATCTTTGTGTTTAGCTCATCCAAAGGTGTTACCCTGGTTTC570.10873712323540632No Hit
GAGTTAGGGACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCAAC570.10873712323540632No Hit
AATGTAGGGCGGAGTTCTGCTCCATATCCACCAGAAAGCTGGCGGCCCACGG570.10873712323540632No Hit
ATGAAGGGGCAGTGGTGCCATCTCAGCTCACTGCAACCTCCACCTCCCAGGT560.10682945440671499No Hit
TTATATTGGGCATATTACCACTGGCAGTCACTGCTACAATGTGGGCTGTTCT560.10682945440671499No Hit
ACTCAAGGGTCTGTAGGTGGGCTGATCCACATGCGTGTGGCAGCCCAAGGCT560.10682945440671499No Hit
GTTCTAGGGATAAAAGTCATGCAGATTAAAACTGTGCTAAAATATTTTTTCT560.10682945440671499No Hit
ATTACAGGGTTAATCACTATGTAAGTGGTGAGAGATGCAGGACATACACATT560.10682945440671499No Hit
GGTGGTCGGGTTAGAACCATTTTTTTACCCTAAAACATCCATTGTAGTTTGT560.10682945440671499No Hit
CGCTTCGGGATTCTGAGGGGCCACAGTAATTACAAACTTACTATCCGCCATC550.10492178557802365No Hit
GCCATTGGGACATTTAGAACCTGCATTTAAGAACAGAACAGCAAGTATGAAC550.10492178557802365No Hit
CCGCTAGGGATCAAATAATAGGTCAAGCAGGAAGGAGCTGAGTTCTAACCAC550.10492178557802365No Hit
CTAGAAGGGAGTCAAGAAAAGTGACCCACCTTGTTACTTGACCAGCAGTCTA550.10492178557802365No Hit
GCTTGCGGGGCTAGGCCCTATTCTAAGTGTTGCAACTATTAAATAGAGTAGT550.10492178557802365No Hit
AATAGGGGAGATAAGGGACATTCAACCTGTAATGTCAACTCAAATGTGCCAA550.10492178557802365No Hit
CTATCCGGGGAGAGATTCAGGGATAATCCACTGGACTTGGTGATAGACTAAA540.10301411674933231No Hit
AAAAGTGGGTAAGCCAATAGGGTTACAGGTTGGTACAGTGACACATTGAGAA540.10301411674933231No Hit
CACGATGGGTCATGTTTCTACACTTGCTTGCCTCGGATCTCCCTCCACTGCC540.10301411674933231No Hit
CTTATGGGGGAGAAAACAATGGACAAAAGTACTGAAAAGACAAAAAAGGATA540.10301411674933231No Hit
TCTGAAGGGTCAGCACTGAATATAATATCCTTTGTTCATGAATTGGAAGAAT540.10301411674933231No Hit
TATCTTGGGTGAGAAGATGAAAGCATCTTTAGAAGGGCCACTTAAATTCACT540.10301411674933231No Hit
CTGCTACGGGGGGATGATCCTGATGCAGCCAATTATAATGATATCAATGATG540.10301411674933231No Hit
GTATACGGGAACCCCAGCCCAGTACCTTTCTACGTTTCTTCCCACTTTCCTC540.10301411674933231No Hit
AGAACGGGGGATTTTAAACTTTACTATATTTGTATTCCTGTCTCCCCACCCC540.10301411674933231No Hit
ATTGCTGGGCATCTATTGGACCCTCTTTACACTGGGCCTGGATCTTTCTTGG530.10110644792064097No Hit
TGGCTAGGGAAGGGAAGAGACTAAGGAGATTGTATTCAATATGTATGAAGTT530.10110644792064097No Hit
CTTATACCGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAG530.10110644792064097No Hit
TTGAAAGGGGTGACATCAATTAGGAGGTTGTCCTACTAGCCTATGACAAAAG530.10110644792064097No Hit
ACAGGGGGTATAGCACAGAGGCTCTGGTGACTAGGCTGTTATCAGGGAGAGG530.10110644792064097No Hit
ACTTGTGGGAGACCCCCAAGACCATCATTCTTGCCCAGGCTTCAGAGACAAC530.10110644792064097No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)