FastQCFastQC Report
Sat 31 Dec 2022
SRR5845245.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845245.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122965
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCAAGCGATCTCGTATGC6020.48957020290326514No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCAAGCGATCTCGTATGCCGTCT5140.4180051234091002RNA PCR Primer, Index 42 (95% over 21bp)
GGTAATGGGGGAGGAGAAGGAACTTCTCATACAGCTGTTATTATTGATGAAA1790.14556987760744927No Hit
CTTCAGGGGTAAAAATGGGAAACTGGAGAGAGAAAAAAAATTGTTTCAAGAA1730.14069044036921075No Hit
GCTTCCGGGAAACCTTTAAAACGATGGTAAGAAGAAAGTACTCTCATTGCAA1720.13987720082950433No Hit
CTGATAGGGGTCCAGCCTGGCTAAATGTTTTTAGACTTAAGAGATTAAAACA1710.1390639612897979No Hit
CTTCAGGGATACACGCTTTGTCTGCAGGGAAGTTCTCCTGGTAATTACAAGG1610.13093156589273372No Hit
CGTGGCGGGCGCCCGCTTTGGCCTCCCGGGGTGCTGGGATTACAGGCGTGAG1600.13011832635302728No Hit
TGCATAGGGAATGAGCTAAAGACTCTAAAAGAGTAGCTGGTGCATATTAAAA1600.13011832635302728No Hit
TTATGAGGGACTTCTGTTGTGGTTAAGTACACAGACAACATCATGAAAGGCT1580.12849184727361443No Hit
CAGACTGGGCCTAAAGAGGCTCCCAAGTCCATAAAAATACTGGTGATGTCAA1580.12849184727361443No Hit
GATATGGGGGTGGAAAGCGCAAGGATAGAAGTAGAGAGGCTGATTAGGAGCA1580.12849184727361443No Hit
GGTAATGGGGTAATGAAATGGAGGTACATGCATCAAGCAAGCACTTGACAAG1580.12849184727361443No Hit
GCTTATGGGCCGATCTAACCAGGGGAACCTTGTCCTTGTAATTACATTCATT1530.12442564957508234No Hit
TGCAGTGGGGGATGCAGAAGTTAATATTCCACTTAATGTTATCTGAGCATTA1510.12279917049566949No Hit
TTTGGAGGGATATTATCACATCTACACAGTATCTTCATAGCAACATCTAGAT1500.12198593095596308No Hit
TTACTATGGGAACCACTAAGTTAGATCTCTACCTCACAGTGTCCATACAAAT1490.12117269141625667No Hit
TTCTCAGGGGGAGATTAAGTGTCTAAACTTAACTTAAATGGTTTTTTACAGG1480.12035945187655024No Hit
CCTACGGGGGGTAGAGGGCGGTCGCGGCGGGCAGTGGCGGCAGAATGTTGGC1470.1195462123368438No Hit
ATGTATGGGAGGAGCGTGCCGTTTCCCCTGTAGATGGATACTTTTGTTTTGT1440.11710649371772457No Hit
AACTGGGGAGACAAAATTAATCTAGGATGGAAAATATCACAGTGGTGGTTGC1440.11710649371772457No Hit
TGGACTGGGAGATTTTGACAATTATTTGTCAGTGTTCTAATTTCTTTTAGGG1440.11710649371772457No Hit
ACTCGGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTAC1430.11629325417801813No Hit
CATTAGGGGAAGGGAATTGCTTTCCAGATGCTAAGAAGCAGCAGTGGGGCTT1430.11629325417801813No Hit
GCATACGGGACACCACTGCACTCCAGCTTGGGTGACAGAGTGAGACTCCATT1430.11629325417801813No Hit
ACAGGGACGTTGACAGTGTTGAAGCAGGGTGAATAACTAGGGCATATATATT1420.11548001463831171No Hit
CAGACAGGGGGTGGTTGGCCAAGTAAATTCACTAACTAGATGACTTGCTCCT1400.11385353555889888No Hit
GCTGATTTCCCCTTACCAGCAGGTGGAGTGAGAATCTTCAGAGCTCTGGAGT1400.11385353555889888No Hit
GCCGGGCAGGACCTTTTAATCTAATGACTCAGCTCTTCTGTGTCTCTGTTTT1400.11385353555889888No Hit
TGAGGTGGGGTAGAACTAAGATCTTCTCTTCATTTCTCCCTTACAGTTGGCT1400.11385353555889888No Hit
ACAGTTGGGGCCCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAA1400.11385353555889888No Hit
TTGAGAGGGCTAGTTTTATCACATTAAGAGGCTCTCCATGCACAAAATGCAT1390.11304029601919247No Hit
TAGGAAAGAGAGTATGCGTGTCTGTGCACTACACATTCACAGAAAATCCTCT1390.11304029601919247No Hit
GCTGCGGGTCATTCAGGAAATGGATCTCTTTATACATCAAAACCTCTTGACC1390.11304029601919247No Hit
TACGTAGGGGTGGCTTTACATTTCAAAAGTGAACTTGATTCAACCTGAGAAA1390.11304029601919247No Hit
TGTACAGGGGAAAAACACAAATGAAAATAATGATGAGATATCATTTCACACC1380.11222705647948603No Hit
CAATGACGGGAGTATTTATAAGCTTTCTTTGACAACAATTCATAAGTTAAAA1380.11222705647948603No Hit
AGCAACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1370.11141381693977961No Hit
GAGTTTGAGATTCAAACCCAATTGACTATAAATCCCAGACTCAGAACCACTA1360.1106005774000732No Hit
ACATATGGGGATAACAGTAAAGGTGGCGGGAAATCCTCGGATTCAGGATGTA1360.1106005774000732No Hit
CGCTATGGGGCAGGCGGGAGTCTTCAGGATTTGTTAATAAAAGATAATCTTT1340.10897409832066035No Hit
GAGGCTGGGGAATCCGGAAGTTAAGAGGAAATGTGTCATGTATGAATCTGGC1330.10816085878095393No Hit
ACTTAAGGGAGGAAGAGGCTTGTGCCGTTATTCTGCTGGAACATGGTGCCAA1320.10734761924124751No Hit
TGCTATGGGGGGCAGCTGTGGGTAAAATATAACTTTACTTATGTGTACACAT1320.10734761924124751No Hit
AAGTAGGGGCTCATAGGTCCAGGATTTGAACCTCAGGAGCCCCATGTTCTTT1310.1065343797015411No Hit
CGGACCGGGATCAGTTATTAACCCACAATTATAACTAAGCGTGAGTTATGGC1300.10572114016183466No Hit
ATATTAGGGCTAAGAAGAAGGCCTTTACCAAGTACTGCAAGAAATGGCAGGA1300.10572114016183466No Hit
TAAGTCGGGGGAGGCTGCAGGGGCTGCTGGGGCCTCAGCTGCCAGCTACTCG1300.10572114016183466No Hit
AGCAGAGGGAGTACTTTCAGACTTATTAATAACTGTTGGAAGAAATACAAAT1300.10572114016183466No Hit
GATATAGGGAAAGAGAAAGTTATATAGATTCCTTGCTGTTCCTCATCTGTGC1290.10490790062212825No Hit
GGTTTCGGGTATGGAATTTGTTACGGGCCCTGGTCCATAATGGTGGCAGCCA1290.10490790062212825No Hit
ATTAATGGGCATCCTAAAAGTTAAAAACATGAAAAGCCGCCATCTTGTCATC1290.10490790062212825No Hit
GGTAATGGAGAGTTCAAAATGGAGAATCTTGACCGGCTACAATATATTTGAA1290.10490790062212825No Hit
CGAATGGGATGCTAACAGGATCTACAGGATGATCAAACTTGGTCTGGGTATT1290.10490790062212825No Hit
TTAGCAGGGAACTTATAAAAGTTAAAGAAAAGAGAGATGCGACCATTTCCTG1280.10409466108242182No Hit
GTCTATGGGAGAGTAAATCCAAGTCTGCAGTCAGGACACCCCAATCCTCAAA1280.10409466108242182No Hit
TGCTTAGGGATTACTAGGTTATGTGGTGGTTTAATTTAATATTTTGAGGAAT1280.10409466108242182No Hit
CGTGTGGGGAGACAAGGTTCACAATATTCTTGCAGAAATGGTGATGGGGGGA1280.10409466108242182No Hit
ATTTGTGGGTAGAGGCTATACCTCAAAGTAGCTGCGGATTTTGTCTCCTGCA1280.10409466108242182No Hit
CTATACGGGGATCGGACCAAGTTACAGTGTTTGAAACAAGTCAGCAGAATTC1280.10409466108242182No Hit
CAACCAGGGGACCATATCTGCAAATTGTAAGTACATACATTTTGATGACCTT1280.10409466108242182No Hit
TGCCTGGGGGTTAAAATGTTGTATCTTGTAAACAAAAGAAAGATTGCCTGCT1270.10328142154271541No Hit
ATTACAGGGACCACCTTTCATTTTCCCTCTGTTACCAAATTTCAGCTCTTAG1270.10328142154271541No Hit
GTTACTGGGACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTAT1260.10246818200300897No Hit
TAACTAGGGATTGAGTTATCAAAATATTAAAATTTGTGATTGGCAATGCGTG1260.10246818200300897No Hit
GATTTAGGGAAGCAACTGGATTTTTTAACTGAAGATAGTGCCTTTCAGCCCT1260.10246818200300897No Hit
ATGCAAGGGGAGAGAGAAGTTAAAGACATGAGCCTTATTGTGGCTTGTTTGC1260.10246818200300897No Hit
ATTTAGGGGAGATATCTGGTTGGCAGCTTGGAATTGGGTATCCCTGATAGTG1260.10246818200300897No Hit
GAAATTGGGCAGTATTTTAAAATTTTCAACAGTATTATAAAAACATTGCATC1260.10246818200300897No Hit
CCATATGGGAATCAAAGGGCTGGGGAGACAAGACTGTGGGCACAAAATTGCC1260.10246818200300897No Hit
CGTATAGGGACTCACCTTTCATGGTCAAGAGCTGCTCAGTGACACCAAACTG1260.10246818200300897No Hit
CGTCTGGGGCAACATAGTCGCTCTTAGATTCAGGTTCAGACATCTCCAAATG1250.10165494246330256No Hit
TTATCGGGGATGAATGCTAAGGCAGAGCTTTAGAAATGTTCTTGTTCTTTGC1250.10165494246330256No Hit
ATTCCCTGTACCTCAATTTCCATGTTGACTATATGGTGATAATTCTACCAAA1250.10165494246330256No Hit
TGACGAGGGTGAGACTTGCCATAAGAAACTGCTGAGTACTTGGCAAACCCTT1250.10165494246330256No Hit
TCTTGAGGGAACATACTTCTGGGAATAATTTGGTTTCACCAGATACAGACTA1250.10165494246330256No Hit
GGAGCTGGGTCTATGTGAGGGAGTTGCGATTCTTGCAGCATCAAATGGCTAT1250.10165494246330256No Hit
TTGTGAGGGATTGGAGAAGAGATAATACCAGGAATTCGTTTGAAGAATACTT1250.10165494246330256No Hit
TAAACCGGGGGAGCAGATCGCCACATTGAGGGCGGTGTTGAAAGCCAACAAG1240.10084170292359615No Hit
ACTGCAGGGAGGCTGTTAAGATTAAAATTAACATTTTGGGGACAGAATTGTG1240.10084170292359615No Hit
CATGTCGGGAAGATATAGTCATTCTTCTTCTGCAGCACAATATTGATGTGTT1240.10084170292359615No Hit
TGATGAGGGTGGTGTCTGCATATTGAGGACATAGCCATTTCCTTTAAGGAGC1240.10084170292359615No Hit
TCGCATGGGGTGACTGAAATGGCTTTCATCATCTTTAAGTCAAAGTGAGATG1240.10084170292359615No Hit
GCGCAAGGGACGCGTGGGGAAGGAAAAGGTAATATTGGAGTTCTTCTTCAAT1240.10084170292359615No Hit
AGCTCAGGGATTATTCATGGGTAAAAGATTCAACAAGAGTACAAATGAACAA1240.10084170292359615No Hit
TCGCAGGGATGATAATAACGAGCAAAACTGGTTAGATTTTGCATGAAATGGT1240.10084170292359615No Hit
AATCAAGGGGGCACAGCAGAAGTTCAGTAATTGAATTTTATCAAAAATGGTC1230.10002846338388974No Hit
GCACGCTGGGAACCAAGACCAACCTTAGAAAAGCCTTTGATGCCTACCTTGA1230.10002846338388974No Hit
TTGGTGGGGAGACTAAATGTTAAGAGGAAAGGGGAGGAGTCTAGAATGATTT1230.10002846338388974No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)