FastQCFastQC Report
Sat 31 Dec 2022
SRR5844520.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844520.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37440
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT16574.425747863247864No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC14883.974358974358974No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA3100.827991452991453No Hit
ACGCGCCTGGCTCTGCCACTGTTCTCTTCCCTCTGCTGCAAAGCCCAGTTAA2560.6837606837606838No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT2280.608974358974359No Hit
GTAATTGGGATAAACAATGGTCACGTTGTGATAGGGATAACAATAAATCTAT2070.5528846153846154No Hit
TCAGCTGGGAGTGTCATCTATTGCGTGATTTTTATATAAATGGAATCATATG1980.5288461538461539No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA1960.5235042735042734No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC1960.5235042735042734No Hit
TCCGTGGGGAAGGAGAACTTCAGTTGCTTGACTCGTTTGGATCACAACCGAG1800.4807692307692308No Hit
GACTGAGAGTGCCCCCACCCATCTCATGCCCCAGGGACCGCCACTGCCCACC1800.4807692307692308No Hit
CTACCGAGGGGGAATAATATTCAGCAATAAAAAGGAACAAAGTATTCATATA1660.44337606837606836No Hit
AGCATGAAGGGATAATAAATGAAAAAATAGTCTTACTGATTGCAAATTGATA1630.43536324786324787No Hit
TGGTGGGCTAGGCTCACTAAACATTCTACTACTCACTCTCACTGCCCAAGAA1580.422008547008547No Hit
AGCGCTGGGAATGCTTTGCTGGTGCTCTGGCATTTTGTCTTTATCTCATCAC1580.422008547008547No Hit
TGTGACGGGAGTACTATTCAGCCATAAAAAAGAATGAGATCCTGTCATTTGC1440.38461538461538464No Hit
ACAGTAGGGATCTAGTGTTGGGATGTAATAAAGTCAGACAAAATGCATACTT1360.36324786324786323No Hit
AAATATGGGAGGATGGTAGTGGCACCCTCAGAGCCCCATCTTCAGCATGTTC1360.36324786324786323No Hit
CTTCCTGGGCGAACTGCTGGGATTACAGGTGTGAACCATTGTGCCCAACCCT1330.35523504273504275No Hit
GATCTTGGGCACGGGGAATGAGTGAAATAACCAGCAAGCAAACACCTGTCAG1290.34455128205128205No Hit
GATATAGGGGATAACTCTATTATATGAATTCTTGTGAGTTATTTTATTGTAG1280.3418803418803419No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAACTCGTATGC1260.33653846153846156No Hit
TAATCTGGGGGTATAATTGGGATTGAAAACTAAAACTTGGAACTAAGATAGA1250.33386752136752135No Hit
GTTTCCGGGAGACTAATAAAGAAGAAAGACAGAAGAATCAAATAGACACAAT1230.328525641025641No Hit
CATATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1190.3178418803418803No Hit
CACGCTGGGACCAGACTGCCAGACGACTACATCATTCTGCCCACAGACCTGC1100.2938034188034188No Hit
CATAACGGGGGATCCCCAAAGGATGTCTATTTTTGGCCACTCCATGGGAGGT1100.2938034188034188No Hit
AACTTTGGGTTATACCACATATCGCATATGACCATCTTTCCATCAAATCAAT1090.29113247863247865No Hit
ATAACGGGGGAAGCAAAGGTTTATTTTTTGGTGTCCAGATTATCCATAGCTC1060.2831196581196581No Hit
GCTTAGGGGATGTCTCTTTTAAGCAAATGTTTTGATGCATATCTTTTTAATT1050.28044871794871795No Hit
TAACTGGGGACTGGAGCCCATACCTAGGAAAGGAGGCAAGGATGTATTCCAG1040.2777777777777778No Hit
GTAAATGGGCTTTGCTGGGCCTCCGCAAGGGCCTCTCTTGGGTCTGTGTCCT990.2644230769230769No Hit
CAGTTAGGGGCGTGTTTAATGATGATGACTCCGCAAGCCCTCTGACATTGTG970.25908119658119655No Hit
TTCGATGGGTGCTGGGACAGAGTCCTGTAATCAGTGCATGTCCTTAGTCTGT960.2564102564102564No Hit
TGAATAGGGGAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCAT920.24572649572649571No Hit
AACAATGGGATTTAAGGATAAAGGTAAATCATTCAAGGCAGTTACCAACCAC920.24572649572649571No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGT920.24572649572649571No Hit
TACGGAGGGTGGCCATGAAGGACCTTTTGACCAACTCCCTGTCATTCCTAAC910.24305555555555555No Hit
CTTTCGGAGGGGAGACAAAGATGGCCTCATGTACTGGTACCAATTGGATCAA910.24305555555555555No Hit
ATGCCAGGGGCGGCGTCGCCAGGAGGAGCGCGCGGGCACAGGGTGCCGCTGA910.24305555555555555No Hit
GTAATAGGGTGTGTTTTTTGGTAAAATGGTGATTGTAGATAAGATTAGTTCC900.2403846153846154No Hit
ACACCGGGGAGGTCAGCCATATCCAAAAGACCACAAGTCATTACTAAGTTGA900.2403846153846154No Hit
ATTATAGGGACTGTCATAGAAAAACTGAATTCTCGTAAGATATTGTTGTTTC880.23504273504273504No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAT820.21901709401709402No Hit
AGGCACGGGAGAAATAGCTTGCTATACTGAAGGATGGAGGTAAGGACTGGTA810.21634615384615385No Hit
TTTATAGGGTACATCTATTGGACAAGTTTTAATTAATTCTTTCCCTTGGCTG740.19764957264957264No Hit
CTCTTTTTCTCAGTAAATCATATCATCTGAAATATTACAAATTTCAAATTTC740.19764957264957264No Hit
ATGCTCGGGCAAGATCACATCACATTTGTAAAAATGGTAAGTTAATGCATTT730.19497863247863248No Hit
GACATGGGGACTGAAACATGCTTTAAATCGTGTCTAAAATCTGATTTGAGCA720.19230769230769232No Hit
GTTTCAGGGAATGTTGCGTCATTAAAGGTCAAGGGGTCATGCTATTAATCTC720.19230769230769232No Hit
TTTACTTGGGGGAGTCAGTCATCCAATTTTTCATGACAGAAAAACAACATTT710.18963675213675213No Hit
AGCAATGGGAAGCTCGGGGCCATGCCGTGCCACCGTCACCAGAGCCAGCTGC710.18963675213675213No Hit
ATTTCAGGGGTGTAAACCTCAGGGTTAAAACAACCCTGTTATATAGGGATAA710.18963675213675213No Hit
TGTAGTGGGATTGAATGTTTAATATATGATAAAAATCAGTACTAGTATTATA690.18429487179487178No Hit
TCAATGCGGGATCATTTAATAATATACTTGTGGACTAAAAGATATAAGTGCT680.18162393162393162No Hit
GTAGTGGGGACCTCCCACAAGGATTACCCCTTCCATGAAGAGTTTTGAAGCT680.18162393162393162No Hit
GAGAATGGGGCAGCCTTCCTCTTCTGGGACTGCTGTGAGGCAGAGGAATGAT660.1762820512820513No Hit
GCTAGGGAAACTGGAACAGTTTACTACCATGAATCTATCCTATGTCTTCATT650.1736111111111111No Hit
ATAAATGGGGGGAAGAAGGAAGGAAGAGTAAATATTAACAAACATGATTTTT640.17094017094017094No Hit
ATTATAGGGCAGAGACTTGACTGCCAGTTGAGTAAAGGTGCAAGATATGAGG620.1655982905982906No Hit
ACTTTTGGGAGGATGGCAATTGCTCAGACTTGTCCTTTGCTTGTCTGGTTAT620.1655982905982906No Hit
AGAACAGGGGAGAGGAGTTCCAGCTGTTCTGGCCACACTGGGCTGGTGGGGA610.16292735042735043No Hit
GCAGTTGGGAGAGACCAACCGAACCCCCTTCGACCTTGCCGAAGGGGAGTCC600.16025641025641024No Hit
ACTAAAGGGGATTTTTAGAACCTTTGCCTATATTAGGTTGTACTTATGTACA590.15758547008547008No Hit
TCAATTGGGGATTAAAATAATTTGAAGGCGATCTTTTAAAAAGAGATTAAAC590.15758547008547008No Hit
GTTAATGGGATACAAACTGATGTTGACTTTTGTGAGATATATCTTCTGTATC590.15758547008547008No Hit
AACTTCGGGTGAGGGACATGGGGGTACAAAGTAGGCTTTTTAACTTTATAAA590.15758547008547008No Hit
TTCCAAGGGACAGTCCTTCAGCAGATTGATTCAGTGGCTAATGCTGATATCA590.15758547008547008No Hit
TGAATGGGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTC590.15758547008547008No Hit
TCTGCCTGGGAGGGGCTTTGGGGACATGGCCTGTCCCCACATCCAGCACTTG570.15224358974358976No Hit
TATAACGGGATAATGATAAAGAGTTCAATTCAACAAGAAGACTTAACTATCC560.1495726495726496No Hit
AGTTCTGGGACATTTTCAAGTTTATTAGGTTAAGAAAAACAGGGCAGCCTTG550.1469017094017094No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAACCTCGTATGCCGTCT540.14423076923076925No Hit
CTATCAGGGCAACTCCTTGAGGGTAAAAGGGAACTCCTCCTTTTTGAAAGCT520.1388888888888889No Hit
ACGTTTGGGGTAACTAAAGTTACTGGCTTGGTCTTATTTGCACAGTTTTTGC510.1362179487179487No Hit
CTTAATGGGAATTCTATATTTTCTGGCAAGTTTCTATACGAAGTATGATCCA510.1362179487179487No Hit
ATAGCAGGGAACAGTCGTATGATTTCTAGCCCATGGGTTTAAAACTGTATCT510.1362179487179487No Hit
GCCACTGGGGCACCATCACAACAAAGGAACTTGGAACTGTCATGAGGTCACT500.13354700854700854No Hit
TTTGGAGGGAATCAGAGATGGCACAGATAATAAAAACAGGAATGGATATGGA490.13087606837606836No Hit
GTTTTGGGAAAGACTACAGTCATACCTCAACTATCTACAGAGGACTGGTTCC490.13087606837606836No Hit
TACTTGGGACAGAAGAGAACTCCTAGAAGAGCTTTACTTAACTATGAAAATT490.13087606837606836No Hit
GCGTAAGGGCAGAAAAGTAGTGAACTTATTTTCAGTAGACATAGAAAACAAT490.13087606837606836No Hit
AAGCAAGGGGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAA480.1282051282051282No Hit
TCAATGGGGACTGGTGGGTGCTCTGAAAACCCACAGGATCCCACCTCCAGGC480.1282051282051282No Hit
ACTAATAGGCTCTCTGTTGATATTTAAAGAAGTGAGGGCTGTGGGTATTCAT480.1282051282051282No Hit
TAAGCCGGGAGCCCTCACAGATGCCCAGCCTCATAGCTGAAGTTGCCTGAAT470.12553418803418803No Hit
GATTTTGGGCTGCCACTCTTAACACTAAGTGAGAGTTGGGGAGAAACTGAAG470.12553418803418803No Hit
TATCCGGGGGCGCATGCTCCGTTGTCCCTTCAGACTTCACTTATAACACACA460.12286324786324786No Hit
TACTAGTGGGAGCCCACCAGGGTCCAGGGAGATGTTCGTTCTCGCTTTAAGT460.12286324786324786No Hit
GTTATGGGGAGCCTTGGGAAGCATTTCCATCTATCGGGACACCCACCAGTCG450.1201923076923077No Hit
TTCATGGGGCAAGAAGTATAATATGCGTGTGGAAGACTACGAACCTTACCCG450.1201923076923077No Hit
AGTACTGGGCAACCTTTCTCCCTTCTCAAAATGCTTGCCTCATAATGCATAA450.1201923076923077No Hit
GTCGACGGGATGCACTTGGAGTCCATGAGCCAATGGGATATGCAAAGACGCT440.11752136752136752No Hit
CAGTTAGGGGGCAGAGGACGATAAGCACTGCTTCCCGCAGGCATTTTAAAAA430.11485042735042736No Hit
TAGTGTGGGGTCCGCCAGACCGCCGCCGCGCCGCCATCATGGACACCAGCCG430.11485042735042736No Hit
CAGCATTTTGGGAGACTGAGGCGGGCTGATCACTTGAGGTCAGGAGATCGAG430.11485042735042736No Hit
AACTTTGGGAGGCCGAGGTGGGCAGATCACTTGAGGTTGGGAGTTCGAGACC430.11485042735042736No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCC420.11217948717948717No Hit
GTTATAAGGATACCAATTAAACATTTTCATAGTTCTGCCTATTGTCCTTCCC420.11217948717948717No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCTGTCTC420.11217948717948717No Hit
ATTGTTGGGGGGCTTATGGAGGACCCTATGAAAGTGTATAAAGATAGGCAGT420.11217948717948717No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCTGTCTCTTATA410.10950854700854701No Hit
GATGCCGGGTCTCTCTGATTGTATGTGGGAGACACCCATTGCTCAGGCCAAA410.10950854700854701No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGA410.10950854700854701No Hit
CATTTTGGGGATTTGTATGATCTTATAAAATGAAATAAGCAGACTTAAAAAA410.10950854700854701No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAACCTCGTATGC410.10950854700854701No Hit
GGCTATGGGGGGTGCGGAATCAAATAGGAAGCATGCAGAGACTCGGCAGCTT400.10683760683760685No Hit
TTGCTTGGAGTGTCTTTACAAAGATAATAACAGTTGTGTTCTTTGCTCTCGT400.10683760683760685No Hit
GGTAGTGGGATCAACGGGATATCAAAATGATTTTGAGGATAGTAATTTGAGG400.10683760683760685No Hit
GTGCTTGGGGTAAACCAAAAGATGGAAGACAAAAAGACAAACACACCCACAC390.10416666666666667No Hit
CAAGTGGGATAGTGAACTCCTAAAACTCAAAAACAAAATAAACAACCCAATT380.10149572649572651No Hit
GTTAGAGGGCTGAAGCGAGGGATATAAATTACAAAAGAGATTTTAAATTAAT380.10149572649572651No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)