FastQCFastQC Report
Sat 31 Dec 2022
SRR5845002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120536
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGC5780.4795247892745736No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGCCGTCT5610.465421119001792No Hit
GTAGCCGGGCTAGTGAAGGGATGTCAAGAAATTACATTTTGCATGTCTTATG1670.13854781973850136No Hit
AGCATCGGGGACGCTCTTTGATCTAGCTATGGGGTGAAACATGAGGCAAGTT1660.13771819207539657No Hit
CTTAAGGCGGAGTCACAATCCAGGGACTTGGGTATACTGCAAGCTGAGATTT1640.13605893674918695No Hit
AGGTTCGGGTATATTTAAATCCAAGAGAAACTATGCCGAAAAAAAGTGTTAT1620.13439968142297737No Hit
GCTGCGGGGAAATTTGGGTGTTTTTAAAACCTTGTTTGGGGTTGTTCCTGTC1610.13357005375987258No Hit
TTTTCAGGGACAGCTTTCTGTAGCAGAGATCACCAATGCTTGCTTTGAGCCA1600.13274042609676778No Hit
ATAAGCGGGCGATCTTGCTTAGGGATCCTAGAGAGAAATCAAAATAATTGAG1560.12942191544434856No Hit
GGAGTGGGGGGTTTCAAAAAACATTTTTCCAAATGTACTATTAGAAATAAAT1560.12942191544434856No Hit
TATATTGGGGACTTTGGCAGAGACTGACTTTTTAAATCACTAGAGCCTAGCA1550.1285922877812438No Hit
CCTAATGGGGAATCTGGCTGCTGACTTGGCACACAACTTCACTGATGGTCTG1550.1285922877812438No Hit
GGATTTGGGGAGATCAGTCTGTAAAATGGTTTCACAAGAATGTGAAACTATT1550.1285922877812438No Hit
TAACTTGGGCAGCTCACTGCAACCTCTGCCTCCCGGGTTCAAGCAATTCTCC1510.1252737771288246No Hit
ATGCAGGGGCGGCGGCGGAAGTGGCCGGCGAGCCCGGTCCCCGCCGGCACCA1500.12444414946571977No Hit
AAGTGTGGGAATGGATGCACGGTGAATGCATGAATGATTGAACGACAGAAAA1500.12444414946571977No Hit
AAGCCGGGCGATCTTGCTTAGGGGTATTTTTTTAAGTTTCAAACTCCATTCT1490.12361452180261498No Hit
TCACGGGTCTAGAAGCGGGAGATAAGTTTTTTTCTCTTTGAAAGATAGAGAT1490.12361452180261498No Hit
TTACAAGGGAAGGGGCCAATTCTAAGTATTCTTCACGGCAGCCAGAAGTTAA1470.1219552664764054No Hit
GTTGCCGGGATATTAGTTTAAGAAAAACAAAGTATGGGTGGGAGGAAAGACA1450.1202960111501958No Hit
TCTTTAGGGGCAGTGAGTTATTTCCCAAGTGGTAAGTAAACCTTGGAAGAGA1440.11946638348709099No Hit
AAAGATGGGTCTATATGGAAAATCTCAAAGAATCAACATAAACTCATGGAAA1440.11946638348709099No Hit
ATCCGAGGGAGAGGAGGAAGCGGCGCTCATGGGGTTGCAAGGATGTAGAGGA1440.11946638348709099No Hit
CTGTGTCTGGGATTTTAAAGGTCCAGAAAGAAATTCTAATCTGTGTGCCTAT1430.1186367558239862No Hit
GTCAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1430.1186367558239862No Hit
TGACATGGGCTTGTAGATGTTTTTACAAACTCACATTTGAAATCTAAATAAG1430.1186367558239862No Hit
ATAAGTGGGGCGATGGATTCTGGACTCCACCCTCCAAGTAATCTTTGAGGTG1420.11780712816088139No Hit
TCTAGTGGGGACAAATAGGTTCTAATTAAACTAAAGAGCTTCTGCACAGCAA1410.11697750049777658No Hit
AAATTGGGGAAAAGAGGAAGATTTGCAATCAACTAAAGAAGAGAGATTTCCA1410.11697750049777658No Hit
GCACAGGGGGGAAAATAAAGAAGAAATGGTCAAATTCCTAGACACATACAAC1410.11697750049777658No Hit
ACATAGGGGAGCCCCGGATGGTCAGTTAAGAGCTGTTAACCACAGACCTGAG1410.11697750049777658No Hit
GCACTATTTTTTCTCCGCAATACCACTAACTAACGGACTATATATTTAGTAT1410.11697750049777658No Hit
GGGAACGGGTATGTATGCCTATCTAATTATAAGTATCATTCACACTGTCTCC1400.1161478728346718No Hit
CACAAGGGACATCCAGGTGATCTCAGAAAATTGTGCAGTGAATAGGCTACTG1400.1161478728346718No Hit
AATGAGGGGTATTTTAAGATCCTTACATTATGAATGAAGCAGTATATTATTT1390.11531824517156701No Hit
GCTAATGGGATTGAGTCCGGGTGATAGTTTCTCCACATTTGATACTCGTATG1380.1144886175084622No Hit
GATAACGGGCACCTGCCTTTTCACCGATAGAAGCTGGGATAGGATTTGCTTT1370.11365898984535741No Hit
AACAGTGGGTACTACTAAATTGTCTCCACATGGAAAATTTCTTGTGACATGT1360.1128293621822526No Hit
ATTTACGGGATTCCTTCCCACCGTTTTAATGCACATAGTAAGTGGGGAGCTG1350.1119997345191478No Hit
ACACATTCAATGCAATTCCCATCAAAATTCACTATTCTTCACAGAATTAGAA1350.1119997345191478No Hit
GTTATAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1350.1119997345191478No Hit
ATCGTCGGGGACTACAGGATGTGGCTGACAGTGCTCACTGAAAGGAGAGTTG1350.1119997345191478No Hit
ACTGGCGGGATCACAGTGAAAGGCAAAATTATTCACTCAGAAGTAATATAAA1340.111170106856043No Hit
TGGCATGGGGGCACCGGCCGCGCGGCGGGAGGAGGTGCCGGCCGAGCACCGC1340.111170106856043No Hit
GTCATTGGGGAAATCAAAATGGAACAGGCTAAAACAGAGGATGAAAAGTCCA1330.11034047919293823No Hit
GCTCTTGGGCTGATTTAAAAAAACAACAACACAGGATTGAATTAAGTAGAGT1330.11034047919293823No Hit
TATTGGGGGTCCACTAATTTTTTTATTTTGATATTAGTCTTTTTAAAAAAAT1330.11034047919293823No Hit
TTGTCAGGGCTGCAGAAAAGGCATACCACGAGCAGCTGTCGGTGGCAGAGAT1330.11034047919293823No Hit
AGAGATGGGCCTTTTTCCCACCCCCTAGCGCCGCTGGGCCTGCAGGTCTCTG1320.10951085152983342No Hit
CTCCCCGGGGTCTGCCTATGCAAATTTCTATAGCATTTGGGGCCCAACCTAA1320.10951085152983342No Hit
CGTCATGGGATTTCAATGCATTGCATATTTAGCCTTCGGCATATTTTTCATG1320.10951085152983342No Hit
GATAATGGGAAGCCACTGCACTCCAGCCTGGGTTACAGAGCATGACTCCATC1310.10868122386672861No Hit
TCAGGAGGGATATTATAATGTAAAAATGACAAGGCAGGTACTGGGATTTTGC1310.10868122386672861No Hit
TTGCATTGGGCCCACAACAAAGAATTATCCAGTCAAACATGGGTAGTACCAA1310.10868122386672861No Hit
TACCCCAGGGTTGATTTTAATACGGTCATCATAAAATAAGTTAAAGGACATT1310.10868122386672861No Hit
GCCCTGGGGCACAAGTGGCATTGATACTTTTAACATGCGTTGTGGAAATTAG1300.10785159620362382No Hit
GCGCCCGGGTATTCACATGACCTACAGGATGTCCCATCTGCAGGGCTGAGTC1300.10785159620362382No Hit
AGCAAAGGGACTAATAAATCCTGTATATTTAGATATTATGCTAGCCATGTAA1290.10702196854051901No Hit
GTGCTTGGGAATCTTAAGAAATTATCAAGTTGGAACCAAGAAATAGCAAGGA1290.10702196854051901No Hit
AGTCTAGGGACAGGCATGAGCCACTGTGCCTGGCCTAGTATTGGTCATTAGT1290.10702196854051901No Hit
CTCTCAGGGATGGTCCATATATTTTTGTTTTCATTTTAGAACAAAATCCAGT1290.10702196854051901No Hit
TTTTTAGGGAAAGCAGACAAAAATAAAGAAAAATGAATGAAAGGAATGAACA1290.10702196854051901No Hit
GCTTAAGGACAAGATTGAAAAGGCTGTGGTGTCTCAGCGCCTGACAGAATCT1290.10702196854051901No Hit
CTAACTGGGCCCCTGAGGTGCAGGGGAAGACCAAAGTTTGCAACTCGAACTG1290.10702196854051901No Hit
GATTTTGGGAGAGGTAAGTATAATGAATGTTTTATTGTATCTTCTTTGCAGC1280.1061923408774142No Hit
CTATAGCGGGGGAGGCGGGTGGAGAAGGATCGGGGCCCTCGCCGCTCTGTCT1280.1061923408774142No Hit
AGAAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1280.1061923408774142No Hit
ACACAAGGGATTAAAATGGTATAATTGTTTATTAATGAATGCCAAGCTAACA1270.10536271321430941No Hit
CTTCTTGGGTCATCTTGTCCTTCCTCAGTTGTACTCTCATGGGAAACAGCAG1270.10536271321430941No Hit
AAGAGAGGGTTGCAATAACTTTCCAAATTTTCTAAATCCATGAATACCCTTT1270.10536271321430941No Hit
ATACATGGGTGCTGTTGAAATGCCACATTCGTCAGTTGGGGGCTCGCATTTA1270.10536271321430941No Hit
GATCCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1270.10536271321430941No Hit
TTTGTGGGGGGGTTCTCTCTCCTGAGCCTAGATTTGCCAGGGTCTTGGTGCC1270.10536271321430941No Hit
GAGAATGGGGAGTAGGGCCGAGCGCGGAACGCGAGGGGCTGCTGGGGTGTTT1270.10536271321430941No Hit
CTAGTTCTGGGACTGTGTGCTGTATGACATTGTCTCTTCACATCACTAAGTC1270.10536271321430941No Hit
CGCATAGGGGTTGTGGGCATTGTTTTCTACAACCAAATTCTGGGTTTTTTTC1260.10453308555120461No Hit
GCTCTTTTCTTTCTTTCTTTCTTCATCTTTTCCTTTATTGCCCTGACCACCA1260.10453308555120461No Hit
ATAAGCGGGATGTATACACTTTTTGGCTTCTTTCACTCGACATAATTATTTT1260.10453308555120461No Hit
CTTTTTGGGAAACATACTGTGTGTCTGGTTCTACATAATGAAGAGAGTGGAG1260.10453308555120461No Hit
AATCTTGGGTATTCAAGATGAATTTAAAAGTCAACCCTTCACACAGTTTCCC1260.10453308555120461No Hit
TACAGCGGGTCTATGGTAATGGCTAATATACCATGATGTTTATAGGAGCAAA1250.10370345788809983No Hit
CCACACGGGGAGTTCTGGGATGTTAAGTTGAACTGTAGCTCCACGTTTAGAC1240.10287383022499502No Hit
TAGTATGGGAAAGACCTCACAGCATACTTTGTTTTACTACGTTATTAAAAGG1230.10204420256189023No Hit
GCGGCTGGGATGCCAGTTATGTAAGGACTCCAGTGGACCTCAAGCATGGTAA1230.10204420256189023No Hit
ACAACTGGGTATTCAGAATTTTTATCAAGATTGTTTCCTTCGTGAGTCTGTG1230.10204420256189023No Hit
TCAATAGGGATCTCATTGAATAAAGCCCATAAAAATTCAAAATCAATTGAAA1230.10204420256189023No Hit
CTTCAATCGGGGTGGGAATTCCCAAAGGACAGTGTGAGCAAGAAGAAGAAGT1230.10204420256189023No Hit
TGCTCAGGGAGACATTAATTTATTGATACTTGCGTCTGTCTCCTCTAGACTG1230.10204420256189023No Hit
TATCAGGGTCACTCTGTAATTCTCAAAGACTATTTTCAAAATTTGGTCAAAT1220.10121457489878542No Hit
TGTCTCCGGGGGCAAGGTGCTCTAGGAGGTCTTCCTGGCCTCCTTGAACTGT1210.10038494723568063No Hit
AGGCCAGGTTGAGAACTTTGGAAGACAGAGGGATAAGAAAAATAAGTGGAAC1210.10038494723568063No Hit
GCCATGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1210.10038494723568063No Hit
TCGTCAGGGTCCTGGGACCAGTTGCCCAGCAGAGCTCTTGGCCCCGCTGCTG1210.10038494723568063No Hit
TCTGCAGGGGGATTTGCCTGTATCTGTTCCACTCTGTATCTTGGCCTTTCCC1210.10038494723568063No Hit
TGCATTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1210.10038494723568063No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)