FastQCFastQC Report
Sat 31 Dec 2022
SRR5844076.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844076.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131195
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTCGTATGC6230.47486565799001484No Hit
CTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTCGTATGCCGTCT6150.4687678646289874No Hit
CATAGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1690.12881588475170547No Hit
TCACAAGGGTCCGATCTCTGGAAGGGAGTAGCTGGAATTACAGGTGTGCACC1500.11433362551926522No Hit
ACTTCCGGGGTATTATGATGGGATAAAAATGGAAGTTTCCTTCTGAAGTCTT1500.11433362551926522No Hit
AGCTACAGGGGAAGATGGGAAATGTAGTTTCTGGCTAAATATCAGTGTATAA1490.11357140134913679No Hit
TCCTTGAGGTTATTGGGTTCTATGGCATCTATTATATCAAATTCCTTGCCCT1480.11280917717900836No Hit
CTAAAGGGGCCATGGAAGAATGGAGATAGATTCTTTACAGATCAAGAGATTG1470.1120469530088799No Hit
CACGTCGGGAAGACTGTGTGTGGAGAAATTATATTATTTTATTTTATTATTG1470.1120469530088799No Hit
AAAACTGGGGGCGGATCTAGATCCATTAGAAGGTGGTGACATCCATTTGGTC1440.10976028049849461No Hit
TTTAATGGGAGGATAAGAACCTCACTTCCTTACTCCTCCAAAAAGAAGTGGG1430.10899805632836616No Hit
GCTGATGGGATGAACCAGAAATCCTTACATTTATTTTCGGTTGATTTTGACA1410.1074736079881093No Hit
GAAATAGGGGAGGATTCAATTCCACCGAGATATAATTACCATATGAGGAAGT1410.1074736079881093No Hit
ATGAAAGGGAGGCTGAGGCAGAGAGGATTTGTGAAATTAGAGAGACGGTGTG1410.1074736079881093No Hit
GAACAAGGGGGAAAACAAGACTTTTCATTTTCAGTTTAATTCCTCAGGGAAC1410.1074736079881093No Hit
AGATCGGGGAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCC1400.10671138381798087No Hit
TCACGCGGGTAGTGAGACACCAACTGGGGCAGCCATGGGAGTGCTAGCATCA1400.10671138381798087No Hit
CACTCTGGAGGGCGGCCCCAGAGTTCCGGGCAGTGGGGGCGGCGTTGAGGGA1350.1029002629673387No Hit
TAAGCAGGGAACTTCCTGTGGCCCCCACAAGCTGAGTCTGGCCTTCTCCAGC1350.1029002629673387No Hit
TAGCACGGGAATCCAGCAGTCTTCGAAGGCACAGTGATTATTGCCATTTTAC1350.1029002629673387No Hit
AGGTCTGGGGTGAGGATTGAGTAAGATAATGAGTAAGATTCTAGTAGAGAGA1330.10137581462708181No Hit
GAACAGGGGTCCTGATTCAAGAGAATAGTAATATTTTCATAAGCAGAAATTT1330.10137581462708181No Hit
GTTTGCGGGTGACCAGCGGAATTAAGTTATAAATCAATAACAAAAAGATAAT1330.10137581462708181No Hit
TTACTTGGGGAACTGAAGATACAAATTCAATTGGTGGGCTGCAGCGTATTTT1330.10137581462708181No Hit
TGTACAGGGACAGTGAAAGAGTAAATTAGAAAGTGAATGGATGACTGTTCAT1330.10137581462708181No Hit
TCAGCAGGGTCAACTACTTCCCAATAGGGAAAATTTTTAAAAAAAGTTATTT1330.10137581462708181No Hit
GTGCGGGTGGGTCAGATTTCCCAGTTCTTTGCAATCCTAATAAATTCTGTTT1330.10137581462708181No Hit
CGTCATGGGATCATTTTTGAGAGTGAAAGACCTGAAATACTAAGGAACTGAG1330.10137581462708181No Hit
GTGAAGGGCAAGTAGCTGGGACTTCAGACGCCCGCCATCATGCCCGTTTAAT1330.10137581462708181No Hit
AAGAATGGGGAACACGGATAGATTCAAGGCATTTAGTAGTAATCTAGTTCTT1320.1006135904569534No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)