FastQCFastQC Report
Sat 31 Dec 2022
SRR5845008.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845008.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167914
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGC4390.26144335790940604No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGCCGTCT3990.23762163964886787No Hit
ATCAGCGGGGGGAAGAAGAGATTGAAGCTCAAGAAAAAGCCAAACGGGAAAG2860.17032528556284765No Hit
CCAACTACTAGGTCTTGATTGTTCCAATTAATGATATATTTGATCTTTTGGA2710.16139214121514586No Hit
GTAGACGGGGATTTGAAGCTGGAGGTACAAAGTAAAAGTCCGCCAAACCCAT2550.1518634539109306No Hit
GTAAACGGGTAGCTTAAAAATGAGGGCATTTGCATGATTGAGGGATTGTGTA2470.14709911025882297No Hit
TTTACCGGGATGTTCACGATTCTTTGAGCACTTTACTACTTTGTGATGCGGG2460.1465035673023095No Hit
GCAACAGGGATTTGCAAGTTCAATTTGAATCTTTTTTTAGTCTGCTTGTACT2440.1453124813892826No Hit
ATACCAGGGTTTGGGAAGAAAAGTAATAACACAGCTACAAGTTAAATTGAAA2430.14471693843276914No Hit
GTTGCAGGGGTCCACGTCCATTGCTCAGCTCAAAGTGGTACAGACCAAGTAT2350.13995259478066152No Hit
GTTGCTGGGATACAAACCAAAATTTTAATCCATACAGGAACCAGAGTCATTA2320.13816596591112118No Hit
GACAATGGGGAAATCTTTTAGAAGTTTGTAGAATGGTAATATCAGAGTGCTG2300.13697487999809427No Hit
AAATGAGGGGTGAAGGCAGCCAAGAGTGAGGCTGTACCCTGAAAAGCCACAG2260.13459270817204044No Hit
GCCGACGGGATGCCAGTTAAATGAATGTATTTTCAGTTTACTTTCCATTGAT2260.13459270817204044No Hit
CATTCTGGGGGCGTGTGATGTGTGATTAGGCTTCTTGCTCTTAAATCCAGGT2240.13340162225901356No Hit
TGCCCCAGGTCCCATTGCTAGGAAACCCTTAAATTGTAGCAGCTCTTCTCTT2220.13221053634598665No Hit
TTGCTTGGGACATCTATCTTTACTAGAGGGCAGGATTTGCTATCTCTCTGGA2190.13042390747644628No Hit
TGTTTTGGGGGGAAAACGGGCAGGGGAAAGTCAGGCCCCACACTGTCTCCAG2190.13042390747644628No Hit
ATCAAGGGCGCTTTATAATTGGCATAGACATAAAGGATAAAAGGAAAATAAC2160.1286372786069059No Hit
TTGATGGGGATAGCAATCTTCTCAACAGAAGTAGGCATGGAAAAGACTTCTT2150.12804173565039245No Hit
TGTAGGGATTATTAGTTTTATGTGGTTTATATTTCAGGTAACATAAATTAAT2150.12804173565039245No Hit
TATTGCGGGGCCTCCTCCCTGTGCTCTGGGGATGCTGTTTTTTAAACACTTT2140.127446192693879No Hit
AATGCAGGGCGTTTGATTATTCTTATTTTTAAACCATGTGCAGTATCCCTTA2120.12625510678085208No Hit
ACAATGGGGATTCTACAGCAAAAAAGCATTAGTCTGTAATAATTTATGTGTA2120.12625510678085208No Hit
AGCTGGGGGGGCGCCGCCATGGCCGACGTGGAAGACGGAGAGGAAACCTGCG2110.12565956382433865No Hit
CTGAGGGAGAACTGAATTCCTCTGGAATTGAGTGATGGACTTCACAACAACT2110.12565956382433865No Hit
GTCGCGGGGGTGTGCAATTTTCTGCAACATTCCAGGTTAGAAAACTGCAATC2090.12446847791131174No Hit
TGCTCTGGGCTGACTTCTCCTCTCTAGCTGTGGAAGTCCTAGATGGCATATT2090.12446847791131174No Hit
AGTCATGGGGGGGAAGCAGAGTTTGAAGACGAGCCCCCTCCGCGGGAGGCTG2080.12387293495479829No Hit
TGTTGTGGGATTAATTGTGTTAAGTATCACTCTTTAACAAAGACCAGACCTA2080.12387293495479829No Hit
TTTGCAGGGTTCACATGTGCCAGTATGTCTAGCACATGTGAACCTTGCAAAA2070.12327739199828483No Hit
AAGTTCGGGTCTGTGGTTGAACCAAACTTTGTTATTTGGTGCATGACTGTAT2070.12327739199828483No Hit
GCAATGGAAATTTGTTTTGTCATATTTGCTTTCACTGTCTATTATCTGTTTA2070.12327739199828483No Hit
GCACAAGGGCAGTTTCTAATACAACTGACCCTTGAACAACATGGGTTTGAAC2060.12268184904177137No Hit
CTGATAGGGATCACATGGCAGTTCTCTTTTTAGTATTTTGAGGAACTTGTAT2060.12268184904177137No Hit
GTGCATAAGGGGGGCTGATTTGGGTCTATTTTGCACTTGTGTGGTTCAGGGG2060.12268184904177137No Hit
TAGACTGGGACTATGAAAAAAGCTGCAGACTGTGTAGACATATGTTTTCATT2060.12268184904177137No Hit
CTGTACGGGGCAGTGGCACCTCGTGCAAAATAATAAACTCATCTAAGATCCT2060.12268184904177137No Hit
CTTATCGGGGTTTTGATCCTCTTCAAAAGGTGGTTTATAATCAGCTGTAGAA2060.12268184904177137No Hit
TGCTGTGGGGGTTCTGTAACTGGTATGTTACAACTGAAAAACTTTTAAAATG2050.12208630608525793No Hit
GCCATGAGGAGGTTTGGCATAGAGCTGTGGTTTGCTTCCTGCATGCATTTGT2050.12208630608525793No Hit
AAGTTAGGGTACATTTTGGTTCAGTTTATTTCCATGTTGAATTAGAGTGGAT2050.12208630608525793No Hit
TACAATGGTGTCCTGGATCTGCCATTAAACAAGGAATATCCCTCCCCAGCTA2030.12089522017223102No Hit
GTAAAGGGGGAACCTCCGAGCAACACCTAAACAAAGGGAGTTGATGCCTCCG2030.12089522017223102No Hit
GCCTCAGGGAAATACAAATCAAAACCACAGTGAGATACCATCTCACAACAGT2020.12029967721571756No Hit
TTGCACGGGACCCTTTGTTTCACCCTGTCAGTCTCCAACCTGACGAGCAGAG2020.12029967721571756No Hit
GTCTTGGGGTATTGAGATCATGCCATTCTTGGTAATTTACAATTGACAGATA2010.11970413425920412No Hit
GATACTGCATGGGAGAGAGTTCTTCCCAAACGTTGATTCAGGAATTTTCCCC2010.11970413425920412No Hit
TTTCCAGGGGGAGGGTGAAATTTAGCCATCGGTGTGTGGCAGGGTCATGAAA2010.11970413425920412No Hit
AACGCAGGGTTAAAGGGAAATATTTTTGAAATGTTGATTGAATATTTATGAG2000.11910859130269066No Hit
TTAACTGGGGGCTGTCTTGAATGCTCAAATGCTGATTTTGTTTTCCTTCACA1990.11851304834617721No Hit
TTGCTGGGGTCTGCATTAGGGGGGTACCATCACATAATAATCACCAATAGTT1990.11851304834617721No Hit
AAGCCATGGGAGCAGACATGATCCTAGTGGATGATGATTTTCAAACCATAAT1980.11791750538966375No Hit
TTCAGTGGGGAGCGTAATAAAAACATGACTGAAAGTTTCTTCCTGAGCGGGG1980.11791750538966375No Hit
GTAAGCGGATGAACAAGAGATGTCTTTCTGTTTATTTGGATCTTTTTTCATT1960.11672641947663685No Hit
TTAGTAGGGCCCGTGTCTTTGCCAGCATTTGGTGTTGTGTTTTGAATTTAGC1960.11672641947663685No Hit
TAATTGGGGGAAGGAAAGTAATAGCTGCTTTTAATAGGTGGTTTGAACTAGC1960.11672641947663685No Hit
TAACCAGGGCATTTGGCAACCAAAATTACTCTAAGAATCATCACCCAAGTCA1950.1161308765201234No Hit
GCTACGGGGGAGAACTTTTTTTTAAGAACATGGGAAACTGAAAGATCATTTT1950.1161308765201234No Hit
CTGGCAGGGCATAGTGATATTTGCAAATTTGGACAACTTAAAAAGACTTGCA1940.11553533356360994No Hit
AGACGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1940.11553533356360994No Hit
CCGCGGGGCGTAGGGGTAGAGTAAGAATTCTGCTTGGTCAGAATCGTGATGG1940.11553533356360994No Hit
AAACTCGGGGTTGTTGAATTAATTTATCTTTTAGGATTCTGTTCGGTAAGAC1940.11553533356360994No Hit
TTGCAACCAAGCAAACAAACAAAAACCAAAAAACAAATAACAAACCAAGAAA1940.11553533356360994No Hit
ACTAGAGGCCCCCAGAGACGGTACAACCCAAGCTCCCACGTCCTCTCTGGAG1930.1149397906070965No Hit
AGCTCAGGGGAGATGGAGTCTTGCTCTGTCTCCCAGGCTGCAGTGCAATGGT1930.1149397906070965No Hit
TGTCAAGGGGTAAAATAAGTAAGTTGTGGTAAATTCATTAATAAAATGAGAA1930.1149397906070965No Hit
GCCCGCCTCCACCTCCCAAAGTGCTGGGATTACAGACCAAGAGCAGTGATGA1930.1149397906070965No Hit
AGCTAAGGGGCAGCAGCCCTAGGGGGTTGTCAGAATCACATTCTGGTTAAGT1920.11434424765058304No Hit
ATATGCGGGAACGTCACAAAATTGGGTAAGGAATTAAAGGAAGATTGCCTGG1920.11434424765058304No Hit
TGCAATGGGGGAACTAGGTAATTCACTCTTATGATAAAGAGCTATTAAAATG1910.11374870469406959No Hit
AATATAGGGAGCTCACTGCAAGCTCCGCCTCCCGGGTTCACGCCATTCTCCT1910.11374870469406959No Hit
CGCGGGAGGGGGCTAAATGACTGGGATATGAAGTAGGAATGGGAGAGGAAAG1910.11374870469406959No Hit
TAGCTTAGGGGGTCCTTCATTTGAGCAGCTTCCTTTATGACTCATCTTTCTA1910.11374870469406959No Hit
TACAATGGGGTGGTTTCAATCTAATTTTTTCCCAGACTACTATTTTCTTTTT1890.11255761878104269No Hit
GTGCGGGGGAAGGTGGTTTATTACAAATAAGCACACTAACCCCTCGGAAGTT1890.11255761878104269No Hit
TACGCAGGGAGCTGGGTTCTAAGCTTCACCATGCAAATCAGAAATTTTATCT1890.11255761878104269No Hit
TAAACGCGGGATGTACTATAATGATTTTGTTAAGTTTTCCCCATTATAGGGA1890.11255761878104269No Hit
TTGTCAGGGGGAAATGATGGCGACTGGAACGCCAGAGTCTCAAGCGCGGTTC1880.11196207582452923No Hit
GTTCTTGGGGCCTCGGAGCTGAAGCCCGTACTCAAGATGGCGGCTCCGGGCG1880.11196207582452923No Hit
GCACTTGGGAGTGTATTTGTACACTGATAAACTGATCTTCCGAAGAGACTGT1870.11136653286801577No Hit
GCTTTGGGGTACTAACAGTGCTTTCATGAAGACTCATTATTTTTCATCTGGA1870.11136653286801577No Hit
CAGTTAGGGAGGTGTTTTGAGGTGTTGGCATTCTTCGCTGATTTGGCTGTTC1870.11136653286801577No Hit
TCCAGTGGGGTTTTCTATGACGTATTTGGTTTTCTTCCAACCCAGTGGTGTT1870.11136653286801577No Hit
GATGCAGGGATGGTTCAACATATGCAAGTCAATAAATGTGATTCACCATGTA1860.11077098991150232No Hit
TGCAATTGGGGGGGCACGCTCCAGTATGCCAGTCCTGCGGGATTACGTCCAG1860.11077098991150232No Hit
CTCAATGTGGGCAGCCAATGGGCTATAAATTAGTGCTTCTAGAACTTTAATG1860.11077098991150232No Hit
TTCTCTGGGACGTCTTGGTCACTGTCATATTTTGAACCATCTCATCAGAGAT1860.11077098991150232No Hit
GCAGCAGGGCTTCAACTTTTTGGAAGAATTGGTGTAGAATTAGCATTACTTC1860.11077098991150232No Hit
AAGAATGGGTCTCTTTGGTCTGGCTGAGGGGAAGGAGGCAGAATCCCGATGT1850.11017544695498888No Hit
GCTCAGGGAAATGGTACATGTGTTGCTGTCTTGTCTAAAACTTTTAAAGGCA1850.11017544695498888No Hit
CTCACAGGGTGAAAACAATTTGCTTAAGCCTGCACAGCAGCTGTGCTGGCAA1850.11017544695498888No Hit
AAGCGGGGAAGAGGAGAGAAGGCAGCAAGAATTATGACTAATTCAAAACTGG1840.10957990399847542No Hit
TACTGCGGGGAGCAGACTGCTGCGGTTCCTGGGTGGCGGCGGCATGGAGGCG1840.10957990399847542No Hit
ATGCCCGGGATCTTTCCATGTTAGCAAGTCATTCTATGTCATCATCTTTACT1840.10957990399847542No Hit
CATTGCGGGCCGTGTTGGCCAGGCTGGTCTCGAACTCCTGACGTCAGGTGAT1840.10957990399847542No Hit
GATTAGGGAGAGCATAGCTCCTTTGGTCCTGGACCAGTTCCCAGAGGCAAAA1840.10957990399847542No Hit
AAACCGGGGGTTTTTGTAGGAAATGACAAACTAATTCTAAGATTCGTATGAG1840.10957990399847542No Hit
CAACCCGGGCAAGGTCTGCTTCTCATGCTGCAGCGACGGCAACATCGCTGTG1830.10898436104196196No Hit
CTCTTGCTGGGACACTAATGAAGAAGAAAAATATTGCCAAACAAAGTTGTTT1820.10838881808544851No Hit
TTAAAGGGGTTATAATCAAGCTACAAATAGGTTTTCTTAAACCAGAGATGCA1820.10838881808544851No Hit
TAGCCTGGGCAACAGAGCAAGACGCTATCTTGAAAAACAAAAAGAAGTGAAT1820.10838881808544851No Hit
GCAGTTGGGCATGTGATATTTTCACATCTTAATTAAGCTCCTTCAGTTTGAA1820.10838881808544851No Hit
TATCTGGGGGGCATCTTCCCGGAATCAGATTCAAAGGATCCTGGAGGTGATG1820.10838881808544851No Hit
GTCGATGGGCTGTGCGATTCTGTATCTTGGGAAAAGAGTCCTTTTATGACAC1820.10838881808544851No Hit
GACAGTGGGGAAAAGATTCTGGGTAATTGAGAGGCAGGTACTAGGAATAAAC1810.10779327512893506No Hit
GGCAATGGGATGTCAGATTCAAACATATTGAAATATTTTTTAGAGTATAAAA1810.10779327512893506No Hit
GCCGGGGGTAGTTGAATGACCTTGAGAAAGATTCTTAAAATCTTTTAGACCC1810.10779327512893506No Hit
GCAACTGGGGTTGGGGTGAAGCACACTTATTATCTTCAGTTGCAGTGATTTC1800.10719773217242161No Hit
AGCGAAGGGTAACCCAAGTGACGAAAACATTGTCCCCAATGAATATACTCAT1800.10719773217242161No Hit
ACGCATGGGGATATATTTGAAATTGTAGATTTCTATCTGTACAACATCTCCT1800.10719773217242161No Hit
ACTCCGGGATGTTCAATTGCCTTTGAAAGAGAGTAGTCCAGGTATATTCCTG1790.10660218921590815No Hit
TACTTAGGGTATGGTACTGGTGAAAGGATAGAAACATAGATCAATGGAACAC1790.10660218921590815No Hit
CACCCTGGATGAAAGGATGTTAAATATTTATTTTAGAAATGAGGAACTTTGA1780.1060066462593947No Hit
CTGTCAGGGACGTGTTGGTGTGGAGCAATGACCGAGTTATTCGCTGGATACA1780.1060066462593947No Hit
TGCTATGGGTGGAATCGAAGCGACCACGTCTGGAACAGGTTTCTGATTCTCA1780.1060066462593947No Hit
CTTGCCGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTG1780.1060066462593947No Hit
CTATCAGGGATTGCAGATGCGATTCTCATTTAGCTGTCAGCAGCAGCCTGGG1770.10541110330288123No Hit
TTCAAGCGGGATGACAGTATGGAATCTAAGCCTTCAGTCGACTCTGGTACTC1770.10541110330288123No Hit
CTATCAAGGGGGGAATATGACACAAAGGTGGAATAAACATTAATTTTGTGCA1770.10541110330288123No Hit
ACACTATATTAGACCAGACCACCATGTGTCATTGTGTGTGTGTGTGTGTGTG1760.10481556034636777No Hit
TCGGGTGGGCGATCTAGTGTCGGGATCAGGAAATGTTTTCTTAAGCTTTTCC1760.10481556034636777No Hit
GGACATGGGCAGAATGGTGGGGCAAGTACTTGGTGCAGTCGGAGCTTTACCT1750.10422001738985431No Hit
TTGTACGGGAAGGAGCACATTACTATTTTAGGACAAGTATGCATATCATTTT1750.10422001738985431No Hit
GTCGTTGGGATGATTAATCAAAAATTGTAGAATATAACAAGTTCGCGTAAAT1750.10422001738985431No Hit
ATTGCAGGGATAGTTTTAGTTGTCACACTCATCAGAGATTGCACACAACTAT1750.10422001738985431No Hit
GTTTAAGGGAGCGCCCCCAGGATTGAACGTGGGGAGCCCCAGGGCAGAAGCG1740.10362447443334087No Hit
AGAGTCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1740.10362447443334087No Hit
CTTGCTGGGGTTGAATTAGTGAATAAACAAACTAGGTCAAAAACAAATGCAG1740.10362447443334087No Hit
TATCCTGGGTGATTCAGTATTACAGTTAGAAGTGTAGATTTGTCAAAGTATT1730.10302893147682742No Hit
GTAGACGGGCAGCCGTGGCCCCGACTCTGGCCTGGTGCCCCTGGCCTACGTG1730.10302893147682742No Hit
AAAAATGGGATAATGATTCATGTATTGTTCGGTTGTCTCAAAATTCTGTTTT1730.10302893147682742No Hit
GTATTGGGTGTGAGTGGGGATTTGGCTTACATTCTATTTAAGAAATCCAATC1730.10302893147682742No Hit
GCAATAGGGTCTATATATCTGTGAAGAAAATAGAAGAGCTAATGAATATGAT1720.10243338852031396No Hit
GATACTGGGGATTCTAAAAGGAGCTTGGAAACCTTTCTTCTTTTTCATTTAA1720.10243338852031396No Hit
CTGCTTGGGGAGCCATGTTAGCATTACTGAAGGCAGAGCCAGCCTTGCATTT1720.10243338852031396No Hit
TCCCTGCAGCTATAGTTGAACAAGCAGTCTTTAAAAACTGCTGTGAAACACA1720.10243338852031396No Hit
CAGCTAGGGTATCACAATATTTCTACTTGGTTATTAGTGATATAGAAAAGTT1720.10243338852031396No Hit
GCGTAAGGGAGAAAACGAACATGATGAACACGATGAGAATAATGCTGAAGCT1720.10243338852031396No Hit
GCTGTGGGGGCTACATTGTTTGGATTTCTATAGCTTTGCAAAATAGTTTAAA1710.10183784556380052No Hit
GCTCTTGGGATCCTATTATATAATGGAGATGTAGACATGGCCTGCAATTTCA1710.10183784556380052No Hit
CTAAAGAAGGGATTATTGAGAAACACATTATCTGACTCTTCTTACCTCCAAC1710.10183784556380052No Hit
AAGATAGGGATGAAAAGCAAATGAGGAAGATATTTTATAGAAATAATTAAGC1710.10183784556380052No Hit
TACGAGGGGAACTCTGGAGAAAAAATAATTTGGTTGGTTGAGAATGTCTTAC1710.10183784556380052No Hit
TAAGTTGGGTCAGAATTGTTCTTTGAATCCTAAGGAACCTCTGTCAACAGTT1710.10183784556380052No Hit
GTTTGCCGGGGTTACATATGTACCACCATTTTCTTTATCCAATCCGTATTGA1710.10183784556380052No Hit
TCTTACGGGAGCCAAAATAAAGACCGCCTGTAATAATCTGTTAGAAACAGAT1710.10183784556380052No Hit
TGGATTGGGATAATTCAATAGAAGGAAACACCATTTCCCCAAAGAATTAGAA1710.10183784556380052No Hit
AATGAATGCTTAGAAGATATAACCGTAAAAGATGGACTTTCTCTCCAGTTTA1700.10124230260728706No Hit
TCTATGGGGTGCCAGGAGTTGCTAAGTGGTGGATCACAGACTCCAGTTCAGG1700.10124230260728706No Hit
GATATAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1700.10124230260728706No Hit
TGTAAGGGGAGCCCTACTTTATACAATAAGTAGGAGTTTTGATCATTATTTC1700.10124230260728706No Hit
TAGCAAGGCATGGACACTGCCAAAGACACCCTGGAGACCATCGTGAGAGAAG1690.1006467596507736No Hit
ACTCAAGGGAGACCTAGTTGTTTAGATTATCTGTCATTAGTAAATGTCTGTC1690.1006467596507736No Hit
ACGTGAGGGGTCTCGAATACATACTTCTCTCATTGGCTGTATAAAGGCGTTA1690.1006467596507736No Hit
GCGACGGGGTATCTGTTATCTTCTTGACTGTCTAACCAAAGTGATCCAAAGA1690.1006467596507736No Hit
CTTAGTGGGATGGTGAAATTAACCAGCTATTGGATCGTAGCTTTAAAGTTTA1680.10005121669426015No Hit
AAAAGGGATAACACATGATGTCATCAATAACCTACTGAAATTTATTCTACTT1680.10005121669426015No Hit
TAGGCTGGGGGCTAGATCCTTCCCATAGTATCATCTGTCCTCTGGAATGACT1680.10005121669426015No Hit
ATGATGGGGAACCAACTGGCAGTTTGCTAAGCATATCTACTGGTGTTGTTTC1680.10005121669426015No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)