FastQCFastQC Report
Tue 24 May 2016
ERR841249.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841249.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2819339
Sequences flagged as poor quality0
Sequence length24-50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC840762.982117439584243No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA606322.150575010667394No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA472761.6768469488770241No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT449271.5935295471740007No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA112040.39739811352944787No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC110950.393531959086864No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC78270.2776182644229729No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA69460.24636980512098755No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAG62800.22274724678373192No Hit
ATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCACTC54410.19298849836787985No Hit
AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCTCGGA52580.18649761522115646No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG52430.185965575618966No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC50810.18021954791530923No Hit
ATGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT43180.15315646681722206No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAAC41330.14659464505687325No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG40930.14517587278436542No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC39380.1396781302283975No Hit
CTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCT39310.13942984508070863No Hit
ATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATGACC38520.13662776984250563No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG37730.13382569460430263No Hit
ATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCACTC37220.13201675995685513No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC36710.1302078253094076No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC36660.13003047877534415No Hit
TGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCC36180.12832795204833475No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT34790.12339771840136997No Hit
AGACCATCCAAGAAGACAGTGCAGCCACCTCCGAGAGCCTGGATGTGAT33080.11733246693639893No Hit
CCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG32320.11463679961863402No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGG30990.10991938181254542No Hit
CTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTTGA30660.10874889468772644No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA30260.1073301224152186No Hit
AGTGCGTTACTTACCTCGACTCTTAGCTTGTCGGGGACGGTAACCGGGA29560.10484727093832988No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG28970.10275458183638081No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG28960.1027191125295681No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT28920.10257723530231731No Hit
CTCTTCTGAGGATCCGGCAAGATGGCAGAAGTAGAGCAGAAGAAGAAGC28800.10215160362056497No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC28370.10062642342761902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATA800.0130.4249644
CGTAATG850.0130.4249644
CACGATA750.0130.4249444
TAAGCCA55050.0122.48810644
GGGTAAT800.0122.2733944
AGGTAAT1250.0114.77396444
GCAATCC1050.0111.7928244
ACTTCTA14150.0109.2251344
TATAACG1200.0103.2530844
CTATCAT59650.0101.8910844
CGACGGG7850.0101.3493344
ACCACTC12450.098.9972544
GGTATTA950.096.1025944
ATACGTT950.096.1025944
TTAGACT1250.093.9059744
GTGCACA1000.091.2974744
GCCTCGA1150.090.7304144
ATCCCGG850.084.3926244
AATCGCA11850.083.6480744
CTACCGC950.082.3736544
Produced by FastQC (version 0.11.5)