FastQCFastQC Report
Sat 31 Dec 2022
SRR5845246.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845246.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123140
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCAAGCGATCTCGTATGC6260.5083644632126035No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCAAGCGATCTCGTATGCCGTCT5450.44258567484164363RNA PCR Primer, Index 42 (95% over 21bp)
GCTTCCGGGAAACCTTTAAAACGATGGTAAGAAGAAAGTACTCTCATTGCAA1940.15754425856748416No Hit
TTATGAGGGACTTCTGTTGTGGTTAAGTACACAGACAACATCATGAAAGGCT1640.13318174435601754No Hit
CTCCACGGGTATAAGATTTATCTAGTGTATCTTTTGGGTTTTTGTAGTAAAG1620.13155757674191976No Hit
GGTAATGGGGGAGGAGAAGGAACTTCTCATACAGCTGTTATTATTGATGAAA1590.1291213253207731No Hit
CGTGGCGGGCGCCCGCTTTGGCCTCCCGGGGTGCTGGGATTACAGGCGTGAG1590.1291213253207731No Hit
GCTTATGGGCCGATCTAACCAGGGGAACCTTGTCCTTGTAATTACATTCATT1530.12424882247847976No Hit
TGCATAGGGAATGAGCTAAAGACTCTAAAAGAGTAGCTGGTGCATATTAAAA1530.12424882247847976No Hit
TGCAGTGGGGGATGCAGAAGTTAATATTCCACTTAATGTTATCTGAGCATTA1520.12343673867143089No Hit
TGAGGTGGGGTAGAACTAAGATCTTCTCTTCATTTCTCCCTTACAGTTGGCT1500.1218125710573331No Hit
ATTAATGGGCATCCTAAAAGTTAAAAACATGAAAAGCCGCCATCTTGTCATC1480.12018840344323535No Hit
CTTCAGGGATACACGCTTTGTCTGCAGGGAAGTTCTCCTGGTAATTACAAGG1470.11937631963618645No Hit
TACGTAGGGGTGGCTTTACATTTCAAAAGTGAACTTGATTCAACCTGAGAAA1440.1169400682150398No Hit
CATGTCGGGAAGATATAGTCATTCTTCTTCTGCAGCACAATATTGATGTGTT1430.11612798440799091No Hit
CAATGACGGGAGTATTTATAAGCTTTCTTTGACAACAATTCATAAGTTAAAA1430.11612798440799091No Hit
GAGGCTGGGGAATCCGGAAGTTAAGAGGAAATGTGTCATGTATGAATCTGGC1410.11450381679389314No Hit
CAGACAGGGGGTGGTTGGCCAAGTAAATTCACTAACTAGATGACTTGCTCCT1400.11369173298684424No Hit
GATATGGGGGTGGAAAGCGCAAGGATAGAAGTAGAGAGGCTGATTAGGAGCA1400.11369173298684424No Hit
CTCGTAGGGCATCAGAAATGGGAGCCTCAGTCTTGGTGACTGAGTCCTCAAA1400.11369173298684424No Hit
GTTACTGGGACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTAT1390.11287964917979536No Hit
CTTCAGGGGTAAAAATGGGAAACTGGAGAGAGAAAAAAAATTGTTTCAAGAA1390.11287964917979536No Hit
TTCTCAGGGGGAGATTAAGTGTCTAAACTTAACTTAAATGGTTTTTTACAGG1380.11206756537274648No Hit
CGCACTGGGATGTTTGCAGGGTTTAAAAATCAGTAAAAATGGGAATGATTGA1380.11206756537274648No Hit
GTAGAAGGGGATATCATTCAAATAAATGATAAGTAGTAAGGATTTGGTTCTT1360.1104433977586487No Hit
ATTACAGGGACCACCTTTCATTTTCCCTCTGTTACCAAATTTCAGCTCTTAG1360.1104433977586487No Hit
TCGGTTGGGATTCTCAACATGCTAAACATTAACTACAATTCACTGTTGTGAG1360.1104433977586487No Hit
ATCTTTGGGAGGCAGTCCAAAAATCTACATGTGTCTCAATGCATACAATGAA1360.1104433977586487No Hit
TTCAGTGGGATCACATACATGCACAAACATGCACACCTCAAGATACATGCTT1350.1096313139515998No Hit
GAAATTGGGCAGTATTTTAAAATTTTCAACAGTATTATAAAAACATTGCATC1350.1096313139515998No Hit
TTTGGAGGGATATTATCACATCTACACAGTATCTTCATAGCAACATCTAGAT1350.1096313139515998No Hit
TAACTAGGGATTGAGTTATCAAAATATTAAAATTTGTGATTGGCAATGCGTG1340.10881923014455092No Hit
CGTTCTGGGGGAGTAGGTACTAATGGGAATGGCATTTCTTTTGGGAGTGATG1340.10881923014455092No Hit
GCCGGGCAGGACCTTTTAATCTAATGACTCAGCTCTTCTGTGTCTCTGTTTT1330.10800714633750204No Hit
AAGGCTGGGAGGAAAAGGGCTAAAAACAAATGGATGAAAAAAGGTATACCAT1330.10800714633750204No Hit
CCTACGGGGGGTAGAGGGCGGTCGCGGCGGGCAGTGGCGGCAGAATGTTGGC1320.10719506253045313No Hit
TAGCTAGGGTCTGTCTGACATTTGATAATCTCTCAATTAACATTAGTGGTTA1310.10638297872340426No Hit
AATAAGGGACTGTTTAAAAAATTGTTTTTCTTTTATTTAGGTCTTTTTACCT1310.10638297872340426No Hit
ATAGCGGGATGTCCATTCTGAGGGAACACTAGATATAAATGGCGTAACTCCC1310.10638297872340426No Hit
TCTAAGGTGGGTAACTGCTGAGAGCAGCCTGTGATTGTAGATTTTTATGGGT1300.10557089491635538No Hit
GCTGCGGGTCATTCAGGAAATGGATCTCTTTATACATCAAAACCTCTTGACC1300.10557089491635538No Hit
TACATAGGGCTTCAGAATTGGAATGTTTATATTATGTAGAAATCTTCAAAGG1300.10557089491635538No Hit
GCTGATTTCCCCTTACCAGCAGGTGGAGTGAGAATCTTCAGAGCTCTGGAGT1290.10475881110930647No Hit
AACTGGGGAGACAAAATTAATCTAGGATGGAAAATATCACAGTGGTGGTTGC1290.10475881110930647No Hit
TAAGACTCGGGAAGGCAAACTGACAGTGTGGTATTAGAAATAAACATCAAAC1280.1039467273022576No Hit
CAGACTGGGCCTAAAGAGGCTCCCAAGTCCATAAAAATACTGGTGATGTCAA1280.1039467273022576No Hit
CAATGTGGGAGGTTGCAGTGAGCCAGGATGGCACCACTGCACTCCAACCTGG1280.1039467273022576No Hit
CTGATAGGGGTCCAGCCTGGCTAAATGTTTTTAGACTTAAGAGATTAAAACA1280.1039467273022576No Hit
AACAGCGGGTAACACTTACATTTTATCCTGTAGATGTTGAAGAAAACACAGT1280.1039467273022576No Hit
ACAGGGACGTTGACAGTGTTGAAGCAGGGTGAATAACTAGGGCATATATATT1280.1039467273022576No Hit
CGGACCGGGATCAGTTATTAACCCACAATTATAACTAAGCGTGAGTTATGGC1270.10313464349520869No Hit
CATTAGGGGAAGGGAATTGCTTTCCAGATGCTAAGAAGCAGCAGTGGGGCTT1270.10313464349520869No Hit
AAAAGAGGGGGCGATGATGCCAAGAAAGTGAAATGGGTGGACATTAATGATA1270.10313464349520869No Hit
GCACGCTGGGAACCAAGACCAACCTTAGAAAAGCCTTTGATGCCTACCTTGA1270.10313464349520869No Hit
TTATTTGGGATTTAACATTACTATTCTAAGGAAGGACAGTCATGTCAACATT1270.10313464349520869No Hit
CAGAGGACTTTTGGAGGAGAGCAGTTTCGCAACTTTGTTCCCAAAATACAGG1270.10313464349520869No Hit
TATGCTGGGTGAGCTGAATTTTTCCTTTTTGTATTTTCTTAGCAGAGCTCCT1260.10232255968815981No Hit
AGCAGAGGGAGTACTTTCAGACTTATTAATAACTGTTGGAAGAAATACAAAT1260.10232255968815981No Hit
ATGCTGGGGTGGGAGAATTGCTTGGACCCGGGAGGCGAGGTTGCGGTGAGCT1250.10151047588111094No Hit
AATAGTGGGTTCTAGTGACCCTTTAGCGGCAGGTATTTATACCTGGTATTTA1240.10069839207406203No Hit
GGATTGGGAATATACAAAAATCCATTGTATTATACACTTTTCAAATGGGTGA1240.10069839207406203No Hit
ATTTAGGGGAGATATCTGGTTGGCAGCTTGGAATTGGGTATCCCTGATAGTG1240.10069839207406203No Hit
GCTATAGGGGTGGTATGAAAATTCACCAACAGGATTAAACATTGTACTATCC1240.10069839207406203No Hit
AATTTAGGGGTGTGAAGAAACACTAGCTTATAAATTCTTAGTGAGATGAAAC1240.10069839207406203No Hit
AGCAACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1240.10069839207406203No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)