FastQCFastQC Report
Sat 31 Dec 2022
SRR5845186.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845186.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199662
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGC10340.5178752091033847No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGCCGTCT10340.5178752091033847No Hit
ATTAGAGGGCAAGTACATGAAATATGAGAAGAGCTACCGCTTCCACTTGGGT3090.1547615470144544No Hit
ATTAAAGGGGGAGGAGAAAGATGGCGGCTACCTTACTAGCTGCTCGGGGAGC2940.14724885055744208No Hit
CCTAGGGGGAAATCCGTCCTTGCAGAAACAGAAGGAATTTTACAGAAGCTAC2850.14274123268323466No Hit
CAAACAGGGGACACAAGTTTATATAACAAACCTGCACATGTACCCCTGAACC2740.13723192194809228No Hit
GACTCAGGAAGACCAGAGCAGAGACAGGACACAAAGTCAAAAGAACTCAGTC2730.13673107551762478No Hit
CTTGACGGGGATCTAATCATGGGGAAAAATCCAAGATTGAGACAGAAATCAA2720.13623022908715732No Hit
TGTCTGGGCAATTGTGTTTACATTAATAGTGGTGTTTTTACCTATGAAATTA2710.13572938265668982No Hit
GGCCGCGGGGGTATTGAAAAGCTGTCAAGTTTATAGTGGCTGATACAAGTTT2620.1312217647824824No Hit
ACTTAGGGGGATTGGAATAAAATAAAGAAAACAAAATGCATCTCACATGCCA2600.13022007192154741No Hit
GCACCCAGGGTTAAAAAGTTATTGAAAAATGAGAAGTTACTCTCTTTTGGTC2570.12871753263014493No Hit
TCTTAAGGGCTCAGTGAAAATTCTTAGCAATAAACTAAAATCAGGAATTCTG2560.12821668619967744No Hit
TAAGGCGGGAACAGAGCCAGACTCTGTCTCAATAAAGGAAAAAAAACAGTTC2550.12771583976920994No Hit
CAATATGGGATATTGTTCAGGGCTATATATCTGTACAAACTTTAAGCAATTG2500.1252116076168725No Hit
ATTAGGGGGATCTGTGCCCGGGCTATGGAAGTAATATACAAATACCGGAAAT2480.12420991475593753No Hit
AATGTGGGTGTAAAACGTTTGGGAATCGTACACTTTCTAAGAAGGAAGATTT2460.12320822189500255No Hit
TATGATGGGGTATTTTGTGACCTTATGATTAGTTTTATCTTTCAACTGATTT2460.12320822189500255No Hit
GAACAGGGACTTCTGAAAAGAATTTTGTTGTTTCTCAGCTGTTTTCCAAAAG2460.12320822189500255No Hit
TAAAATGGGCATGCAGGAGTTACAGACACTGGTTTCTTGGAAAATGGAGAGA2460.12320822189500255No Hit
AAGATGGGGTGGGGTTCGAACCTGTGCGGGATACGCTGTTGTCGGTGCCAGG2450.12270737546453507No Hit
TCTCAGGGGTGAAAACTAAATTTTCTACTGTATTCCCAGCACGGGTGACTTC2440.12220652903406758No Hit
CATTGCGGGATGGAGTGGAAATTTGGAGTGACATCTGGGAGCAGCGAATTGG2440.12220652903406758No Hit
ATAATGGGGGTTTTGTCCTTCTGGAAGAGGATGTTTGCTAGATATTTTAGAA2430.12170568260360008No Hit
GAAGAGGGGAGTCAGGTGGGTGCCAGGCCCTGGCCGTGGCGAAAGAGCCGGC2430.12170568260360008No Hit
GGTTTTGGGATTACTTTTCTTCCTACATTTTCCTTTTGCTGATTCATTTTTC2410.12070398974266511No Hit
ACTTCTGGGAACTTTTGACTAGCATATGATTATTTGTGAGCTAGATCAACCT2390.11970229688173012No Hit
GTAAAGGGGATCTTCTCACATCTGTAACAACTGCTCCTTGCAGAACATTTCT2380.11920145045126262No Hit
TCAAAAGGGTTCACATTAACAGGGAAAATTAGCTGAAAATAATGTCCAATTA2370.11870060402079513No Hit
ACACCAGGTGTGTGGGAAGCTGCAGTGAGAAAACCAAATAATCCTGGTTTGA2360.11819975759032766No Hit
TTCAAGGGGAGCAAGACCCTGTCTCAAAACAAACAAACAAACAGAAATGTAT2360.11819975759032766No Hit
GCATCTGGGAACTCTTGGGCAAAAAAACAAAATTAAGACAGTAATTAAAAAT2350.11769891115986017No Hit
GATCAAGGGCTTGGAATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGA2350.11769891115986017No Hit
GCAACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2350.11769891115986017No Hit
CCATAAGGGAGTTCTTCTAACTAAGATGTTAAGCATTTGGCTTAAAGTGTAT2340.11719806472939268No Hit
TATTAGGGAAGATTCTCAGGTGATTTCTAAGAATCAGGGAAACAAAGAATAC2340.11719806472939268No Hit
TTCAGTGGGATGTGAAAACAATGTAAAGCTACACAATTAAAATAGTGTGGCA2320.11619637186845769No Hit
CTAGTTGGGATTCCATTACACAAATGTGGCTCCTTAATAAACTGCTCTAATA2310.1156955254379902No Hit
AGCTTAGGGTATAATGAAGAAGTTTGTAGCAGAAACCTGAGAATATAGGTAT2290.11469383257705522No Hit
GCAGGTGGGATACATGGTGACCTTTTCCACTTCCTTCTTATTTTTAGCATCC2270.11369213971612026No Hit
AAGCCTGGGCATCAAAGCAATCTTTGTGATATTACTTCGCTATTAAATAAAG2260.11319129328565276No Hit
TAGACCGGGTAAGCTTTCTGGGAAAGAGCAGAGTTTTACTTCTTTGGAAGTT2260.11319129328565276No Hit
GGACAGGGGAGACCTAGCACTGCCGAGCGTTGGCAAGATGGACTTCAGCTTG2260.11319129328565276No Hit
CTTCTAGGGTACTAATTGTGAAACACTGGAAATTAATGATGCAGACAACTTG2250.11269044685518527No Hit
TCTCGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2250.11269044685518527No Hit
TAATGGGCAGTGTTTACAAGTAATACCAGAAAGTTGCCAAACCCTTCTCTAT2250.11269044685518527No Hit
TAACATGGGGTAGATATGTGATTTTTTGAGACTGTATTTTTAGAGCTGAATT2250.11269044685518527No Hit
GCTCAAGGGGAAAAAAAGACTGATGTATGTATTCATAAGAACAGTTTAAGAT2240.11218960042471778No Hit
ATGTTTGGGGATCCGTAAATGATAACCACTGCAAATTTTTTCAGTATAAAAT2240.11218960042471778No Hit
GATACTGGGAAGGCACATGTGTGATGTCCATTTGCCATAATGCGTCAGGACA2230.11168875399425028No Hit
TCTGCAGGGCCTCGATTTCCTGGGCTCAAGCAATCCTCCCACCTCAGCCCCC2230.11168875399425028No Hit
CACACAGGGATGTACTTTAGGGACTTCCACAATTATGTTCTGATCAATCCTA2230.11168875399425028No Hit
GGACAAGGGGCAGCTGAGTGGGGGTTACGGAGGCGGCTACGGTGGCCAGAGC2220.11118790756378279No Hit
GCCTTGGGGAGAGACTACTCCCGGAGGATCGGAGGACCCCAGCCCCGCCAGC2220.11118790756378279No Hit
GTTAATGGGAGAGCTATGAAGCAGAATTCACAAACTTTAAACCAAGAACATC2210.11068706113331529No Hit
ACAACAGGGTCGGTGGGGGTGTGTTTGTATAAATCTTCAAGCGTAGTGTTAA2210.11068706113331529No Hit
TAATGTGGGTGGCAACGGGAGAGCAGTGTGCAGAATGTAATGCTCTTTTGTA2200.1101862147028478No Hit
AGCCAGGGCAGTATTTTTATATTTCTTACATTATCCTAATGATTGAAAACTC2200.1101862147028478No Hit
TTGCAAGGGGCGGCGCGAGGCGGGCGCTGCGGCGCCAGCAGCCCTGTGGTGG2200.1101862147028478No Hit
TAAGCAGGGAGCTTTTGGGAGCGTCCTTGAGCAAAGCGCCACGCCCCGCGAG2200.1101862147028478No Hit
GGCTACGAGTGAAAAGGCACCCTAATTTGGGATAGTGTGAAGAAACATAGTT2190.10968536827238032No Hit
TGCGCAGGGAAGAAAAACAAGACCAATAAGGTAATCGAATGATGTGAGATCA2190.10968536827238032No Hit
CTTGTCGGGGGATTAGGAGTCATGACATTTCCCCATGAGAGTTCTAACAATT2180.10918452184191284No Hit
CATTTAGGGTCATTAAAACTGAGAATAAGCTTCAACTCCAGCTGTCTGGCAC2180.10918452184191284No Hit
AGCTGAGGGCTTAAAAAATAAAGTAACTACATTCATCTCTATATATGTTCAC2180.10918452184191284No Hit
AGTAACGGGTATCGTCTGATTTCCATTCTGGGTGATGCCAGGAAAAAGCAAT2180.10918452184191284No Hit
TGTTGCGGGAAATCTAAGAGTTATTGACCTTAAAGAGGAGATAGAGAAAAAG2180.10918452184191284No Hit
GGGCCGGGGAGGAAGAGTAGTGTGCATTCAGACATTCGGAATTTTTGCTAGT2180.10918452184191284No Hit
AAATGGGGGGTCTAAGAGAATATACGCTTATGGAGCTGCTTGTGATGCACAT2180.10918452184191284No Hit
TGTTTAGGGCAGACTCCTAAAGCATTCCCACTCCACCACTCCAAAAGGTCAC2170.10868367541144534No Hit
ATGTGTGGGAGACAGTGACCCGGAAGTAGAAGTGGCCCTTGCAGGCAAGAGT2160.10818282898097786No Hit
CACCGTGGGTATGAAATGGGTACTTGGAGGATTTAAGTAACTTAGTCAAGGG2160.10818282898097786No Hit
CTTACTGGGCATTAATAAATAATTACAGTATATTAAATTTCCATTCCATTCC2150.10768198255051037No Hit
ATCCAAGGGAATTTAGGAATGCTCAATGTGCTGTTGATTCTTTCAATCCACA2140.10718113612004287No Hit
AATTAGGGGCGATCTTCGTTGGGGTATTAATCAAATGACTTAAGGGATAGGG2140.10718113612004287No Hit
CTTTTCGGGACAAATTGTGTGGTTTCTTGAGGATTGGACTCTTGATGGATTT2130.10668028968957538No Hit
GAGTTTGGGTCCAGGAGTATTGTCATTGAAAATGGAATCCTGCACCTAAAAG2130.10668028968957538No Hit
AGCACAGGGAGGGGCAGCAGAACCAAGGCAAATATTTCAGCTGGGCTATACC2130.10668028968957538No Hit
ACAATTGGGAGTGATTTTTATAGCATGCACTATAAAGGCAAAGTGAAGAAGC2130.10668028968957538No Hit
TAATGCGGGTAGCTGTCCCTAATCACAGATCCTAAGGTAATATAATATAATT2120.10617944325910789No Hit
ATCTATGGGCTGTGTCTGGGGTTCAGCTTCATCCCCAGTCACCAACTGCAGG2120.10617944325910789No Hit
ATCGTTGGGCTCAGTGTAATGTCCATTCAAGGAGTATTAAATGAGGATTTCC2110.10567859682864039No Hit
ACAGAAGGGAGATTTGGAGATGAAGTCATGGAATCTGCATTTAATAAATGTA2110.10567859682864039No Hit
AGGCATGGGATCTCAGAACTTAAACAAAAATTAGATGTCGTGCACGGACTGT2110.10567859682864039No Hit
TTCAGTGGGAAGCAAGTCCTGAGTGACCTACAAAGAGACTTAGACTCCCACA2110.10567859682864039No Hit
TTTACAGGGAACTAGGGGAAGGTATGTGTATCCCAGGGGCCAGGAATCTTAG2110.10567859682864039No Hit
GGTATTGGGAGAATTCTCCACCCCGTATCACCAGAATAAACATTCTTCTCAG2100.10517775039817293No Hit
CTACATGGGAGTGACAGTGCGCGTCTTACAACTAATTCCACTAGGCATGGGA2100.10517775039817293No Hit
CTTGCTGGGATGAGAGCCGAGGTTCAGTGGGCATTCGCCACGCATGTGACCT2090.10467690396770543No Hit
GTCATAGGGTACAGCCCTGCTGCTGGGGTTGATTTTGAACTGGAGTCATTTT2090.10467690396770543No Hit
ATATGCGGAGTGGACAAAGAACTTGTGGTCTTTTAAAACTGGGACTGATACT2090.10467690396770543No Hit
CTACATGGGGACCAGCCTGGCCAACATGGGGAAACCCCGTCTCTACTAAAAA2090.10467690396770543No Hit
CGAAAAGGGGTGTGGTTGATTTGTACCCTAGGTGATCTTTGGCTTCCTCAAG2090.10467690396770543No Hit
AATGTAGGGGAAAAAAAGTAGTGTTTTTTTGGGGAACAGTATAATGTCTCAG2080.10417605753723794No Hit
TGTTAAGGGCAGCAGTAGCTCATCAGAGTCCGAGATGACATCGGAGTCCGAG2080.10417605753723794No Hit
GTTTTGGGGTGAGATGATTTGACTGGCATGAGGCCCATCATTGGGAGGTAAA2080.10417605753723794No Hit
CTGTGCTCGGGCCCTAAATAAATCTAAAGTCTAAAGTTTTATTGATGTGAAA2070.10367521110677044No Hit
TAGCAGGGTGTACAAACTAGTTTTTATAGCTATCAACATTAGGAGTAACTTT2070.10367521110677044No Hit
ATTATGGGGAACAAATTTTGGAACATAAAAAGAAGATACTTAAAAAGGAGAA2070.10367521110677044No Hit
AAATGAGGGGGGCGCAGGCGCAAGCCGGCAAGATGGCGGCGGCTGGGGCTGG2070.10367521110677044No Hit
TAGCTTGGGATCACTGGGAAAACACAAAAAATTACACAACCCAGCAACAACA2060.10317436467630295No Hit
GTGGTTGGGGGACTAGCCGGGTTGTATTTGGAAACGCGGAGTGAGTTTTTCC2060.10317436467630295No Hit
ATTCGAGGGCATAGCAATCTCAACATATGTATGTTACATGCTTGTGTAACAG2060.10317436467630295No Hit
ATTTCAGGGAGAAGGCACAGGTGGCGAGATGAGCCGGGTACCAGCGTTCCTG2050.10267351824583545No Hit
TTAGGCGGGATCCTCCCCCAGAGCTGCCCCGTTCAGACCATGGATCCTGAGG2040.10217267181536797No Hit
ACATCTGGGGGAAGTCATTTGATAGAAAGATGAGATCGGAGACAATGTTGTG2040.10217267181536797No Hit
TCTTAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2040.10217267181536797No Hit
TTTGAGTGGGAAGGAAAATAATATTTACTTCCAAGAATGAAATTCAAGTTTT2040.10217267181536797No Hit
TGCTCCGGGTGATTAATCTGTATATAAGCCACATTGAAACATATTCAGGAAT2030.10167182538490048No Hit
GGCACTGGGGAAGTATCTAAAGACAGTATATTTGTGAAGATCTTACAGAAAC2030.10167182538490048No Hit
GTAAACGGGATGCGCGTTGCGCGCCGGACGCGGAACGTCTGCCGGTGTCCCC2030.10167182538490048No Hit
TCAGAAGGGAGGTGCTGGCTGCTGTCTACAAGGCTCTGAGTGACCACCACAT2020.10117097895443299No Hit
CTAACTCGGGTGCAAAATCCAGTTCTTCCAAAAGCCACTTTATTTAGGGTTT2020.10117097895443299No Hit
CTTGCTGGGCATCGAGTCTCCGCGCTGGAAGAACAGCAGTTCCTGATCATTC2020.10117097895443299No Hit
GGCTCAGGGATTGTGCCTTCATGGTAGACAATGAGGCCATCTATGACATCTG2010.1006701325239655No Hit
ACCGAAGGGAGGAGTACAAAGAAGTTATACCATGGAGAGTACTTTATGTGGC2010.1006701325239655No Hit
GCAGTGGGGGTAGATGAATCACTGTGATTATTGTTTTCAGTCGAAATACTTC2010.1006701325239655No Hit
TTTATAGGGCAGCACTGCCCCTCAGCAGGGATGACTCAGCAGGGATGACTCA2000.10016928609349803No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)