FastQCFastQC Report
Sat 31 Dec 2022
SRR5845220.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845220.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26093
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCAAGCGATCTCGTATGC2871.0999118537538803No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCAAGCGATCTCGTATGCCGTCT2440.9351166979649714RNA PCR Primer, Index 42 (95% over 21bp)
TTACGCGGGAGTATCTCTCCTTTCAGACTGTTTTCACATGTACAGGCGCATA2370.9082895795807304No Hit
ATGCTGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC2310.8852949066799525No Hit
CAATGTGGGGACGCTCTTCCGATCTCTCTATGGGTACTGTTTCCTTATCAAA2290.8776300157130265No Hit
AGTAGTGGGAACTTTTAATAGTTTTGTGTATCATTCAACTTTTTTTCTTGCA2260.8661326792626375No Hit
TCGTCAGGGATACTATTAAAAGTAAAGTCAGTGCAGAAAATCACTTACGGAA2240.8584677882957115No Hit
TACGCAGGGGTAATAAGGAGATTTTTCTTTCCAAAGCAATTCACAAGTCCTT2130.8163108879776184No Hit
CACATCAGGGAACCAGGTGGTAGATATAATCAAATTGTTTTGCAGAATCAAT2120.8124784424941556No Hit
TGTAAAGGGTATCCAGGATGACTGTGTTGCTGCAGTCTTTGAGTGGTTGGAG2120.8124784424941556No Hit
TACGAAGGGTCACTGATGTAGTACCATATCCAATATCTCTGAGATGGAATTC2030.7779864331429885No Hit
CATTGAGGGATGAAGGATGAGGAACTGAGGCGCATGCAGGAGATGCTGCAGA2020.7741539876595255No Hit
GGTAGGGGGAGAAAAAAGTACATGTGAAATACTACTACTTATCCATCTCCTG2020.7741539876595255No Hit
ACGGTAGGGGGCTGTGAAAACAGTTTTTCTTTTGCTTGTTTCTTCCTGAGTT1970.7549917602422106No Hit
CTACGTGGGCTACTTAAAAATAAAGACCAACAAAAAATATAGTTGTTTTTAA1960.7511593147587475No Hit
TTTATTGGGGGTGGAGAATATCAAGGATCCTTTGTTCAGGTTCTTTGAGAGA1960.7511593147587475No Hit
ATTTTGGGGCCAGGCTGAGTCCATACTGCCCCTGATCCCAGAAGGAATGCTG1830.7013375234737286No Hit
CATCCAGGGAAACCCTGTATGTGTTTATATGTGCTTCACAGAGTTCGTGTCA1780.6821752960564136No Hit
AGTCCTGGGAGATGTGAAACAATTGAAATTTTCATACATTGCTGATAGAAAT1770.6783428505729506No Hit
TACTAAGGGTCATCATATCTGAGTTCATGCTAATGAGGTAACTATTGTTGGG1770.6783428505729506No Hit
AGATAAGGGATCCATTTTTTATTATTTTCTTAGTTTTGTTCTTGGACAAATT1660.6361859502548577No Hit
TCTATGGGGAGTAGGCTGGGCGGGGAGCGTCGGACCAGAAGAGAATCACGCC1640.6285210592879317No Hit
AAACAAGGGGGGAGGGAGCGCGCCAGGCCTGGCGCGCACCCGACCCCTTGCG1580.6055263863871537No Hit
GTAGGGGCTGGATCTCCGTATTTAAAGTTGTAATTCCACTTTACTGTTTTCC1540.5901966044533017No Hit
TTTGTTGGGGATACATTTATTTAAAATTCCTTGTGGACTCTGGATATTAGAC1540.5901966044533017No Hit
GGAATAGGGGATCTAATATCGGGGAAAATGGGCTGAAATAGAAGAAATTGAT1530.5863641589698386No Hit
GCCCAGGGTGGTGCCATCCGAGAATTTGCAGAGCACATTTGCCTACTAATGG1530.5863641589698386No Hit
GCTTCTGGGAAGTTTTAAATCACAATCTGGAGAATTTTCTATGATTAGGAAG1510.5786992680029126No Hit
AGGCCCGGGTGTCTTACTGCTAGCTCTTCTAACAGGGGAAGTCTGTATGAAT1500.5748668225194496No Hit
GTGTAAGGGGGACCATTGTGGATTGCTCTTAGCTATTTTCATGTCAGATGCA1500.5748668225194496No Hit
GTCCTGAGGGCACGGTATTATTGTTTCCTCAGAAGAGTGTGTATTTAAATAC1500.5748668225194496No Hit
GCACATGGGATCATCATTTGCTTCAAGCCCCGAAACACAGCAGAGCTCCAGC1490.5710343770359867No Hit
GCACTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1410.5403748131682827No Hit
ATGGCCGGGACCTCAAGGAACTAGAGAAGAGCAAACTGAGCACAAATGTAGT1390.5327099222013566No Hit
ATTTCGGGGTTCAGTTCACGGCGATAAAGAAGTAGGTGCTGCACACAAATAT1370.5250450312344307No Hit
GTCCCTGGGGTGCTGGGATTACAGGTGTGAGCCACCGTGCCCAGCTAGATTC1350.5173801402675047No Hit
TAATGTGGGCAGCCCAGTCCTGCATGTTTGAAAGCATCAGCTGCTTGAAAAA1330.5097152493005787No Hit
CATAATGGGAACAGTGTCTCTCTTTCAACGCTATCATGGAAGAACTGGGAAT1310.5020503583336527No Hit
GCTAGTGGGGGTATGTTTCTGGTCGGAGGTGTCATTTGTGGTTTATGGCCAT1280.49055302188326366No Hit
CGCAACGGGTGCCACGACCCGGGGAGTTTATCATGCGGCTTGTGTGTTTCAG1240.4752232399494117No Hit
AACCCGGGGTTACAGGAAGGCGATCTTGTTGGGCTCAGCGTTACGTGTATCT1210.4637259034990227No Hit
CTGTTTGGGCATGGCTCATTGCTTACTGTGATTCTGCTCTTTTTTTCTTTCT1190.45606101253209674No Hit
GCTATAGGGGTGTAATAGCCCAGCCAGTGGTTGAAATGAAGTACCTGCCCAG1160.4445636760817077No Hit
GTTTCAGGGATGCTTGGAGCATTCTCATTCGAGAGGCACAGGTTTAGTGATA1140.43689878511478175No Hit
GCAGAGTGGGCACACAGAGCTGTTGGGAAACCTCAAAGCAGTAAATCAAGCA1130.43306633963131874No Hit
AGAGTTGGGATTGTTTGTTAAAAATGTTTTGAAATTATTAATGTGACTATGA1120.42923389414785573No Hit
AATATTGGGGGGCCCTCATTTGGCCAATAGCATCCTTTTCCAGTCTTCCGGT1120.42923389414785573No Hit
GTCGGTGGGCAGAGGACGAGGAAACAATCGTGGCTACAAAAATCAATCTCAG1110.4254014486643928No Hit
AGTTGGGGGAAGGTCCCAATTTCCTCGAATAAATAGGCAAACAAGAACTAAT1110.4254014486643928No Hit
CTACCCGGGGGGAGGCCAAGGCAGGCAGATCACCTGAGCCCAGGAGTTCGAG1100.42156900318092977No Hit
CAGTTGGGGATGTTTATCAAATCGTGTACTAAAATGGAAAGCTAGTTTTGAG1100.42156900318092977No Hit
AGAAGGGGGTTTTATAGTTTTAGAATATTAAAACCTCAGATATTTATGTGGG1090.41773655769746676No Hit
GCTTATGGGATACATTCCATTATTAAATTCTGCCCTATTCCCAATTGTTAGC1090.41773655769746676No Hit
AACTAGGGATTAATTAATCACATTATTTTCTGCCCTGAAATCCCAAAATACT1090.41773655769746676No Hit
GTTATAGGGTATATGTTGGGAAACTTCTTAAGGTTGGAGAATGAACCACTCA1080.4139041122140037No Hit
CCATCAGGGATGCTCATTTTCGGGGCTTCCTTGCCAGGGCTCCCACTTCCTG1050.4024067757636147No Hit
CATAGTGGGCGGTGCTGCTGGGCCTCCAGTAAATCTTATCCCCAGCTGCTTC1020.39090943931322575No Hit
CTCTCCGGGAACAGAAGTTTATTTTCTCATAGTTCTGGAGGCTGGACATCCA1010.38707699382976274No Hit
GTGCTGGGTTATGGCAATATTCGAGCTGTGGCTAGCAGATCAGCAGATCGAT1000.3832445483462998No Hit
CTTCTCGTGGGTGACTTTAGCTGCATTTCACATATTTTAATATGTACACTTC990.3794121028628368No Hit
AAACATCGGGCTCTGTAAATAGTTGTTATACTATATTGTTTAGGGAATAATG980.37557965737937377No Hit
TGCTTTGGGACATATAATATATACATGTACATATAAAGTATTTAAAATAAAG950.3640823209289848No Hit
AAAACAGGGATTCACATTTTATATAAACAACCTACCAAACAAAAGTACTTCC950.3640823209289848No Hit
AGCCTGGGCAACAGAGTAAGACAAAAAAATATATATATATTGAAAATCAAAG930.3564174299620588No Hit
TACTACGGGGATCTAAGCGAGGGAGGATCGCTTGGGCCCAGGAGGTAGAGGT920.3525849844785958No Hit
GCTACTGGGGTAGCTGGCACTACTGAACAGCTTTAACTCTTCCAGACTTTCA920.3525849844785958No Hit
ATCACTGGGGTATCAGGAAATCTTTACTGAGAGACAGGAAAAGATAGAGCAG910.34875253899513275No Hit
GCTCAGGGGGGTAGATTTGCAATATAGTCTGTGAAAAACAATAACAACAACA910.34875253899513275No Hit
CATAGGGTAAACATGATAGTCCATAACCATTTTGAAATGCTGGGCAAACTAC900.3449200935116698No Hit
GTGTTGGGGCGATCTGTCATTGGGGTCGACTGACGGTAACGGGGCAGAGAGG890.3410876480282068No Hit
GCATATGGGCACACACAGAAAATCCTAAAAAATACAAACCAATAAAGCAACT890.3410876480282068No Hit
AGCGCGGGTGTTATCGTCAGGAGCTGTGCAAACTGATGTGTGAACGGCTAAA870.33342275706128083No Hit
TTGTTAGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG850.3257578660943548No Hit
GGTTATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC840.3219254206108918No Hit
AAATGCGGGTAATTTGAGGTGGAAGTGGAAAGTGGAAACAACAATTATGAAA840.3219254206108918No Hit
AGAACTGGGAAGCTTTCTGAATTGTACCTATGTCAGTTTCCAGGTTTTGATA840.3219254206108918No Hit
TATAGCAGGGAAGTGTGTGTGTCTGTGTGTGTGTGTGCACGTGCGTGTGTAT830.31809297512742885No Hit
GTTGATGGGGGCCCGGCTGAGCCGACATTGCCGGCGTCTTGGCGATTCGGCC810.3104280841605028No Hit
AAGTTAGGGCAAGCACAAATCTTTCCCACTAGCCATTTAATAAGTTAAAAAA810.3104280841605028No Hit
GCGAACGGGACCCCTTCCTCTTTCTCTCTCCTTTGAATCATCTCAATTTTAT800.3065956386770398No Hit
ATTTAAGGGATGGTAAGGCTACAATACTCAAGTAACAAGGTTTGGGACAATG790.30276319319357686No Hit
GCTCCGGGGAGTGCAGTATAGACATATATTTGTACATCTTTCATGTGCCTTA780.2989307477101138No Hit
CACACAAGGGATAAGATCTTTGTGATGGCAACTCATGGCTTGTTGTCTTCTG780.2989307477101138No Hit
GTATGGGGTTAGGAAAATAGGCACACTTTCAAAGAGAATGTTCTCTTACACA780.2989307477101138No Hit
GGCTACGGGTGGACAAGGTCTGATACATTGTCTTTGGATGAACAGGCTTCAT760.29126585674318783No Hit
TTAATAGGGTGGTAGGAAATAGATAATGATGTTTTTGACATTCAGTGGCATC750.2874334112597248No Hit
TGGTCTGGGACATCATTCACTTGGAAGGCGTGGTCACTAAATGTAAACCAGT740.2836009657762618No Hit
GCCCGCCTCAACTTCCGGAAGTGCTGAGATTGCAGGTGTGAGCCACTGCACC730.27976852029279886No Hit
AATTGTGGGAACCTGACTAACTGATGAAAGTGTTTCTCTCCTAAGGCCAGTC720.27593607480933585No Hit
CTACTGGGGTGACCAAACCAGCCTTCGGAGCGTTCTCTGTCCTACTTCTGAC720.27593607480933585No Hit
ACGCATGGGTGTAGATAATTGTTACACTATATTATTTAGGGAATCATGACAA710.27210362932587284No Hit
GAGTGGGGTGCAATGACAACTTGTTTCTGTTTTATTTAAAGTAACTGACATT700.26827118384240983No Hit
TGCATTGGGAGAAGTAAGGTGTATAAAACCATTGAGTACTAATAGACTGCGG670.25677384739202086No Hit
GTTAATGGGAGCCTACCCCTTCCTTGTACTATCCCTATGAGGCATAATTATA670.25677384739202086No Hit
GCCAAAGGGACCGGGCTCTTGATGGACTTGGCAGCCAATGAAAAGGCCGTTC660.25294140190855785No Hit
CGATAGGGGATAATTAAATTTCCTGAAAGAGTATTGTACTTTCTGAACCCAC640.24527651094163183No Hit
ATTTATGGGACCCAAGAATCTACAACGATGATAAGAACACCTATATTCGTTA620.23761161997470584No Hit
TTCTCTGGGAAAGTGTTATGGGATTCCCTAGTGAGGAAAGAATTGGTATCTC610.23377917449124286No Hit
GCGTACGGGAAGGTAATCTACCTTGGGATTACTAGATCATATGGTTTTTTAA610.23377917449124286No Hit
TGTTCAGGGTCGACCATCCATTTTTCTTTCTTATCAGAAACAGGAGAACTGG600.22994672900777985No Hit
ATTTGCGGGTGATTAAGTTAGCAGTTAAAAAATATAACTTGTGTAGCCAGAA600.22994672900777985No Hit
AGAAACGGGATTGCCTTTAAAAATCTGTTCCGTTTTTTTCCCAGGTACTTAA590.22611428352431687No Hit
ACAACAGGGAATACAACTTGGTTATGTGGAAACATGTTGCTCATAATAAATG590.22611428352431687No Hit
TAATGCGGGATGGTTAGTCAGTTATTATAAGTTACTCTTAAAATGTTTTATT580.22228183804085386No Hit
TGCAGAGGGTCAGCCATATTAATATACATTGACATAAAGACCTTTGTTTTAA580.22228183804085386No Hit
TAATACGGGATGGAATATTTTATCCAAGTCCTTTGTTATCATTAAATTATTC560.21461694707392787No Hit
AACACGGGGCGATCTACGAGAGGGATGTGATCTCCAAGTTAAAAAAAATTTT540.20695205610700185No Hit
ACCCAAGGGTGACTCAATGTAAATAAATCAATAAACATGATAAGCATCTTCA530.2031196106235389No Hit
CATGAGGGTATAACCTATTTTATTCTCTGCCAAACAATTTTATGACTGCTGT530.2031196106235389No Hit
AATCCAGGGTATGCATGTTCATTGCAACTTAAAAGCGTAAAAACCCCAAATG520.19928716514007586No Hit
GGGCCTGGGGGGGGACCTTGGAATGTATCCCCTGTGAATAATGGGGGCTACT500.1916222741731499No Hit
GAGTTCCGGGGTCTCGCATGGAAGAAGTCGATTAGGTTAGGAGTTCATAGTT500.1916222741731499No Hit
ATGGCTGGGCACGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAA490.18778982868968688No Hit
GTTAATGGGAACCTCACCACCTCTTGCTCAGCCTATATACCGCCATCTTCAG490.18778982868968688No Hit
GCATCATGGGAAGACCAGCCTGGCCAACATGGTGAAACCCCGTCTCTACTAA490.18778982868968688No Hit
TTCATGGGGTAGTAGAGAGAGGGTTTCACCGTGTTGGCCGGGCTGGTCTTGA480.1839573832062239No Hit
GCATATGGGGGGCCTGGTAATTAAAGATTGACCCCTGACCTAATTGGTTATG480.1839573832062239No Hit
AGTTTGGGGATTAGAAATTATTTGATCAATGCTTCATTTCATTAAAATATCA470.1801249377227609No Hit
CAATGCGGGCAAACAGCGGGCTGATAGCAAGGAGAGCCTCAAAGCCACTACC470.1801249377227609No Hit
TTAGCAGGGATATACTTGAATGCAACATTGTGTTTTATTTTCCAACTCTGCC450.1724600467558349No Hit
TGCCATGGGAGCTTGAATTTATTTTATTTATTTATTTTTCATGGTGGGGGGC450.1724600467558349No Hit
TAGCGTGGGGTTTATAATACACCCAAAGGATCAGAAACATCCCAGTGTCATT450.1724600467558349No Hit
TTCCCCTTGGGCATGGCCCAGCCAGGGGTCTCTGTCAAGTCCCTGGTGTCGT440.1686276012723719No Hit
TTCTATGGGGTGAATAACCTCCTGTAAATGAATTTTATAACAAAAATGTACT430.1647951557889089No Hit
TCAACCGGGTGATCATTTGTTCAGAAAAAAAGCCCCTGAACTGATTTTGTTA430.1647951557889089No Hit
CTATAAGGGTGCTTATAGCACTTTAAGTTATTCTAAAAATGAAATTATAAGC430.1647951557889089No Hit
TGCGCGGTGGCTGGGGATGCCTCACAATCATGACGGAAGGCAAGGAGGAACA420.1609627103054459No Hit
AACTTTGGGGCCATTTTAAATGCAAAGCAAGACTTGTGAATCATAGTGTCTC410.15713026482198292No Hit
CTCACCAGGGGACTTCAAGGGGAAGCTTGATGGCAGCTCCTCTGATGATATT400.1532978193385199No Hit
CGAATAGGGATGTTTTCATAGTGCTGAAATCTTTAATATTCCTTAATTTGAA390.1494653738550569No Hit
ATTATTGGGAATGAAATATCTTAAGTCTAGGGTACTTTTTCCTAGAATCCTG380.14563292837159392No Hit
TCTGCAGGGATCTCTCCTTTCAATGATTAAAATTACCCATAGCTTCTAGAAA380.14563292837159392No Hit
GAGGCGGGAGGAGCGCTTGAGCCCAGGAGTTTGAGGACAGCCTGGGCCGCAT360.13796803740466793No Hit
CCAGAGGGACTACAGGACCAGAAGAAGCATTTCGAAATGTATGCCTGGCATC350.13413559192120492No Hit
TATTTAGGGGCAGCTTTTAAAAGATTTTTTTTTTTTCTCTCAACACCATGAT350.13413559192120492No Hit
TCACTGGGGATAGCTGAAAGGTGAAAGCAACACAGAGGTCCATCAACTGATA350.13413559192120492No Hit
CTACTAGGGTGTCTACTATTTGCCAGTACCTTAAGTGCTAAATATATACAAA340.1303031464377419No Hit
TAGCAAGGGGTTCCTGATTCCATTTAGATCTTTATTGTTTTTCTTCATTTTT340.1303031464377419No Hit
GGATGGGGGCAAGAAATAGGAAACCCCCCAATGTCTGTTGAACAGCTATCAC320.12263825547081592No Hit
CTAATTGGGATCAACGGGTACAAACGAGTCCTGGCCTTGTCTGTGGAGACGG310.11880580998735292No Hit
CACTAAGGGAGCCTAGCATTTCCTAGAGAAGAAAAATTGTCAGCATTTTCTT310.11880580998735292No Hit
CATAAAGGGGGGCATGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGC310.11880580998735292No Hit
TACGAGGGGTAGCTATTTTTCTTGTTGCATCTTTTCTACTTCAGTACACTTG300.11497336450388992No Hit
AACAGGGGGGCCTGCTTGATGGATACAGAGTTTCCTTTGGGGGCGATGAAAG300.11497336450388992No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGCAAGCGA290.11114091902042693No Hit
ATCATGGGGGGCAGTCACAGACACTTGAGCACACGCGTACACCCAGACATCT290.11114091902042693No Hit
TGTTTTGGGGAGTATTACTTTGTACAGGTTGATCACTTTTTTTAGAGTGAAG290.11114091902042693No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCAAGCGAACTCGTATGC290.11114091902042693No Hit
GCAGCTGGGGGACCACAGGCACGCATCGCCGTGCCTGGCTAATCTTTACGTT290.11114091902042693No Hit
CTATCAGGGATTTCAGGAACACCTCTATCTTTGGTTTAAATCTTACTCTTAG290.11114091902042693No Hit
GATATCGGGAGAGTGGCTGGGGTTTGAACCTTTAGGATTTCACAGCCATGCT280.10730847353696393No Hit
CGATAAGGGATTGTCCCTACTCTCCAACCCACTGTCCTCAACCCTTATAGCA280.10730847353696393No Hit
TGCTAAGGGATAAAATAGACAAATAGAAAATGGTCCAATTAGTTTCCTTTTT280.10730847353696393No Hit
AACTGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA280.10730847353696393No Hit
GGCACAGGGGAGTAGCAAAGGATCGAAGGCGACTGTCGCTACAACCCTCCCC270.10347602805350092No Hit
AGATAAGGGATTAAAGTTTTGAACCAAATGTGTTAAAGCTTACGCTTTGCCA270.10347602805350092No Hit
ACACTTGGGGTGGGCATTATTATTTTTATTGTTTTGGTTATTATTCCTCATC270.10347602805350092No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)