##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841249.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2819339
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.64696476727347	39.0	37.0	39.0	32.0	39.0
2	37.221060681244786	39.0	37.0	39.0	34.0	39.0
3	37.505994135504814	39.0	37.0	39.0	34.0	40.0
4	37.978223973775414	39.0	37.0	40.0	34.0	41.0
5	38.46582940185625	40.0	38.0	41.0	34.0	41.0
6	38.40986947649786	40.0	38.0	41.0	34.0	41.0
7	38.3906983161656	40.0	38.0	41.0	34.0	41.0
8	38.283429200958096	40.0	37.0	41.0	34.0	41.0
9	38.22456646753015	40.0	37.0	41.0	34.0	41.0
10	38.18105520478382	40.0	37.0	41.0	34.0	41.0
11	37.885006733847895	40.0	37.0	41.0	33.0	41.0
12	38.14388088839264	40.0	37.0	41.0	33.0	41.0
13	38.260744096399904	40.0	37.0	41.0	34.0	41.0
14	38.251413540549756	40.0	37.0	41.0	34.0	41.0
15	38.16104767819691	40.0	37.0	41.0	34.0	41.0
16	38.21376641829876	40.0	37.0	41.0	34.0	41.0
17	38.15040830492537	40.0	37.0	41.0	33.0	41.0
18	38.1908383489889	40.0	37.0	41.0	34.0	41.0
19	38.11557035177395	40.0	37.0	41.0	33.0	41.0
20	38.01498046173234	40.0	37.0	41.0	33.0	41.0
21	37.95727934810252	40.0	36.0	41.0	33.0	41.0
22	37.89974210267017	40.0	36.0	41.0	33.0	41.0
23	37.78349570590837	40.0	36.0	41.0	33.0	41.0
24	37.56137449238988	39.0	35.0	41.0	32.0	41.0
25	37.55173276835916	39.0	35.0	41.0	32.0	41.0
26	37.54362992016676	39.0	35.0	41.0	33.0	41.0
27	37.465800453716795	39.0	35.0	41.0	32.0	41.0
28	37.4523052374332	39.0	35.0	41.0	32.0	41.0
29	37.42358249236966	39.0	35.0	41.0	32.0	41.0
30	37.315340977464906	39.0	35.0	41.0	32.0	41.0
31	37.19447619111583	39.0	35.0	41.0	32.0	41.0
32	37.14728122261801	39.0	35.0	41.0	32.0	41.0
33	37.0471561608228	39.0	35.0	41.0	31.0	41.0
34	36.95978857559811	38.0	35.0	41.0	31.0	41.0
35	36.94876290743366	38.0	35.0	40.0	31.0	41.0
36	36.885016870354825	38.0	35.0	41.0	31.0	41.0
37	36.80380813984215	38.0	35.0	40.0	31.0	41.0
38	36.647908538839395	38.0	35.0	40.0	31.0	41.0
39	36.620585347236855	38.0	35.0	40.0	31.0	41.0
40	36.5381989854218	38.0	35.0	40.0	31.0	41.0
41	36.4063049336445	37.0	34.0	40.0	31.0	41.0
42	36.226138139351335	37.0	34.0	40.0	30.0	41.0
43	36.17734179317785	37.0	34.0	40.0	30.0	41.0
44	36.14849327823916	37.0	34.0	40.0	31.0	41.0
45	36.00630575994578	36.0	34.0	40.0	30.0	41.0
46	35.79187037884188	36.0	34.0	40.0	30.0	41.0
47	35.757539560135164	36.0	34.0	40.0	30.0	41.0
48	35.78817803422584	36.0	34.0	40.0	30.0	41.0
49	36.147561374756876	37.0	34.0	40.0	30.0	41.0
50	36.594060100046136	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	313.0
15	2851.0
16	2774.0
17	60.0
18	2.0
19	1.0
20	3.0
21	19.0
22	46.0
23	149.0
24	394.0
25	975.0
26	2208.0
27	4750.0
28	10001.0
29	19679.0
30	35921.0
31	60681.0
32	97042.0
33	150210.0
34	221890.0
35	220601.0
36	290377.0
37	328074.0
38	376727.0
39	476200.0
40	517391.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	40.52425054241438	22.587067394165796	36.88868206341983
2	24.72973274941396	22.652756550382907	28.99254045008422	23.62497025011891
3	28.472631350823725	20.26595595634296	23.756845132848515	27.504567559984807
4	23.945648253012497	23.866516229513373	26.348693789572664	25.83914172790147
5	22.679145714651554	23.889464871021186	26.052099446004895	27.37928996832236
6	24.648543506119697	23.52565619104336	21.584704783639	30.24109551919794
7	27.764486640308245	21.830471610544176	20.595288470098843	29.80975327904874
8	23.69456103008542	21.837565471906714	28.049588928468694	26.418284569539175
9	27.174277374944978	24.23968880648975	24.8572449074056	23.728788911159672
10	33.99080422751574	21.374017101171585	21.218732475945604	23.41644619536707
11	25.365626481951974	21.795073242345104	26.13332415860597	26.705976117096952
12	27.40199032468249	22.829358229003326	24.401925415851018	25.36672603046317
13	26.29485138183099	22.029135198002084	22.462995758934984	29.213017661231945
14	22.765265191592782	22.69829914033041	19.4846735351797	35.05176213289711
15	23.96788750838406	27.491408447157294	24.583918429106966	23.956785615351684
16	23.951678035170655	28.56790190892262	20.380273532200277	27.100146523706442
17	22.14327542732534	32.49900065228055	20.14138775081677	25.216336169577335
18	23.789228609968507	23.544135699892777	20.092014475733496	32.57462121440522
19	27.893062877504267	22.447460202551024	20.039910064025644	29.619566855919068
20	24.90037558448984	21.869736133185828	22.18207884897843	31.0478094333459
21	25.00958558016613	27.612536129922656	21.292437695502386	26.085440594408833
22	26.128358455652194	22.65956665729095	24.035456537862242	27.176618349194616
23	30.64008975153396	22.467890523275138	25.231729848734048	21.660289876456858
24	25.690738148197152	21.85384588373374	25.52843060022225	26.92698536784686
25	25.70993089206223	27.850149026933384	20.821211952431344	25.618708128573044
26	25.60644564053247	28.27462663807756	24.676196689427186	21.442731031962786
27	24.530408913799967	25.094820145083606	23.71468943436809	26.66008150674834
28	23.9491078812668	21.684637696809137	23.887309474673415	30.478944947250646
29	25.863929119012674	23.258752142614796	25.236335512064258	25.64098322630828
30	24.044097853869392	25.11244219599137	23.657571441107763	27.185888509031475
31	23.559114665245808	29.427503972842512	24.05876560732906	22.954615754582623
32	25.781976892989306	23.810699563694833	24.031449201612734	26.375874341703128
33	24.434447193435417	20.596226535413525	27.712220374097267	27.25710589705379
34	23.502560348894647	26.082729324651783	28.36636233786657	22.048347988587
35	21.778444952196406	22.961925288464656	22.019237148107067	33.240392611231876
36	22.302025997450013	21.888857791460648	29.546366265509842	26.2627499455795
37	21.25254901008956	27.981739907603398	22.74184815905588	28.02386292325116
38	24.41810049791716	28.66521038094746	22.23300124390524	24.683687877230142
39	27.700591946506165	27.158298978703442	22.545210957967424	22.59589811682297
40	23.78495302856442	27.198623501869918	24.54586663791614	24.470556831649517
41	26.66028075143269	28.113224538552338	22.37555239041741	22.850942319597557
42	23.336606288717018	31.202206333026126	21.5850117920062	23.876175586250657
43	25.26346560885718	23.171570054387065	24.893100720501142	26.67186361625461
44	25.94672717178147	23.236196509945916	22.05404795318046	28.763028365092158
45	22.464484752097867	24.0570089350123	27.632607594257163	25.84589871863267
46	19.914848346223817	27.825696564783982	29.421832611679093	22.837622477313104
47	24.668624611913522	29.97132987940374	24.447460103443124	20.912585405239614
48	27.060276230047215	25.990967322744225	20.61309077364654	26.33566567356202
49	21.19889753841662	25.582724648328416	21.415704779531232	31.80267303372374
50	17.150991867859503	30.501971976227736	26.482261259712885	25.864774896199876
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2419.0
1	2269.0
2	2491.5
3	2804.5
4	2627.0
5	2217.0
6	1849.0
7	2095.5
8	2350.0
9	2158.0
10	2108.5
11	2126.5
12	2167.5
13	2700.5
14	3280.5
15	4467.5
16	5624.5
17	6963.5
18	8726.5
19	8464.5
20	7110.0
21	9907.5
22	13933.0
23	19145.0
24	24680.0
25	23953.0
26	21694.0
27	23897.5
28	28517.5
29	35901.5
30	43909.5
31	55236.5
32	66741.0
33	68056.0
34	76746.5
35	90956.0
36	95715.0
37	97444.5
38	94601.0
39	84311.5
40	79364.5
41	94356.0
42	111078.5
43	141338.5
44	173418.5
45	164671.5
46	156389.0
47	145929.0
48	139651.0
49	170586.0
50	235788.5
51	262251.0
52	205734.0
53	169600.5
54	170268.0
55	164481.0
56	168208.0
57	173791.5
58	164564.5
59	158039.5
60	160014.5
61	156393.5
62	141939.0
63	130395.5
64	124592.0
65	114169.0
66	134613.0
67	160411.0
68	125857.5
69	87956.5
70	77411.5
71	70582.5
72	62457.5
73	53557.5
74	47906.5
75	41139.5
76	36559.0
77	28848.0
78	21808.0
79	18141.0
80	13442.5
81	10373.5
82	8881.0
83	7536.5
84	6501.0
85	4741.0
86	2819.0
87	1566.0
88	975.5
89	790.0
90	756.5
91	607.0
92	432.0
93	358.5
94	239.0
95	91.5
96	53.5
97	54.0
98	240.0
99	424.5
100	423.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	18503.0
25	20290.0
26	19793.0
27	19582.0
28	20780.0
29	19670.0
30	20650.0
31	23215.0
32	22354.0
33	20991.0
34	20819.0
35	20132.0
36	18137.0
37	19664.0
38	19329.0
39	20403.0
40	18744.0
41	20806.0
42	23281.0
43	22262.0
44	25606.0
45	34403.0
46	61131.0
47	124873.0
48	465177.0
49	814352.0
50	884392.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.507022242871413
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.68212781025997	5.757733667267475
2	13.45158962011379	2.2886594420057245
3	5.5395822403228046	1.4137604800392465
4	2.912700535963754	0.9911363298506856
5	1.7724694487913182	0.7539218512838889
6	1.2021791226932024	0.613617872200003
7	0.8259582866639884	0.4918511861434159
8	0.6655897239789453	0.45297492692124264
9	0.499884209437451	0.38272734796901287
>10	3.4949082528413284	5.977706343248294
>50	0.509915776909387	3.08105356787852
>100	0.7307126388366555	15.00079025918355
>500	0.5661564858489252	35.61532340200914
>1k	0.1408100752148173	16.50114571766541
>5k	0.0029161849896559796	1.4911764797704714
>10k+	0.00249958713399084	9.186421126563928
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	84076	2.982117439584243	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA	60632	2.150575010667394	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	47276	1.6768469488770241	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	44927	1.5935295471740007	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	11204	0.39739811352944787	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	11095	0.393531959086864	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC	7827	0.2776182644229729	No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA	6946	0.24636980512098755	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAG	6280	0.22274724678373192	No Hit
ATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCACTC	5441	0.19298849836787985	No Hit
AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCTCGGA	5258	0.18649761522115646	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	5243	0.185965575618966	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC	5081	0.18021954791530923	No Hit
ATGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	4318	0.15315646681722206	No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAAC	4133	0.14659464505687325	No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG	4093	0.14517587278436542	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	3938	0.1396781302283975	No Hit
CTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCT	3931	0.13942984508070863	No Hit
ATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATGACC	3852	0.13662776984250563	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG	3773	0.13382569460430263	No Hit
ATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCACTC	3722	0.13201675995685513	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC	3671	0.1302078253094076	No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC	3666	0.13003047877534415	No Hit
TGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCC	3618	0.12832795204833475	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	3479	0.12339771840136997	No Hit
AGACCATCCAAGAAGACAGTGCAGCCACCTCCGAGAGCCTGGATGTGAT	3308	0.11733246693639893	No Hit
CCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG	3232	0.11463679961863402	No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGG	3099	0.10991938181254542	No Hit
CTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTTGA	3066	0.10874889468772644	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	3026	0.1073301224152186	No Hit
AGTGCGTTACTTACCTCGACTCTTAGCTTGTCGGGGACGGTAACCGGGA	2956	0.10484727093832988	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	2897	0.10275458183638081	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG	2896	0.1027191125295681	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	2892	0.10257723530231731	No Hit
CTCTTCTGAGGATCCGGCAAGATGGCAGAAGTAGAGCAGAAGAAGAAGC	2880	0.10215160362056497	No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC	2837	0.10062642342761902	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.032454415733616994	0.0	0.0	0.0	0.0
2	0.072144570057024	0.0	0.0	0.0	0.0
3	0.09048220167918793	0.0	0.0	0.0	0.0
4	0.11914140158384642	0.0	0.0	0.0	0.0
5	0.1487582727724477	0.0	0.0	0.0	0.0
6	0.19642902112871138	0.0	0.0	0.0	0.0
7	0.23750247841781355	0.0	0.0	0.0	0.0
8	0.3054616702709394	0.0	0.0	0.0	0.0
9	0.3527777255590761	0.0	0.0	0.0	0.0
10	0.4018317768810349	0.0	0.0	0.0	0.0
11	0.44740983613534946	0.0	0.0	0.0	0.0
12	0.4981664141843177	0.0	0.0	0.0	0.0
13	0.5482845447106574	0.0	0.0	0.0	0.0
14	0.5998214475095048	0.0	0.0	0.0	0.0
15	0.6607222473069042	0.0	0.0	0.0	0.0
16	0.7194948886955418	0.0	0.0	0.0	0.0
17	0.7844391894695885	0.0	0.0	0.0	0.0
18	0.8425024447219721	0.0	0.0	0.0	0.0
19	0.9053895257008824	0.0	0.0	0.0	0.0
20	0.9656164086688405	0.0	0.0	0.0	0.0
21	1.0279359807387476	0.0	0.0	0.0	0.0
22	1.079366475617157	0.0	0.0	0.0	0.0
23	1.1333862298928934	0.0	0.0	0.0	0.0
24	1.1820501188399124	0.0	0.0	0.0	0.0
25	1.2357506493543344	0.0	0.0	0.0	0.0
26	1.2875713066076835	0.0	0.0	0.0	0.0
27	1.333184835168811	0.0	0.0	0.0	0.0
28	1.3827354567861474	0.0	0.0	0.0	0.0
29	1.4370389655163853	0.0	0.0	0.0	0.0
30	1.4909523118716834	0.0	0.0	0.0	0.0
31	1.5430921928863468	0.0	0.0	0.0	0.0
32	1.5924299986628072	0.0	0.0	0.0	0.0
33	1.6436122083935276	0.0	0.0	0.0	0.0
34	1.7087693250084506	0.0	0.0	0.0	0.0
35	1.7591002713756665	0.0	0.0	0.0	0.0
36	1.8110273365494536	0.0	0.0	0.0	0.0
37	1.8640184809276217	0.0	0.0	0.0	0.0
38	1.9120439223520123	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAATA	80	0.0	130.42496	44
CGTAATG	85	0.0	130.42496	44
CACGATA	75	0.0	130.42494	44
TAAGCCA	5505	0.0	122.488106	44
GGGTAAT	80	0.0	122.27339	44
AGGTAAT	125	0.0	114.773964	44
GCAATCC	105	0.0	111.79282	44
ACTTCTA	1415	0.0	109.22513	44
TATAACG	120	0.0	103.25308	44
CTATCAT	5965	0.0	101.89108	44
CGACGGG	785	0.0	101.34933	44
ACCACTC	1245	0.0	98.99725	44
GGTATTA	95	0.0	96.10259	44
ATACGTT	95	0.0	96.10259	44
TTAGACT	125	0.0	93.90597	44
GTGCACA	100	0.0	91.29747	44
GCCTCGA	115	0.0	90.73041	44
ATCCCGG	85	0.0	84.39262	44
AATCGCA	1185	0.0	83.64807	44
CTACCGC	95	0.0	82.37365	44
>>END_MODULE