FastQCFastQC Report
Sat 31 Dec 2022
SRR5845156.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845156.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139420
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGCCGTCT17581.260938172428633No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGC16421.1777363362501794No Hit
GTAAGCGGGGTTTGGCTCTGTGTCCCCACTAAAATCTCATCTCAAATTGTAA3190.22880504949074737No Hit
AAGAAGGGATTACACCAACATTTATTTAAACAGAATCTATAAAAATCATATG2970.2130253909051786No Hit
AGTTTTGGGGCTTGATGGGGATGGCATTGAATCTGTAAATTACCTTGGGCAG2520.1807488165256061No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCATGATT2420.17357624444125663No Hit
CAGCCTGGGTGACAGAGTAAGACCCTGTCTTAAACAAACAAAAAATTAAAAA2200.15779658585568784No Hit
TATATTGGGCAGTCCCAGAATTGGCTTAGCACAGAGACTCTAAATGATAGTA2060.14775498493759864No Hit
GCTTCAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA2050.14703772772916368No Hit
GAACTAGGGTGAAGGTAGAGGTGACATAAAAGTAGGCTGTATATTCAAATCC2020.14488595610385885No Hit
TCACGTGGGGGAAATGTACATCTCTAGCCATCAACTAGTAACAAGAATGGCG1990.14273418447855402No Hit
AGTCAAGGGTGTGGCTTGAATGCCTTCCCCTCATCTTGCCATGCTTGGCTGT1990.14273418447855402No Hit
TCCGTGGGGGGTGTGACTTTGTTCCAGTAAACTTTCTTTATAGAATGGGAGA1980.14201692727011905No Hit
CTCTTGGGGGAGGAAAATTATAAGGTAAAATTTTCCTGTTTTTCCCCCTTTG1970.14129967006168412No Hit
ACCAAGGGGCTGGTAGGGCCTGCAAACCTGCCCTACCAAACCTGAAAGAGGG1960.14058241285324916No Hit
TTTTGCGGGCATTTATAATTCCAGTATTTGTTGTTATCTAGAAAATGGCTGA1920.13771338401950942No Hit
CTTGCAGGGGTAAGGGGCCAATTCTAAGTATTCTTCACGGCAGCCAGAAGTT1910.13699612681107445No Hit
ACAAAAGGGCCACCTCACCCTCTCCGCACACCTCTGGCTGTCTTTTTGTACT1910.13699612681107445No Hit
AATAGTGGGCAGCCTGGTTTTAATGATGTTGATATGTTTGACAGGTCTCATT1910.13699612681107445No Hit
ACGCTGGGGGATAGTATCTCACATCTCAATATTAATGTTGAATGTAAATGAC1900.13627886960263952No Hit
CTCTCAGGGACCCACAAATCAGGAGTGAGATGAATGTCGTGATTATTTTTTG1890.13556161239420456No Hit
TTTGTAGGGGCCTGAATTTGATACGCATGCAATGAGTACTTTTACAAGAAAA1890.13556161239420456No Hit
ATGCTAGGGTCCCTCAGCTTAAAAAAATGGGACTCCTAGGGCTATTTGCCTT1850.1326925835604648No Hit
AAGTACGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1850.1326925835604648No Hit
GGCGATGGGCCTGGAACTTGAGTTATTAAAATCATAGGAATAAAATTATGGG1840.13197532635202983No Hit
ACTTATGGGAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGAGCTTGA1830.1312580691435949No Hit
AGGCTGGGTGACTTGAATCCAGGAAGCCCATTTTCACACTAGCCCTCAGGCC1820.13054081193515996No Hit
ACTAGAGGGTACCACTGCCTCATACTTTCTTCTACATCAGAGAATTCATAAA1810.129823554726725No Hit
GCCGCAGGGGAATTCGAGGAAGATGATGTGAATCTTCCAATTTTATCTATTT1810.129823554726725No Hit
GACAGCGGGGACTGCTGTAAGGACTGAATGGGCACAGAGGCTGTGACTGCTA1810.129823554726725No Hit
CTGGTTGGGTACATATTTCATTCATTTTGCCAGCATACCATATAGACAAGTT1810.129823554726725No Hit
TTAACGGGGTAGGCAAGGGACTCTTGGTCTCCTACTTATCCCAGGGAGCCCT1800.12910629751829006No Hit
GATCCGGGGTAGCCAAGGTTAAGAACCAGTACCGTACGTACAAAAAAGTTGA1800.12910629751829006No Hit
TACGCAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1790.1283890403098551No Hit
TTAAAAGGGCTCCTCACTCACCTCCAAGCTGATCCGTTCGCACCCAACCACT1770.12695452589298523No Hit
AGATATGGGCTCTGACAGACACGGTAACTAAATGTGAAAAACCAATAAGCTG1770.12695452589298523No Hit
TGCTGGGGGAGGGAAAATAATACAGAGTTTAACATTTTTAGATTGAAAGACA1740.1248027542676804No Hit
CTTGTGGGGGGCATCAGCTAAATCTCTGTCATATTGTTTATATTTTTTGCAA1730.12408549705924544No Hit
GCCCATCTCGGCCTCCCAAAATGCTGAGATTGCAGGCGTGGGCCACCATTCC1730.12408549705924544No Hit
CTCAATGGGTGTTCTCTGTGGTCTGCCTGGGCTGGGGGTTAGGCAAGGCAAG1720.12336823985081051No Hit
GCGTATGGGGTCTTGGTTTGCAGTGGGATGTTACTATCTCATGGTCGGTCAT1710.12265098264237556No Hit
TATGCAGGGACCACCAAAGTTACTATGAAGCCTGCTGGGTTTGGAAACCTAG1710.12265098264237556No Hit
CTTTTAGGGAGCCTCTACAGTGGGACAGTAGGAGGCAGGCAAGGAAGAAAGA1710.12265098264237556No Hit
TGCCTTGGGGGAAACTTTCACAATGTCCGGAGCCCTTGATGTCCTGCAAATG1700.12193372543394061No Hit
GAGCAAGGGCAGCTCACCACAACTTCCGTCTCCCAGGTTCAAGTGATTCTCC1690.12121646822550566No Hit
GATTCAGGGATATGTCTGTAGAATTTTCATCTGTGTTAGCTGAATGAGCTAC1690.12121646822550566No Hit
GGCACTGGGATTAAATATAAGTGGACAAAGACTCCAGTTAAAAGATTAAAAT1690.12121646822550566No Hit
GCCACCGGGTGCGAAACTCGGCGGCCACGGCCGAGAAGTGGCGCTTCCGCCT1690.12121646822550566No Hit
CACTGTGGGAACACCACTCATGCAACATTCCAGCCAGGCATGGATAACCCAA1680.12049921101707071No Hit
TAGCAAGGGGGATCCCCAGGATCTCCAGGCTCTCCTGGATCCGTACCTAAAA1670.11978195380863577No Hit
AGCCTGGGTAACAGAGTGAGACTCCGTCTCAAATGAATGAATGAATAAATAA1670.11978195380863577No Hit
CAATAAGGGCATTAAAGTGGAATGAAATATGAAGATGCAGTAAAGTTCGTAA1670.11978195380863577No Hit
GCTTGTGGGATATGCTCTCCCAATGAACAAGACATGCAGTGAAGAAAGAAAC1670.11978195380863577No Hit
ATTTCAGGGCTGGGAAGAACTTAGCAAGGCCTGTTTGCTCAGATTTTTCTCT1660.11906469660020083No Hit
GTACTAGGGGTGGCCACAGTCAGTTTCTTCTGGGAGGAGAGCTTGACCCAGC1650.11834743939176588No Hit
ATCTAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1650.11834743939176588No Hit
GCTTGTGGGCGATCTTGCAAGGGGATCTAAAGAAGGGCTTGGTGGAAGGAAG1650.11834743939176588No Hit
ATGTAGGGGAGGGAAGTGTCTTACCTATTGCTTTTTTGCAGAGGATATTTTA1640.11763018218333093No Hit
GGTGCAGGGATCATATATCATATATGAATGTACATTAGTCAAATGGTGTGAG1640.11763018218333093No Hit
TCAATAGGGGTCACTGTCACTGTCCTCTTTGCCTTAGTGCTTTCAGGAGCCC1640.11763018218333093No Hit
CAGCCTTCCCAAAGTGCTGGCATTGTAGGTGTGAGCTACTGCACCTGGCCAA1640.11763018218333093No Hit
TAAGCTGGGAGTATCAATTATTTCTATATTTACCAAAACACCTACTACTTTA1640.11763018218333093No Hit
GGACTGGGGATAGCAAACGCTTTATAATTGGCATAGACATAAAGGATAAAAG1640.11763018218333093No Hit
AATCTAGGGGATGGGAGTTTGGTGGGTATTACCTCCAGTTAAGAGCCATTGA1630.11691292497489598No Hit
CTTACGGGTTAGGGTGGAATGAAAAAACCAAACAACAAGCAAGGGGATTTAT1630.11691292497489598No Hit
GTATATGGGACACTACGCTCTTCCGATCTAGTCTGGGCAGTGAAAATGTGGT1630.11691292497489598No Hit
TTGCAAGGGTACTATGTCTATGTAGGGAAAAAAACAAAACAAAAAATTGCAC1630.11691292497489598No Hit
AGCAAAGGGAGATAGGAGAAGAGAGAGATCGCTGTCTCGGGAGAGAAATCAC1630.11691292497489598No Hit
TGCGGAGGGGGAGGGTAATCCTGCTCTTAATCGTGTGAGATGGACCCATTCT1620.11619566776646106No Hit
AGCTTAGGGAACCACACATTATAAGGGGCCAAGAATAGTGCTTGCTGCTGCT1620.11619566776646106No Hit
TGTTCTGGGACCTCGTGATCTGCCCACCTTGGCTTCCCAAAGTGCTGGGATT1590.11404389614115623No Hit
TTCATAGGGGGGATCGTGACAAAACATACAGGAGCTGATAACCAAAATAGCC1590.11404389614115623No Hit
TTTAGGGGGATTACATTAAATCTCTAGATTACATTCAGCAGCATGGACATCT1580.11332663893272128No Hit
TGTTTGGGAACTGTAAAATAAATACATTTGAAAAAGGCACCCTCTCAACCTT1570.11260938172428633No Hit
CTGGCTGGGGGACGCTGCGGGGCGGGCGGACAGACTGCGGGACGGACGGTGG1570.11260938172428633No Hit
TGCCGCAGGGATTCTTTAACTCCAATCAATGGTGATAAATATATTTTATATC1560.11189212451585138No Hit
GATACAGGGATTTGAGGACATGGTGTTTTGTAACCTGTTGTTTTTTCTTAAT1560.11189212451585138No Hit
GTGGCTGGGTAATTATTACTAGACCTAAGAAATGAGATAGATGGCAACACAA1560.11189212451585138No Hit
GTGAATGGGAAGTATTCACCAGTTAATAGCACCAGGTGGGATAAAACTTTTG1550.11117486730741644No Hit
AAGCGGGTGTCTCAGGAGGAGGTCTGCGATTTGCTCCATGCCGCACCCTTCC1540.1104576100989815No Hit
TTCTATGGGGGGGCCCCTGATGCCAAGAGCTCTGGAGGTTCTTGAAAGAGGG1540.1104576100989815No Hit
TAGAGCGGGGATCTTAACACGGGCATAGCCAAGGAGGTGGGACTCCCTGGAG1540.1104576100989815No Hit
GATAAGGGGCCAGGCAGTGAGGAATCCTGAAACACCAGGAGAGGCGTCAGAA1540.1104576100989815No Hit
AATGCTGGGCAGATGAACATATGGCAGAGACTAGTTACAAGACTCCTACTGG1530.10974035289054655No Hit
TATCACGGGGCACAATCACAGCTCGCTGCAGCCTTGACCTCCCGGGCTTAGG1530.10974035289054655No Hit
TTGCAGAGGCATAGCCTGTAAGTACATTCTGGGTGACCTTGTCTTGTAGGAC1530.10974035289054655No Hit
ATAAGCGGGCTGTAAGTATCTACTGAGTCTCTCCATGTGCCAGATATTAGAT1530.10974035289054655No Hit
ACTGCCGGGAATTGAGTGCAATGTGGTATTAACTTAATTGAAAGTGATATGT1530.10974035289054655No Hit
TTCTTAGGGAGCCGCGCGCTGCAGGTGGTGCGCGCCCGCAAGCAGATCGTAG1520.1090230956821116No Hit
ACATGCGGGATTGTCAACTATGATTACAAAGTAGTCCATTCTCAAGGTAAAC1520.1090230956821116No Hit
GGCAGAGGGGCCATACTACTGTTGTGAATTTACTCAACAGATTATTTTCAAA1520.1090230956821116No Hit
AGCCGGGGGAAGTAAAAAAGATTTTTTAAAATTTTGATGAAGCCCAATTATG1520.1090230956821116No Hit
ATTCAGGGGTACTCTATAGCCTTGGTACCTTGAAAGTGTTTAAAGCACTCAG1520.1090230956821116No Hit
CCATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1500.1075885812652417No Hit
CCAGTAGGGAGGTTTCAGAGCGCAGCCATCGGTGCGCTGCAGGAGGCTAGCG1500.1075885812652417No Hit
TAGCGTGGGATCTGCTACTGCCCTTTCTATAATAACTAAAGTTAGCTGCCCT1500.1075885812652417No Hit
AGGCTTGGGGATGAAAACCATCGCCCCTTGAGTGCTGCATCCAACAGTGATC1490.10687132405680676No Hit
CTACAGCGGGATCTCTGAACATTTGCTTTTGGCATCTTCTTCAGTAGCCCAA1490.10687132405680676No Hit
GCCACGGGGAGCCCAGCCGGGCTTCAGTTCCATATTTGCCATGTGTCTGTCC1490.10687132405680676No Hit
AACAATGGGGCAGCCTTAAGAGCCGTAACACTCACTGTGAAGGTCTGCAGCT1490.10687132405680676No Hit
ATTACTGGGCAGCTACTTCTTCGAACAGTGATGCTGGAAACCCAGTGACAAT1480.10615406684837182No Hit
TCAGGCGGGTATACTGTGTGTTTAAAACATGTTTTAATTTTAGCAGCATCAT1480.10615406684837182No Hit
GCATCTGGGATTGTCTTAAGTATTATCTACTATGTATCTTTAACACTTTTGA1480.10615406684837182No Hit
TTTATTGGGCAACAAAAAGAGGCTAAGAATTGAGGCAAAGAAAAACCAAAAT1470.10543680963993687No Hit
AATGCTGGGATAATAGAATAGCCAAATGCATACTCTTCTTCTCACTAGAAGG1460.10471955243150195No Hit
CATCCAGGGGATCCCGCGGTCCCAGGACCCCCGGCAGCAGCTGCCACGCTCA1460.10471955243150195No Hit
GTATTAGGGAAGATCTGTAATACTTGGAGATAGGAACTATGTCACATAGTGC1450.104002295223067No Hit
ATAAATGGGGATTCTCAGAGCTACATATTATTTTACCGAAAAGCCATAACTA1450.104002295223067No Hit
ACACTCGGGCTGGGCTCAGGCTCTCCTCCTGCCTTAGCCCTCCAAGTAGCTG1450.104002295223067No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCATGATTA1450.104002295223067No Hit
TACAGCGGGAATGTCCATTTTACCTGTTAAATTTGTTGGCAGATATTTAAAA1440.10328503801463206No Hit
GCCGCAGGGATACTAGATAATATCTTACAAAGCTGTTATGTAGAGTAAACGA1440.10328503801463206No Hit
CACCATGGGAACTCTAACTTGTTGAATACTTGTTGACATACTTGTTGAATTT1430.1025677808061971No Hit
GTCTCCGGGACTGGCTTAATCACCGATTATCCACAGGGATGTTTACATTCAG1430.1025677808061971No Hit
TTCAAAGGGGATCCCTGAAAACTAATGTTATTTCCTAAAATTACTGTCCCTC1430.1025677808061971No Hit
GTCTCTGGGTCTAACGGCGGGTGATTGCTTTAAATTAAGCACAAGAGGCCAT1420.10185052359776216No Hit
TGCGAGAGGGCAGCTTTTCATATATGGTCTTTGTTATCTTGAAGTTTTTTCC1420.10185052359776216No Hit
GTTCAGGGGGGGGGCCAAGCTGCCAAGCTGACACTTAGCGAGCAAGTACGGA1420.10185052359776216No Hit
ACACTTGGGCATTCCTAGCTTGGGAAGCTTCCGCTTAGAGGTCCTGGCGCCT1410.10113326638932721No Hit
CATAAAGGGCAGGCACTTACTTTATGTCAAAAGAAAAATTAGGCATATCCAG1410.10113326638932721No Hit
AATCTAGGGTCAATTCCAAGTTAAGCAAGATTATATTGAATTCAGCTTTACT1410.10113326638932721No Hit
AACAGCGGGCAATTGAGATAAGTCTTGTCATGTATTGAATCTGTGCATTTTG1410.10113326638932721No Hit
CGCTAGGGGGAGAAGAATTAGAATCCTACAAGGGACACTTTGGTCCTATTCA1410.10113326638932721No Hit
AATAAAGGGAATCAAGGAGAAGTTTATGCAACTGAAAATGTTTACACACTGT1400.10041600918089227No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGATTATCCTGTCTCTTATAC1400.10041600918089227No Hit
TCAAGTGGGAATCATTGTTGAGGAATTACAGAGACAGTGAGATATGTCAGAA1400.10041600918089227No Hit
TACATCGGGCACCAACTTTAGTATTCTAAAGCACAGACTTTGTCATGTTATC1400.10041600918089227No Hit
CTTCAAGGGCAAATCCATCACACATTGGCTTATTTGAAACTTTTGGTTTTCC1400.10041600918089227No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)