FastQCFastQC Report
Sat 31 Dec 2022
SRR5845007.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845007.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168062
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGC4740.2820387714057907No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGCCGTCT4090.2433625685758827No Hit
GTAGACGGGGATTTGAAGCTGGAGGTACAAAGTAAAAGTCCGCCAAACCCAT2910.17315038497697277No Hit
GCAACAGGGATTTGCAAGTTCAATTTGAATCTTTTTTTAGTCTGCTTGTACT2710.16125001487546262No Hit
CCAACTACTAGGTCTTGATTGTTCCAATTAATGATATATTTGATCTTTTGGA2650.15767990384500957No Hit
GTAAACGGGTAGCTTAAAAATGAGGGCATTTGCATGATTGAGGGATTGTGTA2590.15410979281455653No Hit
ATCAGCGGGGGGAAGAAGAGATTGAAGCTCAAGAAAAAGCCAAACGGGAAAG2430.1445894967333484No Hit
TTGCAACCAAGCAAACAAACAAAAACCAAAAAACAAATAACAAACCAAGAAA2410.1433994597231974No Hit
AGCTGGGGGGGCGCCGCCATGGCCGACGTGGAAGACGGAGAGGAAACCTGCG2300.1368542561673668No Hit
GTAAAGGGGGAACCTCCGAGCAACACCTAAACAAAGGGAGTTGATGCCTCCG2270.1350692006521403No Hit
TTGATGGGGATAGCAATCTTCTCAACAGAAGTAGGCATGGAAAAGACTTCTT2230.13268912663183824No Hit
AAGCCATGGGAGCAGACATGATCCTAGTGGATGATGATTTTCAAACCATAAT2220.13209410812676273No Hit
TGCTGTGGGGGTTCTGTAACTGGTATGTTACAACTGAAAAACTTTTAAAATG2190.13030905261153622No Hit
AGCTAAGGGGCAGCAGCCCTAGGGGGTTGTCAGAATCACATTCTGGTTAAGT2190.13030905261153622No Hit
TTGTCAGGGGGAAATGATGGCGACTGGAACGCCAGAGTCTCAAGCGCGGTTC2180.1297140341064607No Hit
GTTGCTGGGATACAAACCAAAATTTTAATCCATACAGGAACCAGAGTCATTA2160.12852399709630968No Hit
ATACCAGGGTTTGGGAAGAAAAGTAATAACACAGCTACAAGTTAAATTGAAA2150.12792897859123417No Hit
TGTCAAGGGGTAAAATAAGTAAGTTGTGGTAAATTCATTAATAAAATGAGAA2130.12673894158108318No Hit
GCCTCAGGGAAATACAAATCAAAACCACAGTGAGATACCATCTCACAACAGT2090.12435886756078113No Hit
TGTAGGGATTATTAGTTTTATGTGGTTTATATTTCAGGTAACATAAATTAAT2090.12435886756078113No Hit
TTTACCGGGATGTTCACGATTCTTTGAGCACTTTACTACTTTGTGATGCGGG2070.12316883055063013No Hit
GTTGCAGGGGTCCACGTCCATTGCTCAGCTCAAAGTGGTACAGACCAAGTAT2060.12257381204555462No Hit
ATATGCGGGAACGTCACAAAATTGGGTAAGGAATTAAAGGAAGATTGCCTGG2060.12257381204555462No Hit
CATTCTGGGGGCGTGTGATGTGTGATTAGGCTTCTTGCTCTTAAATCCAGGT2050.12197879354047911No Hit
GTATTGGGTGTGAGTGGGGATTTGGCTTACATTCTATTTAAGAAATCCAATC2050.12197879354047911No Hit
AATGCAGGGCGTTTGATTATTCTTATTTTTAAACCATGTGCAGTATCCCTTA2040.1213837750354036No Hit
AAAAATGGGATAATGATTCATGTATTGTTCGGTTGTCTCAAAATTCTGTTTT2040.1213837750354036No Hit
TTAAAGGGGTTATAATCAAGCTACAAATAGGTTTTCTTAAACCAGAGATGCA2030.12078875653032808No Hit
AAGTTAGGGTACATTTTGGTTCAGTTTATTTCCATGTTGAATTAGAGTGGAT2030.12078875653032808No Hit
CTGATAGGGATCACATGGCAGTTCTCTTTTTAGTATTTTGAGGAACTTGTAT2020.12019373802525257No Hit
CTTGCTGGGGTTGAATTAGTGAATAAACAAACTAGGTCAAAAACAAATGCAG2010.11959871952017707No Hit
GCAACTGGGGTTGGGGTGAAGCACACTTATTATCTTCAGTTGCAGTGATTTC2000.11900370101510158No Hit
CAGCTAGGGTATCACAATATTTCTACTTGGTTATTAGTGATATAGAAAAGTT2000.11900370101510158No Hit
AAATGAGGGGTGAAGGCAGCCAAGAGTGAGGCTGTACCCTGAAAAGCCACAG1990.11840868251002606No Hit
TACTGCGGGTTGATACTTACCATAGATTGAGATTTTTGCAGTTCGGGTTGCT1990.11840868251002606No Hit
TCCCTGCAGCTATAGTTGAACAAGCAGTCTTTAAAAACTGCTGTGAAACACA1990.11840868251002606No Hit
TTTGCAGGGTTCACATGTGCCAGTATGTCTAGCACATGTGAACCTTGCAAAA1990.11840868251002606No Hit
CACCCTGGATGAAAGGATGTTAAATATTTATTTTAGAAATGAGGAACTTTGA1980.11781366400495055No Hit
TATGGCGGGCTGCAACCGGGAGGGAAGGTAAAAACAGGGACCAGCAATGTCA1980.11781366400495055No Hit
GATACTGCATGGGAGAGAGTTCTTCCCAAACGTTGATTCAGGAATTTTCCCC1980.11781366400495055No Hit
GACAATGGGGAAATCTTTTAGAAGTTTGTAGAATGGTAATATCAGAGTGCTG1970.11721864549987504No Hit
GCTGTGGGGGCTACATTGTTTGGATTTCTATAGCTTTGCAAAATAGTTTAAA1970.11721864549987504No Hit
CTATCAGGGCGTCTTGAAAATAAATGTGTTTAGAATTTTCTAAGAGAGAATG1970.11721864549987504No Hit
CTTGCCGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTG1970.11721864549987504No Hit
AACGCAGGGTTAAAGGGAAATATTTTTGAAATGTTGATTGAATATTTATGAG1960.11662362699479953No Hit
TTCAGTGGGGAGCGTAATAAAAACATGACTGAAAGTTTCTTCCTGAGCGGGG1960.11662362699479953No Hit
GATGCAGGGATGGTTCAACATATGCAAGTCAATAAATGTGATTCACCATGTA1950.11602860848972402No Hit
TGCTCTGGGCTGACTTCTCCTCTCTAGCTGTGGAAGTCCTAGATGGCATATT1950.11602860848972402No Hit
TTGCTTGGGACATCTATCTTTACTAGAGGGCAGGATTTGCTATCTCTCTGGA1950.11602860848972402No Hit
GTGCATAAGGGGGGCTGATTTGGGTCTATTTTGCACTTGTGTGGTTCAGGGG1950.11602860848972402No Hit
TTGCTGGGGTCTGCATTAGGGGGGTACCATCACATAATAATCACCAATAGTT1950.11602860848972402No Hit
ATCAAGGGCGCTTTATAATTGGCATAGACATAAAGGATAAAAGGAAAATAAC1950.11602860848972402No Hit
TGGATTGGGATAATTCAATAGAAGGAAACACCATTTCCCCAAAGAATTAGAA1950.11602860848972402No Hit
GCTCAGGGAAATGGTACATGTGTTGCTGTCTTGTCTAAAACTTTTAAAGGCA1940.11543358998464852No Hit
GCACTTGGGAGTGTATTTGTACACTGATAAACTGATCTTCCGAAGAGACTGT1920.11424355297449751No Hit
TACAATGGGGTGGTTTCAATCTAATTTTTTCCCAGACTACTATTTTCTTTTT1920.11424355297449751No Hit
CAACCCGGGCAAGGTCTGCTTCTCATGCTGCAGCGACGGCAACATCGCTGTG1920.11424355297449751No Hit
GTCGTTGGGATGATTAATCAAAAATTGTAGAATATAACAAGTTCGCGTAAAT1920.11424355297449751No Hit
ATTGCAGGGATAGTTTTAGTTGTCACACTCATCAGAGATTGCACACAACTAT1920.11424355297449751No Hit
GTCGATGGGCTGTGCGATTCTGTATCTTGGGAAAAGAGTCCTTTTATGACAC1920.11424355297449751No Hit
CTGGCAGGGCATAGTGATATTTGCAAATTTGGACAACTTAAAAAGACTTGCA1910.113648534469422No Hit
AAAAACGGGGTTGGCGGCGGATTCGAACGTTCGGACTGAGGTTTTTCTGCCT1910.113648534469422No Hit
CCGCGGGGCGTAGGGGTAGAGTAAGAATTCTGCTTGGTCAGAATCGTGATGG1910.113648534469422No Hit
AAACCGGGGGTTTTTGTAGGAAATGACAAACTAATTCTAAGATTCGTATGAG1910.113648534469422No Hit
AGTTGCGGGACATGCACACGTATGTTTATTGCAGCACTGCTCAGAATAGCAA1900.11305351596434648No Hit
TGCAATTGGGGGGGCACGCTCCAGTATGCCAGTCCTGCGGGATTACGTCCAG1900.11305351596434648No Hit
CTTTCGGGGTAGTCCTGAGACCCTTCGCCCCCTTTTCCTGCACCTAGTGGCC1890.11245849745927097No Hit
ATATATGGGAATGCTGCTTAGTTTATCTATAAACCACGGTTATACCCCAGTG1890.11245849745927097No Hit
AGCGAAGGGTAACCCAAGTGACGAAAACATTGTCCCCAATGAATATACTCAT1890.11245849745927097No Hit
CTTATCGGGGTTTTGATCCTCTTCAAAAGGTGGTTTATAATCAGCTGTAGAA1890.11245849745927097No Hit
CTGAGGGAGAACTGAATTCCTCTGGAATTGAGTGATGGACTTCACAACAACT1880.11186347895419546No Hit
GTCGCGGGGGTGTGCAATTTTCTGCAACATTCCAGGTTAGAAAACTGCAATC1860.11067344194404447No Hit
TGTTTTGGGGGGAAAACGGGCAGGGGAAAGTCAGGCCCCACACTGTCTCCAG1860.11067344194404447No Hit
TGCTAAGGGGGAGTATTTTCATATTCCAATACCGATAAATCTTTGAGGTTTC1860.11067344194404447No Hit
GCCAAGGGGTTGCATTATAGAAAGAGCCTCAAGATAGAATCAACAAGATTTC1850.11007842343896895No Hit
ATGCTAGGGTAACTCATTAGACGTACTGAGGCAGCACTTCTGCAGGGTGCAT1840.10948340493389344No Hit
TCTGTAGGGTAAGTAGTTGTGTTCATTTTTAAGTAAATAGTAGTAAACAGTT1840.10948340493389344No Hit
GTAAGCGGATGAACAAGAGATGTCTTTCTGTTTATTTGGATCTTTTTTCATT1840.10948340493389344No Hit
ACTAGAGGCCCCCAGAGACGGTACAACCCAAGCTCCCACGTCCTCTCTGGAG1840.10948340493389344No Hit
TACGCAGGGAGCTGGGTTCTAAGCTTCACCATGCAAATCAGAAATTTTATCT1840.10948340493389344No Hit
GCAATGGAAATTTGTTTTGTCATATTTGCTTTCACTGTCTATTATCTGTTTA1840.10948340493389344No Hit
TTGTACGGGGCTGTAACTTTTGACTAAAGAGCCTATATTTATCTAGTTAATG1830.10888838642881793No Hit
ACTCCGGGATGTTCAATTGCCTTTGAAAGAGAGTAGTCCAGGTATATTCCTG1830.10888838642881793No Hit
GCTTTGGGGTACTAACAGTGCTTTCATGAAGACTCATTATTTTTCATCTGGA1830.10888838642881793No Hit
CTGTACGGGGCAGTGGCACCTCGTGCAAAATAATAAACTCATCTAAGATCCT1830.10888838642881793No Hit
TCGGGTGGGCGATCTAGTGTCGGGATCAGGAAATGTTTTCTTAAGCTTTTCC1820.10829336792374242No Hit
TATTGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1820.10829336792374242No Hit
GCACAAGGGCAGTTTCTAATACAACTGACCCTTGAACAACATGGGTTTGAAC1810.1076983494186669No Hit
GTAGACGGGCAGCCGTGGCCCCGACTCTGGCCTGGTGCCCCTGGCCTACGTG1810.1076983494186669No Hit
GCCGACGGGATGCCAGTTAAATGAATGTATTTTCAGTTTACTTTCCATTGAT1810.1076983494186669No Hit
AGCTCAGGGGAGATGGAGTCTTGCTCTGTCTCCCAGGCTGCAGTGCAATGGT1800.10710333091359142No Hit
TGTTGTGGGATTAATTGTGTTAAGTATCACTCTTTAACAAAGACCAGACCTA1800.10710333091359142No Hit
TTAACTGGGGGCTGTCTTGAATGCTCAAATGCTGATTTTGTTTTCCTTCACA1800.10710333091359142No Hit
CTCAATGTGGGCAGCCAATGGGCTATAAATTAGTGCTTCTAGAACTTTAATG1800.10710333091359142No Hit
TCCAGTGGGGTTTTCTATGACGTATTTGGTTTTCTTCCAACCCAGTGGTGTT1800.10710333091359142No Hit
AAGAATGGGTCTCTTTGGTCTGGCTGAGGGGAAGGAGGCAGAATCCCGATGT1790.10650831240851591No Hit
GTAATGGGGTGCTGCTTGTTGCAGTGACTAACCACCAATGTTAACATCTTCA1790.10650831240851591No Hit
GGCTTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1780.1059132939034404No Hit
CATTGCGGGCCGTGTTGGCCAGGCTGGTCTCGAACTCCTGACGTCAGGTGAT1780.1059132939034404No Hit
GCGAATGGGTGATAACGGAATGGTTGCAATTAAGGACATTTTTTTTCCTCTT1780.1059132939034404No Hit
GGCAATGGGATGTCAGATTCAAACATATTGAAATATTTTTTAGAGTATAAAA1770.10531827539836489No Hit
TGAGGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1770.10531827539836489No Hit
AGAGTCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1770.10531827539836489No Hit
AAGTTCGGGTCTGTGGTTGAACCAAACTTTGTTATTTGGTGCATGACTGTAT1770.10531827539836489No Hit
TTGGTGGGGCTTATTTAACTCTAGGCATGTTGACTTTTCAAAGCGGTTTCCT1770.10531827539836489No Hit
TAATTGGGGGAAGGAAAGTAATAGCTGCTTTTAATAGGTGGTTTGAACTAGC1770.10531827539836489No Hit
GCTACGGGGGAGAACTTTTTTTTAAGAACATGGGAAACTGAAAGATCATTTT1760.10472325689328937No Hit
TAGGCTGGGGGCTAGATCCTTCCCATAGTATCATCTGTCCTCTGGAATGACT1760.10472325689328937No Hit
TAGCCTGGGCAACAGAGCAAGACGCTATCTTGAAAAACAAAAAGAAGTGAAT1760.10472325689328937No Hit
TATCCTGGGTGATTCAGTATTACAGTTAGAAGTGTAGATTTGTCAAAGTATT1750.10412823838821386No Hit
CACTGTGGGATCTACAAGATGAGACAAACAGATCGGGATGTAATAACAGCAT1750.10412823838821386No Hit
TCGATGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1750.10412823838821386No Hit
AAGATAGGGATGAAAAGCAAATGAGGAAGATATTTTATAGAAATAATTAAGC1750.10412823838821386No Hit
GTTTAAGGGAGCGCCCCCAGGATTGAACGTGGGGAGCCCCAGGGCAGAAGCG1750.10412823838821386No Hit
TCGGTAGGGAAGAACGGCTAATGGCAATAGGATCTTTAGCAACTTTTTCACA1750.10412823838821386No Hit
TTGCACGGGACCCTTTGTTTCACCCTGTCAGTCTCCAACCTGACGAGCAGAG1750.10412823838821386No Hit
ATGTGAGGGCTGGAGTTACCTGCCAGAATGGCCTCTTAATTAACTCAGGTAA1750.10412823838821386No Hit
TGCCCCAGGTCCCATTGCTAGGAAACCCTTAAATTGTAGCAGCTCTTCTCTT1750.10412823838821386No Hit
TAGCTTAGGGGGTCCTTCATTTGAGCAGCTTCCTTTATGACTCATCTTTCTA1750.10412823838821386No Hit
TAACCAGGGCATTTGGCAACCAAAATTACTCTAAGAATCATCACCCAAGTCA1740.10353321988313835No Hit
ACTGCAGGGGAGGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1740.10353321988313835No Hit
TAAGTTGGGTCAGAATTGTTCTTTGAATCCTAAGGAACCTCTGTCAACAGTT1740.10353321988313835No Hit
TACTTAGGGTATGGTACTGGTGAAAGGATAGAAACATAGATCAATGGAACAC1740.10353321988313835No Hit
TATCTGGGGGGCATCTTCCCGGAATCAGATTCAAAGGATCCTGGAGGTGATG1740.10353321988313835No Hit
GTAATTGGGATAATAAGAAAGAAGAAAAAGAAAGAAAAGTGGTAGCATTTTT1730.10293820137806287No Hit
TATTGCGGGGCCTCCTCCCTGTGCTCTGGGGATGCTGTTTTTTAAACACTTT1730.10293820137806287No Hit
ACGAGGGAGAATGTCTACCTTGTGAAGATGCATTGACCGATCCTTAGAGAAA1730.10293820137806287No Hit
CTCACAGGGTGAAAACAATTTGCTTAAGCCTGCACAGCAGCTGTGCTGGCAA1730.10293820137806287No Hit
CAGTTAGGGAGGTGTTTTGAGGTGTTGGCATTCTTCGCTGATTTGGCTGTTC1730.10293820137806287No Hit
TTTCCAGGGGGAGGGTGAAATTTAGCCATCGGTGTGTGGCAGGGTCATGAAA1730.10293820137806287No Hit
TCCTTTGGGATGGCTTTTTTTTGTGTTGAGGTATGTTCCTTCTATACCCAGT1720.10234318287298735No Hit
GTTCTTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1720.10234318287298735No Hit
AGCTTTGGGGCCTCCTGGTGTCGCACATCCATGGGAATAATGAATTTATTTG1720.10234318287298735No Hit
ATGCCCGGGATCTTTCCATGTTAGCAAGTCATTCTATGTCATCATCTTTACT1720.10234318287298735No Hit
ATTACAGGGCTCCCTCTGATGGTAAAAATATTGAACTTCAGACAGACACCTA1720.10234318287298735No Hit
ACGTGAGGGGTCTCGAATACATACTTCTCTCATTGGCTGTATAAAGGCGTTA1720.10234318287298735No Hit
TGATGAGGGAAGTTGGTCACCCTAAATTCATATAATATTAGTCTTCTACTCA1710.10174816436791184No Hit
ACTCAAGGGAGACCTAGTTGTTTAGATTATCTGTCATTAGTAAATGTCTGTC1710.10174816436791184No Hit
GTTCTTGGGGCCTCGGAGCTGAAGCCCGTACTCAAGATGGCGGCTCCGGGCG1710.10174816436791184No Hit
CTGCTTGGGGAGCCATGTTAGCATTACTGAAGGCAGAGCCAGCCTTGCATTT1710.10174816436791184No Hit
AACGTGGGAAAGGATTACTTTTACTTAAATAAATTCTTACCACTTTATGTGC1710.10174816436791184No Hit
AGACGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1710.10174816436791184No Hit
GAGCTGGGATAAGTTTAGGAGTCAGACTGCCATTTTTTTATGAATGTTAATA1710.10174816436791184No Hit
GTAACTGGGGATTGCATAGTGATGGAATCTGGACTTTTAGTATACCCATTGC1710.10174816436791184No Hit
GTCTCAGGGATGGTATACCAGATACAGATGGTTGCAAGGAAACCTTTTCCCC1700.10115314586283633No Hit
GAATAAGGGGCATTAAATTGATAAAGGCTATGAGATACACCTGCTCTCAACC1690.10055812735776082No Hit
TGCAATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1690.10055812735776082No Hit
CGCGGGAGGGGGCTAAATGACTGGGATATGAAGTAGGAATGGGAGAGGAAAG1690.10055812735776082No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)