FastQCFastQC Report
Sat 31 Dec 2022
SRR5844671.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844671.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172547
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCTCGTATGC9850.5708589543718523No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCTCGTATGCCGTCT9480.5494155215680365RNA PCR Primer, Index 19 (95% over 22bp)
TTTTCTGGGAATGAGAAGTTGCCACTGCAGGGCTAACTCGCCTTCAGAAATA2880.16691104452699845No Hit
CTATGAGGGGACTCCCACACAATAATAATGGGAGACTTTAACACCCCACTGT2570.14894492515082847No Hit
TAGATGGGGCTGGAAAAAGAAGAAGAAAAGAATGGGGCTAGTGTGAAATCTT2490.14430850724730074No Hit
GTCTGCGGGATCTTGTGATTAAATTATAGACCAAAATGGCTTAAAAATAGTA2360.13677432815406818No Hit
CTTAGCAAGGGGAGTGTGACTCAGAAGGTCCTCAGAGCTGGTAAAAAGTGTA2330.13503567144024525No Hit
CAACACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2290.1327174624884814No Hit
GTCGCCGGGATGTGTTTGCATAAGGAATGTTAAAATTCTTTCTACTACAATT2250.13039925353671755No Hit
TGTAGCGGGTTCCAAACTTGCATTATTCATGAACAACCTTCATGACTTATAT2200.12750149234701272No Hit
CATCACGGGATATCACACAAATTCAAATGAAATTATCTTACTACATGTTAGA2160.1251832833952488No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCCTGTCTCT2140.1240241789193669No Hit
AAGAATGGGAAACAAGATAGAGATGTTTTAAAGGCTTCTGGGTTTGATTTCT2130.12344462668142592No Hit
TTTTATGGGGTGGGTTTTTCATTCGATTTTACAAAATAGGGAAAGGCACTTC2130.12344462668142592No Hit
TTAATGGGGGAGAAGTGGCCCTTGCAGGCAAGAGTGCTGGAGGGCGGCAGCG2120.12286507444348496No Hit
TGGCTAGGACGGCAACCTCCTTTGCCAGGAACTATTTATAAACATCCTGCAG2080.12054686549172108No Hit
CTCACTGGCAAGGAAACAAGGCAAATACAGGAGGAAAAAGGTAGGCTGCTGT2080.12054686549172108No Hit
TTCAGTGGGCTTGGTTGATATTTGAGTTTTAAAAGGGTCAAATCTTTCTAAT2060.11938776101583916No Hit
CGTTGCGGGGAGGCTCGGGAGCCGGGAGGCTGCGGGCGGCCGCTGGACCCGA2040.11822865653995723No Hit
GTTATGGGAGAGGGAGTGGTGGAAAGAAACAATAGGATGGGAAAAAATGAAA2040.11822865653995723No Hit
CGCTGAGGGGTAATTATTTCTGTTTCTGTATTATTCTGCCCTTAACCAATTC2030.11764910430201625No Hit
TAACGAGGGTAGCTGTGAACCTGCACTTCTCTAAAAAATAAAATTAGTTAAA2030.11764910430201625No Hit
CACATGGGGAAGTTAGAATTGTGTGGATAAAACTTAGGAACTGGAAATGTAA2020.1170695520640753No Hit
TCTCAAGGGTGCAGGCGGGTTGGTAAACTTCTGCCTACATTTTGGAAAGTGA2020.1170695520640753No Hit
AAGGATAGGGATAACAAGTCTCTGGCAGAGCACCGAACCCCAGATGTGCTCA2020.1170695520640753No Hit
TCTTCGATGGGACTTCAGAATATATCTTAGTTTCCCCTTTCCCAGTTTAACA2010.11648999982613432No Hit
GGTTTGGGGCATTATTTAACATTTAACATTTACAGTGCATCAGGCAGTGTGG2000.11591044758819337No Hit
TGCACTGGGATGGAACGCTTTCATAAACTTCAGGGATCCCGTGTTGCCATGG2000.11591044758819337No Hit
CCTCGGGCAGCAGAAGTATGGGGATCATTTCATAAAGTCTTTTTGAAAAGTT1980.11475134311231144No Hit
ACTCATGGGGCTACTGGTGTTATGGAAAATCCTTATGAAAAGCTAGAAAATA1970.11417179087437046No Hit
ACCATTGGGAGCCATCAAAGCTAAGAATAGCAAAAGCTACCTTCCTGATTCA1970.11417179087437046No Hit
ATGTTTGGGTGATCAGAAGCAGGTAGCCAATGGGAAGAGCACTTCTGAGTGA1950.11301268639848853No Hit
GCAACAGGGGAATGAGGATGGCCTGACGCCTGAGCAGCGAGTAGACAAGATT1940.11243313416054755No Hit
AGCTTCGGGGAATTAAAATTGTCCTGGAAGACATCTTTACTTTATGGAGACA1940.11243313416054755No Hit
TCTAAAGGTGGGGGGCCCAACGCGTTGACCTCGCGGTCAGGTTGCTTCCGCG1930.11185358192260661No Hit
GCTGACGGGGGTCTACGCCGAGTGACAGAGACGCTCAGGCTGTGTTCTCAGG1920.11127402968466563No Hit
TCACTCGGGGACGCTCTTCCGATCTTCTTTCGGGCAGTGATGTGTGAGCTAT1920.11127402968466563No Hit
CTTGCCGGGGACGGATAATGAAAACGCTGCCACCCCAGAGAGGGTAAACCCA1910.11069447744672466No Hit
TCGCTGGGGTGGAAACTAAGACATTGGACTGAAGGAACTGGCCTGAGGGGGG1910.11069447744672466No Hit
GCTGCAGGGGGAAAATGAAAAAGCATCATAAAATACCATGATTTTGTGGGCT1900.1101149252087837No Hit
AAGGCTGGGGGCTAATTTTTTTATATTTTTAGTAGAGATGGGGTTTCACCGT1900.1101149252087837No Hit
ATTGCAGGGCCTATATGAAATTTACCAAATTAGACCAACAGTTAAGTGTAAT1890.10953537297084272No Hit
CCGTGAGAGCCTCAACGTGGACGTGGTCAAGTACGAGAGTGGCCCTGACGGA1890.10953537297084272No Hit
CGAATGGGGGGGGGCCAGCCGCCGCCATGATCCTGCTGGAGGTGAACAACCG1890.10953537297084272No Hit
CAGTCCGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCC1880.10895582073290178No Hit
TGAAAGGGGAGACCAAGAGGAAGAGGCAGGTCCCAGCTGTGACAAGCATGTC1880.10895582073290178No Hit
GCTTTCTCCCCACTGGCTGGGCTGCATCTGGCCCTGGCTGGAGGCCTTGCTT1880.10895582073290178No Hit
TACGCTGGGAGAACTAATCATATTTTATATCTTCTTCGAAACCACACTTATC1880.10895582073290178No Hit
GATTGAGGGAATGAAAGATCAGGGAAAATATCTGCAATGTATTTTCCAGATA1880.10895582073290178No Hit
GTAAACGGGCACAAGCATTCTTATACACCAATAACAGACAGAGAGCCAAATC1880.10895582073290178No Hit
TCGTCGGGGCGTCTTTCTGGGCTAGGGATGGGGAACAGAAAGGGAGTATGGA1870.10837626849496079No Hit
AGTTACGGGAGCCGCCCGTGACTCTCACACTGTCTCCTGCATGACGGGTGGC1870.10837626849496079No Hit
TGTCGAGGGATCAAAAAGTCTGAAAGACCACAAATAGACAATCTAAGGTCAT1870.10837626849496079No Hit
AAAAAAGGGGAATAATATCTTAAATTATTAAAAGAAAACAATGTCTAGCTTC1860.10779671625701982No Hit
CAAAACGGGGTCTGACATTTTAGCCAGTCAACTTGGAGCACTGAATATATTT1860.10779671625701982No Hit
TACACGGGGGCCTTATGAAGGTTGTATTTGCAGCATCAGGTAGAGTTCAGCT1860.10779671625701982No Hit
TTCATGCGGGGATTCAACCAGCTCATTTTGGGGAGGAGAGAAAATATTCTTA1860.10779671625701982No Hit
CGTACCAGGGAGAAACAAATGAGAGCAAAATGTTAGGTCCTTTGACAAGCAT1850.10721716401907887No Hit
AGCTCTGGGATTGCCTGTTGGTCCTGCAGGCGGAAACCAAATGTTGGAGCAG1850.10721716401907887No Hit
ATTATAGGGATAATTTTCACATTTAGGAGGCAATAGCAAATCTGGGAAGCAG1840.10663761178113788No Hit
TCTTCTTGGGTCAATGGGCAATGAACTCAGTAAGCACTCCTTCATGTTCACC1840.10663761178113788No Hit
CATTTAGGGTGGAAAATGCCACATGGGATAGATGACGGATTAGAATGTATAC1840.10663761178113788No Hit
CAAAATGGGATTCTCACTGGGCGAGAGAAATGGATAGGGAATGATAATGATG1830.10605805954319693No Hit
TAAGATGGGGTGGCAAGCTATACCGGAAAAGCGGCATGTCAAAAATACTTTA1830.10605805954319693No Hit
GCTGCTGGGATCATGGTGAATTAATATTTTTTGGCTGTTTGGTGTATAACAG1830.10605805954319693No Hit
GTTGTCGGGCTATGGGACCAGTAAATTACAGATTTTTTAGGTTTTAAATCAG1830.10605805954319693No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCCTGTCTCTTATAC1830.10605805954319693No Hit
ATACGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1820.10547850730525596No Hit
CTCATGGGAAATGCTCAAAAATAAATGTAACTGAGAAGAGTTAATAATTGTG1820.10547850730525596No Hit
TACGCTGGGTATAGTCACTCTGTTGTGCTATCAAATACTAGTCTTATTCATT1820.10547850730525596No Hit
ATAACAGGGGAAGTGACAGGGAGGGCCGGAGTCAGCAGGTGCCAGACTTTCT1820.10547850730525596No Hit
GTCTTGGGGGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTA1810.10489895506731499No Hit
TATCTTGGGCCGCCAACAGAATTCATACGAGAAGGCGACGATGACCGGACTG1810.10489895506731499No Hit
TTGCTGCGGGCTCCAGAGAAGTTTGGTTTCTTTGCTGCAAGAGGAATGAGGC1810.10489895506731499No Hit
TTTCTAGGGGTTTGCCAACAACCCCAGCCAGAAGTCCAGCCAGGCCCTGCTC1810.10489895506731499No Hit
TTCCAGGGGTTGGATAGTGCTGCTATGATAACAGTACATACTCCAAGGAGAG1800.10431940282937403No Hit
TCATCTGGGGGCTTATAAGTGAGAACATGCGTTATTTGGTTTTCTGTTCCTG1790.10373985059143305No Hit
TTTCTGGGGATATCAGTTTTGGTATCTTCCTCTCTAGAGCATCATTTGAACA1790.10373985059143305No Hit
TATGGTGGGCGAGAAGGGATTTCTTAAATATTTTTAACTTCTAATTTGTATT1780.1031602983534921No Hit
CACTGGGGGACAAAATGTGTGCTTTAACTGTTTACAAGTAAAACCTAAAGTT1780.1031602983534921No Hit
TCAATAGGGACCTATGTCCACTGAAAGTTTAAAGCATAGCAACAATTCTACG1780.1031602983534921No Hit
ACAAGAGGGCATTGTCTTCAGTGGAAATTTTAAAATAGTAATTACTCCTTTC1780.1031602983534921No Hit
AGATAGGGGACAACAGTTGCTTTCATAAAGACTAGTTCTTATCAACCTTGAA1780.1031602983534921No Hit
AAAGCAGGGCTGATGCATCCATACTCAGGGTGTAGGGAGAATACTTTGCATT1770.10258074611555112No Hit
CATTAAGGGGAAGTATGATTATATTCAGAAACGACTAGCTTTGTAAGTGATA1770.10258074611555112No Hit
CACCTGGGGCATAGTTTGACATTTTTCTGGCACATTAAATTTAAGAGAATTT1770.10258074611555112No Hit
AACACGGGGACGAGATGATGATGCAGTGTGTGTCCCGCATGTTGGCCCACCC1770.10258074611555112No Hit
GCCACAGGGGGAAACTTTCTTACTCAAGAATGACGGCAGTATTGTTTTCTTA1770.10258074611555112No Hit
ATACGCGGGCATGGAACTGCCCAAACTAAAGCTTTCGGGAGTGGTCAGACTG1760.10200119387761014No Hit
CGCTTGGGGTTCTCACCTATCAGTAAAAACCTTGAATGCAAATGGATTAAAT1760.10200119387761014No Hit
GGCGAGGGACTTTTGTTTTGTGGGGATAACGAGACAGAAGTGAAAGGAAAAC1750.1014216416396692No Hit
TTGTCTGGGGTTTCATTTACTTTCCAAATAGCAAATAAACCAAGTTCTACTG1750.1014216416396692No Hit
TGCGATGGGGGCGGGTGCAGGTGGCGCGCTATGGCCGCCGGGACCTGCCCCG1750.1014216416396692No Hit
TAACTAGGGGGGATGTAAAGGTAAAGAAAGAGTCAAAGATAATTCTTGTTTC1750.1014216416396692No Hit
CTCATAGGGACTGCAGGTTGTGGGTCAAAGTTCTATTTATATATATATTCTG1750.1014216416396692No Hit
GACGTAGGGACTAAGGGTGCCTTTTAGGAGAAATAAAGAGGAAGATTTGCAA1740.10084208940172822No Hit
GCGAATGGGATGAAGGGAGTCATGGGGAATTCCTGAAAATTTTCTTCCTTCT1740.10084208940172822No Hit
AAAAAGGGGGTATTTAGTCTTTCCATAAAATTTAAAAAGCCAAGAATGAACT1740.10084208940172822No Hit
GGTAGATGGGGTGTACAATTTAAAAATAATTTTTAGCTAGGTGTGCTGGTGC1730.10026253716378726No Hit
AGTAATGGGCAACCATTGAAGGAGGACAGTACCTCGTATCCACTCAATCCTG1730.10026253716378726No Hit
GCGTTGGGGATTTGATTAAAACATATGCAAATGTTTTTCTTTTACTATTCCC1730.10026253716378726No Hit
ATACTTGGGTGATGGAAACTCATGGACTTTGTTAAGGGAGGCATTGAAAGCA1730.10026253716378726No Hit
TTTATTGGGCGACCTTTACATCTATTTTATTTGCAGGTCATATATCTAATAA1730.10026253716378726No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)