##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841250.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2980703
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.59243138279795	39.0	37.0	39.0	31.0	39.0
2	37.20281222248577	39.0	37.0	39.0	33.0	39.0
3	37.39399497366896	39.0	37.0	39.0	33.0	40.0
4	37.84697636765555	39.0	37.0	40.0	33.0	41.0
5	38.30227567120911	40.0	37.0	41.0	34.0	41.0
6	38.225317651574144	40.0	37.0	41.0	33.0	41.0
7	38.17617756616476	40.0	37.0	41.0	33.0	41.0
8	38.08387148937683	40.0	37.0	41.0	33.0	41.0
9	37.968064580738165	39.0	37.0	41.0	33.0	41.0
10	37.889642476959295	39.0	37.0	41.0	33.0	41.0
11	37.57403270302341	39.0	36.0	41.0	32.0	41.0
12	37.84319001255744	39.0	36.0	41.0	33.0	41.0
13	37.93057275414558	39.0	36.0	41.0	33.0	41.0
14	37.953451249587765	40.0	36.0	41.0	33.0	41.0
15	37.88420147864447	40.0	36.0	41.0	33.0	41.0
16	37.934437949705156	40.0	36.0	41.0	33.0	41.0
17	37.82913527446377	40.0	36.0	41.0	33.0	41.0
18	37.8896226829711	40.0	36.0	41.0	33.0	41.0
19	37.83763930857922	40.0	36.0	41.0	33.0	41.0
20	37.67168885997699	39.0	35.0	41.0	33.0	41.0
21	37.63634585532339	39.0	35.0	41.0	33.0	41.0
22	37.54362242732671	39.0	35.0	41.0	32.0	41.0
23	37.43373559861549	39.0	35.0	41.0	32.0	41.0
24	37.16638759379918	39.0	35.0	41.0	31.0	41.0
25	37.176017970619284	39.0	35.0	41.0	32.0	41.0
26	37.136054611691804	39.0	35.0	41.0	32.0	41.0
27	37.07157530206424	39.0	35.0	41.0	32.0	41.0
28	37.04358971081488	38.0	35.0	41.0	32.0	41.0
29	36.94861242546483	38.0	35.0	41.0	32.0	41.0
30	36.88646586921587	38.0	35.0	40.0	31.0	41.0
31	36.687830820693506	38.0	35.0	40.0	31.0	41.0
32	36.63357190926245	38.0	35.0	40.0	31.0	41.0
33	36.49591761415141	38.0	35.0	40.0	31.0	41.0
34	36.427094582433405	37.0	34.0	40.0	31.0	41.0
35	36.399459064097464	37.0	34.0	40.0	31.0	41.0
36	36.32135739809037	37.0	34.0	40.0	31.0	41.0
37	36.23752893257862	37.0	34.0	40.0	31.0	41.0
38	36.084048917379185	37.0	34.0	40.0	31.0	41.0
39	36.03262963408699	36.0	34.0	40.0	31.0	41.0
40	36.015276355596704	36.0	34.0	40.0	31.0	41.0
41	35.86950031944157	36.0	34.0	40.0	31.0	41.0
42	35.64370804758213	36.0	34.0	40.0	30.0	41.0
43	35.59247027481088	36.0	34.0	40.0	30.0	41.0
44	35.54825954837997	35.0	34.0	39.0	30.0	41.0
45	35.348478802571634	35.0	34.0	39.0	30.0	41.0
46	35.12993016658626	35.0	33.0	39.0	29.0	41.0
47	35.149092074074694	35.0	34.0	39.0	30.0	40.0
48	35.10209467391756	35.0	33.0	39.0	29.0	40.0
49	35.42286888864731	36.0	34.0	39.0	30.0	41.0
50	35.701303763395956	36.0	34.0	39.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	92.0
14	527.0
15	3598.0
16	948.0
17	5.0
18	1.0
19	1.0
20	6.0
21	16.0
22	68.0
23	197.0
24	510.0
25	1190.0
26	2879.0
27	6482.0
28	13363.0
29	26099.0
30	47185.0
31	78741.0
32	124118.0
33	184551.0
34	273607.0
35	278590.0
36	320052.0
37	367664.0
38	392300.0
39	453042.0
40	404871.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	39.004422782142335	20.656268001206428	40.339309216651245
2	27.459528842692478	20.61463352772819	27.692896608618838	24.232941020960492
3	29.089245053935265	20.142295290741817	24.158596143258823	26.609863512064102
4	24.785059095119507	21.86662005573853	28.58017051682103	24.768150332320932
5	23.332247459743556	24.82468062064553	24.81203259767914	27.03103932193177
6	22.299504512861564	24.035504375981105	23.890739869084577	29.774251242072758
7	28.082234291709035	21.03091116424548	21.659588358853597	29.22726618519188
8	24.321779123918084	22.276221414880986	25.86101332470897	27.540986136491963
9	26.017855519318765	26.31869059077674	25.268669840638264	22.39478404926623
10	34.326767879926315	20.130653741751527	21.984075568750054	23.558502809572104
11	24.95538804100912	19.326380387445514	26.163995540649303	29.55423603089607
12	26.962129403701073	22.31520550688881	22.865042240035322	27.857622849374795
13	27.359854369925486	21.700551849681098	23.074590121860513	27.865003658532906
14	22.53897822090963	21.360095252697096	21.506939805810912	34.59398672058236
15	23.764058344625415	27.758082573137948	25.87892856148365	22.59893052075299
16	22.85323294538235	28.495257662370253	20.101432447311925	28.55007694493547
17	22.46859214084731	33.17579778998444	19.422062513440622	24.933547555727625
18	23.274878443105536	23.11625814447129	19.285819486208457	34.323043926214716
19	28.776701335221926	21.359826859636804	20.768288554746984	29.095183250394285
20	25.101561611472196	20.036581974118185	23.226165102662023	31.6356913117476
21	23.953946434784008	28.509046355842898	20.780097849399954	26.756909359973136
22	27.004636154625267	22.335435633808533	23.88131256284172	26.77861564872448
23	29.813369530610736	21.247470814770878	25.083310883372146	23.855848771246247
24	25.286887019605775	21.734738415736153	26.945824525288163	26.032550039369905
25	25.572135805803203	27.22240567942954	20.999443319102536	26.206015195664715
26	24.99863811242382	30.236117258520306	22.903374076469987	21.861870552585884
27	24.379684602131185	26.091484861121785	23.082811145374933	26.4460193913721
28	24.006140982108032	21.82197439345883	20.86469146021403	33.30719316421911
29	24.295384546821282	23.161210444916566	25.464201879160235	27.079203129101913
30	24.482200130706254	23.92608466720245	24.361666936194233	27.230048265897068
31	24.32767756534361	29.392863786933614	22.82875875616017	23.45069989156261
32	27.670551950981437	24.389238369913826	21.830571266252235	26.109638412852497
33	23.341613932701964	21.30732750310073	26.072521072003084	29.278537492194225
34	24.80865643651734	27.18609930560394	26.336755962748786	21.668488295129936
35	21.971130326987414	22.728857708318316	22.070653166198383	33.22935879849589
36	21.898441944216334	24.791362776802625	28.04865631799478	25.261538960986258
37	22.06086320148242	29.748274468083522	21.13967372166208	27.051188608771977
38	26.62092785343557	25.95102630763888	22.53210320328118	24.895942635644364
39	26.78920282993519	27.20065911724612	24.811699709573627	21.198438343245073
40	26.82848171871309	23.19500891728205	24.82511784437253	25.151391519632334
41	26.62648519150513	27.801660480013435	22.292978177461638	23.278876151019805
42	21.799544303817186	32.050800272124846	21.62531898148671	24.52433644257126
43	25.681988696437386	23.342510228551138	22.253839692210555	28.721661382800917
44	26.54501694230872	21.970315390038735	21.533145800827125	29.951521866825427
45	22.602191733041114	22.622645550611544	29.34596406798704	25.429198648360302
46	20.148169425100832	27.148614195246317	30.168509373520585	22.534707006132262
47	22.988952451148727	31.083103699546875	22.920899885252904	23.007043964051494
48	26.16112985173527	27.75316872843022	21.317069243583582	24.768632176250925
49	21.614947896676348	28.354324786628183	22.088115295104263	27.942612021591206
50	20.551061931823877	29.525613655270572	28.30448393487754	21.618840478028016
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4183.0
1	3307.5
2	2182.0
3	1799.0
4	1638.0
5	1736.5
6	1822.0
7	1411.0
8	1052.0
9	1594.0
10	2377.5
11	2597.0
12	2648.5
13	3045.5
14	3790.0
15	4888.0
16	5693.0
17	7054.0
18	8392.0
19	11417.0
20	14102.5
21	11644.5
22	10431.0
23	16610.0
24	23010.0
25	23528.5
26	24715.0
27	30307.0
28	35894.5
29	42723.5
30	48670.0
31	59152.5
32	72423.5
33	70162.0
34	72282.0
35	82756.0
36	84091.5
37	88185.5
38	95771.0
39	105412.5
40	110026.5
41	109323.5
42	116366.5
43	122722.5
44	127612.5
45	139268.0
46	152201.0
47	151345.5
48	155857.0
49	195094.0
50	260923.0
51	277042.0
52	220850.5
53	179362.0
54	174075.0
55	175495.5
56	181844.5
57	180349.0
58	171127.0
59	168991.0
60	167865.0
61	164842.0
62	156544.0
63	144243.0
64	132371.0
65	119646.0
66	153825.5
67	193392.0
68	145059.5
69	96775.0
70	90426.0
71	83070.0
72	72368.5
73	63419.5
74	56684.5
75	46094.0
76	37948.0
77	30193.0
78	25059.0
79	23231.0
80	20061.0
81	14972.0
82	10314.5
83	7860.0
84	6504.5
85	5326.0
86	4460.5
87	3558.0
88	2410.0
89	1670.5
90	1082.5
91	537.0
92	337.5
93	240.5
94	120.0
95	21.0
96	25.0
97	25.0
98	322.0
99	619.0
100	619.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	22096.0
25	21507.0
26	21164.0
27	21285.0
28	22517.0
29	21467.0
30	23229.0
31	23439.0
32	24007.0
33	24216.0
34	22735.0
35	22355.0
36	24268.0
37	22743.0
38	21353.0
39	22129.0
40	23474.0
41	28780.0
42	27501.0
43	24252.0
44	27653.0
45	44574.0
46	56099.0
47	127174.0
48	457730.0
49	905017.0
50	897939.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.697109419268854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.67421644984088	5.885700653275505
2	13.23933810478612	2.3028794427204087
3	5.658485826074687	1.4763741113026037
4	2.9389334214669764	1.0224090216977793
5	1.8056915910278466	0.7852148672311423
6	1.1783561917390426	0.6148975640656441
7	0.8764758545720308	0.533596448739209
8	0.6520371612857807	0.45366708297055114
9	0.4838473786997195	0.37872662353000874
>10	3.450984433734073	6.031396157870658
>50	0.5786943393734193	3.5292513426624463
>100	0.7750645959982921	16.69120034371801
>500	0.5636482701610267	35.86422423730531
>1k	0.11921103044473462	14.017822037724956
>5k	0.0023147772901890213	1.2369357303382893
>10k+	0.002700573505220525	9.17570433484745
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	88294	2.9621871082090365	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA	71073	2.3844374967918642	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	62959	2.112219835387826	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC	15715	0.5272246178166694	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	12493	0.4191293127829241	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAG	11609	0.3894718796203446	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	11325	0.3799439259798779	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	7761	0.26037481761852826	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	7164	0.24034598549402605	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG	5636	0.1890829109777123	No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG	5635	0.18904936184517546	No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA	5443	0.18260792839809936	No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGG	5226	0.17532776663760194	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	4786	0.16056614832138594	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	4553	0.15274920044029883	No Hit
CTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTTGA	3906	0.1310429116889539	No Hit
TCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA	3561	0.11946846096373909	No Hit
CTCTTTCCCTAAGCAGCCTGAGGTGATCTGTGAAAATGGTTCGCTATTC	3506	0.1176232586742121	No Hit
TTTCTTGCTGCAGCAACGCGAGTGGGAGCACCAGGATCTCGGGCTCGGA	3400	0.1140670506253055	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	3365	0.1128928309865156	No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT	3327	0.11161796395011511	No Hit
AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCTCGGA	3227	0.10826305069642968	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC	3178	0.1066191432021238	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA	3069	0.10296228775560665	No Hit
AGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGG	3056	0.10252614903262754	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.026235421643820266	0.0	0.0	0.0	0.0
2	0.047841062997554605	0.0	0.0	0.0	0.0
3	0.06471627666359245	0.0	0.0	0.0	0.0
4	0.08477865792063148	0.0	0.0	0.0	0.0
5	0.10853144375672451	0.0	0.0	0.0	0.0
6	0.14667680745112813	0.0	0.0	0.0	0.0
7	0.18881451791741746	0.0	0.0	0.0	0.0
8	0.25316175412310454	0.0	0.0	0.0	0.0
9	0.2974466090717525	0.0	0.0	0.0	0.0
10	0.34404635416544355	0.0	0.0	0.0	0.0
11	0.3872576368729122	0.0	0.0	0.0	0.0
12	0.4439556708601964	0.0	0.0	0.0	0.0
13	0.5008549996427017	0.0	0.0	0.0	0.0
14	0.5565801087864172	0.0	0.0	0.0	0.0
15	0.6208266975944936	0.0	0.0	0.0	0.0
16	0.6797725234617471	0.0	0.0	0.0	0.0
17	0.7449920371133923	0.0	0.0	0.0	0.0
18	0.8107818860181641	0.0	0.0	0.0	0.0
19	0.878182093284705	0.0	0.0	0.0	0.0
20	0.9500778843111843	0.0	0.0	0.0	0.0
21	1.0143580222517976	0.0	0.0	0.0	0.0
22	1.074041929034862	0.0	0.0	0.0	0.0
23	1.131679338733178	0.0	0.0	0.0	0.0
24	1.1869683091539143	0.0	0.0	0.0	0.0
25	1.2418546899842084	0.0	0.0	0.0	0.0
26	1.2990559609595453	0.0	0.0	0.0	0.0
27	1.3505203302710804	0.0	0.0	0.0	0.0
28	1.4054067111013744	0.0	0.0	0.0	0.0
29	1.4696868490419877	0.0	0.0	0.0	0.0
30	1.5301423858733996	0.0	0.0	0.0	0.0
31	1.5904301770421274	0.0	0.0	0.0	0.0
32	1.6462559335834532	0.0	0.0	0.0	0.0
33	1.7010416670161368	0.0	0.0	0.0	0.0
34	1.769280602596099	0.0	0.0	0.0	0.0
35	1.8256431452580146	0.0	0.0	0.0	0.0
36	1.8886148670296907	0.0	0.0	0.0	0.0
37	1.945782588872491	0.0	0.0	0.0	0.0
38	1.9934223570748244	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTC	10	0.008717918	135.31743	44
GTCAATC	135	0.0	130.30568	44
ATTCACG	90	0.0	127.799805	44
CTATCAT	7310	0.0	121.711655	44
ACTACCC	140	0.0	120.819145	44
TAAGCCA	1470	0.0	115.98638	44
ACCCGAT	115	0.0	111.783966	44
CGTTCGG	20	5.088909E-4	101.488075	44
AATCGCA	910	0.0	95.168304	44
CCGGCAT	50	3.3833203E-10	94.722206	44
TATGGGA	135	0.0	90.211624	44
TAGAGTC	155	0.0	87.30157	44
GGTAAAG	195	0.0	86.74194	44
CGACGGG	785	0.0	84.46566	44
ACTTCTA	1785	0.0	82.63082	44
AATCACG	115	0.0	82.367134	44
CCCATAC	1760	0.0	79.9603	44
TCCGTAA	195	0.0	79.80259	44
TTAGTAG	165	0.0	77.910034	44
CAACGAA	275	0.0	76.26983	44
>>END_MODULE